Objective:To analyse the therapic effect of low-voltage electrochemotherapy (EP) on the head and neck cancer.Method:YCU-N861 human head and neck cancer cells were subcutaneously inoculated in the neck of nude mice to establish tumor-bearing models. Then the mice were treated with bleomycin(BLM, 20 μg per mouse), accompanied with electric stimulation(50 V or 100 V).Result:BLM treatment applied with EP can significantly inhibite the proliferation of tumor cells than BLM or EP treatment seperately(P<0.01) . The tumor volume, the mitotic index (MI) and the labeling index (LI) of Ki67 in BLM+EP group were all obviously decreased than BLM group, EP group and control group (P<0.01).Conclusion:Low-voltage electrochemotherapy can offer promising mothod in the local treatment of head and neck cancer.

we reviewed data from 547EEGs on 400 patients with epilepsy in order to determine the probability in finding the interictal epileptiform activity (IIEA) with serial EEGs. In 49.5% of our patients, the abnormaeity is present on the first record. In 53.5% by the second EEG, and in 54.25% by the third or above. There is relatively little yield to serial EEGs beyond this point. Patient's ages, types of seizures and causes are negatively correlated to IIEA yield

The genetic diagnosis of hemophilia is a basic method for preventing the birth of hemophilia patients and a basic way for realizing the aim of aristogenesis.Direct and indirect genetic diagnosis is a basic strategy for screening of hemophilia;while in indirect genetic diagnosis,more attention should be paid on the information given by the intro-genetic sites combined with the extra-genetic sites.

Objective To clone the full-length cDNA of rat B cell lymphoma-2(bcl-2) gene,then construct and identify the cytomegavirus-mediated lentiviral expression vector of bcl-2 gene,and assess the gene expression in 293T cell,which is a human embryonic kidney cell line.Methods The full-length bcl-2-cDNA fragment was amplified by reverse transcription polymerase chain reaction(RT-PCR) from the kidney tissue of a Wistar rat.The double-stranded oligonucleotides(dsOligoe) were then cloned into the pMD18-T plasmid.After confirmation of a correct construction by sequencing,the positive clone was subcloned into pGC-FU vector with enhanced green fluorescent protein(EGFP),and then transformed into DH5a competent cells.The restricted endonuclease and T4 DNA ligase were used to construct the lentiviral expression vector plasmid pGC-FU-bcl-2 which,combined with the lentiviral packing materials(pHelper 1.0,pHelper 2.0),was then transfected into 293T cell line to form the recombinant lentivirus pGC-FU-bcl-2,and it was used to transfect the 293T cells.The expression of pGC-FU-bcl-2 was further verified by detecting EGFP and bcl-2.Results 1) It was verified by DNA sequencing that the sequence of rat bcl-2 gene was consistent with reported sequence in GenBank.2) The bcl-2 gene was successfully combined in pGC-FU-bcl-2 recombinant plasmid which could be transfected into human embryonic kidney cells.3) The recombinant virus pGC-FU-bcl-2 could be obtained from the 293T cells by co-transfection of pGC-FU-bcl-2 and packing plasmids.4) Targeting gene could be cloned into 293T cells by the recombinant lentivirus with steady expression.The fluorescent protein could be observed under microscope and the expression of bcl-2 protein was detected by Western blotting.Conclusions The lentiviral expression vector containing EGFP and bcl-2 gene has been successfully constructed,with which the transfected 293 T cells can lead to a steady expression of bcl-2 protein.The present study provides a basis for the further study of the function of bcl-2 gene and a potential therapy for the diseases relating to apoptosis.

Colonoscopy has become the most commonly used method to the diagnosis and treatment of colorectal diseases at present.Colonoscopic perforation is considered the most serious complication with high mortality, especially in the patients with certain risk factors and iatrogenic factors, despite of its low incidence.According to the clinical features in the period of perioperative colonoscopy, early detection of colonoscopic perforation is a prerequisite for successful treatment and the key to lower mortality.So far in the treatment,some methods can be selected, including conservative treatment, open surgery, laparoscopic surgery and endoscopy folders, etc.Clinical judgement and analysis of disease should be specific and accurate, but not to be generalized.Doctors should adopt reasonable and effective treatment options according to clinical performance, the size of perforation,intestinal readiness, time of diagnosis, colon primary lesion and so on.

Objective To investigate the sedative effects of different fractions of patrinia scabiosaefolia fisch. in mice and provide some valuable information for isolating components with sedative effect.Methods Petroleum, acetic ether and n-butanol fraction was isolated from the patrinia scabiosaefolia fisch.. The sedative effects were determined with locomotor activity assay, hole board test and sodium thiopental-induced sleeping time assay, and it was compared with the effects of total 70% ethanol extract. Results N-butanol fraction showed better sedative effect compared with others, and it was in a dose-dependent manner. Conclusion The results showed that the main active principles of patrinia scabiosaefolia fisch. were the n-butanol fraction, and the promising active chemical might be obtained from this fraction.

BACKGROUND: Excessive apoptosis of ovary granulosa cell is a dominant cause of premature ovarian failure and bcl-2 gene is able to inhibit cell apoptosis. But studies demonstrate that,the guanine and cytosine (GC) content reaches 60% in the rat bcl-2 gene sequence. This gene cannot be amplified using routine polymerase chain reaction method. OBJECTIVE: To clone and identify the bcl-2 gene riched GC. DESIGN,TIME AND SETTING: Open experiment was finished in the Laboratory of Medicine and Molecular Biology,Life Science School of Sun Yat-sen University from May to December in 2007. MATERIALS: Wistar rats were purchased from Experimental Animal Center of Sun Yat-sen University. Ecoli DH5?was preserved by Laboratory of Medicine and Molecular Biology,Life Science School of Sun Yat-sen University; pMD18-T vector was purchased from Takara Biotechnology (Dalian) Co.,Ltd. METHODS: The bcl-2-cDNA,in which GC accounted for 60.6%,was obtained by modified reverse transcription-polymerase chain reaction from kidney tissue of Wistar rats,and was cloned into vector-pMD18-T. Characterizations and sequencing of the pMD18-T-bcl-2 were carried out by polymerase chain reaction screening of individual bacterial colonies. MAIN OUTCOME MEASURES: Cloning and purification of bcl-2 gene cDNA; results after connecting bcl-2 gene cDNA to pMD18-T vector and transducting Ecoli DH5?,identification of positive clone and results of sequencing. RESULTS: The bcl-2 gene was identified by the clone and DNA sequencing. DNA sequence analysis was consistent with Genebank sequence,with a 99% homology. CONCLUSION: The gene riched GC is difficult to be amplified,bcl-2-cDNA can be cloned and constructed into cloning vector pMD18-T successfully by the efficient technique for other genes riched GC.

OBJECTIVE To investigate the clinical and bacterial efficacy and safety of cefoperazone/sulbactam(Sulperazone) on severe nosocomial lower respiratory infections. METHODS The clinical materials in the treatment of severe nosocomical lower respiratory infections from Mar 2002 to Mar 2005 were analyzed retrospectively. RESULTS The overall clinical efficacy rate of Sulperazone was 83.9%,the bacterial eradication rate was 92.%,The sensitivity rate to Sulperazone was 88.2%.There was no adverse reaction in the observation. CONCLUSIONS Sulperazone is effective and safe in the treatment of severe nosocomial lower respiratory infection.

Objective To investigate the clinical efficacy of amoxicillin combined with pectin in the treatment of chronic atrophic gastritis. Methods According to the different treatment methods will be January 2015 to March 2017 patients with chronic atrophic gastritis in our hospital 80 cases groups:control group with clarithromycin+amoxicillin+bismuth pectin bismuth pectin treatment,observation group treatment;detailed records of two groups before and after treatment(pathological atrophy,intestinal metaplasia,dysplasia,chronic the inflammatory activity and the integral changes),the levels of inflammatory factors(IL-2/IL-6/CRP)incidence level changes and adverse reaction,the obtained related data for comparative analysis. Results Amoxicillin and bismuth pectin(observation group)in treatment of chronic atrophic gastritis is better than clarithromycin+bismuth pectin(control group)treatment,patients with pathological score decreased than the control group,the levels of inflammatory factors is better than the control group,the incidence rate of adverse reaction was lower than the control group,the difference was statistically significant(P<0.05). Conclusion Chronic atrophic gastritis patients choose amoxicillin and bismuth pectin treatment effect significantly,can greatly reduce the pathological integral,improve clinical symptoms,and can reduce the level of inflammatory factors in patients with a low incidence of adverse reactions,it is worthy of widely used to treat patients with chronic.

Objective To analyze the curative effect of double path endoscopic surgery in the treatment of fungal sinusitis,and to compare with the effect of simple in endoscopic nasal passages windowing.Methods The clinical data of fungal sinusitis patients were analyzed.According to the different surgery way,the patients were divid-ed into two groups.The observation group:nasal endoscopic nasal passages open a window in the joint of fangs nest small windows double path;Control group:simple in nasal endoscopic fenestration.Results The observation group had 35 cases and the control group had 37 cases.After treatment,the symptoms score of the observation group was sig-nificantly lower than the control group [(1 .09 ±0.83)points vs (1 .47 ±0.77)points;t =2.01 5,P =0.048].The effective rate of the observation group was significantly higher than the control group (94.29% vs 75.68%;χ2 =4.81 3,P =0.028).7 days after treatment,the C -reactive protein(t =4.971 ,P <0.01 ),TNF alpha(t =9.1 9,P <0.001 ),beta IL -1 (t =5.635,P <0.01 ),white blood cells (t =3.25,P =0.002)and IL -6(t =3.86,P =0.002) in the observation group were significantly lower than the control group (P <0.05).The preoperative plasma level of 1 -3 -β-glucan D between the two groups had no statistical difference (P >0.05).2(t =5.978,P <0.01 ),4(t =6.335,P <0.01 ),6(t =9.348,P <0.01 ),and 8 days(t =8.226,P <0.01 )postoperatively,1 -3 -β-glucan D levels were significantly lower than the control group,the differences were statistically significant (P <0.05).The postoperative recurrence rate of the observation group was significantly lower than the control group (8.57% vs 27.03%,the Log -rank χ2 =4.061 ,P =0.044).Conclusion In fungal sinusitis treatment,the use of endoscopic nasal passages open window joint fangs nest small windows double path can more effectively remove lesions,its forward curative effect is superior to pure in nasal endoscopic fenestration.