1.Antiepileptic drug-induced overexpression of P-glycoprotein multidrug resistance gene in astrocytes in vitro
Yang LV ; Yong YAN ; Xuefeng WANG ; Al ET
Chinese Journal of Neurology 2001;0(01):-
Objective To study the overexpression of antiepileptic drug (AED) induced P glycoprotein multidrug resistance gene in astrocytes in vitro Methods Postnatal Wistar rats (within 24 h) astrocyte cell culture was performed. Different concentrations of phenytoin (PHT),phenobarbitone (PB),carbamazepine (CBZ),valproate (VPA) were added to the culture media for 10,20 and 30 days The overexpression of P glycoprotein (Pgp) multidrug resistance gene was investigated with the immunocytochemistry method Results The Pgp staining of normal astrocytes was less than 5% at each time point The Pgp positive staining was found in 20 mg/L PB,40 mg/L PB,20 mg/L PHT at the 30 day There were overexpressions at the 20 and 30 day, excluding CBZ at 20 day in the higher concertration of the four AED Conclusion AED may induce the overexpression of Pgp,which is correlated with the dose and time
2.Preparation of immunoglobulin Y against avain influenza virus(H_5N_1)and studies on the inhibitory effect to influenza virus A (FM_1) in vitro
Xuefeng LV ; Zhaohui NI ; Shihong YI ; Fan LI
Chinese Journal of Immunology 2001;0(10):-
Objective:To prepare the immunoglobulin Y(IgY) against Avain Influenza Virus (H5N1) and to investgate its inhibitory activity to Influenza virus A (FM1).Methods:Iimmunized by Avain Influenza vaccine,IgY against Avain Influenza Virus was purified from the yolk and then the antibody was enriched by the means:caprylate acid-two-step salt precipitation-gel chromatograph.TD0 of IgY and the inhibory effectiveness were showed by nhibiting cytopathic effect (CPE) thst in MDCK infected with Influenza virus A(FM1).Results:IgY against Avain Influenza Virus was obtained.The TD0 value of IgY against Avain Influenza to MDCK cell line was 1.764 mg/ml,while at the dosage as low as 0.082 8 mg/ml the lnfluenza virus A(FM1) was still inhibited in vitro.Connchusion:Caprylate acid-two-step salt precipitation-gel chromatograph can prepare IgY successfully and the inhibitory effectiveness of IgY is good for inhibition of Influenza virus A(FM1).
3.Laparoscopic left lateral sectionectomy using the Glissonian pedicel approach: a study of 8 patients
Liuxin CAI ; Zheping FANG ; Minling CHENG ; Xuefeng DU ; Zhenyu LI ; Shangdong LV
Chinese Journal of Hepatobiliary Surgery 2012;18(6):416-419
Objective To study the safety and feasibility of laparoscopic left lateral sectionectomy using the Glissonian pedicel approach.Methods The root of the round ligament of the liver was exposed and the Glissonian pedicel of the left lateral section was dissected,starting from left and using the lapa roscopic Peng's multifunctional operative dissector (LPMOD).After the Glissonian pedicel of segment Ⅱ and Ⅲ was dissected,clipped and cut,the ischemic boundary showed up.The liver was transected at the boundary of the ischemic liver,and the left hepatic vein and its branches were cut. Resutts The surgery was successtully performed in 8 patients.There was no conversion to open operation.The operative time was 110- 190 (151.0±35.4) min.The time of Glissonian pedicel dissection and liver resection was 70- 135 (101.0±24.1) min.Operative blood loss was 100-300(210.0± 89.4) ml.The ALT increased by 35- 102 (75.4± 26.5) U/L after operation and decreased to a normal level in 2-6 (3.0± 1.7) d.The postoperative hospital stay was 6-10 (8.2± 1.6) d.There was no major complication.Conclusion Laparoscopic left lateral sectionectomy using the Glissonian pedicel approach is safe and feasible.
4.Bone morphogenetic protein 2 poly lactic acid sustained release microspheres for compatibility between rabbit bone marrow mesenchymal stem cell research
Likun MA ; Jiang DENG ; Wenliang HUANG ; Peng YE ; Renyuan TIAN ; Xuefeng LV
Chongqing Medicine 2015;(8):1022-1024,1028
Objective To investigate the influence of recombinant human bone morphogenetic protein‐2(rhBMP‐2)of poly lactic acid(PLA) release microspheres for compatibility of rabbit bone marrow mesenchymal stem cells(BMSCs) .Methods The rh‐BMP‐2‐PLA release microspheres were prepared by w/o/w multiple emulsion volatilizing method and then cocultured BMSCs .The effects of rhBM P‐2‐PLA release microspheres on the cytotoxicity and relative proliferation rate by MTTassay .Evaluation of mate‐rials biocompatibility by inverted microscope and scanning electron microscope(SEM) .Results The rhBMP‐2‐PLA release micro‐spheres in various concentration of leaching solution and BMSCs training of uninfected cells .Experimental group and control group in 4 different time cell proliferation OD values by analysis of repeated measurement variance between time OD values were statisti‐cally significant(P=0 .000) ,the experimental group and control group OD values are statistically significant(P=0 .025) ,the exper‐imental group higher than the control group ,experimental group OD value time there was a significant interaction effect and the group number ,the change trend are obviously different(P=0 .006) .Inverted microscope to observe materials normal cell prolifera‐tion ,SEM found that vaccination cells surrounding rhBMP‐2‐PLA release microspheres of 7 days later ,the cells grew well and split proliferation activity .Conclusion rhBMP‐2‐PLA release microspheres of BMSCs has non‐toxic and has compatibility .
5.Metabolism of triglyceride in plasma of rabbits with necrosis of femoral head induced by hormone and therapeutic effect of traditional chinese medicine
Kaiyun CHEN ; Xiuhua LV ; Zhen LIU ; Hongxin LIU ; Wei LIU ; Ping AI ; Xuefeng YU
The Journal of Practical Medicine 2017;33(2):206-209
Objective Toinvestigate the metabolic characteristics of triglyceride (TG) in the plasma of rabbitswith steroid?induced osteonecrosis of the femoral head and the therapeutic effect of Gushiling Capsule. Methods Rabbit model of femoral head necrosis induced by hormone was established. The level of plasma TG was detected by HPLC/MS and the relationship between the changes of plasma TG level and local histomorphology of femoral head was analyzed. Results Compared with the normal control group, significant changes of plasma TG levels were observed in the model group, the traditional Chinese medicine group and the western medicine group at each time point (P<0.01). However, no significant difference was found between the traditional Chinese medicine group and the western medicine group. Compared with the model group, only a small amount of high signal joint imaging and central signal of the femoral head were found by the MRI examination in the traditional Chinese medicine group and the western medicine group. Pathology assay results showed the degree of femoral head osteonecrosis in the Chinese medicine group was milder than that in the Western medicine group. Conclusion Gushiling Capsule and western medicine Simvastatin have the similar effect of reducing plasma TG, which may be one of the mechanisms underlying Gushiling Capsule preventing steroid?induced femoral head necrosis.
6.Effects of environmental hypothermia on hemodynamics and oxygen metabolism in unanesthetized swine model of hemorrhagic shock
Cheng ZHANG ; Guangrong GAO ; Huiyong JIANG ; Chenguang LV ; Baolei ZHANG ; Mingshuang XIE ; Zhili ZHANG ; Li YU ; Xuefeng ZHANG
Chinese Journal of Emergency Medicine 2011;20(10):1067-1071
Objective To investigate the effects of environmental hypothermia on hemodynamics and oxygen metabolism in unanesthetized swine model of hemorrhagic shock.Methods A total of 16 Bama pigs provided by animal experiment centre of the General Hospital of Shenyang Military Command were randomized into two groups ( n =8,each):ambient temperature (A) and hypothermia ( H ).Venous blood (30 mL/kg) was continously withdrawn over 15 minutes to establish hemorrhagic shock model.Core temperature,heart rate,mean arterial pressure,central venous pressure,cardiac output,saturation of mixed venous blood and blood gas analysis were recorded at the baseline and different hemorrhagic shock time.DO2I and VO2I,and the O2 extraction ratio (O2ER) were calculated.Results Core body temperature in group A decreased slightly after hemorrhagic shock model had established and environmental hypothermia resulted in more reduction in core body temperature.The mortality rate was significantly higher in group H (50%) than in group A (0%) (P <0.05).DO2I and VO2I decreased significantly after hemorrhage.No difference was found in hemodynamics,DO2I and VO2I between group A and group H,but the difference of pH,lactic acid and O2ER were significant between the two groups (P < 0.05 ).conclusions Environmental hypothermia aggravated the disorder of oxygen metabolism after hemorrhagic shock,which was associated with poor prognosis.
7.Remote surveillance system of artificial ventricular assist device.
Jingjing LI ; Ming YANG ; Xuefeng LV
Chinese Journal of Medical Instrumentation 2011;35(1):1-5
Performances of reliability and portability are important for artificial ventricular assist devices. This paper presents a remote surveillance system that can observe the condition of the patients and the driving condition of artificial heart online. The system is mainly based on the embedded Compact RIO platform and Ethernet technology. Combined with the driver module of the assist device, this remote system has been tested.
Equipment Design
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Heart, Artificial
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Heart-Assist Devices
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Remote Sensing Technology
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Robotics
8.Finite element analysis of the three and four cannulated screws for Pauwels III femoral neck fractures
Hao ZHANG ; Xuefeng SHI ; Chunbao YANG ; Jihong LV ; Yi XIANG ; Zhenjun SUN ; Jingtao GUAN ; Yong TANG ; Limin XU
Chinese Journal of Tissue Engineering Research 2016;20(26):3897-3902
BACKGROUND:Femoral neck fracture is mainly fixed by three inverted triangle cannulated screws. Scholars have proposed to add a cannulated screw to enhance the fixation strength of femoral neck fracture of Pauwels III type based on three cannulated screw fixation, but the stability is not verified. OBJECTIVE:To analyze the biomechanical stability and stress of the three and four cannulated screws for the treatment of the Pauwels III femoral neck fractures. METHODS:The CT imaging results of the fourth generation of artificial bone sawbones were imported into the Mimics software wherein a three-dimensional finite element model of the proximal femur was prepared and introduced in the 3-matic software. Models of middlesegment of femoral neck with Pauwels III fractures were established. Cannulated screw models were established with UG 8.0 software and introduced in the fractures models. Finally, finite element models of Pauwels III femoral neck fractures fixed with three and four screws were established. In the same condition, an axial load of 411 N was applied on the femoral head with Abaqus software. The displacement of two markers of the broken ends and internal fixation system Von Mises stress distribution were compared between the two models. RESULTS AND CONCLUSION:(1) The displacement was 0.42 mm in three screws model, and 0.17 mm in the four screws model. (2) Von Mises stress peak was 547 MPa and 27.8 MPa in both models. The peak value was lower in models of fourscrews than that of three screws. Stress concentration position was at the fracture site in both models. The stress range of models of four screws was more extensive and scattered. (3) Finite element analysis results demonstrated that four-screw implantation for Pauwels III femoral neck fractures had strong anti-shearing force and biomechanical stability. Clinical advantages need further clinical comparative study.
9.Clinical Effect and Safety Study of Modified Qing-Fei-Jie-Du Decoction on Lobar Pneumonia in 60 Cases of Children
Guihua SONG ; Zhiwei GUAN ; Yan ZHANG ; Weigang LV ; Suping YU ; Yanrong GUO ; Wei ZHANG ; Xuefeng WANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(6):1072-1075
This study was aimed to investigate the clinical effect and safety of Qing-Fei Jie-Du (QFJD) decoction on treating lobar pneumonia in children.Ninety cases of children diagnosed with lobar pneumonia in hospital were selected by double-centre,random and control method.Cases were divided into the test group (60 cases) and the control group (30 cases.The ratio between the test group and the control group was 2∶1.It can fully demonstrate the effect of the test group without any influence on the statistical analysis).Children of the control group were given conventional medicine with placebo,while children of the test group were given conventional medicine with modified QFJD decoction.The treatment course was 2 weeks.The clinical symptoms including fever,cough,lung rale,lung image (X-ray or CT scan),laboratorial index and safety were compared between two groups.The results showed that the recovery rate of the test group was 81.7%;and the recovery rate of the control group was 66.7%.The difference between them was statistically significant (P<0.05).The comparison of safety between two groups indicated that the treatment of the test group was safer than control group with statistical difference (P<0.05).It was concluded that modified QFJD decoction had a significant clinical effect and was very safe in the treatment of children with lobar pneumonia.
10.An XcmⅠ-generated T vector and its applications in synthetic biology.
Yangkai DUAN ; Feiyan LIANG ; Xiaoming TAN ; Xuefeng LV
Chinese Journal of Biotechnology 2016;32(7):956-965
For more economical and efficient DNA clonging, pFL-XS-T, a Biobrick-T vector was constructed based on pMD18-T vector, carrying clonging regions of XbaⅠ-XcmⅠ-XcmⅠ-SpeⅠ. The results revealed that PCR products could be conveniently inserted into pFL-XS-T vevtor digested by XcmⅠby means of TA cloning. The positive frequency of recombination can meet the experimental requirements and all the plasmids obtained meet Biobrick standard. Moreover, the pFL-XS-T is compatible with other Biobrick parts, and serves as a vector for functional DNA fragments screening.
Cloning, Molecular
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DNA
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Genetic Vectors
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Plasmids
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Polymerase Chain Reaction
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Synthetic Biology