Objective To analyze the status of family hardiness and its influencing factors in children with refractory nephrotic syndrome (RNS).Methods A cross-sectional study was carried out in the family members of 120 children patients with RNS admitted to our hospital from January 2013 to February 2016.The general information questionnaire,family hardiness scale (FHI),general self-efficacy scale (GSES),self-rating anxiety scale (SAS),self-rating depression scale (SDS) and simplified coping style questionnaire (SCSQ) were used for investigation and evaluation.The status of family hardiness of children patients with RNS was summarized and its influencing factors were analyzed.Results (1)Among the scores of family hardiness in patients of children with RNS,the score of responsibility dimension was the highest,followed by the score of control dimension.The self-efficacy scores of the family members were in the middle level,the scores of anxiety and depression were in mild level,and the score of positive coping was relatively higher.(2)The univariate analysis showed that the treatment time,education level of family members,place of residence,family per capita monthly income and medical payment method were the related factors influencing the family hardiness score (P<0.05);the Pearson correlation analysis showed that self-efficacy score and positive coping score were positively correlated with the family hardiness score (r=0.425,P=0.011;r=0.536,P=0.002),while the anxiety and depression scores were negatively correlated with the family hardiness score (r=-0.581,P=0.001;r=-0.671,P=0.000).The multivariate regression analysis showed that the family per capita monthly income,self-efficacy score,anxiety score,depression score and positive coping were the independent factors influencing the family hardiness.Conclusion The family hardiness of patients of children with RNS is in the middle and upper level.The family income,self-efficacy of family members,positive coping style,anxiety and depression emotions are related factors influencing family hardiness.

To observe local inflammation reaction and vein thrombosis on rabbit ear vein with methotrexate , ceftri-axone sodium and normal saline .With the extension use of drugs , the numbers of inflammation reaction and throm-bosis in each group were increased , and antibiotic and chemotherapy drug group had a higher rate than the normal saline group .There was a statistically significant difference of the inflammation reaction between the three test groups on the 3rd and 7th day (P<0.05), and a statistically significant difference of thrombosis between the three test groups on the 7 th day ( P<0.05 ) .The physical-chemical properties of drugs and use of time were factors in-fluencing inflammation reaction and thrombosis .

ObjectiveTo investigate the diagnosis value of intracardiac echocardiography in cesarean section scar pregnancy. MethodsCavity ultrasound was applied in patients of cesarean scar pregnancy with sonographic features and treatment. ResultsIn 52 patients, ultrasound diagnosed accurately in 45 cases, diagnosis accurate rate was 86.5％ (45/52). Sonogram showed gestational sac simply type in 16 cases, mixed echo-type( or mass-based) of 36cases. In 52 cases of CSP, according to their clinical and sonographic features ,2 cases of uterine scar lined laparotomy and the uterus excision repair;three cases of laparoscopic removal of uterine scar pregnancy lesion repair. 6 patients to methotrexate;five cases of abdominal surgery;36 routine uterine arterial embolization in the ultrasound monitor after curettage. ConclusionUltrasound could apply in cesarean scar pregnancy timely,and accurate diagnosis was made;sonographic features according to the patients,help choose the appropriate clinical treatment,and assess efficacy.

Objective To investigate the differences in eNOS gene expression,activity and its metabolites before and after human bone marrow mesenchymal stem cells (hBMSCs) are induced into vascular endothelial cells.Methods hBMSCs were induced into vascular endothelial cells.The morphological changes of the cells were observed under inverted microscope.Transwell assay was used to detect the cells' migration ability.The protein expression of eNOS was detected by immunofluorescence and Western blot.The activity of eNOS was detected by ELISA and the content of NO in cell culture supernatant was determined by nitrate reduction method.Results Compared with those in the undifferentiated group,the morphological changes of the differentiated cells were obvious.Cell migration ability increased by 238.10％ (73.000±7.002 vs.21.000±4.359,P＜0.05).The expression of eNOS protein increased by 114.72％ (0.423±0.011 vs.0.197±0.079,P＜0.05).The activity of eNOS was enhanced by 157.49％ (4.967±0.073 vs.1.929±±0.103,P＜0.05).The synthesis and release of NO increased by 155.67％ (184.909±1.853 vs.72.323±0.426,P＜0.05).Conclusion After hBMSCs are induced into endothelial cells,the expression of eNOS gene increases,their activities increase,synthesis and release of the metabolite NO increase.It may provide a basis for prevention and treatment of cardiovascular diseases with stem cells.

Objective To study the quality control methods for dragon's blood spraying film agent. Methods The pH value and viscosity of dragon's blood spraying film agent were detected. Drug dispersed homogeneous degree and particle sizes were determined with Nano Particle Size Analyzer and microscope. Content of Loureirin B was measured by Ultra Performance Liquid-Chromtography (UPLC). UPLC was performed on Waters C18 column (2. 1 mm×100 mm,1. 7 μm), the wavelength was 280 nm, the column temperature was 40 ℃ , and the mobile phase was 0. 1% formic acid aqueous solution and acetonitrile, and the flow rate was 0. 8 mL·min-1 . Results The pH value and viscosity of dragon's blood spraying film agent were stable, drug dispersion was homogeneous, and particle size of the drug was tiny. The concentration of Loureirin B had a good linear relationship in the range of 15. 51-77. 54 μg. Conclusion This method can be accurately controlled, has good stability and repeatability, and can fully control quality of dragon's blood spraying film agent.

AIM: To explore the mechanism of nicotine against the apoptosis induced by colchicines in rat cortical neurons.METHODS: Cortical neurons were cultured from newborn Sprague-Dawley(SD) rats(less than 12 h).The rate of apoptosis was measured by Hoechst33258 fluorescence staining in the neurons,and the activity of Akt473 was analyzed by assay kit Akt473.RESULTS: The apoptosis of cortical neurons can be induced by 0.1 ?mol/L colchicine.The phosphorlation of Akt 473 decreased significantly(1/3 times of the control group,P

A CdTe/ZnSe quantum-dot submicrobead ( QBs ) , which exhibited fluorescence intensity approximately 2800-fold stronger than that of single quantum dots, was conjugated with the anti-histidine rich protein( HRP )-Ⅱ mAbs using N-( 3-( Dimethylamino ) propyl )-N'-ethylcarbodiimide hydrochloride ( EDC ) method as fluorescence probe. The goat anti-HRP-Ⅱ polyclonal antibodies and donkey anti-mouse polyclonal antibodies were sprayed onto the nitrocellulose membrane as test line and control line, respectively. The resultant fluorescence probes were introduced to the immunochromatographic strip for the quantitative determination of Plasmodium falciparum. For determination of Plasmodium falciparum in serum, the QBs based immunochromatographic strips exhibited a good dynamic linear range from 5 . 8 Parasite/μL to 8010 Parasite/μL with a limit of detection of 5. 8 Parasite/μL. The detection time of the proposed QBs based immunochromatographic strips for each sample was only 15 min. Moreover, the recovery rates of the intra-and inter-assay ranged from 93. 0% to 111. 9%, and 98. 3% to 115. 1% respectively, while the relative standard deviations ( RSDs) of intra-and inter-assay were below 5%.

Objective To develop a method for content determination of sinensetin,eupatorin,and 3′-hydroxy-5,6,7, 4′-tetramethoxyflavone in Orthosiphon stamineus. Methods The determination was carried out on a Symmetry C18 column (4.6 mm×250 mm,5 μm) by HPLC.The mobile consisted of acetonitrile and water containing 0.05% H3 PO4 in gradient elution. The flow rate was 1 mL?min-1 ,the column temperature was 30 ℃ ,the detected wavelength was set at 365 nm, and the injection volume was 10 μL. Results The peak areas and the sample quantity of the three components presented good linear relationship in the range of 0. 50 - 5. 00 μg for sinensetin,0. 50 - 5. 00 μg for eupatorin, and 0. 05 - 0. 50 μg for 3′-hydroxy-5,6,7,4′-tetramethoxyflavone.The average recoveries were 101.26%,100.28% and 99.66%,respectively. RSD were 1.73%, 0.82% and 1.67%, respectively. Conclusion The method is proved to be simple,accurate and can be used for the quality evaluation of Orthosiphon stamineus.

Objective: To investigate the effects of miR-24 on endothelial nitric oxide synthase (eNOS) gene expression with regulation and endothelial cell proliferation, migration, tube formation in human umbilical vein endothelial cells (HUVECs). Methods: Constructed high expression plasmid of miR-24 and miR-24 antisense sequence were introduced into HUVECs and the cells included in 3 groups: Control group, miR-24 group and miR-24 inhibitor group. HUVEC proliferation was detected by MTT test, migration was measured by Scratching and Transwell methods, tube formation was examined by Matrigel assay; mRNA and protein expressions of eNOS and Sp1were determined by RT-PCR and Western blot analysis respectively. Results:①Compared with Control group, miR-24 group had decreased cell proliferation by 45.45% as (0.36 ± 0.04) vs (0.66 ± 0.08),P<0.05; miR-24 group had lower speed of cell migration, decreased number of cell migration by 74.75% as (30.25±3.78) vs (119.80±10.94),P<0.01 and there was no obvious tube formation.②Compared with Control group, miR-24 group showed reduced eNOS mRNA expression by 46.2% as (0.49±0.02) vs (0.91±0.01),P<0.05, reduced protein expression by 49.07% as (0.55±0.05) vs (1.08±0.05),P<0.05; meanwhile, decreased Sp1 mRNA expression by 44.9% as (0.49±0. 01) vs (0. 89±0.02)P<0.05, decreased protein expression by 54.90% as (0.46±0.02) vs (1.02±0.04),P<0.05. In miR-24 inhibitor group, the above indexes were lower than Control group but higher than miR-24 group, the amount of tube formation and the length of tubes were similar between Control group and miR-24 inhibitor group. Conclusion: MiR-24 may inhibit HUVECs proliferation, migration, tube formation and suppress eNOS expression; Sp1 might be one of the important regulators.

As the foundation of medical university students' comprehensive qualities, humanity qualities arc the basic and the most important qualities that a medical student should have. However, researches indicate that present medical students still have defects in humanity qualities. Therefore, to enhance the education of the humanities, it is necessary to take some measures such as to improve the courses, to infiltrate the spirits of humanity in major education and to launch practical activities to create an atmosphere of humanity so as to achieve this goal .