The effects of ?-carotene from Dunaliella salina(?-C) at three doses(12.5,25 and 50 mg?kg~(-1),ip) on learning and memory in rats and mice were studied by using step-down test and Y-maze test.In step-down tests,?-C at all three tested doses had significant effects on normal mice,and could remarkably antagonize the memory impairment induced by scopolamine and 20 % alcohol,but could not antagonize the impairment induced by sodium nitrite.As the same result,?-C at three tested doses administration had significant effects on rats in Y-maze tests.These results suggested that ?-C as an antioxidant could improve the ability of learning and memory in rats and mice.

[ Objective ] To establish a method for the determination of serum strychnine concentration in rheumatoid arthritis patients treated with Tongbiling prescription and to monitor the serum strychnine concentration. [ Methods ] Serum strychnine was extracted with solid phase extraction column. Reversed-phase HPLC conditions were: Hypersil Division ODS C18 column (150 mm ?4.6 mm, 5?m), mobile phrase being methanol: water (70 : 30) with 0.6 g potassium dihydrogen phosphate and 1.0 g dodecyl sodium sulfonate added in 1000 mL mixture (adjust pH to 4.5 with acetic acid), detecting wavelength at 254nm and a flow rate of 1.0 mL/min at room temperature. [Results] Strychnine has a good linearity in the range of 0.031- 2.000?g?mL-1. The minimum detecting concentration was 0.01?g?mL-1 and the average recovery was over 90%. Intra-day RSD and inter-day RSD ranged from 1.53% to 5.32%. [Conclusion] This method is simple and efficient, and can be used for the determination of strychnine.

Objective To investigate the value of SPECT imaging in the evaluation of rCBF improvement after bypass surgery in patients with moyamoya disease (MMD).Methods Retrospective analysis was conducted in 13 patients with MMD (5 children aged 3-8 years and 8 adults aged 45-55 years).The SPECT cerebral perfusion imaging data before and after operation were analyzed.Age matched subjects who underwent cerebral perfusion imaging for accidental headache (10 children,10 adults) were taken as control group.Two-sample t test or paired t test was used to compare rCBF of different groups.The brain DSA and MRI results during follow-up were taken as the reference of perfusion improvement.Results Based on visual judgment of SPECT,11/13 in MMD group showed significant improvement in the blood perfusion reduction area after surgery.The coincidence rates of SPECT-DSA and SPECT-MRI was 8/11 and 9/11,respectively.The rCBF of the control group and MMD group before surgery was (51.19±1.63) ml · 100 g-1 · min-1 and (38.83±2.22) ml · 100 g-1 · min-1 respectively (t=-18.465,P＜0.05).The rCBF of MMD group before and after surgery was significantly different ((38.83±2.22) ml · 100 g-1 · min-1 vs (45.03±2.63) ml · 100 g-1 · min-1,t=-11.707,P＜0.05).The rCBF of MMD group after surgery and the control group was significantly different (t =-8.313,P＜0.05).There was no significant difference of rCBF between the children and adults in the control group ((51.81±1.82) ml · 100 g-1 · min-1 vs (50.55±1.18) ml · 100 g-1 · min-1 ;t=0.081,P＞0.05).Conclusions Brain perfusion SPECT may effectively reflect the regional distribution and changes of blood flow in the ischemic tissue before and after MMD surgery.Thus it may be helpful in evaluation of the effect of surgical treatment.

Objective To provide a basis for further exploration of Dunaliella Salina by determining the main active components in the extract of Dunaliella salina. Methods The carotenoids and vitamins were determined by HPLC or colorimetric method, amino acids were analyzed by amino acid analyze. Unsaturated fatty acid was determined by GC. Atomic absorption spectrophotometry was used to detect K, Na ,Ca, Mg, Cu, Zn, P, Fe, Mn and SPF was used to detect Se. Results There were affluent carotenoids, vitamins, amino acids, unsaturated fatty acid and trace elements in the extract of Dunaliella salina. Conclusion The extract of Dunaliella salina could be used as natural raw material to develop the supplement food.

After thelibrary cloudwas established using thecloud computing-related,virtualization-relat-ed and special communication channel-related techniques , the abundant resources in library were sent to the health institutions, officers and men in the grass-rooted military units through the special virtual network, thus the information resources-sharing was realized in hospitals of station troops and grass-rooted military units.

[Objective] To establish a method for the determination of brucine and strychnine contents in Tongbiling Tablet. [Methods] Reversed-phase HPLC conditions were: Nucleodur C18 Gravity column (125 mm ? 4.6 mm, 5?m), mobile phrase being methanol-water (60:40) with 0.5 g potassium dihydrogen phosphate and 1.0 g dodecyl sodium sulfouate and 0.5 mL triethylamine in 1 000 mL mixture (adjusting pH to 4.5 with acetic acid) , detecting wavelength at 254 run and a flow rate of 1.0 mL/min at room temperature. [ Results ] The average recovery of brucine was 97.69 % ( RSD = 2.03%) and that of strychnine 97.93% ( RSD = 2.61%). [Conclusion] This method is efficient and can be used for the quality control of Tongbiling Tablet.

Objective To investigate the effect of Diane-35 and metformin on blood-adipose and sex hor-mone in patients with polycystic ovary syndrome ( PCOS ) .Methods 52 cases with PCOS admitted from Jan . 2010 to Jan.2013 were selected as research objects .They were randomly divided into the control group and the observation group .The control group were treated with metformin , while the observation group with metformin and Diane-35.Follicle-stimulating hormone ( FSH), luteinizing hormone ( LH), testosterone ( T), total cholesterol (TC), triglyceride(TG), high density lipoprotein(HDL), low-density lipoprotein(LDL)in the 2 groups of pa-tients were tested before and after treatment .Results The levels of blood-adipose and sex hormone between the 2 groups had no statistical difference (P>0.05).The level of T reduced in the control group after treatment , and there was statistical difference compared with that before treatment ( P <0.05 ) .FSH and LH levels did not change obviously in the observation group .Levels of LH and T in the observation group reduced , and had statisti-cal difference with that before treatment (P<0.05).FSH levels did not change significantly after treatment .Lev-els of LH and T had statistical difference between the 2 groups(P<0.05).Levels of TC, TG, and LDL reduced after treatment and had statistical difference compared with those before treatment ( P<0.05 ) while HDL signifi-cantly increased after treatment(P<0.05).Levels of TC, TG, HDL, and LDL between the 2 groups were signif-icantly different(P<0.05).Conclusion Metformin combined with Diane-35 are effective in improving the lev-els of blood-adipose and sex hormone in patients with PCOS .

The clinical features,laboratory data,and therapeutic efficacy of two patients with thyroid hormone resistance syndrome(THRS)were reported.Two cases manifested growth retardation,raised serum TT_3,FT_3levels,and decreased~(131)I uptake.One patient presented with bird face deformity,nerve deafness,and raised TSH level.The superphysiological dosage of exogenous thyroxin inhibited the synthesis and release of thyrotropin.Thyroxin treatment proved to be effective for the patients.THRS is a disease related to thyroid hormone receptor gene mutation.The final diagnosis of this disease depends on gene analysis.

Objective To explore and evaluate the effect of 32P and pinyangmycin injected in combination for the treatment of maxillofacial hemangioma. Methods The patients were randomly divided into three groups. The first group of patients was treated with 32P; 100 patients were enrolled in this group, of which 37 were male and 63 female. 32P dosage between 0.37 and 0. 74 MBq per cm3 was prepared together with 0.5 ml of 2 %lidocaine, and appropriate normal saline, and then the mixture was injected into hemangioma every two weeks. The second group was given pinyangmycin, and the third group received both 32P and pinyangmycin. The second group included 30 male and 60 female, and the third group 64 male and 136 female. The dose and frequency were given as the same to the first group. Results 88.5 % of patients were cured using both 32P and pinyangmycin, and the cure rate was 77.0 % and 71.7 % with either 32P or pinyangmycin. Conclusion These results prove that 32P and pinyangmycin injected in combination for the treatment of maxillofacial hemangioma is more effective than either 32P or pinyangmycin alone, and furthermore, the method is easy, safe and has less complications.

Objective To establish the fingerprint chromatogram of Buzhong Yiqi Decoction(BYD) by multiple wavelength method,and to analyze the substance of formulas as a whole.Methods HPLC-PDA method was adopted.The chromatographic conditions were as follows:Hypersil ODS2 analytical column,the mobile phase consisting of 0.05 %phosphate acid and acetonitrile with gradient elution,detecting wavelength at 254 and 280 nm,the flow rate being 1.0 mL/min and the temperature of the column at 30 ℃.Results The methodological results were good.The results of dual-wavelength fingerprint chromatogram showed the all-around information of the fingerprints at 254 and 280 nm.The similarity of 10 batches of BuzhongYiqi Decoction was over 0.98.Conclusion Dual-wavelength fingerprint chromatogram can realize the analysis of a formulas as a whole,which supplies references for improving the quality control of BuzhongYiqi Decoction.