Objective To observe the effect of heat-producing needling (Shao Shan Huo) and cool-inducing needling (Tou Tian Liang) on the gait cycle after operation for gluteus contracture. Method Sixty-six patients after operation for gluteus contracture were randomized into a treatment group and a control group, 33 in each group. The control group was intervened by rehabilitation, while the treatment group was by acupuncture in addition to the rehabilitation training. The topical points Zhibian (BL 54), Huantiao (GB 30), and Juliao (GB 29) were treated with reducing cool-inducing needling technique; Biguan (ST 31), Futu (ST 32), Zusanli (ST 36), Fenglong (ST 40), Xuehai (SP 10), Diji (SP 8), and Yanglingquan (GB 34) were treated with reinforcing heat-producing needling technique. The motor time parameters were observed before and after intervention.Result After intervention, the supporting phase of the affected side, supporting phase of the healthy side, gait cycle, swinging phase of the affected side, swinging phase of the healthy side, and bilateral supporting phase of the treatment group were significantly different from that of the control group (P<0.05).Conclusion Heat-producing and cool-inducing needling techniques can effectively shorten the gait cycle after operation for gluteus contracture, and can improve the walk function.

Objective To investigate the prevalence and influencing factors in senile cataract in Huzhou city.Methods 1 680 cases of healthy physical examination elderly population were selected as the research subjects in Huzhou city.To investigate the prevalence of cataract in the elderly population.The correlation of the prevalence of senile cataract,visual impairment and sex,age was studied.The correlation of the prevalence of senile cataract and diabetes,hypertension,smoking was analyzed.At the same time,Logistic regression analysis was used to analyze the influence factors of senile cataract.Results In 1 680 cases of elderly people,299 cases of cataract,the prevalence rate of cataract was 17.79%.With age increased,the prevalence of senile cataract was gradually increased.The preva-lence of cataract in female was 21.80%,which was higher than 15.82% in male,and the difference was statistically significant (χ2 =9.083,P <0.01).The visual acuity of senile cataract patients would increase with age.Moreover, the visual acuity of female patients was higher than that of male patients.The prevalence of cataract in diabetic group was 64.58%,which was higher than 17.89% in non -diabetes group,the difference was statistically significant (χ2 =65.457,P <0.01 ).The prevalence of cataract in hypertension group was 61.48%,which was higher than 14.31% in non -hypertension group,and the difference was statistically significant (χ2 =163.538,P <0.01).The prevalence of cataract in smoking group was 80.39%,which was higher than 16.94% in non - smoking group,the difference was statistically significant (χ2 =130.046,P <0.01).Logistic regression analysis showed that gender,age, diabetes,hypertension,smoking,and so on were the factors affecting the incidence of cataract in the elderly popula-tion.Conclusion The prevalence of cataract in the elderly population in Huzhou city need to pay attention to the local health department.

AIM:To establish a GC/MS method for determining the fingerprint of Jingu Tongxiao Pills.METHODS:GC/MS condition was comprised of column:DB-1(0.25 mm?30 m,0.25 ?m);injector temperature of 250 ?C;interface temperature of 250 ?C;column temperature of 70?C(1min)4?C/min→130?C(15min)1?C/min→150?C10?C/min→250?C(7min);carrier gas:He;column pressure:60 kPa;ionization souse:EI;detector volts:1.00 kV;mass range:41～350 aum.RESULTS:17 peaks existed on the GC/MS fingerprint of Jingu Tongxiao Pills.CONCLUSION:The method can provide more information for the quality control of Jingu Tongxiao Pills.

Objective To explore the clinicopathological and immunohistochemical features of wolffian adnexal tumour ( WAT) . Meth-ods The clinical and pathological features analysis and immunohistochemistry methods were utilized to study the histopathology features of a case Wolffian adnexal tumor. Results One case of unilateral WAT which located in broad ligament display a solid-cystic mass with pedicled and enveloped,its histology showed large and small tubulars,screen structure and solid zone. Its immunohistochemistry staining was positive for pan-cytokeratin,vimentin,CK18 and CD99,positive staining for α-inhibin in a little tumor cell,and negative for calretinin,CD10,EMA, ER,PR,Syn,CA15-3,CA19-9,Ki-67 index is less than 5%. PAS positive staining for the basement membrane around Gland like structure. Conclusion Wolffian adnexal tumour is diagnosised by its histopathological,immunohistochemical and it’ s distinctive location where Wolffi-an duct remnants are found.

Objective: To observe the clinical efficacy of acupuncture plus patent Chinese medicine in treating post-stroke constipation. ＜br> Methods: Sixty eligible patients with post-stroke constipation were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by acupuncture plusMa Zi Ren pill, while the control group was byMa Zi Ren pill alone. The symptoms of constipation were observed before and after intervention. ＜br> Results: After 2-week treatment, the constipation condition was improved in both groups, and the improvement in the treatment group was statistically more significant than that in the control group (P＜0.05); respectively two weeks and a month after the intervention, the treatment group was superior to the control group in comparing the constipation score (P＜0.05). However, acupuncture didn’t show marked effect in improving defecation speed, initial defecation time, and spontaneous discharge frequency. The treatment group had a significantly higher short-term markedly-effective rate compared with the control group (P＜0.05); the long-term therapeutic efficacy of the treatment group was significantly higher than that of the control group (P＜0.05). The adverse events happened in the treatment group were significantly less than those in the control group (P＜0.05). ＜br> Conclusion: Acupuncture at specific acupoints plus patent Chinese medicine can produce a content therapeutic efficacy.

Objective To study the effect of Zhuang'er Granules(ZG) on cellular immune function of mice.Methods The immunodepression mice models were induced by injection of hydrocortisone.Then the lymphocyte transformation rate was examined by morphological test and phagocyte phagocytosis by in vitro method after 7-day intragastric administration of the drugs.Results Compared with the negative control group,ZG could significantly improve the lymphocyte transformation rate and phagocyte phagocytosis in the normal mice and immunodepression mice(P

Aim To explore the effect of adenylate cyclase(AC) antagonists SQ22536 and agonist forskolin on acute lung injury induced by lipopolysaccharide.Methods ICR mice were randomly divided into normal saline control group(N group), model group(group L), dexamethasone group(group D),AC antagonists s(group SQ) and AC agonist group(group F).The ALI mouse model was induced by instilling intratracheally with LPS(2 mg·kg-1), and 6 h later, the lung tissue and alveolar lavage fluid(BALF) were harvested, pathological changes in lung were observed, white blood cell and neutrophil, albumin content in BALF and myeloperoxidase(MPO) activity of lung tissue homogenate were determined, and tumor necrosis factor α(TNF-α), interleukin-1β(IL-1β), interleukin 6(IL-6) and cAMP content in lung homogenates were detected by ELISA.Results Compared with normal saline group, a large number of neutrophils infiltrated around the pulmonary vessel and airway 6 h after LPS intratracheal instillation in model group.White blood cells and neutrophils and protein content increased in BALF;MPO activity and cAMP levels increased in lung tissues.In the lung tissue TNF-α and IL-6, IL-1β content increased, compared with model group.Forskolin could improve the pathological changes of lung tissue, reduce the total number of leukocytes, number of neutrophils and protein content in BALF, and reduce MPO activity and TNF-α content in lung tissue, at the same time it increased the cAMP content;SQ22536 had no significant effect when compared with model group.Conclusion AC agonists have protective effects on LPS-induced acute lung injury in mice, and the mechanism may be related to elevating cAMP levels, inhibiting neutrophil adhesion and chemotaxis and reducing inflammatory factor levels.

Objective To observe the effect of different doses of butorphanol and sufentanil repeatedly epidural injected on the neurobehavior function in bone cancer model rats.Methods A PE-530 catheter was inserted into the epidural space of all male Sprague-Dawley rats(not mated,weighting 150-180 g) at L1-2 level.Three days after operation,64 rats without any motor dysfunction were randomly divided into eight groups (n=8):sham operated group (group C),normal saline with bone cancer pain group (group N),butorphanol groups(group B 1,B2,B3)and sufentanil groups (group S1,S2,S3).Bone cancer pain model was constructed in group N,B and S when rats in group C were sham operated.Rats in group C and N were epidurally injected NS 30μl each,and rats in group B1,B2 and B3 were respectively epidurally injected butorphanol 25,50,100 μg (all diluted to 30 μl with NS),when rats in group S1,S2 and S3 were respectively cpidurally injected sufentanil 1,2,4 μg (all diluted to 30 μl with NS) on time per day for 10-14 days after modeling.The neurobehavior paw withdrawal threshold (MWT) of the left hind claw was recorded to observe the changes in pain behavior.The neurobehavior function of rats were recorded by BBB (BASSO,BEATTIE and BRESNAHAN) score and the inclined plane test.Results Compared with group C((67.65±9.29) g),the MWT of the model groups obviously decreased before the first time of injection (N (15.23± 2.46) g,B 1 (16.14±2.28) g,B2(15.42±3.22) g,B3(14.35±2.32) g,S1 (15.37±2.11)g,S2(15.22±2.93) g,S3(16.25± 2.36) g) (all P＜0.05)).Compared with group N((16.13±2.37) g),the MWT of group B2,B3 and S3 increased obviously after the first time of injection ((35.12±5.16) g,(35.63± 1.53) g and (34.24±5.93) g) (P＜ 0.05).At the first day of injection,there was no significant difference in the BBB scores and the inclined plane test between the model groups (P＞0.05).At 6 h after the forth injection the inclined plane test and the BBB scores of group B3 were obviously decreased compared with group N ((34.72 ± 4.56) ° and (10.64 ± 1.82) points to (43.15±4.67)° and (14.05±1.78) points (P＜0.05)).Conclusion The results provide evidence that repeatedly epidural injection of butorphanol 50 μg or 100 μg or sufentanil 4 μg can reduce the pain of the rats with bone cancer pain.But repeated epidural injection of butorphanol 100 μg can injure the neurological function.

Objective To analyze the clinical features of AIDS-related Kaposi's sarcoma (AIDSKS) patients in Xinjiang uygur autonomous region,and to evaluate the efficacy of highly active antiretroviral therapy (HAART) and systemic chemotherapy.Methods Clinical data on 70 AIDS-KS patients admitted in Sixth People' s Hospital of Xinjiang Uygur Autonomous Region from January 2008 to June 2013 were reviewed.The demographic characteristics,progress of KS,extent of lesions,treatment and prognosis of these patients were retrospectively studied.The efficacy of different therapies and the prognosis were compared using t or x2 test.Results The average age of 70 AIDS-KS patients was (40 ± 10) years,and the ratio of male and female was 2.5 ∶ 1.Sixty-six out of 70 (94.29％) patients were Uyghur ethnic.Among 70 patients,23 cases (32.9％) were rapidly progressed.Lesions often progress to plaques and nodules affecting head,neck,trunk,limbs and internal organs.Among 64 evaluable cases,the fatality rate in treatment group was 7.7 (4/52),which was significantly lower than that in patients who did not receive treatment (4/12,x2 =5.861,P ＜ 0.05).After 6-month of HAART or HAART sequenced by chemotherapy,CD4+ T lymphocyte counts were significantly increased (t =35.258 and 42.528,P ＜0.01),while it was decreased or remained in low level in patients received other treatments or untreated.HAART sequenced by chemotherapy,chemotherapy sequenced by HAART,and chemotherapy alone were effective for all AIDS-KS patients,while the effective rate in patients received HAART alone was 86.4％ (19/22),and immune reconstitution inflammatory syndrome (IRIS) was appeared in 45.5％ (10/22)patients.Conclusions AIDS-KS more frequently occurs in young Uighur males.The lesion may affect various organs,and some progress rapidly.HAART plus chemotherapy may reduce the incidence of IRIS and improve the prognosis.

BACKGROUND:The umbilical cord mesenchymal stem cells possess multipotent differentiation capacity, but less research focus on its differentiation into fibroblasts.
OBJECTIVE:To investigate the capacity of human umbilical cord mesenchymal stem cells differentiating into fibroblasts.
METHODS:Using adherent method, human umbilical cord mesenchymal stem cells were isolated, and flow cytometric analysis of the surface antigen was performed. Passage 3 cells were selected for osteogenic and
adipogenic differentiation, and cells differentiated into fibroblasts under the induction of basic fibroblast growth factor. RESULTS AND CONCLUSION:Adherent stem cells were stably isolated from the umbilical cord. Human umbilical cord mesenchymal stem cells lowly expressed CD31, CD45, CD40, HLA-DR, but strongly expressed CD29, CD90, CD44, CD105. Oil red O staining showed red droplets were ful of the cytoplasm after adipogenic induction;alizarin red staining showed red calcium nodules after osteogenic induction. After induced by basic fibroblast growth factor, the type I col agen expression was significantly higher than that in the control group. These findings indicate that the adherent human umbilical cord mesenchymal stem cells are reliably isolated with high purity;basic fibroblast growth factor can induce differentiation of umbilical cord mesenchymal stem cells into fibroblasts.