Locally advanced non-small cell lung cancer (LA-NSCLC) usually is inoperable because of tumor progress.At present,concurrent chemoradiotherapy has become standard regimen of these patients,which has gotten remarkable effectiveness.This article is a summarization on concurrent chemoradiotherapy for LA-NSCLC.

The investigations on the interactive relationships of DNA replication, damage repair and recombination have been locating in the frontline and becoming one of the hotspots in today's life science research. More and more studies show that the processes of DNA replication, damage repair and recombination are both independent and interdependent in the molecular level. These pathways coordinate and conform each other through interactions of many critical proteins in the pathways, by which DNA molecules, known as genetical materials, can be well maintained in cell and faithfully transferred through cellular generations. By using E. coli as a model system, the recent progresses and the possible rules underlying E. coli DNA replication, repair and recombination have been analyzed. As it is believed that the researches on E. coli DNA replication, repair and recombination may be capable of providing clues to the eukaryotic research based on the universal conservations of the critical proteins in the pathways.

Objective To compare the biocompatible and osteconduetian behavior of the high-density porous polyethylene and hydroxyapatite/ultra-high molecular weight polyethylene. Methods Osteoectomy of the upper orbital rim was performed on 60 adult Japan rabbits. The animals were randomly divided into 3 groups with 20 of each. The hydroxyapatite/ultra-high molecular weight polyethylene, high-density porous polyethylene was implanted to the upper orbital defect of the animals in respective group. Animals were sacrificed at the 1st, 2nd, 4th and 8th weeks. Alkaline phosphatase (ALP) and Osteocalcin (OC)in the HDPE and hydroxyapatite/ultra-high molecular weight polyethylene was also detected. Result During the experimental period of 8 weeks, there was no implants extrusion or displacement. Fibrous tissue and new vessels grew into HDPE were obviously elder than those grew into hydroxyapatite/ultra high molecular weight polyethy-lene. Alkaline phosphatase and osteocalcin contents has great difference, HDPE group is the best, followed by HA combination artificial group (P<0.05). Conclusions High-density porous polyethylene and hydroxyapatite/ultra-high molecular weight polyethylene are both good orbital reconstruction materials, they all have good biocompatiblity and osteoconductian, but high-density porous polyethylene is better than hydroxyapatite/ultra-high molecular weight polyethylene in osteoconductian.

Objective To analyze antimicrobial use in the different grades of hospitals within 3 years after the car-rying out of rectification activities of antimicrobial use,and provide basis for making continuous improvement pro-gram of antimicrobial management.Methods Retrospective analysis was performed,outpatient and emergency pre-scriptions and medical records of discharged patients (100 were type Ⅰ incision surgery)were selected from 5 tertia-ry hospitals and 5 secondary hospitals within 3 months,antimicrobial use and management were investigated. Results 200 outpatient prescriptions,100 emergency prescriptions,and 200 medical records of discharged patients were selected from each hospital.In tertiary and secondary hospitals,antimicrobial usage rate in outpatient prescrip-tions was 17.40% and 18.00% respectively,emergency prescriptions was 31 .60% and 40.10% respectively;a-mong inpatients,antimicrobial usage density(AUD)was (28.13±8.04)and (49.67±11 .63)respectively,and anti-microbial usage rate was 41 .30% and 56.20% respectively;pathogenic detection rate of antimicrobial therapeutic cases was 45.70% and 22.70% respectively,therapeutic cases with limit level of antimicrobials was 61 .50% and 27.40% respectively;antimicrobial usage rate in type I incision surgery was 20.00% and 40.40% respectively;The rational rate of antimicrobial types was 94.60% and 59.20% respectively,rational rate of opportunity of antimicro-bial use was 95.20% and 48.20% respectively;combination rate was 8.80% and 36.60% respectively,and rational rate of treatment course was 84.00% and 52.80% respectively.Conclusion In tertiary hospitals,antimicrobial use after 3 years rectification of antimicrobial use conform to the antimicrobial management requirement of Ministry of Health,the overall quality index is better than secondary hospitals,management of antimicrobial in secondary hos-pitals use should be improved.

The aim of this study is to investigate the effect of fluoxetine (FLX) on the expressions of BDNF and Bcl-2 in the hippocampus, the amygdala and the prefrontal cortex of conditioned fear (CF) model mice. Forty eight mice were randomly divided into three groups, normal control group, CF stress group and FLX-pretreated CF group. The FLX-pretreated CF group was given FLX (10 mg x kg(-1) x d(-1)) for 7 days before CF stress. After CF stress model was established, all mice were given behavioral experiments to test whether FLX impaired or improved the auditory and contextual fear conditioning. Then mice were sacrificed. The expressions of BDNF and Bcl-2 were detected by Western blotting. The results showed that the freezing time of FLX-pretreated CF group was significantly lower than that of CF group; FLX pretreatment up-regulated the expression of Bcl-2 in the hippocampus at 1 d after CF stress (P < 0.001), but no significant differences was observed at 7 d; BDNF significantly increased in the hippocampus at 7 d (P < 0.001), but no differences at 1 d; the expressions of BDNF and Bcl-2 in the amygdala and the prefrontal cortex were of no obvious differences between CF group and FLX-pretreated CF group at 1 d or 7 d after CF stress. Parallel to these changes, pretreatment with FLX could affect histopathologic changes induced by CF stress. Furthermore, the results indicated that FLX pretreatment could protect against CF stress-induced neurological damage via the activation of BDNF and Bcl-2 in hippocampus.

Objective To observe the effect of extracts from Flos Lonicerae inhibiting influenza A virus FM1 strain. Method Use the method of hemagglutination test, determine the hemagglutination inhibition titer that extracts from Flos Lonicerae inhibiting influenza A virus in vitro and in embryonated egg. Result Compared with the control group, the hemagglutination inhibition titer of extracts from Flos Lonicerae group were degraded. The depressant effect kept on from 1 hour to 24 hour, and the depressant effect were degraded with the stepping down of the concentration. At the concentration of 100, 50, 25 mg/mL, extracts from Flos Lonicerae had the preventative effect and therapeutical effect on embryonated egg infected by influenza A virus (P

Objective To investigate the prevalence and influencing factors in senile cataract in Huzhou city.Methods 1 680 cases of healthy physical examination elderly population were selected as the research subjects in Huzhou city.To investigate the prevalence of cataract in the elderly population.The correlation of the prevalence of senile cataract,visual impairment and sex,age was studied.The correlation of the prevalence of senile cataract and diabetes,hypertension,smoking was analyzed.At the same time,Logistic regression analysis was used to analyze the influence factors of senile cataract.Results In 1 680 cases of elderly people,299 cases of cataract,the prevalence rate of cataract was 17.79%.With age increased,the prevalence of senile cataract was gradually increased.The preva-lence of cataract in female was 21.80%,which was higher than 15.82% in male,and the difference was statistically significant (χ2 =9.083,P <0.01).The visual acuity of senile cataract patients would increase with age.Moreover, the visual acuity of female patients was higher than that of male patients.The prevalence of cataract in diabetic group was 64.58%,which was higher than 17.89% in non -diabetes group,the difference was statistically significant (χ2 =65.457,P <0.01 ).The prevalence of cataract in hypertension group was 61.48%,which was higher than 14.31% in non -hypertension group,and the difference was statistically significant (χ2 =163.538,P <0.01).The prevalence of cataract in smoking group was 80.39%,which was higher than 16.94% in non - smoking group,the difference was statistically significant (χ2 =130.046,P <0.01).Logistic regression analysis showed that gender,age, diabetes,hypertension,smoking,and so on were the factors affecting the incidence of cataract in the elderly popula-tion.Conclusion The prevalence of cataract in the elderly population in Huzhou city need to pay attention to the local health department.

Objective To understand antimicrobial resistance and homology of carbapenem-resistant Klebsiella pneumoniae (CRKP)from an intensive care unit (ICU).Methods 11 CRKP isolates from patients and environment of an ICU in a hospital were performed antimicrobial susceptibility testing,the homology of CRKP was analyzed by randomly amplified polymorphic DNA (RAPD)method.Results Of 11 CRKP isolates,8 were from patients,and 3 from environment,the main specimen was sputum(n =6,54.55%).Antimicrobial susceptibility testing results re-vealed that 10 (90.91%)CRKP isolates were resistant to ciprofloxacin;11 isolates were susceptible to compound sulfame-thoxazole,intermediate to imipenem,and resistant to other antimicrobial agents(100%).All 11 CRKP isolates had 3 bands,and were divided into two types:(typeⅠ,n=10;type Ⅱ,n=1).Conclusion Antimicrobial resistance of Kleb-siella pneumoniae in ICU is serious,CRKP isolated from ICU patients and environment are of the same clone. Cleaning,disinfection,and monitoring of ICU environment should be strengthened,which is helpful for reducing, timely warning of multidrug-resistant organisms,and reducing healthcare-associated infection.

Aim To investigate the effect of ASX (trans-astaxanthin)on the expressions of NF-κB p65 , iNOS and TNF-αin the hippocampus and the prefron-tal cortex of chronic alcohol mice.Methods 40 mice were randomly divided into control group,7 d,14 d, 21 d,28 d alcohol-treated group,the mice were given alcohol preference testing on day of 6,13,20,27. Mice were subjected to alcohol withdrawal for one day after testing.In order to determine the exact time point of cognitive memory impairment in mice after alcohol consumption,they were given morris water maze test after alcohol preference testing. The other 40 mice were randomly divided into control group, alcohol group and ASX group (20,40,80 mg·kg-1 ).After chronic ASX administration, mice were given one probe trial of 60 s in which the platform was removed from the pool to evaluate escape latency,the number of times the animal crossed the previous location of the platform,time spent in the target quadrant,and swim-ming speed.The expressions of NF-κB p65 ,iNOS and TNF-αwere detected by western blotting after behav-ioral testing.Results The mice showed an obvious al-cohol-related phenomenon on 2 1 and 28 days after al-cohol treatment,and escape latency significantly in-creased,entries in target quadrant and duration in tar-get quadrant significantly decreased with increasing drinking days and withdrawal times.The results also suggested that 2 1 days chronic ASX treatment reversed this learning deficit.Moreover,the expression of NF-κB p65 ,iNOS and TNF-αin the hippocampus were significantly increased after 2 1 d alcohol treatment (P<0.001),and pretreatment with ASX (40,80 mg· kg-1 ) could obviously inhibit these changes (P <0.001);Parallel to these changes in the hippocam-pus,the level of NF-κB p65 ,iNOS and TNF-αwere also increased in the prefrontal cortex (P<0.001 ), however,only ASX (80 mg · kg-1 ) administration could inhibit the increase (P<0.05 ).Conclusion These results indicate that ASX pretreatment can pro-tect against alcohol-induced memory impairment via the inhibition of NF- κB p65 ,iNOS and TNF-αexpres-sions in hippocampus and prefrontal cortex.

Objective To investigate the effective administration model for gestational diabetes mellitus (GDM) in the community. Methods In a prospective study, the 75 g oral glucose tolerance test (OGTT) was performed in 4 713 resident pregnant women over 20 years old who received antenatal care in a general hospital or a special hospital from Sep. 2011 to Aug. 2012. Five hundred and thirty-three pregnant women were diagnosed as GDM, 198 patients who labored in a general hospital were enrolled in thegroup A , and the rest who labored in a special hospital were enrolled in the group B. 198 cases with non-GDM were enrolled in the group C. Results The incidence of GDM during this study period was 11.3%. The maternal age , gestationl weeks and OGTT results of patients in the three groups were significantly different (P < 0.05). With the increase of maternal age of pregnant women, the blood glucose increased while the gestationl weeks descreased. No significant differencees were shown in prevalence of polyhydramnios, hypertensive disorder complicating pregnancy, premature labor, macrosomia , fetal growth restriction and neonatal asphyxia among the three groups (P > 0.05). However, significant differences were found in the incidence of postpartum hemorrhage and cesarean section. The incidence of postpartum hemorrhage increased significantly in the patients of group B (χ2= 7.156, v = 2, P = 0.028). The incidence of cesarean section increased significantly in the patients of group A (χ2= 63.592, v = 2, P = 0.000). Conclusion Establishing an effective administration model for gestational diabetes mellitus in the community could control the incidence of GDM associated complications.