Objective:To investigate the inhibitory effect of Trichinella spiralis polypide protein on H7402 cell line in vitro and to assess its anti-tumor effect against hepatocellular carcinoma(HCC).Methods:The Trichinella spiralis polyp- ide protein was isolated from adult worms and newborn mixture.Trichinella spiralis polypide protein was prepared through homogenization and centrifugation,and its concentrations were determined by UV-visable spectrometers.H7402 cells trea- ted with Trichinella spiralis polypide protein(at 0.035,0.070,0.140 mg/ml)served as treatment group,and normal he- patic cell line HL-7702 treated with Trichinella spiralis polypide protein served as control group.Cell proliferation was de- tected by MTT;the effect of Trichinella spiralis polypide protein on migration and invasion ability of H7402 cell line was assessed by scarification test and invasion test.Cell apoptosis was observed by TUNEL.Apoptosis and cell cycle were ana- lyzed by FCM.Results:Trichinella spiralis polypide protein was successfully prepared.The protein at 0.035,0.070,0. 140 mg/ml inhibited H7402 cells proliferation by(22.40?13.80)%,(29.45?16.80)%,and(39.38?17.80)%, respectively.Apoptosis was observed by TUNEL and FCM,with an apoptosis rate of(39.07?0.90)%;the cell cycle was blocked at S phase.Scarification test and invasion test suggested that Trichinella spiralis polypide protein inhibited the migration and invasion of H7402 cell line,with the inhibition rate being(63.79?13.71)%.Conclusion:Trichinella spiralis polypide protein can inhibit the proliferation,migration,invision of H7402 cell line,and it might be a potential anti-cancer agent.

Medical maintenance,content,quality control of medical equipment and their mutual relationships are discussed.It is to set up the content requirement and controlling way for equipment repairing together with their relationships using the theory and method of modern management science to direct,organize,control and coordinate the repairing work of medical equipment.It is also expected to predict,regulate and check every link of the repairing course so as to establish good quality controlling and management system for optimum repairing effect and economic benefit.This is profound to promote the comprehensive improvement of the quality of medical equipment.

Objective To evaluate the value of three-dimensional surface shaded display (SSD) and volume rendering technique(VRT) in diagnosis of scapular injury. Methods Twenty patients of scapular fractures, who were 15 males and 5 males with an average age of 34 years, were examined with SIEMENS PLUS 4 helical CT. Images were reconstructed in workstation with three-dimensional SSD and VRT, and then analyzed and compared with X-ray and 2D CT images. The regions of interesting consisted of acromion, coracoid process, glenoid cavity, supraspinous fossa, scapular spine, infraspinous fossa, lateral margin, medial margin and inferior angle of scapula. All of imagings were evaluated by two experienced radiologists with double blind method. Results 2D CT findings were clinically or operatively confirmed, and also taken as an evaluative standard to analyze the diagnostic rates of the X-ray, SSD and VRT in this study. The positive rates of diagnosis in plain X-ray and SSD were 94.44% and 97.78%, and the false negative rates was 17.65% and 7.84% respectively; the false positive rate of diagnosis of plain X-ray was 0.76%; the positive rate of diagnosis of VRT was 100%. The difference between plain X-ray, 2D CT, SSD and VRT was not statistically significant for scapular fractures, but the quality of imaging to display the fracture details of 3D CT was the best, while 3D CT might obtain the most objective view of scapular fractures. Eight cases of scapular fractures were examined again post-operatively, the reconstructed images of 3D CT displayed the structure of scapular fractures clearly. VRT could show the contour of scapula and position of the internal fixation, as well as the effect of reduction after operation and the healing status of scapular fractures. Conclusion SSD and VRT of spiral CT are effective methods in diagnosing scapular injury. The images of SSD and VRT of spiral CT may provide exact guidance to preoperative protocol and internal fixation. For complicated scapular fractures, these two techniques could be regarded as imaging techniques of choice. Furthermore, VRT provides more important information to apply the internal fixation devices and assess the result of surgical reduction.

Objective To detect the single nucleotide polymorphism(SNP) of chemokine RANTES promoter of healthy and HIV 1 infected individuals of Han and Uiygur Chinese population, and to evaluate the correlation of their SNPs with HIV 1 infection. Methods Case control study was adopted ,Genotypies of RANTES promoter 403 and 28 from 863 samples were detected by DNA sequencing or by polymerase chain reaction restriction fragment length polymorphism. Results There were two SNPs and six genotypes in Chinese Han and Uiygur. 403 and 28 allelic frequencies were high, but there was no significant difference between the two healthy populations. Strong linkage disequilibrium was observed between the two SNPs. RANTES genotypes were AC/AC, AC/AG, AC/GC, AG/GC, GC/GC, and AG/AG. The haplotypes were GC 62 7% in both rases, AC 28 7% and 30 4%, AG 8 6% and 6 8% in Han and Uiygur, respectively. Compared with AC/AC, odd ratio (OR) of RANTES genotypes AC/AG, AC/GC, AG/GC, and GC/GC was associated with reduced susceptibility to HIV 1 infection. Conclusion Same high RANTES promoter mutation was found in Han and Uiygur populations. Compared with AC/AC, odd ratio (OR) of RANTES genotypes AC/AG, AC/GC, AG/GC 9.9%, and GC/GC might be associated with reduced susceptibility to HIV 1 infection. Further study of the implicated in needed

Objective To evaluate the clinical significance and value of MRI for the diagnosis of the meniscus and ligament injury of the knee joint. Methods 27 patients were put under MRI examinations with 1.5 T unit(Magnetom Vision plus, SIEMENS)and confirmed by surgery or arthroscopy. In all patients, the sagittal and coronal multiplane scan was performed with 4mm section thickness. The posterior cruciate ligament (PCL) angle and PCL curvature index were measured in 8 patients with anterior cruciate ligament (ACL) tears and in 22 patients with ACL normal. The results of measuring were statistically analyzed. Results The accurate diagnosis of MRI was 18 of 24 patients (62.07%) and 24 of 31 cases with meniscus injury (77.42%). On MRI, the signs of meniscus injury were as follows: firstly, the linear high signal intensity inside meniscus was abnormal. Secondly, the size of meniscus diminished and the inner edge was pointless. The third, the triangle configuration of meniscus disappeared.The fourth, the lower or superior margin of meniscus appeared as wavelike configuration. The fifth,the distance from outer side of meniscus to articular capsule increased. Only 5 in 9 patients with ACL tears were exactly diagnosed and the accuracy ratio was 55.56%. There were remarkable statistical differences between the ACL tears group and ACL normal group in the results of (PCL) angle and PCL curvature index(P

A polymer film of vitamin B1 was obtained at a graphite carbon electrode by cyclic voltammetric method in phosphate buffer solution (PBS). Cyclic voltammograms of the film electrode in PBS (pH 5) exhibited one pair redox waves, Epa=350　mV，Epc=325　mV，different from monomer vitamin　B1. The peak currents of poly-VB1 films was proportional to (scan rate)1/2, which showed that the charge transfer was controlled by diffusion in the polymer film. The Poly-VB1 modified electrode showed a good catalytic ability to dopamine and epinephrine. At the modified electrod, the peak potential for oxidation of dopamine occurred at 250 mV and 200 mV for epinephrine, while they were shifted towards the negative direction 100 mV and 300 mV respectively at the bare glassy carbon electrode . And their peak current had a linear relationship to the concentrations in the range of 8.0×10-7～1.0×10-4　mol/L for dopamine and 1.0×10-6～1.0×10-4　mol/L for epinephrine.

Objective To study the effects of Trichinella spiralis (T.spiralis) infection on mice with oxazolone (OXZ)-induced colitis and the possible immunologic mechanism.Methods Female BALB/c mice at age 6-8 weeks were randomly divided into four groups (A to D).Each mouse in groups B and D was infected with T.spiralis strains.Twenty-one days after T.spiralis infection,the mice in groups A and B were treated with 50％ ethanol solution,while those in groups C and D were treated with OXZ to induce the murine model of colitis.Several parameters including survival rate,disease activity index (DAI),macroscopic damage and histological score,myeloperoxidase (MPO) activity and the expression of cytokines (IFN-γ,IL-4 and IL-10) in colonic and splenic tissues at mRNA and protein levels were measured 3 and 7 days after modeling.Results No significant differences in the survival rate,DAI score,macroscopic damage score,histological score,MPO activity were observed between mice from groups C and D (P＞0.05).Pre-exposing the mice to T.spiralis strains neither alleviated the mucosal damages nor aggravated the condition of colitis.Compared with group C,the expression of IFN-γ on the third day and the expression of IFN-γ,IL-4 and IL-10 on the seventh day at mRNA and protein levels in colon and spleen tissues were significantly increased in mice treated with T.spiralis and OXZ (P＜0.05).The expression of IL-10 at transcriptional level in spleen tissues on the third day was higher than that of group C (P＜0.05).The expression of IL-4 and IL-10 at protein level in colon tissues on the third day were significantly up-regulated as compared with those of group C (P＜0.05).Conclusion The severity of OXZ-induced colitis in the murine model was neither alleviated nor aggravated by pre-exposing the mice to T.spiralis strains.High doses of IL-10 not only weakened the regulatory effects on Th2 responses,but also induced the production of proinflammatory cytokines,resulting in a new drift of Th1/Th2 without aggravating Th2 responses.Further investigation on the mechanism of T.spiralis-induced over-expression of IL-10 should be conducted,which might increase the practicability of using T.spiralis strains against OXZ-induced colitis.

Objective To evaluate the role of MR imaging and MR cholangiopancreatography(MRCP) for the diagnosis of hepatic hilar cholangiocarcinoma and assessment of tumor resectability.Methods MR imagings were analyzed retrospectively and compared with surgical result in 20 patients with hepatic hilar cholangiocarcinoma.Results The tumor masses were found in the hilar regions on the MR images for 18 patients. It was found that only dilatation of the intrahepatic biliary tree and thickness of the bile duct wall for the other 2 patients. According to the Bismuth classifying, classification of MR was: typeⅠ (n=3), typeⅡ(n=4), type Ⅲ(n=6),type Ⅳ(n=7). Compared with the operative findings, the accurate ratio of classification was 80%, sensitivity specificity and coincidence was 75%, 75%,80% for the assessment of tumor resectablility by MR. Conclusion MRCP combined with MRI is very useful for diagnosis and classification of the hepatic hilar cholangiocarcinoma and assessment of tumor respectability.

ObjectiveTo study the effect of apoptosis in the p athogenesis of great saphenous varicose(GSV) resulting from primary deep ven ous insufficiency (PDVI). Methods Apoptosis and Bcl-2 expres sion in the segment of first valve sinus of the GSV of PDVI of lower limbs w ere detected by transmission electron microscopy(TEM), agarose gel electrophore sis, TUNEL and immunohistochemistry. ResultsThere were 38 case s of PDVLs in experment group and 5 normal GSV in control group. In experiment group,the apoptosis cells(AC) (6.30? 2.70 )and apoptosis rate (AR)(0.42?2 .12) in the first valve sinus of GSV were significantly higher than those in control group(1.60?0.81,0.21?1.10,respectively)(all P