The human Dab2 gene has a variety of physiological functions,and plays important roles in various signal transduction pathways.Researches have shown that reduced expression of Dab2 was closely related to the occurrence and development in many malignant cancers,and Dab2 has been regarded as a tumor suppressor.This paper reviewed about multiple researches on the structure and functions of Dab2,and explored the specific mechanisms in oncogenesis.

Objective: To evaluate the therapeutic effect of combining use of acupuncture and drugs on rheumatoid arthritis(RA) in the early and the active stages of by the imaging of bone and joint with 99m TC-methlene diphosphate(MDP). Methods: 102 cases of RA in the early and the active stages were randomly allocated into the compound Fengshining group (Group A),acupuncture group(Group B), and compound Fengshining plus acupuncture group(Group C).They were treated for 30 days. All patients were examined before treatment and after treatment by 99m TC-MDP imaging of the whole body, hands and feet, and analyzing method of random interested region was used to count the ratio of radioactivity in the affected area of RA to radioactivity in adjacent normal bone(T/TN). Meanwhile, joint swelling index, joint tenderness index, morning stiffness, gripping force, and erythrocyte sedimentation rate (ESR) were also observed. Results: T/TN of the Group A after treatments had no significant difference comparing with that before treatment(t = 1.35, P＞0.05), while the Group B and C had significant differences(t = 5.31, t=8.97, P＜0.01). The changes of clinical and experimental examination in the Group C had more significant differences than those in the Group A and B(P＜0.05).Conclusion: Acupuncture therapy could promote RA recovery, and combining use of acupuncture and compound Fengshining had better effect.

Objective To investigate the effect of sulfuraphane (SFN) on proliferation of glioma stem cell line SWO-38, and its mechanism threreof. Methods Cell proliferation of SWO-38 treated with SFN was measured by cell prolifera?tion assay. Clone formation experiment, tumor sphere formation experiment and Western blotting method were applied to de?tect the ability of cell clone formation and tumor sphere formation, and the expression of stemness relative genes, such asβ-catenin, Oct4, Sox-2 and c-Myc. The effects of SFN and/or miR-124 inhibitor (miR-124i) on the expression of stemness rel?ative genes were compared. Changes of miRNAs (miRNA-9, 21, 221, 124, 128 and 181) induced by SFN were measured by real time quantity PCR. Results SFN suppressed the proliferation of SWO-38 cells in a dose-dependent manner, in which IC50 was (26.41±2.13)μmol/L. SFN also decreased the ability of forming cell clone and tumor sphere, as well as the expres?sion of stemness relative genes (β-catenin, Oct4, Sox-2 and c-Myc) in a dose-dependent manner. At the same time, SFN led to the change in many miRNAs, during which SFN increased the transcription of miR-124 (-5.9-fold) and miR-128 (-2.6-fold), and decreased the transcription of miR-9, miR-21 and miR-221. Compared to the blank control, the expression levels ofβ-catenin, Oct4, and Sox-2 were significantly increased in miR-124i group. On the other hand, the expressions of above genes were also higher in combined group, which was treated with miR-124i and SFN than those in SFN group, but lower than those of miR-124i group. Conclusion SFN can efficiently inhibit the proliferation of giloma stem cells in SWO-38 cell line through miR-124/(β-catenin/Sox-2/Oct4) signaling pathway.

It is of capital importance to establish a reliable experimental animal model of intracranial aneurysm which should be extremely similar to human intracranial aneurysm in histological and hemodynamic aspects.For recent years,the elastase-induced aneurysm model by means of endovascular balloon occlusion has been widely used abroad.This paper aims to review the preparation of animal mode,the advantages and disadvantages of such a model as well as the recent progress in its applications.

Purpose To observe the action time of parthenolide (PTN) as selective nuclear factor-κB(NF-κB) inhibitor in heart of rats.Methods Forty SD rats were randomly divided into control(CON) group,PTN group,dimethyl sulfoxide(DMSO) group,lipopolysaccharide(LPS) group and PTN+LPS group.The first day:PTN group,DMSO group and PTN+LPS group rats were respectively injected interapertoneally PTN(500 μg/kg) and DMSO; other groups rats were injected interapertoneally equivalent saline.The second day(in equivalent to 24 h after injecting PTN):in LPS group and PTN+LPS group LPS(12.5 mg/kg) was administered subcutaneously; other group rats were injected subcutaneously equivalent saline.Every heart was obtained in equivalentce to 1 h after injecting LPS to measure the expression of NF-κB p50 in nucleus,inhibitory kappaB(IκBα) and phosphorylation inhibitory kappaB(p-IκBα) in cytoplasm of cardiac myocytes through Western blotting.Results Compared with CON group,the expression of NF-κB p50,IκBα and p-IκBα had no difference in PTN and DMSO group.And in LPS group and PTN + LPS group,the expression of NF-κB p50 up-regulated significantly(P＜0.05),IκBα down-regulated(P＜0.05) and p-IκBα up-regulated significantly(P＜0.05).Conclusion LPS can activate NF-κB in 1 h and PTN in rats after 24 h has few effect.

The origin of precision medicine was discussed in this article,as well as its advantages and deficiencies at this stage.Tangshen formula was taken as an example to explain a rational and ideal mode of traditional Chinese medical (TCM) precision medicine research under the guidance of TCM theory.In view of the two major problems existing in the TCM modernization research,including the separated strategy among formula-disease-syndrome and independent research approach using genomics,proteomics and metabonomics,critical scientific issues on developing the drug system to biological system research mode and solutions for integrating formula-disease-syndrome were proposed here,and the roadmap and total framework of top design were also provided.The specific methods and the corresponding research results on Tangshen formula at the three stages concluded as formula-disease related,disease-syndrome combinated and formula-disease-syndrome integrated were introduced and summarized.At this time for the innovative breakthrough development of TCM,precision medicine will promote the TCM modernization research,and also will provide a powerful driving force for the establishment of holistic systems medicine.

Objective To assess the difference in detection of simulated chest nodules in different lung fields with dual-energy digital subtraction chest radiography by receive operating characteristic curve (ROC) analysis.Methods Dual-energy digital subtraction chest radiography was performed on 20 volunteers with simulated chest nodules. ROC analysis was made in the evaluation results on regular DR images and soft tissue images in different lung fields respectively.Results Az was greater on the soft tissue images than on the regular DR images in the superior lung fields and outer zone of middle lung fields. There were significant differences in the superior lung fields and outer zone of middle lung fields.Conclusion Dual-energy digital subtraction chest radiography is superior to regular DR image on detecting more chest nodule lesions, especially in the superior lung fields and outer zone of middle lung fields. So the two techniques should be united to diagnose.

OBJECTIVE:To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocar-dial cell of in vitro cultured heart failure model. METHODS:The myocardial cells of neonate rat were grouped into normal control group,model group,positive control group(Deslanoside injection,1×10-7 mol/L),ginsenosides Rg1 group(1×10-8 mol/L),acon-itine group (1 × 10-9 mol/L) or their compatibilities groups (1∶1,2∶1,1∶2,V/V). Except for normal control group,other groups were given 0.8%pentobarbital sodium to induce heart failure model of myocardial cells. After modeling,each group was given rele-vant medicine for 1 h,and then the activities of T-ATPase,Ca2+-Mg2+-ATPase,Na+-K+-ATPase in cells were all detected. The activi-ties of acyl carrier protein(ACP)and lactate dehydrogenase(LDH),and the contents of brain natriuretic party(BNP),TNF-α and total glycogen were measured in cell culture fluid. RESULTS:Compared with normal control group, T-ATPase and Ca2+-Mg2+-ATPase activities were decreased significantly in model group;meanwhile,Na+-K+-ATPase activity was increased signifi-cantly,and ACP,LDH activities and BNP content in cell culture fluid were increased significantly(P<0.05). Compared with mod-el group,the activities of T-ATPase in treatment groups were increased significantly,while the activity of LDH in cell culture fluid was decreased significantly;when the volume ratio of ginsenoside Rg1 to aconitine was 2∶1,protective effect was the strongest;the activities of Na+-K+-ATPase in aconitine group and compatibility groups were all decreased significantly,with statistical signifi-cance(P<0.05). The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups,but the content of to-tal glycogen in cells and the TNF-α content of in cell culture fluid had no change (P>0.05). CONCLUSIONS:Compatibility of ginsenosides Rg1 and aconitine can improve ATPase activities and membranous permeability,regulate BNP secretion and protect myocardial cell of heart failure model,especially the compatibility of ginsenosides Rg1 to aconitine of 2∶1 ratio.

Objective To explore the correlations between Annexin A1 protein expression and clinicopathological character-istics in carcinoma of papillary thyroid.Methods The different expressions of annexin A1 in papillary thyroid tissue and para-cari-noma tissue were investigated by immunohistochemistry.Results Among 69 samples tissues of papillary thyriod carcinoma,the positive rate of annexin A1 was higher than that of 69 para-carcinoma tissues(88.41%vs .8.69%),there was a significant difference (P <0.05).Furthermore,the expression of annexin A1 was correlation with the lymph node metastasis and tumor size,which was higher in ≥1 cm diameter of tumor(P <0.05).Conclusion High AnnexinA1 positive expression in papillary thyroid cancer tissues is associated with tumor malignant progression,which might be a valuable predictor and potential target for the diagnosis and treat-ment of papillary thyroid carcinoma.

Major breakthrough and challenge that encountered by modern western medicine were described in this article. Some standpoints about the necessity of innovative revolution for the development of traditional Chinese medicine, together with the key points should be bring to the attention during ideological emancipation have been put forward here. Chinese scientists agreed that it is the most appropriate timing for us to establish a new medicine system, which we named Holistic Systems Medicine (HSM). The concept and research contents of Holistic Systems Medicine were also introduced in this article. Finally, a proposal for setting up“China Holistic Systems Medicine development project” was proposed.