Objective Analysis of the characteristic preferences of high-level medical care talents to identify effective and targeted incentive plans for them.Methods Questionnaires were designed in view of characteristics of these talents for investigation in a tertiary hospital in Nanjng.The results were summarized in the main factor analysis method.Results Characteristic preferences as found in the summary are three main factors with distinctive significance,which are material needs,achievement needs and development needs,ranking 4.7 points,3.8 points and 3.9 points respectively.Conclusions These three characteristic preferences could be.sorted by ranking as achievement needs,material needs and development needs.Based on which,the corresponding incentive countermeasures are proposed.

Objective To study the clinical results of grafting of vascularized fibular and ilium to repair bone defects of limbs. Methods From January 1992 to January 2004, 49 cases of bone defects of limbs were treated with vascularized fibular grafting (33 cases) and vascularized iliac grafting (16 cases). Their limb functions were graded according to Ennekings system. The changes of the vascularized fibular and iliac grafts were evaluated radiographically according to the International Symposium on Limb Salvage. Results All the patients were followed up for 1 to 12 years. In the vascularized fibular group, the recovery rate for the operated limbs was 82.7%, the average healing time of the bone was 16.8 weeks and the healing rate of the bone was 87.9%. In the other group, the recovery rate of the operated limbs was 88%, the average healing time of the bone was 15.2 weeks and the healing rate of the bone was 93.8%. Conclusion The vascularized fibular graft can be adopted to repair long bone defects of lower limbs and distal radius, while the vascularized iliac graft can be adopted to repair the bone defects of upper limbs.

Purpose:To investigate the effect of Flk-1、LR P and MDR1 genes in the carcinogenesis and development of lung cancer. Methods:The expression of Flk-1、LRP and MDR1 geneproteins in primary lung tumors were studied immunohistochemically. Results:of the 70 lung cancers, 29 cases (49.2%) were positive for MDR1 expression in NSCLCs and 2cases (18.2%) in small cell lung carcinoma(S CLC); 41cases (69.5%)had overexpression of LRP in NSCLCs and 3cases (27.3%) in S CLCs, there was a statistically significant correlation (P

OBJECTIVE:To prepare a nickel-titanium alloy stent coated by anti-tuberculotic isoniazid and study the dissolution of isoniazid in vitro.METHODS:The polyurethane which was used as film-former was mixed with isoniazid before being coated uniformly on the stent,then the stend was torrefied and dried so that the drug layer alloy stent was obtained.The content and accumulated dissolution in vitro of isoniazid from the stent was determined by ultraviolet spectrophotometry.RESULTS:The linear range of isoniazid was 5.0～30.0 ?g?mL-1(r=0.999 9)and its recovery rate was 99.67%(RSD=1.06%).A high accumluated dissolution rate of isoniazid was achieved,over 60% within the first 30 minutes,followed by a sustained dissolution in the following 8 h.CONCLUSION:The preparation method of the stent is simple and the quality is controllable.

Objective For better recognition of the hereditary nonpolyposis colorectal cancer (HNPCC) with reference to its clinical characteristics, diagnosis and treatment [WT5”HZ]Methods [WT5”BZ] A survey of 7 families of HNPCC patients was made [WT5”HZ] Results [WT5”BZ]Among the 7 families, 21 persons had colorectal cancer with age ranging from 26 to 79 years (average 46 years) The total of the colorectal cancer foci in 21 cases were 31,among which 16 were located proximal to,10 distal to the splenic flexure and 5 without definite record 61 5%(16/26) of the colorectal cancer foci were located proximal to the splenic flexure Six patients had multifocal colorectal cancer, which accounted for 28 6%(6/21) of all cases [WT5”HZ] Conclusions [WT5”BZ] HNPCC patients and their family members should be followed up regularly for early diagnosis and treatment of the disease and for better prognosis

Objective: To investigate the expression of Cyclin E in colorectal carcinoma and its significance.Methods: The Cyclin E expression and the cell proliferation(PI,SPF)in 30 cases of colorectal carcinomas (including tumors and normal tissues distant to the tumors)were respectively assayed with Flow Cytometry (FCM), and the relationships between the Cyclin E expressive rate and clinical pathologic features were compared. Correlaction between the Cyclin E expressive rate and the cell proliferation(PI, SPF) was analyzed too.Results: The Cyclin E positive rate and the cell proliferation in colorectal carcinoma were significantly higher than those of the distal normal tissues. As for the Cyclin E positive rate and the cell proliferation,no significant differences were found among the subgroups divided according to malignance, lymphatic metastasis and the site of tumor. There was a significant correlation between the Cyclin E positive rate and the cell proliferation.Conclusion: The Cyclin E overexpression plays an important role in the onset of colorectal carcinoma.The Cyclin E expressive rate is not consistent with the general clinical pathologic features,but with the cell proliferation. The Cyclin E expressive rate may be one of the potential prognostic indicators in coloretal carcinoma.

Objective To evaluate the value of high frequency color Doppler ultrasound in diagnosis of malignant tumor in the parotid gland.Methods High frequency ultrasound was used to observe shape and internal echo texture of tumor,color Doppler flow imaging(CDFI) was added to observe blood flow signal,peak systolic velocity(PSV) and resistance index(RI) were measured by pulsed wave Doppler, and lymphonodus around parotid gland were examined.Results The features of ultrasonography included round,lobulated and irregular shape.Three types of boundary were clear but not smooth,unclear,irregular and invasive.Internal echo texture included low and irregualr echo,cystic echo,spot like and intensive echo,honey comb like echo.CDFI showed spet like,stick like blood flow signal.PSV and RI were 4.6 17.5 cm/s,and 0.69 1.14 .Eight cases were misdiagnosed for benign tumor,the correspondent rate was 87.1% .Conclusions The high frequency ultrasonogram of malignant tumor in the parotid gland were similar and specific,adding the CDFI,PSV,RI and lymphonodus examination around the parotid gland may help to improve the diagnostic accuracy.

Objective To explore the correlation of pulmonary heart disease (PHD) with erythrocyte immunity function and serum erythropoietin(EPO)level. Methods Forty-eight patients with PHD were selected as PHD group, while forty people were chosen as control group. The erythrocyte C3b receptor (E-C3bRR), erythrocyte immunity complex (RBC-ICR) and serum EPO content were detected by yeast rosette method and enzyme-linked immunosorbent assay (ELISA),and blood gas indexes were examined by blood gas analyser.Results The E-C3bRR and serum EPO content were lower, while the RBC-ICR was higher in PHD group than in control group(both P< 0. 01). Compared with control group, PCOz and HCO3-levels were higher, but blood oxygen saturation(SaO2) level was lower in the PHD group than in control group(both P<0. 01). There were no differences in pH value and PO2between two groups(both P>0. 05). The E-C3bRR was positively related to serum EPO content (r=0. 623, P<0.01), and HCO3-was positively related to pH value and PCO2(r=0. 219 ,P<0. 05;r=0. 585,P<0. 01) ,whereas PCO2was negatively related to pH value(r=-0. 529,P<0.01),and PO2was positively related to SaO2(r=0. 682,P<0.01)in PHD group. Conclusions There is a correlation between E-C3bRR and serum EPO content in PHD patients.

OBJECTIVE:To prepare Carbinoxamine maleate sustained-release suspension,and evaluate its quality. METH-ODS:Using carbinoxamine maleate as raw material,drug-loaded resin was prepared by cation exchange resin;surface coating method was used to finally prepare sustained-release suspension,using Eudragit RS100 as sustained-release coating material to pre-pare sustained-release microparticles. HPLC was conducted to determine the content of carbinoxamine maleate,release degree of original preparations and self-made suspensions was compared,drug-loading capacity was calculated. RESULTS:The drug amount in preparing drug-loaded resin was 2%,reaction temperature was 25 ℃,and reaction time was 4 h;the drug-loading capacity in surface coating was 35%,amount of coating material was 10%,and reaction temperature was 40 ℃. The drug-loading capacities of sustained particles before and after coating were 35.23%,32.72%,respectively;the yield was 96.82%. The carbinoxamine ma-leate in prepared sustained-release suspension accounted for 98.76% of the labeled amount;release degree in 10 h reached about 80%,f2 was 65.73. CONCLUSIONS:Carbinoxamine maleate sustained-release suspension is prepared successfully,and its release is similar to the original preparation.

After a literature review of the HPMC capsules, the research status of the HPMC capsules in vivo and in vitro are summarized, including the application status, the superiority over hard gelatin capsules and in particularly the disintegration release in vitro and bioavailability in vivo, as well as pharmacokinetics difference compared with conventional gelatine capsules, are explored in depth. Finally, the future applications of HPMC capsules are prospected.