Objective We aimed to provide references for nurses allocation by selecting the nursing items and measuring nursing time. Methods We recorded the nursing workload of 130 nurses in 10 wards within one week by literature review, Delphi method and questionnaire investigation. Results Totally 125 items of nursing items were included,101 of which were direct nursing items and 24 of which were indirect ones.The average time per day per patient was 185.1 minutes for level one nursing,95.4 minutes for level two nursing, 58.5 minutes for level three nursing.Average nursing time per day,working time per day per nurse and nursing time per day for per patient in each unit had statistical difference,P<0.05.The time for direct nursing items was 64.13% and for indirect nursing items was 35.87%. Conclusions Allocation of nurses according to measurement of nursing time could reach the aim of reasonable allocation and effective utilization of nursing human resource.

Aim A cerebrospinal fluid (CSF) sample from patient was subjected to microscopy two Angiostrongylus Cantonensis of developing female and male were found and identifed for the first time in the CSF of the patient at the same time. It is the first case in Liaoning province China.

Objective To investigate the ultrasound measured inferior vena cava (IVC) caliber used as an objective indicator to assess water retention of patients with acute heart failure (AHF).Methods A total of 72 consecutive patients with acute heart failure admitted in the emergency department between December 2013 and April 2014 were enrolled.Acute heart failure was defined by the presence of symptoms such as asthmatic embarrassment and nocturnal paroxysmal dyspnea with or without signs of tracheobronchchial rale and edema of lower limbs,and by objective evidence of cardiac dysfunction as well,either a left ventricular ejection fraction (LVEF) ≤ 45％ or the combination of both left atrium dilation (≥ 4 cm diameter in the parasternal long axis) and a plasma concentration of N-terminal pro-brain natriuretic peptide (NT-proBNP) ＞ 450 pg/mL (patients under 50 years old) or ＞ 900 pg/mL (patients over 50 years old and under 75 years old) or ＞ 1800 pg/mL (patients over 75 years old) or ＞ 1200 pg/mL (patients with renal dysfunction,glomerular filtration rate ＜ 60 mL/min).Exclusion criteria were chronic hepatic disease and acute myocardial infarction.Another 22 patients were enrolled as control.Independent t tests were used to compare normally distributed continuous variables between two groups,while nonparametric tests were used to compare non-normally distributed continuous ones,and chi-squared tests were used for categorical variables.The relations between IVC inner diameter and other normally distributed variables were assessed by Pearson correlation coefficients.A 2-sided P value ＜ 0.05 was considered statistically significant.Results The congestion score and IVC inner diameter were significantly higher in patients with AHF (P ＜ 0.05 ; P ＜ 0.01).The IVC inner diameter was correlated with NT-proBNP concentration (r =0.339,P =0.01 3) and congestion score (r =0.431,P =0.002).There was no relation between IVC inner diameter and LVEF (r =-0.241,P =0.102).IVC inner diameter had significantly positive correlations with pulmonary artery pressure and tricuspid regurgitation (r =0.414,P =0.004 ; r =0.359,P =0.015).Creatinine,blood urea nitrogen,and bilirubin were independently associated with increasing IVC inner diameter (r =0.313,P =0.032 ; r =0.379,P =0.009 ; r =0.385,P =0.007),while IVC inner diameter had negative relation with glomerular filtration rate (r =-0.337,P =0.021).Conclusions The IVC inner diameter can be used as a measurable and objective indicator to estimate the magnitude of access water retention in patients with AHF.

Objective To observe the clinical efficacy and adverse reactions of desogestrel ethinylestradiol in the treatment of dysfunctional uterine bleeding ( adolescent Dub ) .Methods 86 cases with adolescent functional bleeding were divided into the treatment group and control group ,43 cases in each group .The control group received conjugated estrogen(Premarin) treatment,the treatment group was given desogestrel ethinylestradiol treatment .The hemostatic effect and drug adverse reaction of the two groups were observed .Results The cure rate of the treatment group was 65.1%,that in the control group was 23.3%,there was significant difference between the two groups (χ2 =15.28,P<0.01).The hemostatic time of the treatment group was (33.24 ±12.85)h,which was shorter than (50.31 ± 16.32)h of the control group (t=5.39,P<0.05).The incidence rate of adverse reactions between the two groups had no significant difference (χ2 =0.49,P>0.05).Conclusion Desogestrel ethinylestradiol in the treatment of ado-lescent functional bleeding has exact curative effect ,it has low incidence of adverse reactions and better acceptance .

Objective: To observe the anemia situation and pregnancy outcome of pregnant women with iron deficiency anemia ( IDA) treated with polysaccharide iron complex. Methods:Totally 102 patients with IDA were randomly divided into the control group (51 cases) and the study group (51 cases). The control group was treated with iron dextran tablets orally, and the study group re-ceived polysaccharide iron complex. The improvement of anemia and the pregnancy outcome were compared, and the adverse reactions were recorded. Results:After the treatment, hemoglobin ( Hb) , serum iron ( SI) , serum ferritin ( SF) and serum transferrin receptor ( sTfR) at 24-week pregnancy and before the delivery, the red cell count ( RBC) and mean corpuscular hemoglobin ( MCH) before the delivery in the study group were all better than those in the control group (P<0. 05). The incidence of adverse reactions in the two groups was similar. Conclusion:Polysaccharide iron complex can improve anemia status of pregnant women with IDA, which is worthy of widely clinical use.

Objective To standardize the detection procedure and improve the detection ability through the capability evaluation of transfusion compatibility tests for blood transfusion departments in Dalian .Methods Both on‐site inspection and external quality assessment(EQA) were used .The items of on‐site inspection consisted of equipments ,reagents ,tests ,operating instructions and re‐cords .EQA included ABO grouping ,Rh(D) grouping ,antibody screening and crossmatching .Results 42 of 62 blood transfusion departments were qualified .Only one was unqualified in on‐site inspection because antibody screening were not carried out .The un‐qualified ratio of second‐class hospitals′ EQA was the highest (42 .3% ) .The coincidence rates of antibody screening and crossmatching were 82 .0% and 77 .4% respectively ,while those of ABO grouping and Rh(D) grouping was 100 .0% .Conclusion Relatively fixed staffing in laboratories and continual training was important for the improvement of transfusion compatibility tests .

OBJECTIVE:To observe the effects of gelodipine on plasma ghrelin level and endothelial function of blood vessels in patients with essential hypertension.METHODS:A total of 77 patients with essential hypertension were randomly divided into trial group(n=45)and control group (n=32).Both groups were given low sodium diet,proper exercise, weight reduction and oral diuretics,while the trial group was assigned to receive felodipine 5mg q.d additionally.The course of treatment was 8wk.Before and after the treatment,plasma levels of ghrelin and nitrous oxide(NO),endothelin(ET-1),and brachial artery flow-mediated dilatation(FMD)function were observed.RESULTS:Plasma levels of ghrelin and NO,and FMD were significantly increased (P0.05).CONCLUSION:Felodipine can increase plasma Ghrelin level meanwhile improving endothelial function of blood vessels in patients with essential hypertension.

Objective:To investigate the effect and mechanism of Bushen Yiqi Prescription on apoptosis of human first trimester trophoblasts.Methods:Isolated from first trimester placental tissues,human trophoblasts cultured in media with 5%, 10%,20%drug-containing serum for a 24,48 or 72h period.Then we measured cell viability by MTT assay,cell DNA content by flow cytomester,cell Caspase-3 enzyme activity by Caspase-3 active assay kit,and,cell Fas,FasL,Bcl-2 and XIAP protein.Results:Absorbency of trophoblast in 490nm wavelength increased greatly after being incubated with drug-containing serum for 24,48,and 72 h(P

Objective To explore the change of opioid peptide content such as ?-endorphin(?-EP), dynorphinA_ 1-13(DynA_ 1-13) in plasma and cerebrospinal fluid(CSF) of patients with cerebral hemorrhage, and the interference effect of Naloxone on them.Methods 60 patients with cerebral hemorrhage were randomly divided into Naloxone-treated group and control group. Conventional treatment was used in both groups, then Naloxone-treated group intravenous drip of Naloxone, each 3.0 mg, once a day, for 14 d. The concentrations of ?-EP, DynA_ 1-13 of the patients were measured with radioimmunoassay (RIA) during the acute stage before treatment and at 7th, 14th day after treatment compared with those of normal group (the normal people and the patients were treated by operations). Changes of ?-EP and DynA_ 1-13 in bleeding part and amount of bleeding were analyzed. The scores of GCS and NDS before and after treatment were examined at the same time.Results (1) The content of ?-EP, DynA_ 1-13 in plasma and CSF of patients with cerebral hemorrhage were obviously higher since acute stage than those of the normal group(all P

Objective:To study the effect of Kidney-replenishing herb on the indoleamine 2,3-dioxygenase(IDO) of decidual macrophages.Methods:RT-PCR was applied to analyze the IDO mRNA transcription of decidue;Western blot was applied to analyze the expression of IDO protein on decidual macrophages;ELISA was applied to determinate the IL-10 and IFN-? concentration in culture supernates of decidual macrophages and lymphocytes; high-performance liquid chromatography was applied to determinate the kynurenine and tryptophan concentration in cell culture supernates, the ratio of kyn/try was used to determinate IDO activity.Results:The decidual IDO mRNA transcription of normal pregnancy is higher than that of inevitable abortion; the inhibitor of IDO make the balance of Th cytokine depart from Th2; the serum made from herbal medicine improve the expression of IDO protein and IDO activity, and resume the balance of Th cytokine.Conclusion:Appropriate IDO mRNA transcription and IDO activity of decidue is necessary for normal pregnancy;Kidney-replenishing herb improve IDO activity to appropriate value.