Objective:To investigate the effect of focal adhesion kinase related non kinase (FRNK) on the activation and migration of hepatic stellate cells (HSCs).Methods:Human liver tissue was divided into healthy control group and fibrosis group from March 2019 to September 2019 in Affiliated Hospital of Guizhou Medical University. C57BL/6 mice were divided into wild type (WT) and FRNK gene knockout type (FRNK -/-) groups. The liver fibrosis model was established with carbon tetrachloride (CCl 4). After that, FRNK gene overexpression (Ad-FRNK) was constructed with adenovirus vector. HE and Masson staining were used to evaluate the pathological changes and fiber deposition of liver tissue. Western blot was used to detect the expression of PY397-FAK and α-SMA protein. Mouse primary HSCs were extracted, and the effect of FRNK on HSCs migration was detected by wound healing, activation of Rac and Rho was detected by Western blot. Results:The expression of PY397-FAK protein in human liver tissue with hepatic fibrosis was significantly higher than that in healthy control group (0.88±0.09 vs. 0.73±0.09). FRNK was significantly lower than that in control group(0.68±0.09 vs. 0.79±0.11). After animal model was set up, the degree of liver fibrosis in FRNK -/-mice (153±13)% was more serious than that in WT (100%) group. The expression of PY397-FAK and α-SMA protein was significantly elevated (2.50±0.23 vs. 0.75±0.09, 1.46±0.20 vs. 0.92±0.10). After FRNK gene was re-expressed (100%), the degree of liver fibrosis was mainly reversed [(74±6)%], and the expression of PY397-FAK and α-SMA was accordingly decreased(0.68±0.11 vs. 1.12±0.19,0.68±0.10 vs. 0.85±0.06). In vitro, FRNK inhibited the migration of HSCs [WT∶FRNK -/-∶Ad-FRNK,(339±49)%∶(580±53)%∶(259±33)%] and the activation of Rac and Rho proteins (Rac: 0.54±0.07 vs. 0.91±0.10 vs. 0.77±0.12,Rho:0.45±0.05 vs. 0.64±0.06 vs. 0.53±0.07), all P<0.01. Conclusions:FRNK can inhibit the activation and migration of HSCs which contributed to liver fibrosis. The potential mechanism is related to down regulation of PY397-FAK and inhibition of Rac and Rho activation.

Objective:To explore the application effect of remote rehabilitation management in postoperative follow-up of liver transplantation patients.Methods:A total of 92 patients who underwent liver transplantation in the First Affiliated Hospital of Zhengzhou University from March 2020 to July 2021 were selected by the convenient sampling method. They were randomly divided into the control group and the observation group, with 46 cases in each group. The control group received routine out-of-hospital follow-up management, while the observation group received remote rehabilitation follow-up management. The follow-up management period was half a year. The treatment compliance and self-care ability of the two groups were compared before and after intervention.Results:After intervention, the scores of each dimension and the total scores of treatment compliance in two groups were higher than those before intervention, and the differences were statistically significant ( P<0.05) . In addition, the scores of each dimension and the total scores of treatment compliance in the observation group were higher than those in the control group after intervention, and the differences were statistically significant ( P<0.05) . After intervention, the scores of each dimension and the total scores of self-care ability in two groups were higher than those before intervention, and the differences were statistically significant ( P<0.05) . The scores of each dimension and the total scores of self-care ability of the observation group after intervention were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Remote rehabilitation management mode can effectively improve the postoperative treatment compliance and self-care ability of liver transplantation recipients.

Visual endotracheal intubation technique was applied in the endotracheal intubation teaching for standardized resident training as a routine teaching appliance.The residents were divided into two groups,the anesthetic speciality group and the non-anesthetic speciality group.According to the different teaching targets,teaching periods and basic abilities,the differentiated teaching Settings were built and the different teaching schemes,evaluation index and teachers were applied for the two groups respectively for fulfilling the advantages of visual laryngoscope.Until now,more than a hundred residents were educated with the endotracheal intubation,and the teaching efficiency and quality were significantly improved,which also reduced the incidence of the complications related to endotracheal intubation.

As the most basic and widely used invasive nursing activity, intravenous therapy plays an extremely important role in clinical treatment, and its theoretical system and practical techniques have developed rapidly. This study carries out a comprehensive comparative analysis of domestic and foreign intravenous therapy concepts, practice standards, specialist nurse training, vascular access tools, infusion-related technologies, and Pharmacy Intravenous Admixture Services. The purpose of this study is to grasp the international development and innovation level in the field of intravenous therapy and the current gap in China, so as to provide a complete reference for the professional construction of intravenous therapy and nursing in China.

Linxian County in Henan Province, Northern China is known as the region with the highest incidence and mortality rate of esophageal cancer worldwide. Since 1959, the Henan medical team has conducted field work on esophageal cancer prevention and treatment in Linxian. Through three generations of effort exerted by oncologists over 65 years of research on esophageal cancer prevention and treatment in Linxian, the incidence rate of esophageal squamous cell carcinoma in this area has dropped by nearly 50%, and the 5-year survival rate has increased to 40%, reaching the international leading level. This article aims to summarize the history, present opportunities and new challenges, and explore the experience of esophageal cancer prevention and treatment in Linxian (referred to as the “Linxian experience”), providing reference and guidance to cancer prevention and treatment research in China.