Objective To investigate the expression and localization of S100B protein in the placenta and umbilical cord tissue of the pregnant women at different gestational ages. Methods The placenta and umbilical cord tissues were obtained from 60 healthy pregnant women at different gestational ages:13～27 weeks (a = 20),28～36 weeks (n = 20),37～41 weeks (n = 20). The localization and expres-sion of S100B protein was detected by S-P immunochemical method and Western blotting. Results In the placenta,the S100B protein lo-cated in the trophoblast.myofibroblasts.macrophages and smooth muscle cells. The intensity of immunostaining and protein concentration in-creased with advancing gestation. In the umbilical cord,the S100B protein was found in the smooth muscle cells,myofibmblasts,amnion ep-ithelium, macrophages and monocytes. S100B piotein expression showed no significant difference in the different cells of the umbilical cord. Conclusion S100B protein expression in the placenta and umbilical cord tissues is throughout the gestation and increases with advancing gestation in the placenta.

Objective To explore the repair effect of methylcobalamin treatment of nerve after spinal cord injury.Methods 108 cases of patients with acute spinal cord injury were selected treated in our hospital from 2010 April to 2014 April , including 60 cases of male, female 48 cases, were randomly divided into Mecobalamin group(n=56) and control group(n=52), mecobalamin group was treat with a cobalt amine, control group was given conventional treatment (dexamethasone intravenous drip note).According to American spinal cord injury score (ASIA) to set sensory function, motor function and spinal cord function score criteria, observed and record ed the sensory function, motor function, recovery of neurological function and complications the two groups of patients were treated for 6 weeks and followed up for 6 months.Results Spinal cord injury patients after 6 weeks and 6 months follow-up, the improvement of sensory and motor function of mecobalamin group were significantly better than the control group ( P <0.01 );Mecobalamin group patients after first to 3 weeks of recovery of neurological function was higher than those of the control group (P<0.05);the 2 groups were found urinary tract infections, lung infections, vomiting, reaction of ulcer complications, but the difference was not statistically significant ( P>0.05).Conclusion In patients with acute spinal cord injury treated by mecobalamin treatment can improve the nerve function damage condition, improve the prognosis and quality of life.

This paper introduces a microtiter-plate scanning instrument for medical laboratory equipment,which is controlled by the embedded PC when moving along the X direction and Y direction on the microtiter-plate plane. The designs of the driving circuit and orienting circuit are included in this paper. This instrument has been used in PKU fluorescence laboratory equipment for the newborn baby.

Artery pulse theories are closely tied to body circulation theories.This design aims to build up a bridge between Chinese medicine and Western medicine through the method of engineering.It uses microscope to enlarge the simulated artery and get the blood pulse curve in an image processing way.In the same condition,there are quite close relations between blood pulse curve and artery pulse curve.It's easy to simulate the changes of the viscoelasticity through changing the artery materials,which can bring up a new artery pulse curve.The differences between the new artery pulse curve and the former one are comprehensively analyzed.This design explains the differences in Windkessel theory,and makes a conclusion.

OBJECTIVE:To explore the level of thyroid hormone in aged female patients with chronic hepatitis C before and after treated with interferon. METHODS:The level of thyroid hormone was detected in 13 aged female patients with chronic hepatitis C treated with interferon at different time points. 15 aged male patients,26 young and middle-aged patients suffering from chronic hepatitis C and 20 healthy subjects were used as control groups. RESULTS:As compared with control groups,TSH was significantly increased,T3,T4,FT3,FT4 in aged female group were significantly decreased after 12 weeks of treatment(P

Objective To understand the situation of nosocomial infections, the distribution of important pathogens of affected patients and the uses of antibacterial drugs.Methods The cross-sectional survey was ap-plied.Before the survey, the investigators were trained by the professionals of the department of hospital infection management.Medical records were reviewed one by one, checked with bedside physical examination.Results In this survey, 283 inpatients were investigated, with an actual check rate of 100%.Two inpatients were affected with nosocomial infection with the prevalence rate of 0.71%.There were two cases of infections (0.71%).Conclusion Investigation of the prevalence rate of nosocomial infection was an important means to monitor the hospital infection, which can monitor specifically, minimize the incidence of nosocomial infection and ensure medical safety by obtaining the information of the departments with high-risk nosocomial infection, the main infectious sites and pathogens.

Objective To compare the clinical application of phloroglucinol combined with oxybuprocaine hydrochloride gel and propofol in artificial abortion .MethodFouhundredcaseundergoing artificial abortion in ouhospital from January 2011 to Decembe2012 were randomly divided into the phloroglucinol group and the propofol group ,200 casein each group .The phloro-glucinol group waintramuscularly injected by phloroglucinol 80 .00 mg a30 .00 min before abortion ,and oxybuprocaine hydro-chloride gel 30 .00 mg by cervical injection a4 .00 min before operation .The propofol group waperformed the operation undethe general anesthesiof propofol combined with fentanyl .The cervical relaxation ,operative time ,pain degree ,intraoperative vaginal bleeding ,vaginal bleeding apostoperative 30 .00 min and occurrence rate of abortion syndrome were observed and recorded in the 2 group.ResultThe operative time ,analgesieffec,occurrence rate of abortion syndrome and operation coshad no statistically significandifferencebetween the two groups(P＞0 .05);buthe cervical relaxation ,dug,monitoring cosand occurrence of respi-ration suppression had statistically significandifferencebetween the two group(P＜0 .01) .Conclusion Phloroglucinol combined with oxybuprocaine can achieve the analgesieffecclose to thaof proprofol in artificial abortion ,ieconomiand practical withouneeding anesthesimonitoring ,and can be used in the primary hospital especially the hospitalwith lack of monitoring devices.

Objective To investigate the ultrasonic features and differential diagnosis of superficial neurilemmoma ,and improve diagnostic accuracy of superficial neurilemmoma by ultrasound .Methods Retrospectively analyse the ultrasonic findings of 51 ca-ses ,which confirmed for superficial neurilemmoma by pathology ,and analyse the misdiagnosis reason from the aspects of size , shape ,boundary ,internal echo ,blood flow signals .Results Superficial neurilemmoma had some characteristics in the shape ,bound-ary ,internal echo ,blood flow signals and so on .However ,the key point to differentiate it from other diseases was to identify the re-lationship with peripheral nerve .In this study ,the coincidence rate of ultrasonoic diagnosis with pathologic diagnosis was 78 .4% . Conclusion Superficial neurilemmoma have some similarity with neurofibroma or sebaceous cyst on ultrasonographic images .When making diagnosis ,especial attention should be paid to identify the relationship of neurilemmoma with peripheral nerve .

Objective To develop an apparatus to measure pulse magnetic field. Methods Single chip computer and Hall-effect integrated circuit was utilized to measure the magnetic induction. The correlative hardware and software flow were given. The measuring range of apparatus was 0-50mT. Results The circuit was simple and the performance was safe and credible. The measuring precision was high. Conclusion It can be used for some kinds of magnetic induction measurement.

Objective To identify the correlation factors associated with cerebral intragenic ischemia after temporary parent arterial occlusion in intracranial aneurysm surgery. Methods One hundred and eighteen patients who underwent temporary arterial occlusion in the 120 aneurysms (from a group of 324 consecutive aneurysm patients treated from 1996 to 2002) were reviewed retrospectively. These variables included sex, age, presence of preoperative subarachnoid hemorrhage (SAH), neurological clinical grade, operational timing, duration of arterial occlusion, numbers of temporary occlusion, mode of arterial occlusion, intraoperative aneurysm rupture, hypertension, the location of temporary occlusion, aneurysm size, hyperglycemia, atheromatous mass. Univariate and multivariate were used to investigate the relationship between the variates and postoperative ischemic changes. Results The total times of temporary occlusion were 156, with an average of 1.30. The duration of arterial occlusion ranged from 1 to 45 min (9.75?7.75). Seventeen patients (14.4%) demonstrated evidence of new infarction in the vascular territory subjected to temporary arterial occlusion. Conclusion In the univariate analysis, age, presence of preoperative SAH, duration of arterial occlusion, atheromatous mass are all significantly correlated with postoperative ischemic injuries. Multivariate logistic regression revealed that the age, older more than 60 (P= 0.010 3 , relative risk=4.335), and the duration of arterial occlusion, lasting more than 20 min (P= 0.032 9 , relative risk=4.177), have significant correlation with the injuries. Based on these findings, temporary occlusion is safe and useful in aneurysm surgery and the postoperative cerebral ischemia is less likely to occur when the duration of clipping is shorter than 20 min.