OBJECTIVE:To observe the efficacy of Ruzengxiao in the prevention and cure of hyperplasia of mammary glands in rats.METHODS:To investigate the therapeutic effect of drugs,the spayed rats were injected i.m with benzoic acid E2 0.8mg? kg-1 qd alt for 90 consecutive days,during the period between 20 and 30 days after modeling,these rats were injected with progesterone 4mg? kg-1 qd alt for 5 consecutive times;30d after modeling,the model animals with hyperplasia of mammary glands were randomized to groups to be treated intragastrically with 0.05% CMC-Na,Ruzengxiao or Tomoxi fen qd for 60d consecutively.Samples were taken at 24h after the last time of drug administration for determination of indexes.RESULTS:After treating the model animals for 2months,the following experimental results were obtained:for the spayed animals,after injection with estradiol(E2)for 3mo,their index of thymus decreased,indexes of uterus and adrenal glands increased significantly;levels of estrogen receptor(ER)in mammary tissues and serum E2 increased significantly,while the serum progesterone(P)deceased significantly,ratio of E2/testosterone(T)increased significantly;the numbers of lobules of mammary gland increased,hyperplasia of interstitial tissues appeared,secretion of acini increased,and the acini were highly distended,level of prolactin(PRL)increased,while levels of luteinizing hormone(LH)and follicle stimulating hormone(FSH)decreased,but no statistic difference was noted as compared with castration group(spayed animal without administration with E2 and P etc.);the E2-induced increase in indexes of uterus and adrenal gland was significantly inhibited after treatment with Tamoxifen or Ruzengxiao 20g? kg-1 for 2mo;Both Ruzengxiao(at different dosage)and Tomoxifen could significantly increase serum Testosterone level but decrease ratio of E2/T in rats with hyperplasia of mammary glands,showing significant differences as compared with model group.All drugs showed no obvious effect on serum levels of LH,FSH,PRL,P and E2 etc in rats with hyperplasia of mammary glands.CONCLUSION:The above results reveal that Ruzengxiao has satisfactory preventive and curative effects on model rats with hyperplasia of mammary glands.This mechanism might be related to the reduction of serum levels of E2,P and PRL,and the ratio of E2/T.

Objective To explore the clinical efficacy of back acupoint pricking and cupping combined with intense pulsed light in the treatment of acne vulgaris.MethodsTotally 160 cases of acne vulgaris treated in our hospital from July 2014 to August 2015 were selected and randomly divided into observation group and control group, 80 cases in each group. Patients in both groups were treated with intense pulsed light, every two weeks, for 4 times. Patients in the observation group were treated with back acupoint pricking and cupping based on the treatment above, every other day, five times as a treatment session, for two sessions. The treatment efficacy was compared between the two groups.ResultsThe total effective rate in the observation group was 96.25% (77/80), which was significantly higher than 77.50% (62/80) in the control group, with statistical significance (P<0.01). Serum T of the two groups after the treatment decreased significantly when compared with those before the treatment. Serum T in the observation group in patients of the same sex decreased significantly after the treatment compared with those in the control group (P<0.01). Total scores of self-perception, emotion function and Qol-Acne score after treatment in observation group were significantly higher than those in the control group (P<0.01). After 3 months of treatment, the recurrence rate of the effective patients in the observation group was 2.60% (2/77), lower than the control group, which was 16.13% (10/62) with significant difference (P<0.01).Conclusion The clinical efficacy of back acupoints pricking and cupping combined with intense pulsed light in the treatment of acne vulgaris is confirmative, which can effectively reduce androgen levels and improve the life quality of patients, with low recurrence rate.

Objective To study the expressions of Toll-like receptor (TLR)2 and TLR4 in colonic mucosa of patients with irritable bowel syndrome (IBS) and in normal subjects. Methods Thirty patients with diarrhea predominant IBS and 12 healthy volunteers were enrolled. The expressions of TLR2 and TLR4 in colonic mucosa were examined by immunohistochemistry (IHC).TLR2 and TLR4 were semi-quantitative analyzed with average absorbence. Results Contrast to healthy controls, the lamina propria of IBS patients showed edema and looseness with lots of inflammatory cells infiltration. There was no difference in expression of TLR2 between healthy controls and IBS patients (P>0.05). Compared with healthy controls, TLR2 in crypt epithelium and TLR4 in luminal surface of IBS patients were significantly up-regulated (TLR2 : 6.7 % vs. 50.0 %,TLR4: 40.0% vs. 0, P<0. 05). The TLR4 expressed in intestine epithelial cell of both the apical surface and the basolateral surface in 86.7% of patients with IBS, and in 50% of healthy controls.The positive cells of TLR4 in lamina propria were higher in patients with IBS than those in healthy controls (70. 084 ± 21. 887 vs. 20. 577 ± 4. 546, P<0.01). The A values of TLR2 and TLR4 in colonic mucosa of the patients with IBS were higher than those in healthy controls (TLR2:0. 3079±0. 0283vs. 0.3886±0. 0510,TLR4:0. 3044±0. 0481 vs. 0. 3971 ±:010996,P<0. 01). Conclusions Inflammatory cells infiltrated into colonic mueosa in patients with IBS suggested that inflammation might participate in the pathogenesis of IBS. Up-regulated expressions of TLR2 and TLR4 in IBS patients supposed that they might contribute to the occurrence of IBS.

OBJECTIVE: To select the proper experimental animal model for research on prevention and treatment of hyperlipidemia. METHOD: Hyperlipidemia models of mouse, rat, golden hamster, guinea pig, rabbit, pigeon and quail often used in the last ten years were compared. RESULTS: Golden hamster and guinea pig models are similar to human beings in lipid metabolism and have unique superiority in experimental study, while the models of rat, mouse, pigeon and quail have significant difference as compared with human beings. Rabbit is one of the best animal models in studying new drugs for regulating lipid and treating arteriosclerosis. CONCLUSION: Factors such as the required time, indexes, the duration of making models, and the cost and feeding of animals should be considered in selecting animal models according with the experimental requirements.

Objective To study the expressions of TLR4, CD14, MD-2 and NF-kB in colonic mucosa in patients with diarrhea-irritable bowel syndrome (IBS-D) , and compared with normal subjects. The purpose of this study is to explore the role of TLR4 and TLR4 signal transduction pathway in the pathogenesis of IBS-D. Methods The expressions of TLR4, CD14, MD-2 and NF-kB in colon mucosa were examined by immunohistochemistry (IHC) in 30 IBS-D patients and 12 healthy volunteers separately. The average absorbance (A value) of TLR4 was analyzed. The positive expression rates of CD14, MD-2 and NF-kB of colonic mucosa were studied. Results Compared with healthy controls, significant upregulation of TLR4 expression relative to controls was found in colon mucosa of IBS-D. A value of TLR4 in IBS-D was significantly higher (0.3971 ±0.0996 vs 0. 3044 ±0.0481). The positive rate and intensity of NF-kB in IBS-D were significantly higher than those in healthy. The number of positive cells of MD-2 showed significant increase in lamina propria of IBS-D against controls. The percent of CD14 positive was upregulated in lamina propria in IBS-D. The expressions of MD-2 and CD14 in intestine epithelial cell were low or negative. Conclusions There is the activation of the signal transduction pathway of TLR4/NF-kB in the colonic mucosa of patients with IBS-D. Up-regulated expression of TLR4 in IBS patients suppose that it might contribute to occurrence of IBS-D.

OBJECTIVES:To isolate and purify a new peptide named as NV-PP-1,from anti-inflammatory protein extracts of Nidus Vespae and to characterize it.METHODS:It was purified by ammonium sulfate precipitation,DEAE-Sephadex A50 ionexchange chromatography,Sephadex G-50 gel filtration and HPLC，respectively.Then it was characterized by HPLC on Protein-PakTM-60 column,IEF-HPCE(isoelectric focusing-high performance capillary electrophoresis),UV spectrum,amino acid analysis,and the N-terminal analysis with DNS-Cl.RESULT:Both HPLC on Protein-PakTM-60 column and IEF-HPCE showed its homogeneity.Its molecular weight was estimated to be 7.079 kD by HPSEC.UV spectrum indicated its maximum absorption at 274nm.Its PI was determined to be 4.08 according to IEF-HPCE.The terminal analysis showed its N-terminal amino acid was blocked.Amino acid analysis of NV-PP-1 indicated that it was rich in Ser,Glu,Gly.CONCLUSION:NV-PP-1 is the first peptide isolated from the anti-inflammatory protein extracts of Nidus vespae.

Objective] Observation of treatment with traditional Chinese medicine mobile cupping combined with needle medicine rubella clinical curative effect, provide more favorable methods for clinical treatment of rubella means. [Methods] Use of stitch and cans together with professor Lu Xiaozuo Anshen Tongren acupuncture and Chinese medicine allergic to treatment of 128 cases rubella patients, 3 days for one time, needle time, 3 times a week, every time retaining needle 1 hour, 10 times for a period of treatment. Traditional Chinese medicine(TCM) on the basis of professor Lu allergy decoction, a month for a course of treatment. And make the clinical curative effect observation and analysis. [Result] Of 128 cases rubella patients, 56 cases cured, 47 cases were markedly effective, effective 14 cases, ineffective in 11 cases, total effective rate of up to 91.4%. [Conclusion] Rubella curative effect with traditional Chinese medicine mobile cupping method and needle acupuncture medicine is visible.

Objective To observe the influence of individualized health eduction on the metabolic index in patients with type 2 diabetes mellitus and thus explore the effective measures of altering life style of patients.Methods The health education plan was made by nurses specialized in diabetes mellitus in 58 patients with type 2 diabetes mellitus in a manner of one nurse to one patient.Metabolic indexes such as BMI,FPG,2hPG and HbAlc were observed after the execution of the plan.Results BMI,FPG,2hPG and HbAlc were all loweled 6 months and 12 months after the execution of the plan compared with those baseline values of these indexes,and more than 90％ patients could persist in the dietary and physical treatment.Conclusions Individualized health education can alter the metabofic indexes of patients with type 2 diabetes mellitus better.The way of one to one can enhance the effectiveness of the education,make the patients culture good life style by themselves and thus delay the occurrence and development of chronic complications associated with diabetes mellitus.

Objective To construct a simultaneous HPLC-UV dual wavelength spectrometry method for detecting three main contents - harpagide, harpagoside and cinnamic acid in Scrophulariae Radix dispensing particles. Methods Ultimate AQ-C18 column (250 mm×4.6 mm, 5μm) was used, with 1%acetic acid solution and acetonitrile as mobile phase of gradient elution. The flow rate was 1.0 mL/min, detection wavelength was 210 nm at former 13 min and 278 nm after 13 min. The column temperature was 30 ℃. Results The linear range of harpagide, harpagoside and cinnamic acid was 0.066 54-0.665 4 μg (r=1.000 0), 0.024 23-0.242 3 μg (r=0.999 9), and 0.100 28-1.002 8 μg (r=0.999 9), respectively. The average recovery (n=6) was 99.80%±1.22%, 100.31%±1.30% and 100.22%±1.24%, respectively. Conclusion The method is simple, repeatable, stable, and can be used for quality control and standardization of Scrophulariae Radix dispensing particles.