Objective To investigate the effect of rifamycin combined vacuum-assisted closure for promoting the wound healing of ulcerative lymphoid tuberculosis .Methods 78 cases of ulcerative lymphoid tuberculosis were randomly divided into the observa-tion group and the control group .The observation group was given rifamycin combined vacuum-assisted closure for treating the wound surface ,while the control group was given rifamycin for dressing change .The area of wound surface was measured before treatment and on 0 ,10 ,20 ,25 ,30 d after treatment .Then the shrinkage rate of wound surface area was calculated .Results During intervention period ,the shrinkage rates of wound surface area at the given time points in the observation group were higher than those in the control group ,the difference showing statistical significance (P<0 .01) .The wound surface in the observation group reached the healing standard on 30 d .Conclusion Rifamycin combined with vacuum-assisted closure can significantly promote the wound healing of ulcerative lymphoid tuberculosis .

0.05). According to the logistic regression, the OR of age was 1.128 which showed that when age increase 1 year, the possibility of contracting CP was higher. The OR of genotype was 0.187 and 0.421, which showed that people with XX genotype were also more susceptible than those with Xx and xx genotype, when the other conditions were invariable. Conclusion:Specific relationship is found between the susceptibility of severe CP and the ER gene-XbaI polymorphism. People with XX genotype are most susceptive to CP.

0.05),but the average levels of DNMT1 mRNA of mild and moderate groups were significantly lower than that of the control group(P

Objective To investigate the prevalence of macula choroidal neovascularization(CNV) in high myopia patients and the associated risk factors.Methods Fifty-six outpatients(112 eyes) with high myopia were medically examined from Mar.2008 to Jun.2009.The inspection items,including A scan,B scan,diopter and optical coherence tomography(OCT),were carried out to check whether CNV existed and its growth fashion.The patients' basic status,main symptoms and the pathologic change in macula were recorded.Results The prevalence of CNV in the 56 outpatients(112 eyes) was 14.3%.Gender showed no correlation with CNV(r=-0.015,P=0.879),while age(r=0.629,P=0.000),diopter(r=0.725,P=0.000),axial length(r=0.236,P=0.013),posterior scleral staphyloma(r=0.344,P=0.000),atrophy of choroid and retina in posterior pole(r=0.421,P=0.000),lacquer crack(r=0.421,P=0.000) and Fuchs spot(r=0.519,P=0.000) were significantly correlated with the prevalence of CNV.Conclusions CNV may highly occurred in the patients with high myopia.Posterior scleral staphyloma,atrophy of choroid and retina in posterior pole,lacquer crack,Fuchs spot,age,axial length and diopter seem to be the risk factors for the occurence of CNV.

Objective To investigate the effects of high glucose and insulin on the expression of glucose transporter 4 (GLUT4), Cbl-associated protein (CAP) and cytoskeleton protein F-actin of glomerular mesangial cells (GMCs), in order to explore the function of GLUT4, Cbl-associated protein and F-actin in the pathogenesis and development of diabetic nephropathy (DN). Methods Cultured 1097 rat glomerular mesangial cells were divided into 8 groups: control, 10-9 mol/L insulin, 10-8 mol/L insulin, 10-6 mol/L insulin, high glucose (30 mmol/L), mannitol (25 mmol/L mannitol+5 mmol/L glucose), high glucose plus 10-6 mol/L insulin, high glucose plus 10-9 mol/L insulin. Expression of CAP mRNA and GLUT4 was measured by RT-PCR and immunohistochemistry method. F-actin was stained by rhodamine-pholloidin and the fluorescent intensity was calculated by image analysis system. Results The expression of GLUT4 mRNA and protein, CAP mRNA was found in normal giomerular mesangial cells (control), and there was no significant difference in 10-9 mol/L insulin group. The expression of GLUT4 mRNA (P<0.05) and protein (P<0.01), CAP mRNA (P<0.01) level was decreased in high glucose group compared with that of control group, but there was no significant difference in mannitol group. The expression of GLUT4 and CAP mRNA up-regulated with the increase of concentration of insulin. The expressions of GLUT4 mRNA in 10-8 mol/L insulin and 10-6 mol/L insulin groups were 2.06-fold and 2.66-fold of 10-9 mol/L insulin group, of GLUT4 protein were 1.93-fold and 2.83-fold of control, and of CAP mRNA were 1.91-fold and 2.15-fold of control, respectively. The expressions of GLUT4 mRNA, GLUT4 protein, CAP mRNA in high glucose plus insulin group were 2.15-fold, 2.08-fold, 2.14-fold of high glucose group respectively. High glucose decreased the fluorescent intensity of F-actin to 44.5% (P<0.01). 10-8 mol/L insulin and 10-6 mol/L insulin groups increased to 1.224-fold (P<0.05), 1.296-fold (P<0.01) in a concentration-dependent manner. The spearman correlation coefficient between GLUT4 and F-actin was 0.929 (P=0.001), between GLUT4 mRNA and CAP mRNA was 0.905 (P=0.002). Conclusions (1) A certain expression of GLUT4 mRNA and protein, CAP mRNA from GMC is found in normal glomerular mesangial cells. (2) High glucose can inhibit the expression of GLUT4 and CAP mRNA significantly, and facilitate the depolymerization of F-aetin. (3) Insulin can reverse down-regnlation of GLUT4 and CAP mRNA caused by high glucose. (4) GLUT4, CAP and F-actin are important factors in the development of DN.

Objective; To study estrogen receptor gene Xbal and Pvu Ⅱ polymorphisms in patients with aggressive periodontitis (AgP). Methods; Xbal and Pvu Ⅱ DNA was extracted by Chelex-100 and amplified by PCR from buccal swabs of 48 cases of AgP patients and 60 normal controls. The PCR products were analyzed by polymerase chain reaction linked fragment length polymorphism (PCR-RFLP) assay. Results: There were significant differences of the distribution of Xba I genotype between AgP group and control group, female AgP group and female control group, male AgP group and male control group(P<0.05). There was no difference of Pvu D genotype distribution between patient group and control group (P>0.05). Multivariate Logistic Regression Analysis showed that male group was less susceptible to AgP than female group(OR =0. 352), the older was less susceptible to AgP than the younger(OR =0.950), and the xxXx genotype was less susceptible to AgP than XX genotype [OR(Xx) =0.224, OR(xx) = 0.678). Conclusion: Specific relationship is found between the susceptibility of AgP and the ER gene-Xbal polymorphism. People with XX genotype is more susceptive to AgP than xx, Xx genotypes.

Objective: To explore the prevalence and co-morbidity in depression disorder among children and adolescents in Chengdu City. Methods: Using a one-phase design, 4S8S children and adolescents in grades 1～10 were assessed with the Strength and Difficulty questionnaire (SDQ) and Development and Well-Being Assessment (DAWBA) . A structured interview with verbatim reports was reviewed by clinicians so that information from parents, teachers, and children were combined in a manner that emulated the clinical process. Results: (1) The rate of depressive disorder was 1.2% , and that in girls was much higher than that in boys after 12 years old (2.56% vs. 1.42%) . (2) The rate of depressive disorder increased with age from 0.10% (6～8 years) to 2.59% (≥ 15 years) . (3) The co-morbidity was 40% . The most common comorbidity diagnosis was anxiety disorders (29.1%) and behavior disorders (25.5%) . Conclusion: The prevalence of depressive disorder among children and adolescents in Chengdu is 1.2%. Gender and age may be the related factors. The co-morbidity is common in children and adolescents with depression disorder.

Objective To explore clinical effect of choledocholithotomy by laparoscopy-assisted choledochoelectroscopy and endoscopic retrograde cholangiopancreatography.Methods The clinical data of 126 gallstone and choledocholithiasis patients treated by laparoscopy-assisted choledochoelectroscopy(L group)and endoscopic retrograde cholangiopancreatography(E group)were analyzed retrospectively.In L group,the patients were performed LC and choledocholithotomy by laparoscopy-assisted choledochoelectroscopy directly,but in E group,the treatment procedures were finished by two steps.Firstly,the bile duct stones were carried out by ERCP,days later LC operations were performed.Between the two groups,the hospital days,costs,the rate of residual stone,the rate of bihary infection or panereatitis and the rate of stone recur were compared.Results In the two groups,hospital days were(6.5±0.3)d and(12.5±0.5)d,costs were(1.22±0.17)and(1.98±0.24)ten thousand yuan,the rate of residual stone was 0 and 5.3%,the rate of biliary infection or pancreatitis was 1.4%and 15.8%,and the rate of stone recur was 1.4%and 10.5%respectively.When compared each other the difference was statistically significant(t=2.48,1.96,χ2=4.32,4.90,8.79,all P＜0.05).Conclusion In patients with gallstone and bile duct stones,laparoscopy-assisted choledochoelectroseopy presented more predominances when compared with two steps methods.

Objective To investigate the potential differences in efficacy of anterior and posterior cruciate ligaments(ACL &PCL)reconstruction by using arthroscopy between autologous tendon and tendon allograft.Methods A total of 144 patients with ACL or PCL fracture were assigned into two groups,namely anterior tibial muscle tendons allograft(n =82)and tendons autograft(n =63).The graft was fixed by using the Endobutton and Intrafix systems. The general information,drawer test,Lachman test,IKDC score,Lysholm score and Tegner score were compared between groups before and after surgery.The mean follow -up period was 16 months,ranged from 6 to 24 months. Results Both two groups received significant improvement after surgery and met the requirements of ligament reconstruction.However,those patients received autologous tendon had less complications,better knee stability.There were significant differences in Lachman score,ADT/PDT score,IKDC score[(83.43 ±4.37)points vs.(81.05 ± 4.41)points],Lysholm score [(90.59 ±3.43)points vs.(89.03 ±3.25 )points],and Tegner score [(7.79 ± 0.94)points vs.(7.37 ±0.90)points]between the two groups in 12 -month(χ2 =9.509,9.080,t =3.237,2.770, 2.729,all P <0.05).Conclusion The efficacy of autologous tendon is better than tendon allograft in anterior and posterior cruciate ligaments reconstruction,which should be considered has highest priority in treating patients with anterior or posterior cruciate ligaments fracture.

Objective To obtain the CT image 3 D reconstruction of head and neck region of the first Chinese visible human. Methods The CT data of head and neck region of the first Chinese visible human were processed with Siemens 3 D Virtuoso VA(R2.6) software in SGI Graphics Computer Workstation. The 3 D reconstruction of head and neck surface and skeletal structure were carried out and studied. Results The face and neck were well reconstructed. The nose, lips, ears and chin could be clearly shown. The skeletal structures in the head and neck region were fairly reconstructed and displayed very well and even the fine structures in skull base such as foraman ovale and foramen spinosum could be seen. Many anatomic structures such as pituitary gland, brain stem, cervical marrow, sphenoid sinus, nasopharynx could be displayed in detail on the 3 D reconstruction section. Conclusion This study has realized the CT image 3 D reconstruction and visualization of head and neck region of the first Chinese visible human and provided morphologic data for further studies of 3 D structures, comparative studies of anatomy and imaging of the head and neck.