Objective To investigate the effect of FK506 on the expression of TNF -? mRNA in isolated liver with reperfusion. Methods After the model of isolated liver with reperfusion was set upp,the expression of TNF-? mRNA was detected with RT-PCR in different preservation time of the isolated liver. Results In FK506-treated group, expression level of TNF-? mRNA was obviously lower(P

Objective To investigate the risk and value of the dissection of bursectomy in T2 gastric cancer. Methods A total of 86 T2 gastric cancer patients were divided into dissection of bursectomy group (46 cases) and non-dissection of bursectomy group (40 cases) according to the random number table method. The age, Borrman type, tumor location, degree of differentiation, vascular tumor thrombus, operation type, lymph nodes metastasis, number of dissected lymph nodes, tumor size, blood loss during operation, operation time and postoperative complications were observed. The patients were followed up for 1 year, and the local recurrence, metastasis and survive were compared between 2 groups. Results There were no statistical differences in age, Borrman type, tumor location, degree of differentiation, vascular tumor thrombus, operation type, lymph nodes metastasis, number of dissected lymph nodes, tumor size, blood loss during operation and postoperative complications (P>0.05). No metastasis was found in anterior layer of transverse colon and pancreatic capsule in 2 groups. The operation time in dissection of bursectomy group was significantly longer than that in non- dissection of bursectomy group: (169.13 ± 37.94) min vs. (147.45 ± 30.27) min, and there was statistical difference (P<0.05). The patients were followed up for 1 year, and there was no local recurrence in 2 groups. There was 1 case with liver metastasis in dissection of bursectomy group, and 1 case with bony metastasis in non- dissection of bursectomy group. There was no death in 2 groups. Conclusions The dissection of bursectomy in T2 gastric cancer could be abandoned, in order to reduce the operation time, blood loss during operation, and postoperative complications.

Objective The objective of this was to investipate study the prognostic and influence factors in patients with transanal local resection for stage T1rectal cancer with the distance from anal margin≤8 cm. Methods A fotal of patients with 180 rectal cancer of stage T1with the distance from anal margin≤8 cm from March 2010 to March 2014 were Retrospective analysed,and there were 90 cases received the local resection of rectal cancer as the observation group and 90 patients with the T1stage who under-went radical resection of rectal cancer as the control group. The postoperative recovery effects were compared between the abservation and control groups. The rates of 3-year overall survival and progression-free survival were recorded. The prognostic influence factors of rectal cancer patients at the stage T1with the distance from the anal margin ≤8 cm after transanal local resection were analyzed. Results The operation time,intraoperative blood loss,postoperative anal exhaust time,postoperative hospitalization stay and postoper-ative complications were significantly lower in the observation group than those in the control group(P<0. 05). There were no signifi-cant difference in overall survival and progression free survival between the two groups(χ2= 0. 896,0. 358;P=0. 344,0. 550). Logis-tic multivariate analysis showed that age,degree of differentiation and cutting edge properties were independent risk factors for the prognosis of patients with rectal cancer who were≤8 cm from the anal margin(P<0. 05). Conclusion Transanal local resection for patients with rectal cancer T1stage from distance to anal margin can achieve similar prognostic benefits as radical surgery,and it can promote early recovery after surgery. Age,tumor differentiation and marginal properties are independent factors,which affected the prognosis of the patients undergoing surgery.

Objective:To establish a HPLC method for determinating 9 components simultaneously in Swertia chirayita. Methods:By useing water Sunfire C18 column (4.6 mm× 250 mm,5 μm); Gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase. Setting the column temperature at 30 ℃, the flow rate at 1.0 ml/min, and the detection wavelength at 254 nm.Results:9 components showed good linear relationship within the injection quality range. The recovery rates of wertiamarin, Gentiopicroside, Angelica glycosides,Mangiferin, Isolysine, Gentianoside, Diol glycoside, 8-hydroxy-1,3,5 trimethoxyketone, and Daisy leaf gentinone were 95.38%, 92.41%, 95.14%, 91.87%, 92.24%, 92.51%, 95.08%, 91.72%, 95.74% ( n=6). Conclusion:The method is simple, efficient, sensitive, accurate, economical and practical, with repeatability and stability. It could provide reference for the quality control and comprehensive utilization of Swertia chirayita.