OBJECTIVE:To explore the effects of pioglitazone on secreation and mRNA expressions of TNF-? in adipocytes of high-cholesterol model rats.METHODS:30 rats fed with high-cholesterol diet for 8 weeks were randomly assigned to receive high cholesterol diet alone(high-cholesterol group,n=15) or high-cholesterol diet plus pioglitazone(3 mg?kg-1?d-1) (pioglitazone group,n=15) for 4 weeks.Another 15 rats were assigned to control group and fed with common diet for 12 weeks.Then the rats were sacrificed,with indexes such as blood lipid,serum concentration and mRNA expression of TNF-? in adipocytes of high-cholesterol rats determined.Besides,normal adipocytes were taken and treated with lipopolysaccharide and different concentrations of pioglitazone (0.1,1.0,10.0 ?mol?L-1) followed by a determination of TNF-? level and mRNA expression level.RESULTS:Compared with high-cholesterol group,serum concentration and mRNA expression of TNF-? in pioglitazone group were significantly lowered,but pioglitazone treatment showed little impact on blood sugar level and blood lipid level.Pioglitazone(0.1,1.0,10.0 ?mol?L-1) dose-dependently inhibited lipopolysaccharide-induced TNF-? secretion and mRNA expression in cultured adipocytes.CONCLUSION:Pioglitazone can reduce TNF-? level of fatty tissue and serum in high-cholesterol model rats.

OBJECTIVE:To improve the quality of GSP certification and accelerate the proceedings of certification in China.METHODS:This paper introduces the current situation in the certification,also points out the existing prob?lems.RESULTS&CONCLUSION:Goverment concerned should strength the GSP training and the leaders in the drug busi?ness enterprises must attach importance to GSP certification and value the training inside the enterprises to raise the quality of staff,also they must keep the same level after passing GSP certification.

OBJECTIVE：Probe into the feasibility of preparation of diclofenac sodium with β-cyclodextrins in 1∶1 and 2∶1 inclusion compounds ,and find out the affection in solubility of diclofenac sodium. METHODS:The 1∶1 and 2∶1 melusion compounds were prepared by liquid-phase an alysis the content and determination them solubility.RESULT:The 1 ∶1 and 2∶1 inclusion compounds were prepared by different ratio of reactants and etermined them mp were 299～300.5℃ and 302～303℃ respectively. CONCLUSION：Of us is that solubilits of the 1∶1 and 2∶1 inclusion compounds increase 0.65 and 1 times respetively comparing with declofenac sodium.

Objective To investigate the effect of dietary and exercise on the incidence of GDM in women with pre-pregnancy obesity. Methods One hundred and sixty early pregnant women with BMI≥25 kg/m2 in our hospital from April 2013 to January 2015 enrolled in the study were divided into two groups: The eighty women hospitalized during June 2013 to January 2014 were assigned as the control group, while the other 80 women from February 2014 to January 2015 as the study group. In the former group, the routine pregnancy inspection and pregnancy health education seminars were conduced during pregnancy of 12 weeks , and in the study group weekly nutritional assessment , diet planning by a dietitian and exercise guidance by a professional nurse were performed apart from the same care as in the control group. The two groups were compared in terms of weight growth and GDM incidence. Result The weight increased and the GDM incidence in the study was both significantly lower than those in the control group ( P < 0 . 05 ) . Conclusion The dietary and exercise during pregnancy can effectively control the weight and decrease the incidence of GDM , which can ensure the health of pregnant women.

Objective To investigate the effect of decitabine on serum platelet derived growth factor levels in patients with leukemia. Methods 120 cases of acute myeloid leukemia patients admitted to our hospital from January 2012 to December 2014 were selected as the study subjects.The control group was given CAG regimen: 1-14 d Seventh days of intravenous infusion of 20 mg/d aclarubicin, first to fourteen days subcutaneous injection of 10 mg/m2 , 1 times per 12 h, 1-13 d cytosine arabinosine days under the subcutaneous injection of granulocyte colony stimulating factor 300μg/d.The observation group was given to the west of the lake 25 mg/d, intravenous infusion, used for 5 days.The levels of platelet derived growth factor PDGF in the two groups were compared and the therapeutic effect.Results Before treatment, differences of PDGF in control group and observation group was no statistical significance.After treatment, PDGF in observation group was lower than control group ( P <0.05).The proportion of primary cells of bone marrow of two groups after treatment was lower than before treatment, and the observation group was more lower.Total effective rate of observation group was 55.0%,which was higher than that in control group(35.0%)(P<0.05).Conclusion Decitabine could reduce the level of of PDGF in patients with leukemia, and improve the total effective rate.

Objective To explore the epidemiologic al features of emergency calls around the spring festival holiday in past 5 years,so as to guide emergency medical services during holldays.Methods An investigation was made into emergency calls around the spring festival holiday in 2001～2005(1～7 of the first month of the lunar year) as well as one week before and after the holiday.Results The person-times to emergency during the spring festival holiday was correspondly stabilize in the recent 5 years,compared to the datas of the previous and subsequent 1 week,the person-times of emergency and the patients needed to rescue at first significantly increased,and the patients to pediatric emergency increased most significantly.The most part of the person-times to emergency were internal medicine.Conclusion It is essential to do a good job of health education and disease prevention,especially among high risk groups of people,and to reinforce the establishment of the emergency medical services system so as to effectively guarantee the the development of holiday economy.

0.05).Conclusion:The CT perfusion parameters can be regarded as a surrogate marker of tumor angiogenic activity,which is a useful method in estimating the degree of angiogenesis.

Objective To study the effect of Shengmai injection on the platelet parameters and CD4+CD25+cells in peripheral blood of patients with chronic lymphocytic leukemia,and to explore the optimal regimen for patients with chronic lymphocytic leukemia.Methods One hundred and twenty patients with chronic lymphocytic leukemia were enrolled in this study from January 2012 to December 2014.(D1),vincristine 4mg(d1),prednisone 60 mg/d(d1-5)were given to the CHOP regimen in the control group: cyclophosphamide 600 mg/m2(d1),doxorubicin 25 mg/m2(d12)28d a course of treatment,a total of 4 courses,the observation group of patients in the control group of patients treated on the basis of Shengmai injection,compared the two groups of patients with chemotherapy,platelet parameters and serum CD4+CD25+T cells and Th17 cells.Results After treatment,the PLT,MPV and PDW of the observation group were(215.4± 31.7),(9.5±2.5)and(16.9±2.4),respectively,which were significantly higher than those of the control group.The CD4+CD25+T cells in the observation group were(1.5±0.8)The total effective rate of the control group was 35.0％,the total effective rate was 35.0％,and the total effective rate of the control group was 35.0％.There were significant difference between the control group and the control group(P<0.05),The difference was statistically significant.Conclusion Shengmai injection combined with CHOP regimen in chronic lymphocytic leukemia patients can improve immune function,promote platelet growth,improve platelet clinical parameters,and help improve the efficiency of chemotherapy,compared with CHOP alone Program treatment is better,worthy of clinical application.

Cardiovascular Diseases ; Humans ; Immunoglobulin G