Objective To investigate the biological effects of EB encoding latent membrane protein 1(LMP1) on Tet-on-LMP1 HNE2 nasopharyngeal carcinoma cell line.Methods Proliferation of Tet-onLMP1 HNE2 cell induced by DOX at the different time points was detected by Western blot assay,DNA content assay,cell growth curves assay,colonogenesis assay in the soft agar and MTT colorimetric method.Results With the increasing LMP1 protein expression,Tet-on-LMP1 HNE2 progressed rapidly from G0/G1 to S phase,cells grew faster(P

Objective To study the MRI features of toxoplasmosis encephalopathy.Methods There were totally 20 cases in the group,all patients accepted the examinations of MRI and immunisation sylum(EIISA) with Gd-DTPA enhanced MRI in 17 cases and repeted MRI in 15 cases.5 cases undengone stero-orientation biopsy under CT guided.Results Most of the focus situated at the juncture of gray and white matter of cerebral hemisphere (8 cases) and around the lateral ventricle (6 cases).The lesion was multiple .The lesions had different degrees of contrast enhancement after enhanced scans (17 cases),among them,the patchy enhancement was most commonly seen (9 cases),secondarily was circular or semicircular enhancemeut (7 cases).The immunological serum examination in all the cases were positive IgM or IgG.The treatment for anti-toxoplasma was in effect for all the cases.Conclusion MRI is of significant value in definiting the involved location,extent,time course of toxoplasmosis encephalopathy,and also evaluating the effect of therapy.

Objective: To investigate the protective effects of matrine combined with glycyrrhizic acid on chronic liver injury induced by carbon tetrachloride, and explore the protective mechanism from the points of energy metabolism and CYP enzyme.Methods: The chronic hepatic injury model of rats was induced by CCl4.The changes of activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were measured to observe the protective effect of the two drugs and their combination.The contents of glutamate dehydrogenase (GLDH) in serum and adenine nucleoside three phosphate (ATP), adenosine diphosphate (ADP) and adenine monophosphate (AMP) in liver tissue were determined to evaluate the regulation effect on hepatic energy metabolism and mitochondrial function.The levels of CYP1A2, CYP2E1 mRNA and protein in liver tissue were detected by real-time PCR and Western Blot to evaluate the two drugs and their combination on the regulation function of liver CYP enzyme.Results: Matrine (72.8 mg×kg-1)and glycyrrhizic acid(43.4 mg·kg-1)could decrease the serum activities of ALT and ALT in chronic hepatic injury model, and the combination (matrine 36.4 mg·kg-1+glycyrrhizic acid 21.7 mg·kg-1) had the most significant protective effect (P＜0.05).Matrine (72.8 mg·kg-1)and glycyrrhizic acid(43.4 mg·kg-1)could decrease GLDH in serum,and restore the content of ATP in liver (P＜0.05).Matrine (72.8 mg·kg-1) had no effect on the expression of CYP1A2 and CYP2E1mRNA, and glycyrrhizin (43.4 mg·kg-1) could inhibit the expression of CYP1A2, CYP2E1mRNA and protein (P＜0.05).Conclusion: Matrine combined with glycyrrhizin has obvious regulation effect on mitochondrial function and liver protective effect in chronic hepatic injury model.

Influenza is an acute infectious disease of the respiratory tract and has posed a serious threat to human health. Vaccination is the most effective way to prevent influenza. The traditional inactivated or attenuated live vaccine based on chicken embryo has a long production cycle. Additionally, the surface antigens of vaccine strains and epidemic strains often do not match due to the continuous antigen drift or shift of influenza virus. As a result, the effectiveness of influenza vaccine is significantly reduced, so there is an urgent need to find a rapid and efficient influenza vaccine production strategy to overcome this limitation. In recent years, with the development of genetic engineering technology and various delivery systems, nucleic acid vaccine has been well developed, especially mRNA vaccine has attracted wide attention because of its simple production process and good safety. Therefore, mRNA-based vaccine immunization strategy provides a new direction for the prevention of influenza. This article reviews the molecular design, delivery system and research in influenza in the development of mRNA vaccine, in order to provide a reference basis for the research and development of influenza mRNA vaccine.

Adult atlantoaxial complex fractures, an acute injury which is rare in clinic, may lead to neurological damage or even death. Their current treatments can be conservative or surgical. The conservative treatment may involve collar bracket, Halo bracket, and sterno-occipital mandibular immobilizer (SOMI) while the surgical treatment mainly involves anterior cervical fixation and posterior cervical fixation. This review expounds on the current literature concerning the treatment of adult atlantoaxial complex fractures so as to provide reference for correct choice of treatment methods for this kind of fractures.

Objective:To evaluate the fluoroscopy-guided percutaneous transhepatic forceps biopsy (PTFB) in the diagnosis of biliary strictures.Methods:Literatures on diagnosis of biliary strictures by PTFB published from January 2001 to August 2022 were obtained by searching the databases of PubMed, Embase, Web of Science, the Cochrane Library, China National Knowledge Infrastructure (CKNI), Wanfang, VIP, and China Biology Medicine disc (CBM). Literature inclusion and exclusion criteria were established and the retrieved literature was screened. The quality of the included literature was evaluated using the quality assessment of diagnostic accuracy studies (QUADAS-2). Statistical analysis was performed by using Meta-disc software and Stata software.Results:Eighteen articles involving 1 935 patients were finally included. The heterogeneity test suggested no threshold effect, but there was non-threshold heterogeneity in sensitivity and negative likelihood ratio due to other reasons, so a random-effects model was used, and a fixed-effects model were used for the remaining outcome indexes used due to low heterogeneity. The results showed that the pooled sensitivity of fluoroscopy-guided PTFB for the diagnosis of biliary strictures was 0.796 (95% CI: 0.776-0.814), the pooled specificity was 1.000 (95% CI: 0.985-1.000), the pooled positive likelihood ratio was 21.532 (95% CI: 11.281-41.098), the pooled negative likelihood ratio was 0.234(95% CI: 0.169-0.323), the pooled diagnostic odds ratio was 94.321(95% CI: 46.744-190.320), and the area under the summary receiver operating characteristic curve was 0.929. The pooled complication rate was 9.2% (95% CI: 6.4%-12.0%). Conclusion:Fluoroscopy-guided PTFB may be a superior diagnostic tool for biliary strictures.

Objective:To explore the clinic effects of the head of second metatarsal bone and flap for bone and skin defect at lateral malleolus in children.Methods:Between July 2009 and May 2018, 4 children with ankle bone and skin and soft tissue defects in the Jizhong Energy Xingtai Mig General Hospital were selected, including 3 boys and 1 girl aged 4-13 years old. All cases were lateral malleolus defect with adjacent skin defect, the range of skin defect was 2.0 cm×2.0 cm -4.0 cm×5.0 cm, and the range of bone defect was 1.0-3.0 cm. The area of the flap was 2.5 cm×2.5 cm-4.5 cm × 5.5 cm, and the length of the second metatarsal head was 1.0-3.0 cm. The donor site was closed directly. After the operation, X-rays was performed to evaluate whether the ankle space and lateral malleolus longitudinal development was synchronized with the uninjured limb. Ankle joint function recovery was evaluated according to the American Orthopaedic Foot and Ankle Society (AOFAS). The outcomes of donor foot range of motion, weight-bearing, and walking were observed.Results:All the composite tissue flap survived with good blood circulation, and all the donor sites healed well. All the child patients were followed up for 0.5-2.0 years. The color and elasticity of the flaps were good, without cicatricial contracture. The patients had no inversion of ankle joint, with satisfying loading and walking function. Imaging evaluation showed that the space between ankle points and the longitudinal development of lateral malleolus were roughly synchronous with the healthy side. Two cases were graded as excellent and 2 as good according to the standard of the AOFAS.The donor's foot had normal flexion and extension function, without instability.Conclusions:The head of second metatarsal bone and flap can repair the epiphysis and soft tissue defect of lateral malleolus in children at one stage, and the reconstructed lateral malleolus can develop with the growth of children. It is a satisfactory method of reconstructing the traumatic defect of lateral malleolus in children.

With the transition from quantity-centric healthcare to value-centric healthcare, patient-reported outcome plays an important role in healthcare. Patient-reported outcome is an effective method for evaluating the outcome of hip replacement patients. It can be used not only in science research, but also in clinical practice and medical care quality assessment. This article reviews the patient-reported outcome measures in hip replacement, so as to provide references for clinical practice and research of clinical medical and nursing staff to accurately select appropriate patient-reported outcome measures in hip replacement.

Objective:To systematically analyze and evaluate the bleeding risk prediction model of patients with venous thromboembolism (VTE) , so as to provide a reference for nursing practice.Methods:PubMed, Web of Science, Embase, Cochrane Library, Wiley Online Library, CINAHL, Scopus, ProQuest, Clinicalkey, Wanfang, China National Knowledge Infrastructure, VIP databases were searched for bleeding risk prediction model of VTE patients. The retrieval time was from the establishment of the databases to August 19, 2021. Two researchers independently screened the literature according to the inclusion and exclusion criteria, then extracted the data and evaluated the quality of the literature.Results:Ten relevant literatures were included in this study, including 4 retrospective studies and 6 prospective studies. The area under the receiver operating characteristic curve of one model was greater than 0.7, and the C-index of four models were greater than 0.7. The prediction model contained many relevant factors, including bleeding history, age, anemia, tumor, antiplatelet therapy, gender, renal insufficiency, etc. In the evaluation of risk of bias and applicability, 10 studies had good applicability, but there were bias mainly due to insufficient number of events in the dependent variable, conversion of continuous variables into dichotomous variables and missing data were not reported. Conclusions:The research on the prediction model of bleeding risk in VTE patients is still in the development stage. In the future, risk prediction models with excellent performance in all aspects and low risk of bias can be developed and verified externally.

Objective? To explore the effect of multi-mode interactive continuous nursing on home rehabilitation and complications of patients after total hip arthroplasty(THA). Methods? From January 2017 to July 2018, patients undergoing THA in Zhejiang Provincial People's Hospital were selected by purposive sampling method. They were randomly divided into two groups according to visit numbers. There were 43 effective cases in the control group and 42 in the observation group. The control group adopted the traditional telephone follow-up mode, while the observation group adopted the multi-mode interactive continuous nursing intervention. The Modified Barthel Index(MBI), Harris Hip Score(HHS) and 6-Minute Walking Tes(t 6MWT) were used to compare the rehabilitation effects of the two groups at discharge, 1, 3 and 6 months after operation, and the complications, unplanned hospital visits and hospitalizations of the two groups were compared. Results? There was no significant difference in each index between the two groups at discharge (P＞ 0.05). The scores of MBI in the observation group were (78.57±7.43), (90.36±7.76), (94.41±7.51) respectively at 1, 3 and 6 months after operation, which were higher than those in the control group [(70.35±7.60), (82.40±7.82), (91.86±8.80)], and there were significant differences between the two groups at 1 and 3 months after operation (P＜ 0.05). The scores of HHS in the observation group were (82.05±7.40), (87.52±6.94), (91.17±7.40) respectively, higher than those in the control group (78.40±7.51), (84.09±7.23), (88.37±8.46), there were significant differences between the two groups at 1 and 3 months after operation (P＜ 0.05). The distance of 6MWT in the observation group were (174.76±35.04)m, (241.55±40.67)m, (282.86±46.46) m, higher than those in the control group (151.61±42.52)m, (214.42±48.59)m, (260.35±48.05) m. There were significantly statistical differences in the 1, 3 and 6 months after operation (P＜0.05). In the observation group, there were 1 case of fracture, 9 cases of unplanned hospital visit or hospitalization, 2 cases of dislocation, 1 case of infection and 20 cases of unplanned hospital visit or hospitalization in the control group. Conclusions? Continuous nursing with multi-mode interaction can improve the effect of home rehabilitation after THA, effectively improve the early and long-term hip function of patients, prevent and reduce complications, and improve the quality of life of patients.