The effects of ?-carotene from Dunaliella salina(?-C) at three doses(12.5,25 and 50 mg?kg~(-1),ip) on learning and memory in rats and mice were studied by using step-down test and Y-maze test.In step-down tests,?-C at all three tested doses had significant effects on normal mice,and could remarkably antagonize the memory impairment induced by scopolamine and 20 % alcohol,but could not antagonize the impairment induced by sodium nitrite.As the same result,?-C at three tested doses administration had significant effects on rats in Y-maze tests.These results suggested that ?-C as an antioxidant could improve the ability of learning and memory in rats and mice.

Objective: To explore evaluation effect of three-dimensional speckle tracking imaging (3D-STI) on left ventricular systolic function in patients with acute anterior myocardial infarction (AAMI).Methods: A total of 40 AAMI patients and 40 healthy subjects were enrolled as AAMI group and healthy control group respectively.Left ventricular longitudinal strain (LS), radial strain (RS), circumferential strain (CS), area of strain (AS), general longitudinal strain (GLS), general radial strain (GRS), general circumferential strain (GCS) and general area of strain (GAS) were measured, compared and analyzed between two groups for 3D-STI technique.Results: Compared with healthy control group, there were significant reductions in left ventricular LS of 12/17 segment, RS and AS of 10/17 segment, and CS of 9/17 segment in AAMI group, P<0.05 or <0.01;for general strain values, compared with healthy control group, there were significant reductions in GLS[(19.59±3.52) vs.(9.25±2.16)], GRS[(36.73±4.83) vs.(25.26±5.19)], GCS[(22.63±4.38) vs.(15.15±3.98)], GAS[(32.87±4.41) vs.(21.73±3.84)]in AAMI group, P<0.01 all.Conclusion: The three-dimensional speckle tracking imaging can accurately detect segmental myocardial strains of left ventricular systolic function in patients with acute anterior myocardial infarction.It can be used as a quantitative measure for clinical evaluation of these patients′ condition.

OBJECTIVE:To evaluate the bioequivalence of compound fluamine tablets with commercial fluamine tablets and glibenclamide tablets(R).METHODS:HPLC method was used to determine the plasma drug time-concentrations of flu?amine and glibenclamide separately.The pharmacokinetic parameters were calculated and analysed with variance and two-one side t-test.RESULTS:The T 1/2 ,T max ,C max and AUC 0～24 for fluamine of test and reference formulation were(7.969?0.713)h and(8.103?0.596)h;(1.889?0.214)h and(1.876?0.207)h;(2.001?0.368)?g/ml and(1.962?0.416)?g/ml;(10.706?1.424)(?g?h)/ml and(10.624?1.317)(?g?h)/ml respectively.F was(100.97?7.73)%.The T 1/2 ,T max ,and AUC 0～24 for glibenclamide of test and reference formulation were(6.536?0.890)h and(6.629?0.563)h;(4.038?0.309)h and(4.056?0.338)h;(98.026?22.790)ng/ml and(97.507?20.529)ng/ml;(510.702?135.818)(ng?h)/ml and(499.452?111.328)(ng?h)/ml,respectively,and F was(102.066?12.405)%.CONCLUSION:The statistical analysis showed that com?pound fluamine tablets and commercial fluamine tablets and glibenclamide tablets were bioequivalent.

0.05).In the combined procedure group,no short-term bleeding occurred and the incidence of rebleeding was 5.7% during(long-term) follow-up.The incidence of rebleeding was significantly lower in the combined procedure group than in EVL and Hassab operation group(P

Objective:To provide the references for the dieting of sportsman.Methods:divided 24 mice into three groups-still group,training group,and medicine-taking group.Then the blood was draun from eye socket to test ALT,AST.LDH and BUN,Glue and Hb value through the out-running experiment.Results:The mice in the medicine-taking group appear to last the sporting ability,with the improved time of 24.43%,and the change of hepatin,blood sugar and testosterone were less than thasein the other two groups.Conclusion:Excessive movement can lead to the level of testosterone down,while the distilled things from burdock can help sportsman's testosterone up,then to increase the storage of hepatin and the ability of sporting.

Objective To discuss the application of ice-breaking action in the construction of nurse team, strengthen the cohesion of nurse team, therefore, to improve nursing quality. Methods First, let all nurses working in the same ward area to participate an action, namely ice-breaking action or secret angel action, in which every nurse chose secretly one of other nurses by drawing cards, then she often cared for that nurse secretly, namely she became a secret angel for that nurse. In return, she was also chosen and cared by one of others secretly. That means every nurse would become a secret angel, and owned a secret angel in the same time. Second, to test the change of happiness index of every nurse, the change of team's cohesion, and the change of patients' contentment indexes, before and after the secret-angel action was taken. Results Happiness indexes of 37 participants improved distinctly, and so did the contentment indexes of their patients after the secret-angel action was taken. Conclusions The secret-angel action can strengthen the cohesion of nurse team, improve happiness indexes of nurses, contentment indexes of their patients, and improve nursing quality.

OBJECTIVE:To establish a HPLC method for determination of paeonol and imperatorin in Cangzhi nose spray. METHODS:HPLC system consisted of ODS C 18 column(150mm?4.6mm,5?m)methanol-water(6∶4)mixture as mobile phase,with detection wavelength at310nm,flow rate1.0ml/min.RESULTS:The average recoveries of paeonol and imperatorin were99.4%(RSD=0.69%)and99.9%(RSD=2.66%)respectively.CONCLUSION:The method is sensitive,rapid and ac?curate.

P-glycoprotein is one of the members in the superfamily of ATP-binding cassette transporters. It is expressed in many sites in vivo, and is correlated with multidrug resistance. Under physiological conditions, as an efflux pump, P-glycoprotein in the blood-brain barrier can eliminate endogenous substrates and xenobiotics to maintain the balance of internal environment. But at the same time, it also limits the concentration of therapeutic drugs in brain, and thus reduces therapeutic efficacy. P-glycoprotein inhibitors can get drugs across the blood-brain barrier. It is of great importance to improve the blood concentration in brain and bioavailability of central nervous system drugs.

[ Objective ] To establish a method for the determination of serum strychnine concentration in rheumatoid arthritis patients treated with Tongbiling prescription and to monitor the serum strychnine concentration. [ Methods ] Serum strychnine was extracted with solid phase extraction column. Reversed-phase HPLC conditions were: Hypersil Division ODS C18 column (150 mm ?4.6 mm, 5?m), mobile phrase being methanol: water (70 : 30) with 0.6 g potassium dihydrogen phosphate and 1.0 g dodecyl sodium sulfonate added in 1000 mL mixture (adjust pH to 4.5 with acetic acid), detecting wavelength at 254nm and a flow rate of 1.0 mL/min at room temperature. [Results] Strychnine has a good linearity in the range of 0.031- 2.000?g?mL-1. The minimum detecting concentration was 0.01?g?mL-1 and the average recovery was over 90%. Intra-day RSD and inter-day RSD ranged from 1.53% to 5.32%. [Conclusion] This method is simple and efficient, and can be used for the determination of strychnine.

Objective To study the expressions of Connexin43 (Cx43) and B-cell lymphoma/leukemia-2 (Bcl-2) proteins in the ventricular myocardium of human fetus,and explore the correlation between the expression of the two proteins and the development of fetus myocardium.MethodsThe expressions of Cx43 and Bcl-2 protein in the ventricular myocardium of human embryos were immunohistochemically detected in the second,third and fourth month of gestation,respectively,by strepto-avidin-biotin-peroxidase complex (SABC) and avidin-biotin-peroxidase complex (ABC) technics.ResultsThe positive expression of Bcl-2 protein was detected in one or two cells in the ventricular myocardium of human embryos in the second month of gestation.With the advancement of fetal age,the number of Bcl-2 positive cells increased gradually and distributed in clusters in the ventricular myocardium of human fetus in the fourth month of gestation.The cells with positive expression of Cx43 protein were detected and scattered in dots-like structure in the cytoplasm of myocardial cells in the ventricular myocardium of human embryos in the second month of gestation.With the advancement of fetal age,the distributing region of Cx43 protein extended gradually,and the Cx43 protein positively expressed in the membranes between ventricular myocardial cells.The positively expressed Cx43 protein showed a diffused distribution in a sheet form in the ventricular wall of human fetus in the fourth month of gestation.ConclusionCx43 and Bcl-2 proteins participate in the early growth and development of the ventricular myocardial cells of human fetus.