The method, established for analysis of paeoniflorin in blood plasma, appeared a good linear relationship(r=0.9998). Using meta-hydroxybenzoic acid as internal standard and calibration factor combined, the quantitation precision of method could be raised. The result of determination of calibration factor conformed with the chromatography prescriptions in "Pharmacopoeia of China" (edited in 1990). The average recovery reached up 97.13％. The minimum detection amount was 5.6ng. The coefficients of within-day and between-day variation were 0.39％ and 1.17％, respectively. This method is suitable for paeoniflorin analysis of in vivo sample . At present,the in vivo absorption of Baihegujintang Granules (containing Radix Paeoniae alba or Radix Paeoniae rubra) by rabbit is preliminarily investigated utilizing this method.

duodenum, respectively. Conclusion The absorption of tetrandrine is first-order process with passive diffusion mechanism. Tetrandrine is well absorbed at all segments of intestine in rats.

OBJECTIVE: To comprehend the general situation of pharmaceutical services in hospitals in China.METHOD: The general situation of pharmaceutical services was investigated by questionnaire survey through mailing among a total of 600 hospitals including third-level grade A and B and second-level grade A and B hospitals and 42 pilot sites hospitals of clinical pharmacist work system selected by stratified random sampling.And the results were given a statistical analysis.RESULTS & CONCLUSIONS: A total of 281 questionnaires(46.8%) were sent back from sample hospitals and 29(69.0%) from pilot hospitals.37.1% of the hospitals developed the service of mixed dispensing for intravenous drugs,61.7% set up drug information consultation department,32.9% had monitoring software or system for rational drug use,indicating a great evolvement has been achieved in Chinese hospital pharmaceutical services.But there is still great difference in pharmaceutical care between different level and grade of hospitals.

OBJECTIVE: To probe into the status quo of the practice of clinical pharmacy in hospitals of China.METHODS: A mailed questionnaire survey was conducted among 600 sample hospitals and 42 clinical pharmacist work system pilot hospitals based on stratified sampling regarding the status quo of the clinical pharmacy practice in China.And the results were subjected to statistical analysis.RESULTS & CONCLUSIONS: A total of 310 questionnaires were sent back,which revealed that the proportions of developing clinical pharmaceutical services including prescription evaluation,ADR monitoring,drug information consultation in hospitals at different level were high,but the proportions of developing such pharmaceutical services as medication orders' writing and drug-food interaction monitoring were low.The pilot hospitals had better clinical pharmacy services as compared with common hospitals,much as in drug therapy monitoring and individualized medication therapy.The clinical pharmacists were characterized by low quality and scanty numbers.The financial support to clinical pharmacy and the role of the clinical pharmacists remain to be intensified.

OBJECTIVE:To study the use and developmental tendency of psychotolytics in hospitals of Guangdong province.METHODS:The psychotolytics used in hospitals of Guangdong province during the period of2000～2004were an-alyzed statistically in respect to consumption sum,DDDs and average daily expense.RESULTS&CONCLUSIONS:Both consumption sum and DDDs of psychotolytics in the past5years increased to a large extent.Benzodiazepine topped the list of anti-anxiety drugs.The consumption of2nd generation antipsychotics increased year by year.On the whole,the consumption of antidepressants increased annually,of which,fluoxetine,flupentixol/melitracen and paroxetine were the most frequently used drugs.However,the consumption of tricyclic antidepressants has a significant reduction.

OBJECTIVE:To study the pharmacokinetics of aclacinomycin A polylactide lyophilized nanopartical (ACM-A-PLA-NP) in plasma of rabbit.METHODS:HPLC was selected to determine the concentration of aclacinomycin A in plasma after a single dose of ACM-A-PLA-NP and lyophilized ACM-A.RESULTS:The pharmacokinetic parameters of these two dosage forms were obtained by 3p87.CONCLUSION:The lyophilized ACM-A-PLA-NP has a more sustained release character comparing with the lyophilized ACM-A.

AIM：To introduce evidence-based pharmacy in respect to the definition,contents and principle in applying to decisions of clinical pharmacotherapy.METHODS：We focused the discussion on a brief summary of different study designs,factors affecting the strength of scientific evidence,and interpretation of the data.RESULTS：These application principles are helpful to guide clinical pharmacists solving clinical problems.CONCLUSION：Clinical pharmacist should use concepts in the evidence-based practice when making pharmacotherapeutic decisions in the future.

This paper reported TLC identification of various Chinese medicines in Renshensinitang Oral Liq- uid,limit dose detection and content determination of aconitine.These methods could be available for internal quality control of Renshensinitang Oral Liquid.

Objective To observe the protective effect of Naoli Zhibao(NZ) on mice with learning and memory impairment.Methods (1) Thirty-two NIH mice were randomized into pseudo-operation group,model group,and low-and high-dose NZ groups(at the dose of 4 and 8 g?kg-1?d-1 respectively).Except the pseudo-operation group,the mice in other groups received the operation of cerebral ischemia/reperfusion.After treatment for 10 days,the learning and memory abilities of the mice were observed by step-down test,and the contents of malondialdehyde(MDA) and nitric oxide(NO) as well as the activities of superoxide dismustase(SOD) and glutathione peroxidase(GSH-Px) were detected.Meanwhile,the free amino acid content in mice cerebral cortex was measured with high performance liquid chromatography.(2) Forty NIH mice aged 9 months were randomized into normal control group,mimetic aging group,and low-and high-dose NZ groups(at the dose of 4 and 8 g?kg-1?d-1 respectively).The mice models of mimetic aging were induced by intraperitoneal injection of D-galactose 0.5mL for 6 weeks,and the mice in NZ groups were also given gastric gavage of NZ for 6 weeks.After treatment,the learning and memory abilities of the mice were observed by step-down test,and the contents of epinephrine(E),norepinephrine(NE) and tryptophan(TP) in mice cerebral cortex were measured.Results (1) NZ significantly improved the memory ability of mice with cerebral ischemia/reperfusion injury(P

Objective To establish a method for calculating deferral periods of blood donors having taken traditional Chinese medicines based on the drug's pharmacokinetics.Methods The pharmacokinetic method was used.For drugs that are not known to cause anaphylaxis or teratogenesis,the interval between last dose of drug and safe blood donation equals to t max plus 7t 1/2 .For drugs with known teratogenic and anaphylactic risks,a deferral period of 20 plasma elimination half lives and t maxis necessary.There are some rules independent of the drug's half life.Results 22 intervals of traditional Chinese medicine are determined.Conclusion Our recommendations for deferral periods of blood donors after traditional Chinese medicine treatment,based on pharmacokinetics can increase the safety of donated blood.