12 patients with histologically proved wegener's granulomatsis were treated from May 1974 to March 1983.The 3-、 5- and 10- year survival rate S were 83%、64%、50% , respectively. The survival rate was higher in the female. Limited lesions gave a better survival rate than generalized ones.Patients with limited lesions should be treated mainly by radiotherapy alone. The optimal dose was 4000-5500 cGy/4-5.5wk.Patients with generalized lesions should be treated by radiotherapy combined with chemotherapy (CTX 4-8g or CCNU 100mg P.O.), hormone (cortisone 30mg/day),and antibiotics.

OBJECTIVE:To establish an RP-HPLC method for the determination of Callic acid and Caffeine in tea pigment.METHODS:The separation of sample performed on Novapak ODS column(250 mm?4.6 mm,5.4 ?m).The mobile phase consisted of acetic acid-methanol-DMF-H2O(2∶3∶35∶160) with a flow rate of 1.0 mL?min-1 and detection wavelength of 280 nm.RESULTS:The linear ranges of Callic acid and Caffeine were 8.16～81.60 ?g?mL-1 and 2.84～28.40 ?g?mL-1.The average recovery rate of Callic acid was 99.8%(RSD=0.5%,n=6) and that of Caffeine was 99.9%(RSD=0.6%,n=6).CONCLUSION:The method is rapid,sensitive and accurate,and suitable for the quality control of tea pigment.

OBJECTIVE:Suggest to improve the quality standard of toxic traditional Chinese medicines.METHODS:The"content determination"and"limit test"of 72 toxic traditional Chinese medicines recorded in Chinese pharmacopoeia(2005 edition) were briefly introduced.RESULTS:Only 11%of the total 72 toxic traditional Chinese medicines had the items of both"content determination"and"limit test".CONCLUSION:The quality standard of toxic traditional Chinese medicines should be improved to achieve quality control in accordance with the standard of"low toxicity but high effectiveness"so as to ensure safe and effective drug use in the clinic.

OBJECTIVE:To explore the effect of micro-powder technique on the application of traditional Chinese medicine. METHODS:The physical property of the micro-powder and its effect on curative effect, toxicity and pharmaceutical engineering were analyzed. The efficacy of traditional Chinese medicine micro-powder was compared with traditional pieces and decoction. RESULTS:As compared with traditional pieces and decoction, traditional Chinese medicine micro-powder represented many advantages but there were a series of problems such as the change of curative effect and the toxicity in the process of application. CONCLUSION:The micro-powder technology is beneficial exploration to the traditional Chinese medicine dosage-form reform, which should be paid attention and studied.

Objective:To investigate the inhibitory effect of Trichinella spiralis polypide protein on H7402 cell line in vitro and to assess its anti-tumor effect against hepatocellular carcinoma(HCC).Methods:The Trichinella spiralis polyp- ide protein was isolated from adult worms and newborn mixture.Trichinella spiralis polypide protein was prepared through homogenization and centrifugation,and its concentrations were determined by UV-visable spectrometers.H7402 cells trea- ted with Trichinella spiralis polypide protein(at 0.035,0.070,0.140 mg/ml)served as treatment group,and normal he- patic cell line HL-7702 treated with Trichinella spiralis polypide protein served as control group.Cell proliferation was de- tected by MTT;the effect of Trichinella spiralis polypide protein on migration and invasion ability of H7402 cell line was assessed by scarification test and invasion test.Cell apoptosis was observed by TUNEL.Apoptosis and cell cycle were ana- lyzed by FCM.Results:Trichinella spiralis polypide protein was successfully prepared.The protein at 0.035,0.070,0. 140 mg/ml inhibited H7402 cells proliferation by(22.40?13.80)%,(29.45?16.80)%,and(39.38?17.80)%, respectively.Apoptosis was observed by TUNEL and FCM,with an apoptosis rate of(39.07?0.90)%;the cell cycle was blocked at S phase.Scarification test and invasion test suggested that Trichinella spiralis polypide protein inhibited the migration and invasion of H7402 cell line,with the inhibition rate being(63.79?13.71)%.Conclusion:Trichinella spiralis polypide protein can inhibit the proliferation,migration,invision of H7402 cell line,and it might be a potential anti-cancer agent.

Purpose to explore a CT classflication of hepatic echinococcal cysts and its clinical application. Materials and Methods reviewed 85 patients who had underwent CT scan in our hospital from 2008-01～2009-12.The CT classification was made according to WHO classification and the CT appearances were analyzed retrospectively.Results Among the lesions,there were 16 CL type,appeared as a unilocular round cyst without wall.15 CE1 type,appeared as a unilocular cyst with a smooth uniform wall.28 CE2 type,appeared as a fluid collection with septa (honeycomb sign),and 6 CE3 type,CT appeared as a fluid collection with a split wall,10 CE4 type,CT appeared as a fluid collection with a split wall (water-lily sign).36 CE5 type,CT appeared as a calcified mass.Conclusion CT classification according to WHO classification for cystic echinococcosis is using to differentiate it from other liver cystic lesions,such as simple cyst and abcess,and is essential when planning clinical treatment.

The Book of Huo You Xin Shu was written by Zeng Shirong,a famous pediatrics doctor in Yuan Dynasty.Based on his ancestors' experiences,together with his own long-term clinical practices,this book discussed critically on issues of how to nurture children and the treatment based on syndrome differentiation. He promoted the concept of scientific nurture and criticized the problems associated with in bringing up children.He suggested that every aspect of the children's daily life must be taken care of under the guidance of scientific recommendations in order to allow children to growth up healthily.Prophylaxis should go ahead of treatment.He mentioned the importance to carefully investigate in the causes and origins of diseases and to accurately distinguish between the exterior signs and interior causes.For an acute case,symptoms should be dealt with first,while for a chronic one the root cause is treated.He pointed out this principle to remind us a dire consequence may be caused due to misdiagnosis and rash treatment.Before the treatment,we have to differentiate between exterior and interior,cold and heat,deficiency and excess,and follow the principle of simultaneous treatment of both exterior and interior,elimination and reinforcement.Moreover,he paid special attention to the effect of Spleen and Stomach functions in developing pediatrics diseases,emphasizing the importance of regulating them in pediatrics treatment.He was the first person to initiate the concept of "four causes of infantile convulsion(Si Zheng)"and "eight syndromes in infantile convulsion(Ba Hou)".He thought that we ought to investigate the causes of disease,catch the key of pathogenesis,pay great attention to the Qi activity,and the most importantly,regulate Qi activity in treatment.

22 cases of posttraumatic neuroma (TN) were seen in Kunming General Hospital from 1979 to 1985.Clinical and pathologycal study showed that:(1) One of the main causative factors of TN was wound infection.(2)It took at least 6 months to form a TN.Those TN which formed within 6 months belonged to chronic proliferative inflammation.(3)Four Clinical and pathological findings were necessary to diagnosis TN.(4) The term,tumor-like nerve hyperplasia.not only a more accurate term than TN.but also helps avoid misunderstanding.

OBJECTIVE: To provide reference for research & development and application of pill preparation. METHODS: Our understanding on the statement "Pill, Is Sluggish" was come up with, and the pertinetn literature and research results were analyzed and summarized. RESULTS: The statement "Pill, Is Sluggish" is not only the description of the traditional pill's dosage-form characteristic, but also contains the requirement on administration method. It is a relative description. There is also difference in speed of taking effect between different kinds of pills. Its origin contains the factor of dosage as well as the factor of dosage-form; It does not apply to some new type pills. CONCLUSION: The connotation of "Pill, Is Sluggish" is extremely rich, which should be viewed relatively, comprehensively and developmentally so as to continuously enrich and develop the dosage form theory of Chinese materia medica and make pills serve better for the clinic.

OBJECTIVE: To compare the yield of cinnaldehydum from Cinnamomum cassia by TCM decoction machine or stainless steel pot at different decoction time, and to determine the appropriate decoction method of C. cassia. METHODS: The content of cinnaldehydum from C. cassia was determined by HPLC. T-test of data and results was conducted. RESULTS: At different decocting time, the yield of cinnaldehydum from C. cassia by TCM extraction machine was higher than by stainless steel pans (P