Objective To find out an effective therapy to treat pollinosis in Switzerland by Chinese herbs and acupuncture & moxibustion.Methods Gu Beng Tuo Min Fang and Acupuncture & moxibustion were used on 68 patients with pollinosis,and compared with clinical efficacy before and after treatment.One course was 20 times,and Continued about 3 months.Results Among the treated 68 cases,cured in 47,improved in 12,and total effective rate was 86.8%.Conclusion A combination of Chinese herbs and Acupuncture & Moxibustion has an exact therapeutic effect on pollinosis in Switzerland.

Objective To investigate the expression of extracellular signal regulated kinase(ERK) in human gastric cancer and evaluate the relationship between ERKs protein level and clinicopathological parameters. Method Western blot was used to measure the activity and expression of ERK 1 and ERK 2 proteins and immunohistochemistry was used for localization in 42 gastric cancer patients.Results ERK 1 and ERK 2 protein levels increased in gastric cancer tissues in comparison to normal tissues ( P 0 05). Expression of ERK 1 and ERK 2 showed a positive linear correlation (r=0 550, P

ObjectiveTo investigate p38 MAPK protein expression in human breast tumors and the regulation of preoperative chemotherapy. MethodsWe assessed p38 MAPK protein in 50 cases of breast cancer and adjacent normal tissue and that of breast benign tumors in 14 cases by Western blot. 15 cancer patients received preoperative CAF or TA protocol. Results The p38 protein level in tumors was significantly greater than that in the normal tissues, while there was not significant difference between benign tumors and corresponding normal tissue. p38 protein T/N ratio was higher in patients with lymph node metastasis than patients without( P

Objective: To evaluate the clinical efficacy and safety of combining acanthopanax senticosus and lithium carbonate for the treatment of adolescent depression. Methods: 65 outpatients or inpatients were enrolled in a double-blind randomized clinical study which was conducted comparing combining acanthopanax senticosus and lithium versus combining fluoxetine and lithium for 6 weeks. Efficacy and side effects were assessed with Hamilton depression rating scale (HAMD) and treatment emergent symptom scale (TESS) respectively before and after the treatment. Results: 63 patients finished the trial, the rate of dropout was 3.08%. Altogether, 65 patients were enrolled in the study, only 32 were enrolled in ITT analysis and PP analysis in acanthopanax senticosus group, while 33 were enrolled in ITT analysis and 31 were eligible for PP analysis in fluoxetine group. The HAMD scores were significantly decreased after treatment at each weekly point in both groups. The difference of decreased HAMD scores between the two groups was not significant in weeks after treatment. At the end of treatment, the rate of utility and significant utility had no significant difference between the two groups. There were two patients who developed mania, while the rate of mania-conversion had no significant difference. Conclusion: Combined acanthopanax senticosus and lithium carbonate is an effect therapeutic scheme to adolescent bipolar depression.

Objective:To optimize the matrix formula of Qingre Zhitong cataplasm. Methods:An orthogonal test was performed to study the matrix formula of Qingre Zhitong cataplasm with the comprehensive score of continued adhesion and integrated sensory as the evaluation indices and the ratio of sodium polyacrylate, carboxymethylcellulose sodium, aluminum chloride hydrate and glycerin as the influencing factor. Results:The optimal matrix formula of Qingre Zhitong cataplasm was as follows: the ratio of sodium polyacrylate, carboxymethylcellulose sodium, aluminum chloride hydrate and glycerin was 14 ∶1 ∶1 ∶120. Conclusion: Qingre Zhitong cataplasm prepared with the optimal matrix formula has good continued adhesion and integrated sensory, and the process parameters are stable and feasible.

The proliferation of Patu8988 cells pre-treated with metformin for 72h was measured by CCK-8 assay.Cell cycle was analyzed by flow cytometry.The expression of related genes was detected by RT-PCR.Metformin suppressed the proliferation of Patu8988 cells in a dose-dependent manner( r=0.994,P＜0.05 ).The proportion of cells at G0/G1 stage was increased while that of G2/M stage decreased (P＜0.05 ).The expressions of MMP-3,cyclinD1,p53 were down regulated and that of Bax was up regulated.The results show that metformin may inhibit the proliferation of Patu8988 cells,via blocking the cell cycle and affecting the expression of related genes.

Aim To prepare the monoclonal antibodies (mAbs) against human CD28 and to study its biological feature. Methods The hybridoma cell lines were obtained by fusing spleen cells of Blab/c mice that had been immunized with murine lymphoma cells transfected with full-length huaman CD28 cDNA to myeloma cells Sp2/0. Ascites were induced to produce the mAbs. The specificity and affinity of the mAb 18G8 was verified by CD28 competitive inhibitory test and FACS. Reactivities of mAb 18G8 to PBTC, U266, 8226, Jurkat and Daudi cell were studied by indirect immunofluorescence staining. mAb 18G8-inducing proliferation of peripheral blood T cells (PBTCs) was determined by [3H]thymidine incorporation test. Results Five hybridoma cell lines were obtained. mAb 18G8 secreted by one of the them, belong to mouse IgG2a. It recognized a epitope different from which recognized by the standard mAb(clone CD28.2). The Reactivitrates of the mAb 18G8 to PBTC, U266, 8266, Jurkat and Daudi cells were 70.2％ , 99.3％ , 98.6％ , 76.4％ and 1.9％ , respectively, similar with CD28.2. It was indicated that different antigen epitopes expressed on all above cells. mAb 18G8 could promote the PBTC proliferation in vitro(SI=7). It was indicated that The substitution of mAb 18G8 for B7-1 molecule could also mediate the costimulatory signals. Conclusion 18G8 is a specific and functional anti-CD28 mAb it may be of significant value in basic studies and clinical application.

Objective To explore the expression of membrane form of CD28 (mCD28) on T lymphoeytes and the serum level of soluble CD28 (sCD28) in elderly patients with primary non-small cell lung cancer (NSCLC) in order to investigate the relationship between age-related changes of CD28 and the development of NSCLC in elderly patients. Methods 63 elderly patients with NSCLC, 35 elderly patients with lung benign lesion, 30 elderly healthy donors, 30 young healthy donors, 20 young patients with lung benign lesion and 20 young patients with NSCLC were enrolled in this study. The mCD28 on T cells and the serum level of sCD28 were measured by four-color flow eytometric assay and enzyme linked immunosorbent respectively, and the relationship between CD28 and clinical characteristics of NSCLC was analysed Results The expression of mCD28 was decreased and the serum level of sCD28 was increased in elderly patients with NSCLC compared with the other groups (F= 184.25, P<0. 01 ; F= 365.40, P<0.01). The expression of mCD28 was significantly lower and the level of sCD28 was significantly higher in elderly healthy donors than those in young healthy donors and young patients with lung benign lesion (P<0. 05). There were no significantly statistical differences in expression of mCD28 and level of sCD28 between elderly healthy donors and elderly patients with lung benign lesion [(42.84±5.82)% vs. (46.09±-7.34)%, (39.38±6.02)μg/L vs. (35.84±5.02)μg/L, P>0. 05]. Logistic regression analysis showed that aging (OR=2. 432), down-regulation of mCD28 expression (OR=0. 876) and up-regulation of sCD28 level (OR= 1. 113) were the risk factors for lung cancer. In the elderly patients with NSCLC, there were significant differences in mCD28 expression and sCD28 level between stages Ⅲ-Ⅳ and stages Ⅰ-Ⅱ [(16. 51± 5.64)% vs. (24.41±8.24)%, (75.03±5.98) μg/L vs. (66.73±7.52)μg/L; t=4.497,4.794, both P <0. 01]. However, there were no significantly statistical differences among different pathological types (F=0. 609, 0. 302, both P > 0. 05). Conclusions The down-regulation of mCD28 expression and up-regulation of sCD28 level with advancing age play an important role in the oncogenesis and development of primary non-small cell lung cancer in the elderly patients.

BACKGROUND:Previous studies have mainly focused on costal cartilage, articular cartilage, nasal septal cartilage, and auricular cartilage, but in vitro culture of human vertebral endplate cartilage is stil rarely reported. OBJECTIVE: To discuss the feasibility of culture of vertebral endplate chondrocytes from type I neurofibromatosis associated with scoliosis patientsin vitro and to study the biological characters of the chondrocytes. METHODS: Through two-step enzymatic digestion and tissue culture, the chondrocytes from the vertebral endplate of seven type I neurofibromatosis patients isolated and cultured in monolayer and passaged to observe the changes of cel morphology under inverted phase contrast microscope. Colagen type II expression was detected by immunocytochemistry to identify whether the cels had chondrocyte characters. The growth kinetics was detected by using MTT colorimetric assay to draw the growth curve of passage 2 chondrocytes. RESULTS AND CONCLUSION:A few chondrocytes crawled from the cartilage after 2 weeks culture and cels were passaged at 3 weeks. Along with passage going on, the phenotype of chondrocytes was changed from polygonal, round, triangle, and irregular shapes to fusiform. The colagen type II expression in passage 2 cels was positive by immunohistochemical staining. MTT test showed the growth curve of the passage 2 chondrocytes presented a transverse “S”. Cels were found logarithmic growth at days 4-7, reached platform stage at days 8-13, and decreased at day 14. It is an effective and simple procedure by two-step enzymatic digestion and tissue explant method to culture vertebral endplate chondrocytes with high purity and good viability from type I neurofibromatosis patients associated with scoliosisin vitro. Passage 2 chondrocytes from the vertebral endplate exhibit the best viability at days 4-7, which can be used as targets for research of pathogenesis of type I neurofibromatosis with atrophic scoliosis.

1000 ml).Methods From 2000 to 2002,a total of 89 critically ill surgical patients were enrolled whose preoperative liver function and blood analysis were normal. The patients′ heart rate?blood pressure?body temperature?hemoglobin(Hb)?platelet count(PLT)?hematocrit(HCT)?prothrombin time(PT)?activated partial thrombo-plastin time(APTT)?fibrinogen concentration(FIB)?fibrinogen degrade product(FDP)?D-dimer immediately after enrollment into the study were determined. Results As the blood loss increased PT prolonged from(11.4?0.6) s to (16.0?0.6) s ( P