Objective To analyze the parametric errors during the use of Sysmex CA-1500 automated coagulation analyzer and their treatment measures .Methods Blood samples with test results showing “*”and without figure results were collected .Sysmex CA-1500 automated coagulation analyzer was employed to retest prothrombin time (PT ) ,activated partial thromboplastin time (APTT) and fibrinogen(FIB) .Results FIB ,PT and APTT were found abnormal in 226 cases(95 .77% ) ,5 cases(2 .12% ) and 5 cases(2 .12% ) ,respectively ,with FIB the mainly .ERR008 ,ERR004 ,ERR016 ,ERR002 ,ERR032 and ERR100 were found abnormal in 227 cases(76 .95% ) ,64 cases(21 .69% ) ,one case(0 .34% ) ,one case(0 .34% ) ,one case(0 .34% ) and one case(0 .34% ) ,respec-tively ,with ERR008 the mainly .Difference of no-error results between the initial and repeat test of PT and APTT showed no statis-tical significance(P>0 .05) ,while those of FIB demonstrated statistical significance (P<0 .01) .Conclusion Samples with paramet-ric errors during the use of Sysmex CA-1500 automated coagulation analyzer should be retested to promote the quality of blood co-agulation detection .

Objective To explore the inhibitory effect of the ethanol extract from peanut root on the non-castration adult rat prostate hyperplasia induced by testosterone propionate and its mechanism .Methods 60 SD rats were randomly divided into the control ,model and high ,middle and low dose of peanut root ethanol extract treatment groups ,among them ,the control group was normally fed without medication ;the model group was subcutaneously injected by testosterone propionate (TP ,5 mL/kg/d) and simultaneously gavaged subcutaneous injection with peanut root ethanol extract (10 mL/kg/d ,5 mLg/kg/d or 1 mL/kg/d) for suc-cessive 14 d .The rats in various groups were killed on 15 d and their prostate ,spermatophore and testicle tissues were separated and weighed .The ratio of gland/body-weight(mg/g) was calculated .The expressions of Bcl-2 and Bax proteins in the prostate tissues were detected by immunohistochemistry .Results There was statistical difference in the indexes of prostate between the treatment groups(high and middle dose) and the model group(P<0 .01) ,while there was no statistical difference between the low dose group and the model group(P>0 .05) .This result showed that there was a dose-effect relationship between the inhibition effect on rat prostate hyperplasia and the ethanol extracts from peanut root .There was no statistical differences between the model group and the treatment groups in the indexes of testicle ,spermatophore or the change of weight (P>0 .05) .There was statistical difference be-tween the control group or model group and the treatment group in the expression of Bcl-2 or Bax protein(P<0 .05) ,while no sta-tistical difference between the treatment group and the control group (P>0 .05) .Conclusion Ethanol extract from peanut root has good inhibiting effect on prostate hyperplasia and its mechanism could reach the curative effect by regulating the balance relation be-tween apoptosis gene Bcl-2 and Bax protein proportion and promoting apopitosis of benign prostatic hyperplasia .

Objective To explore the changes of cerebral vasoreactivity(CVR) in patients with acute cerebral infarction of various clinical subtypes.Methods The 70 patients with acute cerebral infarction were divided into 3 subtypes:atherothrombotic infarction (AI)group(n=22), lacunar infarction (LI)group(n=33), cardiogenic infarction (CI)group(n=15).The mean blood flow velocity (Vm), pulse index(PI), resistance index(RI) in bilateral middle cerebral artery(MCA) were detected, and breath holding index (BHI) was measured by TCD in these patients through breath holding test. All the data were compared with the health controls. Results Compared with normal control group,the Vm,PI and RI were significantly increased (P0.05). Conclusions There are differents in CVR in patients with acute cerebral infarction of various subtypes. The damage of CVR is more obviously in AI and LI group. There is clinical significance for measurement of CVR to evaluate the haemodynamic changes in various subtype of acute cerebral infarction.

Objective To assess the cerebrovascular reactivity (CVR) in health subjects and patients with risk factors by means of transcranial Doppler ultrasonography (TCD) with breath-holding maneuver, and its association with risk factors of cerebrovascular disease.Methods The breath-holding index (BHI), which was the percentage increase in middle cerebral artery(MCA)blood flow velocity and was as index of CVR assessment, was detected during breath-holding by TCD and breath-holding technique in 137 patients with various risk-factors of cerebrovascular disease and 87 normal controls.The regression analysis was done between the CVR and risk factors.Results The mean blood velocity before (Vm) and after holding test (Vm′) were significantly lower in group with age of 20-40 years than that of the age of 41-60 and over 60 years(all P

Objective To assess the effects of 7,8-dihydroxyflavone (7,8-DHF) on the striatum (ST) in normal cynomolgus monkeys using 99Tcm-TRODAT-1 imaging.Methods A total of six healthy female cynomolgus monkeys were included in this study.Three of them were fed with normal food (control group),and the other three were given oral administration of 7,8-DHF in addition to normal food (experimental group).The SPECT/CT imaging was performed at different time after 99Tcm-TRODAT-1 injection.The ROI of ST was drawn on images of 3 consecutive transverse slices that could be visualized best.The cerebellum (CB) was taken as the background reference area.The radioactivity uptake ratios of ST/CB at 1,3,4 and 5 h were calculated respectively.Paired-t test was used to analyze the data.Results ST radioactive uptake ratios showed continuing increase on the delay images.ST/CB uptake ratios of the control group at 1,3,4 and 5 h were 1.43±0.04,1.82±0.06,2.04±0.12,2.42±0.23,respectively,and those of the experimental group were 1.35±0.08,2.40±0.09,2.74±0.13 and 3.25±0.15 respectively.There was no significant difference between the two groups at 1 h (t =2.57,P＞0.05),while ST/CB uptake ratios of the experimental group at 3,4 and 5 h were significantly higher (t values:2.77,2.87 and 2.92,all P＜0.05).Conclusion 99Tcm-TRODAT-1 SPECT/CT imaging can be used to assess the DAT activation effect by 7,8-DHF on ST of cynomolgus monkeys.

The authors compared the purpose,and the main content of three international standards in medical education developed by the Institute for International Medical Education (IIME), World Health Organization Western Pacific Regional Office (WHO/WPRO), and World Federation for Medical Education (WFME) respectively. The IIME's standard is deferent from the others. The IIME's standard contains seven broad educational outcome domains and 60 items in the domains. The purpose of IIME's standard is to improve the common core competency of individual medical graduates of each medical school in the world, and the standard focuses the outcome of medical education and belongs to summative individual evaluation. The WHO/WPRO's and WFME's standards are quite similar. They define the standards across nine broad areas of medical schooling divided into 38 sub-areas. The ultimate goal of the WHO/WPRO's standard is to encourage national governments to adopt a quality assurance process in medical education. The aim of the two standards is to promote the quality assurance of medical schools. Both of the two standards focus the whole process of medical schooling and medical schools, and belong to formative evaluation.

Objective To investigate the correlation of left ventricular ejection fraction (LVEF) as well as serum levels of NT-proBNP, Hcy and D-Dimer (D-D) with different traditional Chinese medicine (TCM) syndrome types of chronic heart failure (CHF). Methods A total of 178 CHF patients were divided into heart function normal ejection fracture group (HF?NEF, n=86) and heart function reduction (HFREF, n=92) according to their LVEF performance. Another 35 cases with nor?mal cardiac function were included in control group. All CHF patients was also divided into 3 TCM syndrome types:both de?ficiency of Qi and Yin syndrome group(n=64),Qi asthenia causing blood stasis syndrome group(n=59) andYang defi?ciency water stop group (n=55). All patients were examined with cardiac color doppler and LVEF values were recorded. And serum NT-proBNP、Hcyand D-D levels were all quantified. Results As to serum levels of NT-proBNP, Hcy and D-D, they were higher in HFREF group than those in HFNEF group than those in control group. On the other hand, LVEF was lowest in HFREF group but highest in control group. All differences were statistically significant (P<0.05). Among patient in HFNEF group, LVEF in theYang deficiency water stop groupwas lower than that inboth deficiency of Qi and Yin syn?drome group(P<0.05). Serum levels of NT-proBNP, Hcy, and D-D were not significantly different between different TCM syndrome groups. By contrast, among patients in HFREF group, LVEF values did not differ significantly between different TCM syndrome groups. Serum level of NT-proBNP was lower inboth deficiency of Qi and Yin syndrome groupthan that inQi asthenia causing blood stasis syndrome group than that in Yang deficiency water stop group. As to serum levels of Hcy and D-D, they are higher inYang deficiency water stop groupthat those inboth deficiency of Qi and Yin syndrome group and Qi asthenia causing blood stasis syndrome group(P<0.05). Conclusion Patients with different TCM syn?drome types of CHF present different levels of serum NT-proBNP, Hcy, D-D level and LVEF. Changes of indicators in HFREF groups are more obvious than they did in HFNEF group.

Breathing gas carries important physiological information. Technology for detection of breathing gas has become a research focus because of the advantages of nondestructive sampling and convenient operation. Proton transfer reaction mass spectrometry (PTR-MS) plays an irreplaceable role because of the advantages of high sensitivity, fast response and good specificity. In this paper, the principle of PTR-MS is introduced first, followed by research progress of PTR-MS in the field of breathing gas detection. Factors influencing the test results are analyzed. Finally, future prospects of development for PTR-MS in the field of breathing gas detection are discussed.
Gases ; isolation & purification ; Mass Spectrometry ; methods ; Protons

The different effects of capsaicin on I(A) and I(K) currents in pain-conduct neurons of trigeminal ganglia (TG) were investigated. In cultured TG neurons of rats, whole-cell patch clamp techniques were used to record the I(A) and I(K) before and after capsaicin perfused. Results revealed that 1 micromol/L capsaicin could inhibit the amplitude of I(A) by 48.2% (n = 10, P < 0.05), but had no inhibitory effect on I(K) (n = 7, P > 0.05). Ten micromol/L capsaicin could significantly inhibit the amplitude of I(A) by 93.2% (n = 8, P < 0.01), but only slightly inhibit the amplitude of I(K) by 13.2% (n = 7, P < 0.05). Neither 1 micromol/L nor 10 micromol/L capsaicin had effects on the active curve of I(A) and I(K). It was concluded that capsaicin could selectively inhibit the I(A) current, and this effect might involve in the analgesic mechanisms of capsaicin.

Objective: To explore the effect of cognitive-behavioral therapy combined with family rehabilitation therapy on improving depression patients. Methods: Totally 82 depressive patients admitted to our hospital from February 2016 to January 2017 were randomly divided into the control group and the observation group according to the random number table method, with 41 cases in each group. Patients in the control group were treated with family rehabilitation therapy, while the patients in the observation group were treated with cognitive behavioral therapy on the basis of family rehabilitation therapy. After 12 months of intervention, the patients in the observation group were treated with cognitive behavioral therapy. The treatment efficiency, psychological status, coping style and medication compliance of the two groups were compared. Results: After intervention the scores of Self-rating Depression Scale (SDS) and Hamilton Depression Scale (HAMD) in the observation group were (31.08±4.83), (14.14±3.52) points respectively, which were significantly lower than (36.97±2.73), (16.68 ±2.14) points of the control group (t=-6.789,-3.948, P < 0.01). The effective rate of the observation group was 97.56% (40/41), which was significantly higher than 70.05% (32/41) of the control group, and the difference was statistically significant (χ²=5.581, P<0.05). The scores of "problem solving" and "help seeking" items in the observation group were (1.66±0.18) and (1.81±0.21) respectively, significantly higher than (1.39±0.11), (1.48±0.13) of the control group; "avoidance" and "self-blame" items were (1.33±0.12) and (1.47±0.14) respectively, significantly lower than (1.58±0.19), (1.48±0.13) in the control group, and the difference was statistically significant (t=-8.349-8.555, P < 0.05). After treatment, the compliance of the observation group was significantly better than that of the control group (χ²= 8.902, P < 0.05). Conclusion Cognitive behavioral therapy combined with family rehabilitation therapy for depression patients can effectively improve the bad mood of patients, improve their medication compliance, and achieve better clinical treatment results.