1.Surveys of researches of Chinese herbal medicine that rteats AIDS
Junhui ZHANG ; Xuejian XIE ; Aihua MA ;
Journal of Medical Postgraduates 2003;0(12):-
To summarize the progress of researches of activated components in Chinese herbal medicine and compound preparations that resist human immunodeficiency virus in recent years.Very vast foreground of Chinese herbal medicine in the treatment of AIDS hal been elucidated.
2.Clinical study of combined interventional embolization for the treatment of portal hypertention
Xuejian LIU ; Enshan LI ; Youping MA ; Chunqing ZHANG
Chinese Journal of General Surgery 1993;0(01):-
0.05).(4) Free pressure of portal vein(FPP) significantly declined at post-operation in the two groups(P
3.Effect of ginsenoside Rh_2 on cultured PC-3M cells in vitro
Guihua ZHOU ; Yan MENG ; Yang LI ; Xuejian ZHAO ; Yanping CHEN ; Xingyua MA
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To explore the influence of ginsenoside Rh 2 on cultured PC-3M cell cycle in vitro . METHODS: DNA content was measured using -TdR incorporation assay. To analysis the cycle changes of PC-3M cells, flow cytometry was used in the experiment.RESULTS: The results indicated that the rate of -TdR incorporation was lower in Rh 2-treated cells than the control in a dose-dependent manner. Flow cytometry demonstrated that the pecent of G 1 phase treated with Rh 2 was higher than that of control. CONCLUSION: These results suggested that PC-3M cells cultured with Rh 2 for 24 h were blocked at G 1 phase, and Rh 2 inhibited the growth of PC-3M cells in vitro .
4.Effects of prolonged heavy load swimming and the Panaxadiol saponins on expression of ?-actin of quadriceps in rats
Ke YANG ; Dan ZHAO ; Feng LI ; Zhinong WANG ; Xuejian ZHAO ; Yanping CHEN ; Xingyua MA
Chinese Journal of Pathophysiology 2000;0(08):-
AIM: To study the effects of 7 weeks prolonged haevy load swimming and Panaxadiol Saponins (PDS) on the expression of ?-actin in quadriceps muscle of thigh in rats. METHODS: After 7 weeks prolonged heavy load swimming training, the expression of ?-actin in quadriceps muscle of thigh was quantitatively examined by Northern blotting analysis in the condition with or without PDS treatment. RESULTS: The expression of ?-actin in rat quadriceps muscle of thigh in exercise+PDS group was significantly higher than that in sedentray control group and exercise+saline group at 24 th hour after intensive training. No statistical difference between exercise+androsan group and exercise+saline group was observed.CONCLUSION: PDS enhanced ?-actin expression in quadriceps muscle of thigh in rats.
5.Effect of panaxadiol saponins on fluidity of red blood cell membrane in heavy load swimming trained rats
Ke YANG ; Feng LI ; Zhinong WANG ; Xuejian ZHAO ; Guixiang XU ; Yanping CHEN ; Xingyua MA
Chinese Journal of Pathophysiology 1986;0(02):-
AIM: To study effects of prolonged intensive endurance exercise and Panaxadiol saponins (PDS) on red blood cells (RBC) membrane fluidity in rats, as well as the mechanism. METHODS: By the membrane fluorence polarization, Pyrollol-NBT, TBA methods and Scanning Electron Microscope, RBC membrane fluidity,RBC-SOD and serum MDA were measured after 7 weeks prolonged intensive swimming. RESULTS: Fluorence polarization(P value) were significant higher and fluidity were lower( P
6.Ginsenoside Rh_2 enhances the apoptotic effect of cisplatin on PC-3M prostatic cancer cells
Yan MENG ; Guihua ZHOU ; Yang LI ; Xuejian ZHAO ; Yanping CHEN ; Xingyua MA
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To study the inducing apoptotic effect of Cisplatin (DDP) combined with Ginsenoside Rh 2 on PC-3M prostatic cancer cells in vitro. METHODS: Cell viability was examined by MTT test and the apoptosis of PC-3M by electrophoresis of NP-40 extracting fragmental DNA and flowcytometry. RESULTS: Pc-3M cells were exposed to 15.0 mg/L Rh 2 combined with 0.4 mg/L DDP: ①Cell viabililty inhibitory rate was increased by 1.44 times from 25.0% (with 0.4 mg/L DDP) to 61.03%. ②Characteristic apoptotic DNA laddering was found more obviously than using either agent alone. ③Apoptotic rate of PC-3M was increased from 0.32% (with 0.4 mg/L DDP) to 52.2%, which showed similar effect with 4.0 mg/L DDP, it was much higher than that of 15.0 mg/L Rh 2 alone (13.6%) ( P
7.Autologous bone marrow stem cells implantation to bone inducing active material combined with core decompression in the treatment of early femoral head osteonecrosis
Xiuli WANG ; Yisheng WANG ; Xuejian WU ; Xuan ZHAO ; Yi ZHANG ; Yuan MA ; Ming LI ; Zhi QIAO
Chinese Journal of Microsurgery 2017;40(2):142-145
Objective To observe the curative effect of autologous bone marrow stem cells implantation to bone inducing active material combined with core decompression in the treatment of early femoral head osteonecrosis (FHON).Methods From April,2010 to March,2012,in Department of Orthopaedics,the First Affiliated Hospital of Zhengzhou University,a total of 79 adult patients with 108 hips suffered from the early stage FHON were treated with autologous bone marrow stem cells implantation to bone inducing active material combined with core decompression through the core of the femoral canal,male of 65 cases,female of 14 cases,the mean age was 29.5 (20-50) years old.According to the etiology classification:the alcohol-induced FHON was in 54 patients with 66 hips,steroid-induced FHON in 14 patients with 20 hips,steroid and alcohol-induced ONFH was in 11patients with 22 hips.According to association research circulation osseous (ARCO)classifying,Ⅰ-A,Ⅰ-B,Ⅱ-A,Ⅱ-B phases were 6,16,8,and 78 hips,respectively.There were 43 hips in left side and 65 hips in the right side.Results All patients were followed up from 4 to 6 (4.8 ± 0.6) years.Compared with before operation,the scores of all patients were significantly increased (P < 0.05).All patients with hip pain symptoms were relieved or disappeared.The healing tine of the patients in all age groups was statistically significant (P < 0.05),and with the increase of age,the healing time was prolonged.The excellent and good rates of Ⅰ-A,Ⅰ-B,Ⅱ-Aand Ⅱ-B were 100% (6 / 6),100% (16/16),100% (8/8),and 98.7% (77/78).The X-ray showed that coarse channel osteogenic phenomenon is obvious,there is 1 case collapse of femoral head of stage Ⅱ-B,the rest were not collapse.Conclusion The treatment of early osteonecrosis of the femoral head with autologous bone marrow stem cells implantation to bone inducing active material combined with core decompressionis definitely effective,especially in patients with ARCO:Ⅰ-A,Ⅰ-B and Ⅱ-A phase,and the effect of ARCO:Ⅰ-A and Ⅱ-A is the best.
8.Gut microbiome characteristics in patients with type 2 diabetes mellitus of Dongxiang Group in Gansu province
Xiaolan MA ; Yanjun HOU ; Zhiming MA ; Xuejian HU ; Dan GE
Chinese Journal of Postgraduates of Medicine 2023;46(4):305-310
Objective:To investigate the region-specific characteristics of the gut microbiota and evaluate the association of speci?c gut microbes with type 2 diabetes mellitus (T2DM) from the Dongxiang Group in Gansu province, Northwest China.Methods:Fifty-three participants who was born in Dongxiang Autonomous County (Gansu Province) from April 2020 to January 2021 were enrolled, including 25 patients with T2DM recruited from the outpatient departments of internal medicine at The People′s Hospital of Dongxiang County(T2DM group) and 28 healthy controls recruited from the health screening center (HC group). Gut microbiome composition was analyzed using a 16S ribosomal RNA gene-based sequencing protocol.Results:A total of 936 operational taxonomic units (OTU) were obtained in the two groups. Of note, the HC and T2DM groups had 633 OTU in common. The alpha and beta diversity were different between the two groups ( P<0.05). Shannon index was significantly higher than that in the HC group, and Simpson index was significantly lower than that in the HC group, displacement multivariate analysis of variance was used to compare β diversity between the two groups, and the difference was statistically significant ( P<0.05). At the Phylum level, firmicutes and actinomycetes in T2DM group were significantly higher than those in the HC group (37.97% vs. 22.89%, 5.09% vs. 2.08%), and the differences were statistically significant ( P<0.05). The abundance of Bacteroidetes was significantly decreased (68.00% in T2DM group and 49.75% in HC group), and the difference was statistically significant ( P<0.05). At the genus level, there were 20 genera statistically significant differences between the two groups. The abundance of Bifidobacterium, Escherichia, Shigella, and Tyzzerella_4 levels were significantly increased in the T2DM group, but Prevotella_9, Erysipelotrichaceae_UCG-003, and Roseburia levels were significantly decreased in the T2DM group compared to those in the HC group. Conclusions:There is a significant difference in the gut microbiota between patients with T2DM and healthy individuals of the Dongxiang group in Northwest China. So as to preliminary exploration the intestinal flora characteristics of T2DM in the Dongxiang group.The findings of this study provide a theoretical basis for the prevention and control of T2DM in Dongxiang group in the future.
9.Thoracic vertebroplasty guided by a self-designed sight
Lei YU ; Caoyuan MA ; Yawei LIU ; Zhengyi WANG ; Chaojun XU ; Yingjie HAO ; Xuejian WU
Chinese Journal of Orthopaedic Trauma 2018;20(6):499-503
Objective To investigate the clinical efficacy of our self-designed sight used to guide thoracic vertebroplasty.Methods A retrospective analysis was conducted of the 52 patients (70 thoracic vertebrae) who had undergone percutaneous vertebroplasty (PVP) (n =36)or percataneous kyphoplasty (PKP) (n =34) of T1-T4 vertebral bodies at Department of Orthopaedics,The First Affiliated Hospital to Zhengzhou University form August 2012 to October 2013.The operation time,intraoperative bleeding,intraoperative radiation by C-arm roentgenography,and visual analogue scale (VAS) were compared between the patients whose surgery had been guided by our self-designed sight and those whose surgery had been not.Results All the patients were followed up for 18 to 36 months (average,22.3 months).Compared with those who had not used the sight,the patients who had used the sight incurred significantly shorter operation time (16.5 ± 3.2 min versus 26.5 ± 3.7 min),significantly less intraoperative bleeding (2.8 ± 1.3 mL versus 6.3 ± 1.7 mL) and significantly less radiation by C-arm roentgenography (5.6 ± 3.3 times versus 9.4 ± 3.1 times) (P <0.05).The VAS scores at postoperative 3 days and final follow-up were significantly decreased than the preoperative values in all the patients (P < 0.05).There were no significant differences between the patients who had used the sight and those who had not in the the VAS scores at postoperative 3 days or final follow-up (P > 0.05).Conclusions The upper thoracic PVP or PKP guided by our self-designed sight has theadvantages of short operation time,less intraoperative bleeding and less frequency of C-arm radiation.The sight is suitable for various vertebroplasties.
10. Anatomical, imaging and histological observations of the circumferential full-length of superficial fascia structure of the upper limb
Yunpeng GU ; Weiwei CHEN ; Xuejian SUN ; Qiang ZHUANG ; Qianwen LYU ; Yue QI ; Zhenjun LIU ; Gui′e MA ; Zuoliang QI
Chinese Journal of Plastic Surgery 2019;35(7):665-670
Objective:
To describe the general and histological features of the full-length superficial fascia of the circumferential upper limb.
Methods:
Fresh frozen arm specimens were dissected, and then MRI imaging in vivo, enhanced CT angiography and HE histological staining were used to describe the characteristics of the full-length superficial fascia of the circumferential arm and its relationship with important blood vessels.
Results:
The four typical structures of the superficial fascia of the arm were divided into subcutaneous superficial fat, membrane-like substance, deep fat and deep fascia from superficial to deep. The thickness and stratification, fusion degree and histological characteristics of the superficial fascia of these four layers were obviously different in different levels and regions of the arm. MRI confirmed that the total thickness of superficial fascia gradually decreased from shoulder to wrist. Venography showed that the cephalic vein ran below the second layer of superficial fascia and above the deep fascia. The basilic vein originated from the dorsal vein network of the hand and always lied below the second layer of membranous material until the basilic vein penetrates below the deep fascia of the upper arm.
Conclusions
The deep understanding of the circumferential full-length of superficial fascia structure of the upper limb provides an important theoretical basis for improving the surgical safety and fine operation for the Dynamic Arm Circumferential Liposuction.