Objective To study comprehensive methods for senile hypertension and compared with conven-tional western medicine treatment,and provide reference to guide clinical hypertension treatment for the future. Methods 162 cases with senile hypertension were selected in our hospital,according to the order of treatment and the principle of informed consent,they were randomly divided into observation group and control group.The control group was given conventional western medicine treatment,the Nifedipine sustained release tablets joint irbesartan tab-lets.Based on the thinkings of health management,we established health records for observation group firstly,then as-sessed cardiovascular risk among patients.The patients with low,moderate risk were conducted the continuous monito-ring of blood pressure,health education,the key intervention in their way of life,established a good mentality.If the blood pressure was still unstable and out of control,we began to consider the use of drug.For patients with high risk or very high risk,besides the treatment considered,they needed immediate medical treatment supplemented by traditional Chinese medicine.Observation group was followed up for 1 time,and lasting for 6 months,then their blood pressure was measured again,the treatment effect and adverse reactions were evaluated.Results The total effective rate of ob-servation group was 96.7% (89 /92),which was significantly higher than that of control group 82.8% (58 /70),χ2 =9.12,P <0.01,the difference was statistically significant.After following up for 6 months,the systolic blood pressure of observation group fell to (126.1 ±21.5 )mmHg,t =7.548,P <0.01;diastolic blood pressure fell to (79.5 ±9.5)mmHg,t =10.703,P <0.01,the change was significantly different than before.The systolic blood pres-sure of control group fell to (145.3 ±26.7)mmHg,t =1.979,P <0.05,there was markedly reduced,diastolic blood pressure decreased too,but there was no statistical difference (P >0.05).So the effect of observation group was better than control group.Meanwhile,some adverse reaction occurred in both groups,but there was no significant difference. Conclusion With the thinking of health management for aged patients with hypertension,offer full -course treatment for them in order to increase the awareness of hypertension and establish healthy lifestyle,guide scientific and rational drug use.The integrated use of these methods can effectively improve the effect of the treatment of senile hyperten-sion,which is worthy of popularization and application.

The development of DNA chip has been strongly driven by modern approaches of life science and microelectronics. The broad of DNA chip applications include the detection of pathogens,the detection of mutations in genes involved in human diseases or affected during cancer progression, sequence analysis, the pattern of gene expression monitoring and pharmacy studies .This review explains the technology, its scope, and impact on medicine, as well as its cost and possible limitations.

Objective To construct a basic competency framework for advanced practice nurses.Methods A primary index system was formed through literature review and expert consultation,the weight of each index was determined by hierarchy analysis.Results The expert authority coefficient was 0.857,Cronbrach alpha of the total system was 0.937.The index system consisted of 5 first-level items,11 second-level items and 46 third-level items.Conclusions The 62-item competency framework for APN is reliable and valid,which can provide quantified standards for selecting,training and evaluating advanced practice nurses.

OBJECTIVE:To investigate the work status of clinical pharmacists in West China Hospital of Sichuan University and to provide rational suggestions for the development and improvement of clinical pharmacy. METHODS: The status of hospital clinical pharmacy and training of clinical pharmacy were studied by a questionnaire survey on 26 clinical pharmacists and an interview on 9 trainees majored in clinical pharmacy. RESULTS: The major tasks for hospital clinical pharmacists were to participate in clinical rounds, conduct pharmacy rounds alone, participate in patients’ treatment and outpatient pharmaceutical care, listen to case discussion, participate case consultation, coordinate with doctors to make medication plan, collect adverse drug reactions and give lecture among physicians etc. The pharmacists were welcome by most of the clinical departments. CONCLUSIONS: Clinical pharmacy in West Hospital of Sichuan University achieved a good beginning, but to achieve a further progress, the focus of clinical pharmacists’ work should be shifted to provide patient-centered pharmaceutical technical service, meanwhile, the support offered and the importance attached by the government as well as the efforts of clinical pharmacists are indispensible.

Objective To observe the protective effect of Naoli Zhibao(NZ) on mice with learning and memory impairment.Methods (1) Thirty-two NIH mice were randomized into pseudo-operation group,model group,and low-and high-dose NZ groups(at the dose of 4 and 8 g?kg-1?d-1 respectively).Except the pseudo-operation group,the mice in other groups received the operation of cerebral ischemia/reperfusion.After treatment for 10 days,the learning and memory abilities of the mice were observed by step-down test,and the contents of malondialdehyde(MDA) and nitric oxide(NO) as well as the activities of superoxide dismustase(SOD) and glutathione peroxidase(GSH-Px) were detected.Meanwhile,the free amino acid content in mice cerebral cortex was measured with high performance liquid chromatography.(2) Forty NIH mice aged 9 months were randomized into normal control group,mimetic aging group,and low-and high-dose NZ groups(at the dose of 4 and 8 g?kg-1?d-1 respectively).The mice models of mimetic aging were induced by intraperitoneal injection of D-galactose 0.5mL for 6 weeks,and the mice in NZ groups were also given gastric gavage of NZ for 6 weeks.After treatment,the learning and memory abilities of the mice were observed by step-down test,and the contents of epinephrine(E),norepinephrine(NE) and tryptophan(TP) in mice cerebral cortex were measured.Results (1) NZ significantly improved the memory ability of mice with cerebral ischemia/reperfusion injury(P

BACKGROUND:The Specification of Quality Management for Medical Device Production (for Trial Implementation) issued by the China Food and Drug Administration (CFDA) on December 16, 2009 was demanded to be initially implemented in medical device manufacturers with a higher risk in sterility and implant before January 1, 2011. The revised version was published on December 29, 2014 and came into effect on March 1, 2015. OBJECTIVE:To put forward specific suggestions through analyzing the status of 31 sterile implantable medical device manufacturers in Sichuan Province and the corresponding implementation situation of the specification for trial implementation. METHODS:The relevant data of these 31 manufacturers were collected through the quality management system assessment, supervision and inspection, flight inspection and questionnaire survey. And the data were analyzed statistically. RESULTS AND CONCLUSION:The overall size of medical device manufacturers in Sichuan Province is small. There are still some other problems, such as no advanced equipment, low quality of employees, lack of funds and inappropriate management, in these manufacturers. Considering these, we have given some guiding recommendations from the aspects of production and supervision.

Objective To investigate the expression of C/EBP homology protein (CHOP) in peripheral brain tissue of patients with severe traumatic brain injury (TBI) and its correlation with the injury severity.Methods The study included peripheral brain tissues of 41 TBI patients (TBI group).Another 16 autopsy specimens succumbed to other diseases (except for TBI or other central nervous system diseases) were selected as controls.The control group and TBI group were subdivided into immaturity group (≤18 years),adult group (18-59 years) and elderly group (＞59 years).According to Glasgow Coma Scale (GCS) on admission,TBI group was classified as severe TBI group (GCS of 6-8) and particularly-severe TBI group (GCS of 3-5).CHOP expression in peripheral tissues after TBI was compared in between different age,gender and GCS.Nerve cell apoptosis was detected by TUNEL technique and correlation between CHOP level and apoptotic number was analyzed.Results There were no age and gender differences regarding CHOP expression in control group (P ＜ 0.05).Compared with control group,expression of CHOP presented notable up-regulation in TBI group (P ＜ 0.05).Expression of CHOP presented no gender difference in TBI group (P ＞ 0.05),but its expression was lower in the aged than in adult or immaturity (P ＜ 0.05) as well as notably higher in particularly-severe TBI group than in severe TBI group (P ＜ 0.05).Nerve cell apoptosis in TBI group was far greater in number than that in control group (P ＜0.05).A positive correlation was observed between CHOP level and apoptotic index (r =0.72,P ＜ 0.05).Conclusion Expression level of CHOP after TBI is closely related to the injury severity and nerve cell apoptosis,but the apoptosis pathway induced by CHOP may not be a major factor in secondary brain injury after TBI in the aged patients.

Objective To evaluate the clinical effects of sitagliptin and sitagliptin combined with glimepiride in the treatment of type 2 diabetes (T2DM).Methods Ninety-two patients with T2DM were randomly divided into sitagliptin group (group J),glimepiride group (group G) and sitagliptin combined with glimepiride group (group U),group J took sitagliptin,group G took glimepiride,group U took sitagliptin and glimepiride.Before and after treatments,blood glucose and insulin were determined in the fasting and 2-hour blood samples after taking glucose (fasting blood-glucose (FPG),2-hour postprandial blood glucose (2hPG),insulin (FIns),2-hour postprandial insulin (2hIns),and glycosylation hemoglobin (HBA1 c) were also determined and homeostasis model assessment was applied to estimate the functions index of islet β cell(HOMA-β).Results The levels of blood glucose and HBA1C in three groups decreased after treatments(FPG,(before treatment:(9.2±3.0),(9.2±2.8),(9.3±3.2) mmol/L),(after treatment:(7.7 ± 3.0),(6.9 ± 2.6),(6.0 ± 2.5) mmol/L),and t values are 2.205,3.203,3.691,P ＜ 0.01,P ＜ 0.05 ;2 hPG (before treatment:(14.1 ± 5.7),(14.8 ±6.3),(15.0±6.8) mmol/L),(after treatment:(7.9 ±2.9),(9.0 ±3.1),(7.1 ±3.1) mmol/L),and t values are 3.881,3.159,4.189,P ＜ 0.01 ; HBA1c (before treatment:(8.52 ± 2.01)％,(8.48 ± 1.94)％,(8.56 ±2.27)％,(after treatment:(7.64 ± 1.92)％,(6.81 ± 1.55)％,(6.19 ± 1.84)％),t values are 2.292,2.184,3.269,P ＜ 0.01,P ＜ 0.05) ; HOMA-β in the three groups increased after treatment ((before treatment:1.42 ± 0.07,1.44 ± 0.06,1.41 ± 0.11),(after treatment:1.76 ± 0.14,1.68 ± 0.20,1.85 ±0.17),t values are 2.180,2.073,2.882,P ＜ 0.01,P ＜ 0.05);levels of HBA1c and blood glucose in group U were lower than those in group J and G(HBA1 c:t values are 2.785,2.138,P ＜ 0.05,P ＜ 0.01 ;FPG:t values are 2.252,2.346,P ＜0.05;2hPG:t values are 2.147,2.829,P ＜0.01,P ＜0.05),HOMA-β in which was higher than that of group G(t =2.153,P ＜ 0.05),but with no significant difference compared with group J (t =1.796,P ＞ 0.05),levels of HBA1C,FPG and HOMA-β in group J were higher than those of group G (t values are 2.108,2.202,2.121,P ＜ 0.05),level of 2hPG of group J was lower than that of group G(t =2.307,P ＜ 0.05).Conclusion Sitagliptin provides significant glycaemic control,together with glimepiride,clinical effect of treatment of type 2 diabetes will be enhanced.

Objective In order to standardize plantation and get bumper crops cultivation technique of Abrus cantoniensis Hance (a general term: "Herba Abri"). Methods To cultivate A. cantonients guiding by the instructive rules of productive quality and standard of management of Chinese medicinal materials, productive A. cantoniensis under good agriculture practice (GAP) promulgated by State Administration of Food and Drug. Results Comparing to production under non-GAP, the content of extract and flavones was higher, while the content of pesticide residue and heavy metal was lower under GAP. Conclusion Plantation of A. cantoniensis under GAP through choosing soil strictly, selecting seeds, optimizing pollution-free environment and friendly fertilizer and agriculture chemical, the harmful heavy metal and residues of pesticides amount in crude medicinal materials could be controled effectively so that the quality and output of A. cantoniensis could be improved.

OBJECTIVE:To observe the efficacy and safety of Guizhi fuling capsule combined with mifepristone in treatment of uterine fibroids. METHODS:116 patients with uterine fibroids were randomly divided into control group and observation group. Control group was orally given Mifepristone tablet 25 mg,2 h before meal in the first day of menstrual period,once a day,for con-tinuous 10 d;observation group was additionally given Guizhi fuling capsule 3 pills in non-menstrual period,3 times a day. 3 months was regarded as a treatment course,it lasted 2 courses. Clinical efficacy,and E2,FSH,SHBG,uterine volume,menstrual blood volume,uterine fibroid volume and incidence of adverse reactions in 2 groups before and after treatment were observed. RE-SULTS:Total effective rate in observation group was significantly higher than control group,the difference was statistically signifi-cant(P<0.05). After treatment,the FSH,E2,uterine volume,menstrual blood volume and uterine fibroid volume in 2 groups were significantly lower than before,and observation group was lower than control group,the differences were statistically signifi-cant(P<0.05);but there were no significant difference in the SHBG and adverse reactions between 2 groups(P>0.05). CONCLU-SIONS:The clinical efficacy of Guizhi fuling capsule combined with mifepristone in treatment of uterine fibroids is more signifi-cant than mifepristone alone,with good safety.