OBJECTIVE To summarize the clinical features, diagnosis, treatment and prognosis of laryngeal rhabdomyosarcoma.METHODS The clinical data of 5 cases of adult laryngeal rhabdomyosarcoma treated in Beijing Tongren Hospital between 2003 and 2015 were analyzed retrospectively.RESULTS All the 5 cases firstly presented with hoarseness. 1 case was treated by combined therapy (surgery followed by radiotherapy and chemotherapy). 2 patients received surgical treatment combined with chemotherapy. 1 patient received surgical treatment only. Another patient received chemotherapy only. Pathological type: 4 patients were embryonal rhabdomyosarcoma, 1 patient was pleomorphic rhabdomyosarcoma. In the follow-up of a period from 11 months to 8 years, 2 cases were alive without tumor, 3 patients were dead.CONCLUSION Adult laryngeal rhabdomyosarcoma is rare. Surgical resection combined with chemotherapy is the main treatment method, and the prognosis is related to the pathological type and tumor stage.

OBJECTIVE To study the incidence,pathogenesis,diagnosis and treatment of laryngeal cyst after partial laryngectomy.METHODS The clinical data of 228 cases of partial laryngectomy from January 2003 to June 2009 were retrospectively analyzed,and related literature was reviewed.RESULTS Two cases were found to have laryngeal cysts after partial laryngectomy during the post-operative follow-up.One occurred after 20 months,and the other occurred after 19 months.The cyst located on the tongue surface of the epiglottic was removed by CO2 laser under suspended laryngoscope,while the one located at the subglottic area was removed via cervical approach.So far,a total of 9 cases were reported.CONCLUSION Laryngeal cyst after partial laryngectomy is uncommon.That may be the mucosa planted during the operation.It could be misdiagnosed as recurrent laryngeal carcinoma.CT or MRI is helpful for diagnosis.Operation with CO2 laser under suspended laryngoscope or via cervical approach may be chosen.

Objective To explore the clinical features,diagnosis and treatment of ectopic thyroid lesions.Methods Retrospectively reviewed the clinical data of 15 patients with ectopic thyroid tissue from Oct.2002 to Jun 2017 in Beijing Tongren Hospital,Capital Medical University and Beijing Anzhen Hospital,Capital Medical University,and figured out the clinical features,therapy and prognosis.Among the 15 patients,there were 2 males and 13 females.There were 8 cases of lingual ectopic thyroid,3 cases of endotracheal ectopic thyroid,3 cases of thyroglossal duct ectopic thyroid,and 1 case of retropharyngeal ectopic thyroid.Follow-up were conducted during outpatient review or by telephone.Results Twelve patients received surgical treatment and the lesions were pathologically confirmed as ectopic thyroid tissue,including 2 cases of papillary thyroid carcinoma.These patients were followed up for 1 to 6 years.Their main symptoms were alleviated and the malignant patients had no recurrence or metastasis.Three patients with lingual ectopic thyroid selected observation and follow-up without any treatment and no progress was observed.Conclusions The incidence of ectopic thyroid is low and the forms are diverse.The clinician should take the possibility of ectopic thyroid and its associated pathological changes into account to avoid the complete removal of the only functional thyroid tissue without preparation.

OBJECTIVE: To introduction the clinical application and surgical technique of the modified rhinotomy for treatment of skull base neoplasms. METHOD: The diagnosis and surgical outcome of 20 patients with skull base neoplasms were reviewed retrospectively. RESULT: All the cases were involvement with nasal cavity, ethmoidal sinus, sphenoidal sinus and medial wall of orbit. Two cases were involvement with pterygopalatine fossa, infratemporal fossa and intra calvarium separately. All cases were treated by modified rhinotomy. Among that 2 patient were with juvenal angiofibroma treated by combined with maxillary transposition and pterional and zygomatic approach, and 2 cases were with craniofacial approach. No complications were found relating to surgery. 6 patients was recurrent during follow-up among 6 to 60 months. CONCLUSION The modified rhinotomy is a effective and safe surgical approach to treat skull base neoplasms located in nasal cavity, ethmoidal sinus and sphenoidal sinus. Tumor involved with pterygopalatine fossa, inferotemporal fossa and intracranial extension, other surgical approach should be used at the same time.
Adolescent ; Adult ; Female ; Humans ; Male ; Middle Aged ; Neurosurgical Procedures ; methods ; Nose ; surgery ; Retrospective Studies ; Skull Base ; surgery ; Skull Base Neoplasms ; surgery ; Young Adult

OBJECTIVE Construct and validate the EGFR-CART (Epidermal growth factor receptor-chimeric antigen receptor T) cells targeting the Fadu cell line of hypopharyngeal squamous cell carcinoma, and provide the preclinical basis for the application of CART cell technology in hypopharyngeal squamous cell carcinoma. METHODS EGFR-CART cells and CART-control cells were constructed by lentivirus transfection. The expression of EGFR antibody in CART cells was detected by FACS fluorescence detection, and the expression of EGFR in Fadu cell line and HCT-116 cell line was verified. RESULTS EGFR-CART cells and CART-control cells were successfully constructed, the infection efficiency was about 67％, and there was no significant difference between the two groups in the distribution of cell subsets. HCT-116 is selected as the non target cells of EGFR-CART, Fadu as target cell. CONCLUSION In this study, we successfully prepared and validated the EGFR-CART cells targeting the Fadu cell line of hypopharyngeal squamous cell carcinoma, and laid the foundation for the application of CART technique in the treatment of hypopharyngeal carcinoma.

Objective To investigate the effect of Shenxiong glucose injection on platelet reactivity during aspirin treatment in patients with acute ischemic stroke.Methods A total of 263 patients with acute ischemic stroke admitted to 12 hospitals from January 2014 to December 2016 were enrolled prospectively.They were randomly divided into aspirin group and aspirin + Shenxiong glucose injection group.The changes of platelet maximum aggregation rate induced by 4 platelet aggregating agents (arachidonic acid,adenosine diphosphate,collagen and platelet activating factor) were detected before and after the treatment.Results There were no significant differences in the demographic data and baseline clinical characteristics between the aspirin group (n =132) and the Shenxiong glucose injection + aspirin group (n =131).At baseline,the maximum aggregation rate of platelet induced by arachidonic acid and platelet activating factor in Shenxiong glucose injection + aspirin group was significantly higher than that in the aspirin group (all P ＜0.05).On the 6th day after treatment,the maximum aggregation rate of platelets induced by the 4 aggregating agents in the Shenxiong glucose injection + aspirin group was significantly lower than that in the aspirin group (all P ＜ 0.001).Conclusion Shenxiong glucose injection had a significant inhibitory effect on platelet reactivity during aspkin treatment in patients with acute ischemic stroke.

OBJECTIVE To evaluate the survival and functional outcomes of advanced laryngeal squamous cell carcinoma after modified supracricoid partial laryngectomy(SCPL).METHODS We selected 65 laryngeal cancer patients treated with modified supracricoid partial laryngectomy from 2002 to 2015 in our hospital.Among them,62 cases were males,3 cases were females with an age ranged from 35 to 80 years(median age 58 years)There were 26 cases with T2 stage,32 cases with T3 stage and 7 cases with T4 stage.We selected 120 laryngeal cancer patients treated with vertical partial laryngectomy at the same period as control.Then we evaluated the functional outcomes of modified supracricoid partial laryngectomy group compared with vertical partial hemilaryngectomy group.RESULTS The 5-year cumulative survival rate and decannulation rate were 82.3％ and 98.3％ for supracricoid laryngectomy group respectively.Decannulation ratewas 86.1％ for vertical partial hemilaryngectomy group(P＜0.05).However,there was no significant difference between supracricoid partial laryngectomy group and vertical partial laryngectomy group in pronunciation evaluation and abnormal deglutition.CONCLUSION Modified supracricoid partial laryngectomy is a good choice for local advanced laryngeal squamous cell carcinoma.