OBJECTIVE:To evaluate the bioequivalence of compound fluamine tablets with commercial fluamine tablets and glibenclamide tablets(R).METHODS:HPLC method was used to determine the plasma drug time-concentrations of flu?amine and glibenclamide separately.The pharmacokinetic parameters were calculated and analysed with variance and two-one side t-test.RESULTS:The T 1/2 ,T max ,C max and AUC 0～24 for fluamine of test and reference formulation were(7.969?0.713)h and(8.103?0.596)h;(1.889?0.214)h and(1.876?0.207)h;(2.001?0.368)?g/ml and(1.962?0.416)?g/ml;(10.706?1.424)(?g?h)/ml and(10.624?1.317)(?g?h)/ml respectively.F was(100.97?7.73)%.The T 1/2 ,T max ,and AUC 0～24 for glibenclamide of test and reference formulation were(6.536?0.890)h and(6.629?0.563)h;(4.038?0.309)h and(4.056?0.338)h;(98.026?22.790)ng/ml and(97.507?20.529)ng/ml;(510.702?135.818)(ng?h)/ml and(499.452?111.328)(ng?h)/ml,respectively,and F was(102.066?12.405)%.CONCLUSION:The statistical analysis showed that com?pound fluamine tablets and commercial fluamine tablets and glibenclamide tablets were bioequivalent.

Objective To investigate the neurofilament protein (NF) and synaptophysin(SYN) distribution in the developing stage of human embryonic spinal cord and their clinical significance . Methods Totally 16 of human embryos, aged from 2 to 4 months were used in this study .Immunohistochemical staining was uesd to detect the expression and distribution of NF and SYN in the spinal cord .Results At the second month stage , NF expression was weakly positive in only the marginal layer on both sides of the spinal cord .At 3-4 months, NF expression was positive or strong positive in the spinal cord center crack before and after the middle of partition and cortex .In the ependymal layer of the lateral part of the expression of NF positive nerve fibers were seen in the gray matter .From 2 to 4 month-age, all visible SYN positive expressions distributed in the spinal cord .With the increase of gestational age , SYN positive expression in the spinal cord staining intensity value was increased first , and then decreased .The number of positive values increased month by month . Conclusion NF and SYN both take part in the regulation of human embryonic spinal cord tissue growth .

Objective To study the expressions of Connexin43 (Cx43) and B-cell lymphoma/leukemia-2 (Bcl-2) proteins in the ventricular myocardium of human fetus,and explore the correlation between the expression of the two proteins and the development of fetus myocardium.MethodsThe expressions of Cx43 and Bcl-2 protein in the ventricular myocardium of human embryos were immunohistochemically detected in the second,third and fourth month of gestation,respectively,by strepto-avidin-biotin-peroxidase complex (SABC) and avidin-biotin-peroxidase complex (ABC) technics.ResultsThe positive expression of Bcl-2 protein was detected in one or two cells in the ventricular myocardium of human embryos in the second month of gestation.With the advancement of fetal age,the number of Bcl-2 positive cells increased gradually and distributed in clusters in the ventricular myocardium of human fetus in the fourth month of gestation.The cells with positive expression of Cx43 protein were detected and scattered in dots-like structure in the cytoplasm of myocardial cells in the ventricular myocardium of human embryos in the second month of gestation.With the advancement of fetal age,the distributing region of Cx43 protein extended gradually,and the Cx43 protein positively expressed in the membranes between ventricular myocardial cells.The positively expressed Cx43 protein showed a diffused distribution in a sheet form in the ventricular wall of human fetus in the fourth month of gestation.ConclusionCx43 and Bcl-2 proteins participate in the early growth and development of the ventricular myocardial cells of human fetus.

To improve the CT diagnosis of cerebral angioreticuloma, 3 cases of pathologically proven cerebral angioreticuloma were reviewed. The results showed that the image of CT of the first case revealed an irregular, parenchymatous mass located in the right temporallobe and middle cranial fossa.In the second patient,the image showed a annular mass with cystic consistency,surrounded by an area of edema, situated in the right frontal lobe,while in the third patient there was an irregular high density mass with an area of edema lying in the left frontal lobe and along the middle line. Pathological diagnosis of the first patient was mixed angioreticuloma and glioblastoma, and that of the others were angioreticulomas. the results suggested that if the mass in the cerebrum was obviously enhanced in CT, the diagnosis of angioreticuloma should be considered.

AIM: To explore the effects of high glucose a t fluctuant concentrations on glucose transport activity and expression of phosp hoinositide 3-kinase (p85) in primary cultured skeletal muscle cells. ME T HODS: Rat skeletal muscle cells were cultured at fluctuant glucose conce ntrations (5, 25mmol/L) for 48h. Then the glucose uptake and the expression of p hosphoinositude 3-kinase were measured. RESULTS: The skeletal mu s cle cells treated with fluctuant high concentration glucose showed the impairmen t of the basal and insulin-induced increase in glucose uptake and significant de crease in p85 protein expression as well as p85 mRNA. Particularly, there was a significant decrease in p85 protein and mRNA expression in the high fluctuate l evel of glucose concentration. CONCLUSION: The exposure to fluct uant high concentration of glucose inhibits glucose uptake and induces insulin r esistance in skeletal muscle cells.

Objective To discuss the influence of nursing intervention on sleep quality and postoperative comfort of patients with percutaneous coronary intervention(PCI). Methods We divided 96 patients who were to receive PCI into the test group and control group with 48 patients in each group.The control group received routine nursing while the test group were given systemic nursing intervention based on routine nursing intervention.The sleep quality and incidence rate of complications were compared. Results The sleep quality in the test group was better than that of the control group (P ＜ 0.01 ).The ineidience of waist-back pain,dysuria,induced emiction,eatheter emietion,anaesthesia of lower limbs,fidget in the test group also decreased compared with the control group(P ＜ 0.05). Conclusions Systemic nursing intervention could improve the sleep quality and postoperative comfort for PCI patients

Objective To explore the rule of cell proliferation,apoptosis and expression significance of involved proteins as Bcl-2 and Bax in the developing esophagus of human fetus. Methods In the second through the fourth month of gestation,the expressions of Bcl-2 and Bax proteins were observed in 14 human esophagus using the ABC of immunohistochemical methods. Results In the second,the third and the fourth months of gestation,there were Bcl-2 positive cells distributed in the intermuscular and submucous nerve plexus,and the mucous epithelial cells in the human esophagus.Bax weakly positive cells were observed in the mucous epithelial cells of the human esophagus.Conclusion Bcl-2 and Bax proteins are closely related with the developing esophagus of human embryo.

Objective To explore the rule of distribution and the expression significance of neuronal nitric oxide synthase(nNOS),paired box 3(Pax3) and connexin 43(Cx43) proteins in the early developing spinal cord of human embryos. Methods During the 5th to 16th weeks of human gestation,the expression productions of nNOS,Pax3 and Cx43 proteins were investigated in the anterior horn of spinal cord using the immunohistochemical methods. Results During the 5th to 16th weeks of human gestation,the immunohistochemical staining intensities of both nNOS and Pax3 in the anterior horn neurons of spinal cord increased from weakly to strongly positive staining.During the 5th to 12th weeks of human gestation,the immunohistochemical negative staining of Cx43 protein was observed in the anterior horn neurons of spinal cord,while the positive staining was observed from the 13th to 16th weeks.Conclusion nNOS,Pax3 and Cx43 proteins are related to the growth and development of spinal cord in human embryos.

10.3969/j.issn.2095-4344.2013.24.024

Respiration is one of the most important modulators of heart rate variability(HRV). The modulation mechanism of respiration on HRV were introduced. The effects of respiratory rate, respiratory volume and respiratory patterns on HRV were discussed, and these effects on HRV in patients were also referred. These respiratory effects should be taken into account when HRV is used to predict cardiac vulnerability.