1.Application of Filamentous Phage Display Technology in Parasitology
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(04):-
This review focuses on the application of phage display technology in the prevention,diagnosis and treatment of parasitic diseases. It covers: ① an introduction of phage display technology,filamentous phage and the advantage of carrier. ② construction of phage antibody libraries of parasites,epitope mapping and mimotope,and their potential application in the development of novel diagnostic reagents and vaccines.
2.Effect of dexamethasone on vascular endothelial growth factor in cerebrospinal fluid of rabbits with bacterial meningitis
Junju LIU ; Yan XUE ; Xuepeng GUO
Chinese Pediatric Emergency Medicine 2010;17(3):242-243,247
Objective To investigate the effects of dexamethasone (DEX) on vascular endothelial growth factor( VEGF) in cerebrospinal fluid of rabbits with bacterial meningitis. Methods A total of 36 rabbits were assigned to study,which were randomly divided into meningitis model group (MOD) .dexametha-sone-treated group (DEXT) and control group( CON). CSF was sampled for determining at 6 h, 12 h and 24 h after injection of E. coli suspension. The concentration of VEGF in every CSF sample was determined quantitatively by ELISA. Results There were higher concentrations of CSF VEGF at6h,12h and 24hin M0D(( 1219 ±176) ng/L,( 1343 ±160) ng/L,(981 ±134) ng/L) than that in CON( (374 ±172) ng/L, (370 ± 169) ng/L,(367 ± 171) ng/L) (P<0.01). There was higher brain water content in M0D( (80.8 ± 0.5) % ) than that in CON( (80.0 ± 0.5) % ) (P < 0.01). There was positive correlation between the brain water content and the concentration of CSF VEGF at 24 h( r - 0.919,P < 0.01). Compared with MOD, the concentrations of CSF VEGF in DEXT at 6 h, 12 h,24 h ((941 ±147) ng/L, (1083 ± 123) ng/L, (825 ± 66) ng/L) were decreased significantly(P <0.05), the brain water content was less ((80.4 ±0.5) %) (P < 0.05). Conclusion The secretion of VEGF markedly increases in the pathological process of bacterial meningitis. VEGF contributes to the damage of blood brain barrier and the formation of brain edema. DEX can decrease the degree of brain edema by suppressing the generation of VEGF and lightening the damage of blood brain barrier.
3.Protective Effects of Cordyceps Sinensis from Different Habitats on Acute Injury of Hepatic Tissue Induced by Carbon Tetrachloride
Chen WEI ; Guo XIA ; Du GUANG ; Gong XUEPENG ; Sun YUANYUAN
China Pharmacist 2015;(8):1277-1279
To study and compare the protective effects of Cordyceps sinensis from different habitats on acute injury of hepatic tissue induced by carbon tetrachloride ( CCl4 ) . Methods:The rat model of acute injury of hepatic tissue induced by CCl4 was established. The biochemical indicators of alanine aminotranferease ( ALT) and aspartate aminotransferease ( AST) in the serum and superoxide dismutase ( SOD) and L-glutathione( GSH) in the liver tissue were detected, and the liver tissue HE staining was used for the histopathology determination. Results:Compared with those in the model group, the levels of AST and ALT in the serum were sig-nificantly reduced, the levels of SOD and GSH in the liver tissue were notably increased, and the necrosis of liver cells was improved in Cordyceps sinensis groups at low, medium and high dose from different habitats (P<0. 05), however, the differences between the two different habitats were not significant (P>0. 05). Conclusion: Cordyceps sinensis from Qinghai and Tibet shows some positive differences in the protective effect on the acute chemical liver injury induced by CCl4 in rats,however, the differences are not remarka-ble.
4.Effects of dead box 1 gene on cell apoptosis, proliferation and cell cycle of neuroblastoma cells
Jianhua LI ; Yufeng LIU ; Jiaqin WANG ; Xuepeng GUO
Chinese Journal of Applied Clinical Pediatrics 2016;31(7):527-530
Objective To explore the effects of dead box 1 (DDX1) gene on cell apoptosis,proliferation and cell cycle of neuroblastoma(NB) cells.Methods SK-N-BE(2)/blank,SK-N-BE(2)/shV and SK-N-BE (2)/shDDX1 cells were seeded in 96-well plates,which grew in good condition and in the logarithmic growth phase,5 000 cells were inoculated in each well and 5 repeated holes were set.Cell count kit 8 (CCK-8) was used to detect the cell number at 12 h,24 h,36 h,48 h,and the average was calculated.The time (hour) was set as abscissa,the optimal density (A) value at 450 nm was set as vertical axis,and the growth curves of these 3 cells were drawn to investigate the effects of DDX1 on the proliferation of NB cells.After 24 h,flow cytometry (PI staining) was used to detect the apoptosis of SK-N-BE (2)/blank,SK-N-BE (2)/shV and SK-N-BE (2)/shDDX1 cell lines to observe the effects of DDX1 on the apoptosis of NB cells.After 24 h,flow cytometry (PI staining) was used to detect the proportion of SK-N-BE(2)/blank,SK-N-BE(2)/shV and SK-N-BE(2)/shDDX1 cells at G1,S,M,G2 stage to observe the effects of DDX1 on the cell cycle of NB cells.Results SK-N-BE (2)/shDDX1 cell proliferation was significantly lower than SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,that was to say,DDX1 knockdown reduced the cell proliferation of NB.According to the flow cytometry results,the total average apoptosis rate was 5.28% in SK-N-BE (2)/shV cells,and the total average apoptosis rate was 9.99 % in SK-N-BE (2)/shDDX1 cells.The number of apoptotic SK-N-BE (2)/shDDX1 cells was significantly higher than the number of SK-N-BE (2)/blank and SK-N-BE (2)/shV cells,which indicated that DDX1 knockdown increased tumor cell apoptosis of NB.Compared with SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,the cell cycle of SK-N-BE(2)/shDDX1 cells was arrested,and the proliferation was affected.Conclusions After DDX1 expression is inhibited,the cell cycle of NB cells are affected,the cell apoptosis is increased,and the cell proliferation is reduced.
5.Effects of dead box 1 gene on invasion, migration and drug resistance capability of neuroblastoma cells
Jianhua LI ; Yufeng LIU ; Jiaqin WANG ; Xuepeng GUO
Chinese Journal of Applied Clinical Pediatrics 2016;31(8):616-619
Objective To explore the effects of dead box 1 (DDX1) gene on invasion,migration and drug resistance capability of neuroblastoma(NB) cells.Methods According to the virus drop degree,the appropriate amount of target virus(Lenti-DDX1-MIR virus liquid,drop degrees 1012 TU/L) and negative control virus(Lenti-EGFP virus liquid,drop degrees 3 × 1011 TU/L) (multiplicity of infection was 10) were added into 2 hole cells,respectively.SK-N-BE(2)/blank,SK-N-BE(2)/shV and SK-N-BE(2)/shDDX1 cells which grew in good condition were cultured.Transwell chamber was used to detect the invasion,and cell staining was made with crystal violet.The researchers calculate 5 field counting in each small room and calculate the average cell invasion rate.Transwell chamber was used to detect the migration,and cell staining was made with crystal violet.The 570 nm absorbance values was tested with enzyme linked immunosorbent assay (ELISA) reader,and cell migration was calculated.The researchers used 50 mg Cisplatin,solution 10 g/L mother liquor standby with 5 mL dimethyl sulphoxide,and 50 mg Doxorubicin,solution 1 g/L mother liquor standby with PBS.Drugs were added to the cell culture plate,and Doxorubicin final concentration was 1.0 mg/L,and Cisplatin final concentration was 2.5 mg/L,and photographic record was documented after drug treatment for 24 h.Cell Count Kit-8 (CCK-8) was used to detect the drug sensitivity to NB cells to Doxorubicin and Cisplatin.Results Transwell results showed that,cell invasion concentration in SK-N-BE (2)/shDDX1 was 60% compared with SK-N-BE (2)/blank and SK-N-BE (2) /shV;Crystal violet staining showed that cell invasion of SK-N-BE(2)/shDDX1 was significantly weaker than that of SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,that is to say,DDX1 knockdown reduced the cell invasion of NB.Transwell results showed that,cell migration concentration in SK-N-BE(2)/shDDX1 was 50% compared with SK-N-BE(2)/blank and SK-N-BE(2)/shY;Crystal violet staining showed that cell migration of SK-N-BE(2)/shDDX1 was significantly weaker than that of SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,that is to say,DDX1 knockdown reduced the cell migration of NB.With DDX1 knockdown,24-h inhibition rate of SK-N-BE (2)/shDDX1 cell was 1.93 times of SK-N-BE (2)/shV cell with 1.0 mg/L Doxorubicin,24 h inhibition rate of SK-N-BE(2)/shDDX1 cell was 1.38 times of SK-N-BE(2)/shV cell with 2.5 mg/L Cisplatin.DDX1 knockdown could increase the Doxorubicin and Cisplatin drug sensitivity to NB cells.Conclusion DDX1 knockdown can decrease the cell invasion,migration and resistance capability of NB and increase the Doxorubicin and Cisplatin drug sensitivity of NB cells.
6.Expression of dead box 1 gene in neuroblastoma
Jianhua LI ; Yufeng LIU ; Jinghang ZHANG ; Jiaqin WANG ; Xuepeng GUO
Chinese Journal of Applied Clinical Pediatrics 2016;31(6):445-447
Objective To detect the expression of dead box 1(DDX1)gene in tumor tissue and pericarcino-matous tissue of clinical neuroblastoma(NB)samples,and explore the relationship between DDX1 and NB. Methods Five cases of pathological specimens in children with NB were chosen from Department of Pathology,the First Affi-liated Hospital of Xinxiang Medical University between January 2012 and December 2014. In the 5 cases,3 cases were male,2 cases were female,the age of 1 - 5 years old,average age(2. 1 ± 1. 6)years. The NB tissue and pericarcino-matous tissue(pericarcinomatous tissue was normal tissue which was at least 2 cm from the tumor tissue)of 5 children were collected and fixed in 40 g/ L formaldehyde solution. Then with the conventional dehydration,embedding,sectio-ning, dewaxing, hydration, antigen repair, add primary antibodies, secondary antibodies, diaminobenzine chromogenic. The expressions of DDX1 in tumor tissue and pericarcinomatous were observed with light microscopy and with semi - quantitative analysis.(1)Staining degree:no staining with 0 score;light staining with 1 score;medium staining with 2 scores;deeply staining with 3 scores.(2)Positive cells proportion:positive cells proportion ﹤ 10% with 0 score;positive cells proportion within 10% - 30% with 1 score;positive cells proportion within 31% - 60% with 2 scores;positive cells proportion ﹥ 61% with 3 scores. Final scores were a half of the sum of staining degree score and positive cells proportion score,final score within 0 -1. 0 with - ,1. 1 -2. 0 with + ,2. 1 - 3. 0 with + + ,3. 1 -5. 0 with + + + . Results DDX1 were expressed in NB and pericarcinomatous tissues,but visible DDX1 positive staining number more and deeper in NB,DDX1 positive staining number less and light in pericarcinomatous tissues. Five cases of pericarcinomatous tissues immunohistochemical semi - quantitative score were negative and final scores were 1. 0 score or less,the mean value was 0. 5 score. NB immunohistochemical semi - quantitative score were+or + +and final scores were 1. 5 score or higher,the mean value was 1. 8 scores,the expressions of DDX1 were sig-nificantly higher in NB than the pericarcinomatous tissues. Conclusions DDX1 is highly expressed in NB,which may contribute to the development of NB. This suggest DDX1 may serve as an oncogene and play a catalytic role in the de-velopment of NB,which provides a clinical evidence for the follow - up study.
7.Recent Advances in the Biomedical Application of Suparmolecular Hydrogels
Jiankun GUAN ; Xuepeng GONG ; Xia GUO ; Yuanyuan SUN ; Guang DU
Herald of Medicine 2017;36(7):770-773
Objective Supramolecular hydrogels were the hydrogels consisting of a solid 3D network with noncovalent bonds.Its unique properties such as biocompatibility,biodegradability,free sol-gel transformation and stable drug release ability make it widely exploited for various biomedical applications.This paper mainly focused on the use of supramolecular hydrogels in all types of biomedical application such as biosensor,cell culture,tissue engineering,gene engineering and drug delivery by research literature reviews.They hope that this focus review will contribute topromote the use of supramolecular hydrogels.
8.The Application of Real Time Three-Dimensional CT in Detection of SupraclavicularRegion Lymph node Metastasis of Esophageal Cancer
Jianying LIANG ; Xing GUO ; Xuepeng LI ; Xiumei CHENG ; Zhigang FENG ; Yaozhong GUO
Journal of Practical Radiology 2000;0(12):-
Objective To discuss real time three-dimensional reconstruction techniques of spiral CT in the detection of tracheoesophageal groove lymph node metastasis and its diagnostic value.Methods Spiral CT RT3D reconstruction of the supraclaviculrar region were applied to 45 patients with scanning and post-processing on the workstation.Mediastinum,color in chest,clip planes 3D images were obtained by adjusting the CT value threshold and encoding pseudo color techniques.Results RT3D images can correctly reveal the macroscopic morphology of lymphatic metastasis and it's pressure to the trachea and carotid artery.There were significant differennces of visualization capabilities of radiological signs among different 3D techniques.Conclusion With the utilization of multiple methods,RT3D reconstruction can play an important role in detecting tracheoesophageal groove lymph node metastasis of esophageal cancer.It is an important complement to the axial CT images and the basis of clinical treatment.
9.The effects of donor peripheral blood stem cell infusion in the prevention of relapse in high risk leukemia after haplotype hematopoietic stem cell transplantation
Yuan ZHANG ; Huiren CHEN ; Xiaodong LIU ; Xuepeng HE ; Jinxing LOU ; Zhi GUO
Chinese Journal of Internal Medicine 2011;50(6):492-495
Objective To explore the preventative effect of donor peripheral blood stem cell (PBSC) infusion mobilized by granulocyte colony-stimulating factor (G-CSF) for the relapsing patients with leukemia after haplotype hematopoietic stem cell transplantation ( HSCT) , as well as its therapeutic effect and safety. Methods G-CSF was given at 5-10 μg · kg-1 · d-1 to donor and PBSCs were obtained on day 5 and frozen and allotted for storing. PBSC infusion was given to all the 20 patients on day 90 after HSCT,and the second treatment was given to 4 patients on day 30 after the first infusion. The occurrence of graftversus-host disease ( GVHD) , relapse rate of high risk leukemia and long-term survival were evaluat after PBSC infusion. Results A total of 19 patients had acute GVHD after PBSC infusion for a median of 25 (12-60) months, 4 of them were ≥ degree Ⅲ. The cumulative incidence rate was 22.9%, and all of them accepted PBSC infusion twice. Thirteen patients had assessable chronic GVHD, 10 of them were restricted,and no one died of it. Nine patients died of relapse of leukemia. The remaining 11 patients survived leukemia free, including 4 with chronic myelogenous leukemia, 4 with acute myeloid leukemia (AML) , 1 with lymphoma-leukemia and 2 with myelodysplastic syndrome-AML (MDS-AML). Kaplan-Meier analysis showed the disease free survival rate of 2-year was 52. 5%. Conclusion The prophylactic donor PBSC infusion mobilizing with G-CSF is effective, safe and feasible for the relapse of leukemia.
10.Malignant blood disease great amount of application curative effect IL-2 observes after the chemotherapy
Zhi GUO ; Xiaodong LIU ; Xinjian RUAN ; Jinxin LOU ; Xuepeng HE ; Xiaohua TAN
Journal of Leukemia & Lymphoma 2008;17(2):123-124
Objective The clinical curative effect observed the empress of the chemotherapy of malignant blood disease big mount of an application IL-2. Methods The review analyzes 65 malignant chemotherapy be over of the blood patient's (treatment set) juniorses to go a big quantity IL-2 treatments,(matched control) choose random of 98 sufferer's chemotherapy be overs don't carry on any immunity treatment behind. Results With visit be over the covariance relapse a rate: the treatment set is 28 %, the matched control is 42 %; Medium exist a period: the treatment set is 44(0~80) months, the matched control is 21(0~80) months. Conclusion For the leukemia, lymphoid lump etc. malignant blood patient the chemotherapy gives after ending big quantity IL-2 immunity treatment is a kind of to cure a method effectively, after maying reduce a sufferer a chemotherapy of relapse a rate, also hope extension existence a period.