Objective To investigate the efficacy of low dose thalidomide combined with chemotherapy in treatment of multiple myeloma(MM)and the clinical curative effect of the treatment method.Methods From June 2010 to June 2012,80 patients were diagnosed with multiple myeloma as the research object in Traditional Chinese Medicine Hospital of Haining,randomly divided into control group and observation group,with 40 cases in each group.The control group adopted conventional chemotherapy,the observation group were given low dose thalidomide combined with conventional chemotherapy.The clinical effect and adverse drug reactions were compared. Results The total effective rate of observation group (87.5%)was significantly higher than that of control group(65.0%)(P<0.05).The adverse reactions were 87.5%(35/40)in observation group and 100.0%(40/40)in control group,there was no significant difference between the two groups.The mean progression-free survival(PFS)and overall survival(OS)in observation group(5.5 ±1.2 months;1 1.5 ±2.4 months)were higher than those in control group(3.7 ±0.8 months;8.5 ±1.3 months)(P<0.05 ). Conclusion Low dose thalidomide combined with conventional chemotherapy in the treatment of multiple myeloma can improve clinical effect and decrease recurrence rate,with high safety and less adverse reactions.

Objective:To investigate the association between polymorphisms of estrogen receptor ? (ER?) gene (rs3020444) and perimenopausal syndrome (PS) in patients with liver qi stagnation syndrome (LQSS). Metheds:The ER?T/C genotype in 100 patients of PS of LQSS type (PS-LQSS group),86 patients of PS of non-LSDS type (PS-non-LQSS group) and 100 healthy subjects (control) was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay. Results:Partitions of ?2 method showed that the higher prevalence of ER?-TT genotypes was noted in PS-LQSS group (?2=8.307,P=0.004),and the higher prevalence of T alleles was noted in PS-LQSS group (?2=7.129,P=0.008). and Multinomial logistic-regression indicated that the odds ratios (OR) of the ER?-TT genotypes vs TC/CC for PS-LQSS was 2.222 (95%CI:1.172-4.744,P=0.015) after adjusting for common miscellaneous factors. Conclusions:It was suggested that ER?-TT genotypes was significantly associated with PS-LQSS,and ESR?-TT may be one of the genes that contribute to PS-LQSS.

Objective To study the effect of local mild hypothermia on AKT protein expression after rats focal cerebral ischemia-reperfusion injury.Methods By using the model of rat focal cerebral ischemia-reperfusion injury,the middle cerebral arteries(MCA)of SD rats were occluded for 2 h,and reperfused for2h,12h,24h,72h.Immunohistochemical analysis was used to detect expression of AKT.Results AKT expressed after ischemia 2h,the expression was significantly increased after ischemia reperfusion with peak expression at 72h in model group on normal temperature.In mild hypothermia group,the AKT expression was higher than that in normal temperature group when at the same time point.Conclusion Brain hypoxia-ischemia could induce the expression of AKT.Mild hypothermia could promote the expression of AKT.The expression of AKT could be one of the protected way of brain hypoxia-ischemia.

BACKGROUND: Polyblends composed of thermoplastic polyurethane elastomer (TPU) and bisphenol A type of polysulfone (PSF) or polymethyl methacrylate (PMMA) can improve the properties of TPU, bisphenol A type of polysulfone or PMMA and can widen the scope of application. The property of polyblends bad or good depends on the miscibility of polyblends. The dilute-solution viscometry (DSV) is a simple and rapid way for determining the miscibility for polyblends. The study on the determination of miscibility for TPU/PSF and TPU/PMMA polyblends by the DSV has not been reported up to now. OBJECTIVE: To differentiate the miscibility of TPU/PSF polyblend and that of TPU/PMMA polyblend, and at the same time to verify the consistency of μ and α criteria in the determination of polyblend miscibility by DSV.DESIGN: Observation and contrast analysis based on the two polyblend systems.SETTING: Chemical Engineering and Pharmaceutics College, Henan University of Science and Technology.MATERIALS: TPU sample was purchased from Luoyang Jiming Chemical Industry Limited Company, China. PSF sample was purchased from the First Plastic Factory of Dalian, China. PMMA sample was synthesized by our laboratory. N, N-dimethylformamide was provided by Beijing Chemical Plant.METHODS:This study was performed at the Key Laboratory of Polymer Science and Nano-technology, Henan University of Science and Technology in May 2006. The different molar ratio of TPU / PSF and TPU / PMMA polyblends were prepared in N, N-dimethylformamide. An ubbelohde dilution viscometer (whose inner diameter was between 0.5 mm and 0.6 mm) was employed for measuring the relative viscosities of the polymer solutions in DMF at (25±0.01) ℃. The second stop-clock was used to record efflux time. From the efflux time, the relative viscosities could be obtained. Then the specific viscosities could be given by the relative viscosities value. From a series of relative viscosities, the specific viscosities and other data of two polyblends, μ values and α values of two polyblends were obtained.MAIN OUTCOME MEASURES: The efflux time of two polyblend solutions in an ubbelohde viscometer.RESULTS: The μ and α values of TPU/PSF polyblend were all above zero, which showed that TPU/PSF polyblend was miscible. But the μ and α values of TPU/PMMA polyblend were all below zero, which showed that TPU/PMMA polyblend was immiscible. CONCLUSION:The μ criterion is consistent with the α criterion in judging for polyblend miscibility. DSV method is simple, and it can be used in the determination of polyblend miscibility.

BACKGROUND: Typically antiepileptic drugs, such as phenobarbital, fenitoina sodica and diazepam, can inhibit amygdala kindling effect in rats. However, whether midazolam has the same effect is still unclear.OBJECTIVE: To investigate the inhibitory effect of midazolam on amygdala kindling onset in rats and maximal electroshock seizure (MES) in mice and effeots of antiepileptic drugs.DESIGN: This study was divided into three subexperiments, including the effects of midazolam on amygdala kindling onset, independent activities and incidence convulsion. All the three subexperiments were completely randomized,infra-group control or self-control studies.SETTING: Medical College of Qingdao University.MATERIALS: The experiment was carried out in the Comprehensive Experimental Room, Affiliated Hospital of Medical College of Qingdao University from August 2004 to March 2005. Nine Wistar rats weighing (250±10) g and 120 Kunming mice weighing (20±5) g and either gender were provided by Animal Center of Qingdao Institute of Drug Control.Midazolam (5 g/L) was provided by Xuzhou Enhua Drugs Co., Ltd. (batch number: 20030706).METHODS: ① Establishment of amygdala kindling models: Nine kindled rats were randomly selected and intraperitoneally injected with 0.25, 0.5 and 1 mg/kg midazolam, respectively. Quadri-pathway biological signal processing system (SMUP-PC) was used to measure discharge duration (ADD) and Racine's stage. ② Sixty mice were randomly divided into 5 groups, including saline group, 40 mg/kg phenobarbital group, 0.5, 1.0 and 1.5 mg/kg midazolam groups with 12 mice in each group. And then, numbers of activities in a unit time (times per 5 minutes) were determined by XZC-4A mini-animals independent activity instrument. ③ MES models were established to calculate incidence of convulsion.MATN OUTCOME MEASURES: Effects of midazolam on ADD, Racine's stage, numbers of independent activities and incidence of convulsion.RESULTS: All the 9 rats and the 120 mice were involved in the final analysis. ① Effect of midazolam on amygdala kindling onset: After intraperitoneal injection of 0.5 and 1.0 mg/kg midazolam, ADD and Racine's stage were obviously lower than those before administration (P ＜ 0.05-0.01). After intraperitoneal injection of 0.25 mg/kg midazolam, ADD was obviously lower than that before administration (P ＜ 0.05), but there was no significant difference in Racine's stage. ②Effect of midazolam on independent activities of mice: Numbers of independent activities were lower in the phenobarbital group and 0.5, 1.0 and 1.5 mg/kg midazolam groups than those in the saline group (P ＜ 0.01), while numbers of independent activities were higher in 0.5 mg/kg midazolam group than those in the phenobarbital group (P ＜ 0.05). ③Effect of midazolam on maximal electroshock seizure: Incidence of convulsion was lower in the phenobarbital group and 0.5, 1.0 and 1.5 mg/kg midazolam groups than that in the saline group (P ＜ 0.05-0.01), while Incidence of convulsion was higher in 0.5 mg/kg midazolam group than that in the phenobarbital group (P＜ 0.05).CONCLUSION: Midazolam can significantly inhibit amygdala kindling onset, reduce numbers of independent activities,and antagonist MES in mice.

Objective:To investigate the development of specific IgM and IgG antibody against the novel coronavirus in patients with SARS (severe acute respiratory syndrome).Methods:IgM and IgG antibodies against the SARS virus in the sera of the first week, second week, third week, fourth week, eighth week, twelfth week after onset of the illness in the 20 patients with SARS were detected by the indirect enzyme linked immunosorbent assay.Results:IgM and IgG were found negative in the first week after onset of clinical symptoms in all 20 patients with serial sera. Of these 20 patients, 16 were IgM positive and 17 IgG positive in week 2. All 20 patients were IgG positive since the third week and maintained a high level up to 3 months. However, patients with IgM positive was gradually decreasing from week 3 and none of 20 patients was IgM positive in week 12.Conclusion:Specific IgM antibody were found in most patients with SARS during the acute or early convalescent phase and disappeared by the twelfth week after onset of illness. Positive IgM reflected the recent infection of SARS virus. Serum IgG antibodies persisted for a longer period after infection and might be protective and provide immunity from recurrence of symptomatic disease.

Objective To investigate the effects of atorvastatin on cardiovascular remodeling in renovascular hypertensive rats. Methods The 2-kindey,1-clip hypertensive rats(2K1C,Goldblatt) were prepared with Sprague-Dawley rat.SD rats were randomly divided into three groups: normal control rats,hypertensive rats and hypertensive rats treated with atorvastatin(2 mg?kg~(-1)?d~(-1)).After 6 weeks treatment,systolic blood pressure(SBP) was measured using the tail-cuff method.The plasma concentration of angiotensin Ⅱ and renin activity were determined by radioimmunoassay.The heart weight,the ratio of left ventricular weight to body weight were calculated.Results The plasma concentration of angiotensin Ⅱ((106.4?7.8)ng/L) and renin activity((20.6?2.4) ng/L)were significantly increaed in hypertensive rats compared with normal rats((72.3?5.4) ng/L and(12.5?3.7) ng/L)(P

Objective To investigate the effect of miR-375 inhibited by 2'-O-me-375 on lipoapoptosis of NIT-1 pancreatic β cells. Methods NIT-1 cells were divided and treated according to the optimal condition: mock (without lipofectamine) ,lipofectamine( transfected only with lipofectamine) ,NC-miRNA (transfected with negative control miRNA) ,and 2'-O-me-375( transfected with 2'-O-me-375) groups. 72 hours later, all cells in each group were cultured with 500 μmol/L palmitate for 48 h. The percentage of apoptotic cells was detected by Hochest33342 staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL). The protein expression of myotrophin ( V1 ) , a target gene of miR-375, was detected by Western blotting. Results Compared to the other three groups,the cell apoptosis rate of 2'-O-me-375 group was the lowest (P<0.01) .along with the highest VI expression level(P<0. 01). Conclusion Inhibition of miR-375 decreases pancreatic (3-cell lipoapoptosis.

Objective To study the expression and clinical significance of ARHGAP4 in colorectal cancer Method Real?time PCR,Western blot and immunocytochemistry were used to detect the expression of ARHGAP4 in colorectal cancer tissues and cell lines ,and the correlation between its expression and clinical features of patients was analyzed Results ARHGAP4 is overexpressed in colorectal cancer tissues and cell lines and its overexpression is correlated with T stage, N stage, clinical stage, and metastasis. Conclusion ARHGAP4 may promote the progression of colorectal cancer ,and have the potential to be a novel prognosis marker.

OBJECTIVE:To evaluate the characteristics and regularity of adverse drug reactions (ADR) in children's hospital. METHODS:The clinical drug use and ADR of 3 653 children during 2006～2008 in our hospital were analyzed statistically by designing a child medication registration form. RESULTS:The incidence of 498 ADR cases was about 13.63%; ADR were mostly occurred in 1～3 year-old children(36.14%); 71.29% were caused by intravenously; 66.87% were induced by antibiotics; ADR were more common in spring and winter. CONCLUSION:The drugs have a dual nature. Moreover,children belong to a special group. The measures such as strictly mastering clinical indications,reducing irrational drug use,sufficient therapy and avoiding or reducing the occurrence of ADR can guarantee children grow healthily.