Objective To establish the HPLC fingerprint of amino acids compounds from Buzhong Yiqi Pill.Methods A pre-column derivatization HPLC method was adopted with praline as a reference.Separation was performed on a AccQ? TagTM C18(3.9? 150 mm)analytical column with mobile phase consisting of 0.15 mol/L sodium acetate buffer solution and 60 % acetonitrile,gradient elution with the flow rate of 1.0 mL/min.The column temperature was at 37 ℃.The Fluorescence detecting wavelength was set at 250 nm(excitation wavelength)and 395 nm(emission wavelength),the analysis time was 35 min.Results Twelve common peaks on the HPLC fingerprints of Buzhong Yiqi Pill were presented.The similarity was determined by the correlation coefficient,included angle cosine coefficient and evaluation software of chromatogram fingerprint similarity.The results showed the similarity for 10 bathes of samples was in 0.97～ 1.00.Conclusion Good fingerprints were obtained,which can be used for the quality control of Buzhong Yiqi Pill.

Objective To explore the effects of multidisciplinary comprehensive pulmonary rehabilitation on aged patients with pneumo-coniosis. Methods From January, 2012 to January, 2015, 85 old stable inpatients with pneumoconiosis were divided into control group (n=40) and rehabilitation group (n=45). Both groups accepted routine medicine, while the rehabilitation group received multidisciplinary com-prehensive pulmonary rehabilitation for twelve weeks. They were assessed with cardiopulmonary exercise test, BODE index and quality of life before and after treatment. Results The maximum work, maximal oxygen uptake, 6-minute walk distance, scores of modified Version of the British Medical Researsh Council Respiratory Questionnaire, BODE index, activities of daily living, social activities, depression and anx-iety improved in the rehabilitation group after treatment (t>3.379, P<0.01), and were better than those in the control group (t>2.201, P<0.05). No imporvement was found in all the indices in the control group after treatment (t<2.339, P>0.05). Conclusion Multidisciplinary comprehensive pulmonary rehabilitation can effectively improve exercise tolerance and quality of life in aged patients with pneumoconiosis.

Objective To study the change of Hesperidin content in various combinations of medicine from Buzhong Yiqi Decoction(BYD) by RP-HPLC.Methods The hesperidin content was determined by HPLC and analyzed by L8(27) orthogonal design and statistic analysis(SPSS).Results Principal herbs and ministerial herbs of BYD had a significant effect on the hesperidin content of adjunctive herbs(P

Objective To investigate the predictive value of ultrasonic tissue charateristics of carotid atherosclerotic plaques in cardiocerebrovascular events in patients with type 2 diabetes mellitus (T2DM).Methods From January 2003 to December 2015,patients with T2DM treated in Xi'an Gaoxin Hospital were selected.Their carotid atherosclerotic plaques were examined by ultrasound.Intergrated backscatter (IBS) and thickness of carotid plaques of the patients were documented.The cardiocerebrovascular events were folbwed up prospectively.Kaplan-Meier curve,COX proportional risk regression model,and receiver operating characteristic (ROC) curve were used to compare and analyze the predictive values of calibratedIBS (C-IBS),Framingham Risk Score (FRS) and plaque thickness for the risk of cardiocerebrovascular events in patients with T2DM.Results There was significant difference in the risk of cardiovascular events between the low C-IBS group (n=48,C-IBS ＜17.1 dB) and the high C-IBS group (n=48,C-IBS ≥17.1 dB) (35.4％ vs.12.5％;P=0.015).There was no significant difference in the risk of cardiocerebrovascular events between the thin plaque group (n =48,plaque thickness ＜ 1.3 mm) and the thick plaque group (n=48,plaque thickness ＞1.3 mm) (14.6％ vs.33.3％;P=0.070).COX proportional risk regression model analysis showed that C-IBS (hazard ratio,1.505,95％ confidence interval 1.196-1.893,P=0.001) and plaque thickness (hazard ratio,4.794,95％ confidence interval 1.142-20.130,P =0.032) were the independent risk factors for the risk of cardiocerebrovascular events.ROC curve analysis found that the combination of C-IBS,FRS and plaque thickness could predict the risk of cardiocerebrovascular events in patients with T2DM.Conclusion Ultrasonic tissue characteristics of carotid atherosclerotic plaque (C-IBS and plaque thickness) in combination with FRS may predict cardiocerebrovascular events in patients with T2DM.