Objective:To investigate the curative effect of electroacupuncture on urine retention following cervical cancer operation. Methods: A treatment group was treated with electroacupuncture according to classification based on syndrome differentiation and a control group, by a conventional method. One course of treatment consisted of 5 days. Results: The curative rate and cure rate were 96.67% and 76.67%, respectively, in the treatment group and 70% and 36.67%, respectively, in the control group. There were significant differences in curative rate and cure rate between the two groups (P＜0.01). Conclusion:Electroacupuncture is a good method for treating urine retention subsequent to cervical cancer operation.

Objective To prepare the chitosan nanoparticles(particle-diameter

Objective To develop the clinical application of tandem mass spectrometry in earlystage diagnosis of patients with growth retardation.Methods Tandem mass spectrometry was used to quantitatively detect blood amino acids and acyl carnitine in 155 cases of children with growth retardation.The tandem mass spectrometry results,clinical symptoms,and treatment tracking results were analyzed.Results Ten patients with inborn metabolic absence of amino acids and acyl carnitine were confirmed including four cases of methylmalonic acidemia,one case of propionic acidemia,one case of medium-chain acyl-coenzyme A dehydrogenase deficiency,one case of ornithine transcarbamylase deficiency,and three cases of phenylketonuria.Conclusions Tandem mass spectrometry was helpful for some patients in etiologic diagnosis and therapeutic effect assessment of cerebral developmental retardation.

Objective To investigate the prevalence and risk factors of metabolic obesity with normal weight (MON W) in Han nationality adult population,and to identify the cut-off points of body fat percentage (％ BF) by dualenergy X-ray absorptiometry(DXA) for defining MONW.Methods Six hundred and sixty subjects (403 men and 257 women) with body mass index(BMI) ranged from 18.5 to 23.9 kg/m2 were recruited into this study.Waist circumference(WC),blood pressure (BP),BM1,and ％ BF were measured.Serum concentrations of glucose,triglycerides(TG),total cholesterol (TC),high-density lipoprotein-cholesterol (HDL-C),low-density lipoproteincholesterol(LDL-C),serum insulin,and high-sensitivity C-reactive protein (hsCRP) were tested.Those within the highest quartile in the homeostasis model assessment of insulin resistance (HOMA-IR) with a normal BM1 between 18.5 and 23.9 kg/m2 were classified as MONW.Results The prevalence of MONW was 11.34％ for men and 16.34％ for women amongst normal-weight subjects,there was no significant different between men and women in the prevalence(P＞0.05).Multivariable Logistic regression analysis showed that TG(OR =5.92,P=0.001),LDLC (OR =13.65,P =0.009),hsCRP (OR =2.09,P =0.011),WC (OR =2.35,P ＜ 0.01),％ BF (total,OR =3.65,P＜0.01) and ％ BF(abdominal,OR =26.07,P＜0.01) were the risk factors of MONW amongst men.For women,TG (OR =9.12,P＜0.01),LDL-C (OR =2.64,P =0.005),HDL-C (OR =0.020,P＜ 0.01),hsCRP (OR =2.15,P =0.039),WC (OR =1.32,P =0.045),％ BF(total,OR =1.73,P＜0.01) and ％ BF(abdominal,OR =8.43,P＜0.01) were the risk factors of MONW.The cut-off points of ％ BF for diagnosing MONW by ROC were ％ BF≥25％ for men,％ BF ≥35.0％ for women.Conclusion MONW is quite common in Han nationality adult population;.％ BF,WC,hsCRP and lipid profiles were independently associated with MONW ％ BF ≥25 ％ for men,％ BF≥35.0％ for women can be used as reference cut-off.point for diagnosing MONW.

Objective To investigate the clinicopathologic features and prognostic significance of BRCA1 and Ki-67 expression in breast cancer.Methods The expression levels of BRCA1 and Ki-67 were assayed by immunohistochemistry in 194 cases of breast cancer tissues.The correlations of BRCA1 and Ki-67 expression with patients' clinicopathologic features were also analysed.Results Low expression of BRCA1 was detected in the lymph node metastasis group,ER/PR negative group,and HER-2 positive group in 194 patients with breast cancer (P ＜ 0.05),as well as in the triple negative breast cancer (TNBC) group compared to non-TNBC group (P ＜0.05).High expression of Ki-67 was detected in patients with higher histological grade,negative ER/PR,and positive HER-2 (P ＜ 0.05).Furthermore,negative correlation was found between the expression of BRCA1 and Ki-67 (P ＜ 0.05).The combination of low expression of BRCAl and high expression of Ki-67 was mostly found in the patients with postmenopausal,lower histological grade,lymph node metastasis,negative ER/PR and positive HER-2 (P ＜ 0.05).However,there was no significant difference between TNBC and non-TNBC.Conclusion Joint detection of BRCA1 and Ki-67 might play an important role in predicting clinical outcomes of breast cancer,especially BRCA1 may be one of prognostic factors in TNBC.

0.05).The absorption of NCTD was a first-order process with passive diffusion mechanism.The absorption law in vivo was the same as in vitro,the correlation coefficient between them was 0.999 4.Conclusion NCTD-CS-NP could improve the absorption of NCTD in rat small intestine.NCTD is well absorbed at the superior and middle segments of intestine.The concentration of NCTD has no distinctive effect on the absorption kinetics.The release of drug in vitro and its uptake is well correlated.

Objective To investigate relationship of immunoglobulin concentrations (IgG,IgA,and IgM)and decrease of serum Mg2+ concentration in patients with severe traumatic brain injury (sTBI).Methods Sixty patients with sTBI were randomly divided into control group and study group.Intravenous injection of MgSO4 was given to study group.Levels of Mg2+ in serum and immunoglobulin (IgG,IgA,and IgM) in both groups were measured at 1,3,7,and 14 days after injury so as to analyze correlations of serum Mg2+ concentration with each humoral immune indices.Results IgG concentration at days 3 and 7 in study group were (10.79 ± 2.65) g/L and (10.2 1 ± 2.54) g/L respectively,higher than that in control group (P ＜ 0.05).IgM at days 3 and 7 in study group were (1.27 ± 0.32) g/Land (1.31 ± 0.25) g/L respectively,higher than that in control group (P ＜ 0.05).Difference of IgA level between the two groups was not significant.Level of Mg2 + was positively related to levels of IgM and IgG,but not to level of IgA.Pathological amelioration at day 14 between the two groups showed insignificant difference.Conclusion Intravenous replenishment of MgSO4 for patients with sTBI at acute phase can improve humoral immunosuppressive state,but can not ameliorate prognosis.

Objective To investigate the effect of neutrophil elastase (NE) inhibitor on the blood-brain barrier(BBB) permeabili-ty and hydrocephalus in rats with traumatic brain injury (TBI) .Methods 99 SD rats were randomly divided into the control group , the TBI group and the intervention group(dividing into 5 sub-groups:6 ,24 ,48 ,72 ,168 h) .The hydraulic impact model of rats was duplicated .Sivelestat sodium was given in the intervention group .The NE concentration in the brain tissue ,BBB permeability and brain water content were detected in each group and performed the comparative analysis .Results The NE concentration in the brain tissue ,BBB permeability and brain water content at each timepoint in the TBI group and the intervention groups were higher than those in the control group .The NE concentration at 24 ,48 ,72 ,168 h in the intervention group was lower than that in the TBI group .The BBB permeability and the brain water content at 48 ,72 ,168 h in the intervention group were lower than those in the TBI group(P<0 .05) .Conclusion Sivelestat sodium can inhibit the NE release in TBI rat brain tissue ,reduce the BBB permeability and the occurrence of hydrocephalus ,which indicating that sivelestat sodium has the protective effect on TBI secondary lesion in rat .

Chemotherapy completion rate can reflect the tolerance and compliance of patients to chemotherapy. Poor tolerance may result in delay or suspension of the comprehensive treatment plan, thus affect the efficacy of cancer treatment. Evaluating methods to improve the completion rate of chemotherapy and reduce the occurrence of delayed chemotherapy has gained increasing attention and is the significant area of study in the field of cancer treatment. Studies have shown that Chinese medicine combined with chemotherapy could improve the quality of life in patients with stage IIIB/IV non-small-cell lung cancer (NSCLC).

PURPOSE: Isocryptotanshinone (ICTS) is a natural bioactive product that is isolated from the roots of the widely used medical herb Salvia miltiorrhiza. However, few reports exist on the mechanisms underlying the therapeutic effects of ICTS. Here, we report that ICTS has anticancer activity and describe the mechanism underlying this effect. METHODS: The antiproliferative effect of ICTS was determined using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and clonogenic assays. The effect of ICTS on the cell cycle was measured using flow cytometry. Apoptosis was determined by Hoechst 33342 staining, DNA fragmentation assays, and Western blotting for apoptotic proteins. Finally, the effect of ICTS on mitogen-activated protein kinases (MAPKs) was determined by Western blotting. RESULTS: ICTS significantly inhibited proliferation of MCF-7 and MDA-MB-231 human breast cancer cells, HepG2 human liver cancer cells, and A549 human lung cancer cells in vitro. Among the tested cell lines, MCF-7 cells showed the highest sensitivity to ICTS. ICTS significantly inhibited colony formation by MCF-7 cells. Furthermore, exposure of MCF-7 cells to ICTS induced cell cycle arrest at the G1 phase and decreased mitochondrial membrane potential. Hoechst 33342 staining and Western blot analysis for apoptotic proteins suggested that ICTS induced apoptosis in MCF-7 cells. In addition, ICTS activated MAPK signaling in MCF-7 cells by inducing time- and concentration-dependent phosphorylation of JNK, ERK, and p38 MAPK. CONCLUSION: Our results suggest that ICTS inhibited MCF-7 cell proliferation by inducing apoptosis and activating MAPK signaling pathways.
Apoptosis* ; Blotting, Western ; Breast Neoplasms* ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Line ; DNA Fragmentation ; Flow Cytometry ; G1 Phase ; Hep G2 Cells ; Humans ; Liver Neoplasms ; Lung Neoplasms ; MCF-7 Cells* ; Membrane Potential, Mitochondrial ; Mitogen-Activated Protein Kinases ; p38 Mitogen-Activated Protein Kinases ; Phosphorylation ; Salvia miltiorrhiza