Objective:To study characteristics of the middle school students' associating with their parents, teachers and classmates Method:1850 middle school students in Xianning city were surveyed with a self-designed questionnaire Result:The rate of behavior problems of students associating with their parents, teachers and classmates were 12 3%, 1 9% and 3 8% respectively Boys had more behavior problems in all associations than girls Students from single child family had no more behavior problems than those from multiple children family had, while students from city or town had more than students from villages of rural areas had The adopted children had more problems with their adopting parents or teacher than the others did with their parents or teachers Conclusion:The results suggest that boys be earlier than girls in socialization There is urban-country difference in association behavior of middle school students

0.05).[Conclusion]The contents of CRP,IL-1,IL -6,TNF-? are strengthenly expressed within 1 hour and have different expressions in different areas.

Objective To evaluate the effects of transplant adductor brevis and gracilis to correct scissors gait in spastic cerebral palsy. Methods From July 2000 to December 2004, 73 patients with scissors gait of spastic cerebral palsy were treated with transplantation of adductor brevis and gracilis, 47 were males and 26 were females, with the average of 12.06 years old. According to Ashworth's standard, the muscle tension was evaluated before surgery. 12 cases were in degree Ⅰ, 38 cases were in degreeⅡ, 18 cases in degree Ⅲ and 5 cases in degree Ⅳ. The end of adductor brevis was cut off and sutured with tensor fasciae latae through the tunnel under the skin, the end of gracilis was cut off and transplanted to the lateral condylus of femur through the back of the knee. Some certain surgery completed at the same time, the semitendious and biceops muscle was transplanted to the front of the knee(37 cases) and extended(13 cases), the soft tissue was released in hip joint(55 cases), the gastrocnemius cut off (32 cases), the Achilles's tendon extended(30 cases) and tibialis anterior lateral transplantation (31 cases), only peroneus longus lateral transplantation (8 cases). After operation the lower limbs was fixed with plaster cast for 6 weeks, then the plaster cast was removedassociating with functional exercise. The orthopaedic results were evaluated on the function of hip, knee, foot-malleoious and the gait, then classified four grades. Results All patients were followed up an average of 24.4 months. The scissors gait was corrected completely, 61 cases were excellent, 10 cases were good, 2 cases were fair. The majority symptom of whem was the knee flexure, but can be relieved by medicine. There were significant differences compared pre-operation with post-operation in four groups, and also between degree Ⅳ group and degree Ⅰand Ⅱgroup(P

Patients with spinal cord injury would suffer many complications including hip abnormities. Few reports on spinal cord injury with hip dislocation had been published because of a lower incidence and difficulty to be found in clinical practice. This paper reviewed epidemiology, etiology, diagnosis, prevention and treatment of hip dislocation after spinal cord injury.

BACKGROUND:With the development of industrial society and traffic, the incidence of spinal cord injury has gradualy increased. In addition to radiological laboratory examinations, the neurophysiological test also becomes an effective way of auxiliary examination. Due to the high accuracy and easy operating, neurophysiological test is widely used and the evoked potentials play a role in this project. OBJECTIVE:To summarize the application of somatosensory evoked potentials and motor evoked potentials in rabbits of spinal cord injury. METHODS:A computer-based online research of CNKI and PubMed databases was performed with the key words of “spinal cord injury; evoked potentials; animal models” in Chinese and English. Finaly 33 articles were included in the analysis according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION:The operational approach, waveform analysis, application value, influencing factor of somatosensory evoked potentials and motor evoked potentials were determined in details, the correlation between evoked potentials and prognosis assessment in animals was also detected. Somatosensory evoked potentials is a good indicator for the evaluation of dorsal funiculus of spinal cord, it is easy to operate and reflects the changes of sensory functions. Motor evoked potentials can provide sensitive diagnosis of spinal cord diseases, and they wil be used as a means to assess the spinal cord injury during rehabilitation. The combination of the two can provide a more accurate result.

[Objective]To study therapeutic effect of different medicines on motorneuron of spinal cord after explosive injury of spinal cord.[Method]Thirty-six rabbits were randomly divided into model group(group A,n= 12),the dexamethasone experiment group(group B,n= 12) and the methylprednisolone experiment group(group C,n=12),all rabbits were made explosive wound by 0.9 g cyclotrimethylene trinitramine,group A injection NS after blast, group B injection dexamethasone and group C injection methylprednisolone,6 and 24 hours after specimen had been taken out,then morphological change and quantity of the spinal motorneurons be observed under light microscopes.[Result]The reversible change of the neurons of rabbits happened after six hours,after 24 hours,the died motoneuron increase obviously, some rabbits remedied with dexamethasone and methylprednisolone after explosive injury in group B and C,quantity of the died motorneuron marked significant difference than that in the control group(P0.05).[Conclusion]Glycocotical stdroid can protect spinal cord motoneurons after explosive injury of spinal cord.In the experiment,there is no preponderance in early treatment of explosive injury of spinal cord between methylprednisolone and dexamethasone.

The pollution of surface water and ground water caused by pharmaceuticals and personal care products(PPCPs) can directly affect the quality of drinking water.The sources of PPCPs and the routes to the drinking water were introduced briefly in the present paper.The removal efficiency of PPCPs in different unit processes applied in drinking water treatment was discussed in detail,including clarify,oxidization-disinfection,advanced treatment and so on.The performances of drinking water treatment unit processes for PPCPs removal were evaluated simply.

Objective To review the clinical characteristics of idiopathic portal hypertension (IPH).Methods The clinical and pathological data of 10 patients with idiopathic portal hypertension admitted from December 2008 to December 2014 were retrospectively analyzed.Results Among 10 patients 5 were males and 5 females with averaged age of (38.6 ± 16.1) years.There were splenomegaly and esophageal varices in all 10 cases,upper gastrointestinal bleeding in 3 cases,thrombocytopenia in 9 cases and anemia in 6 cases.Liver function was normal in 7 cases,mild abnormality in 3 cases.Ten cases underwent ultrasound examination and 7 cases had CT scan,cirrhosis was suggested with ultrasound/CT scan in 6 cases.Liver histology showed lobular architecture in existence,no false flocculus to form,variable degree of portal fibrosis appeared.Eight cases were misdiagnosed as liver cirrhosis,the duration of misdiagnosis varied from 1 month to 15 years.Conclusions The clinical manifestation of IPH is similar as cirrhosis caused portal hypertension.Liver histopathological examination can exclude liver cirrhosis,and portal fibrosis and liver terminal portal branch occlusion in histopathology is helpful to the diagnosis of IPH.

Objective To investigate the effect of over-expressed Cyr61 on the expression of extracellular matrix of human renal tubular epithelial cells(HKC), and explore the role of Cyr61 in the pathogenesis and development of autosomal dominant polycystic kidney disease(ADPKD). Methods Cyr61 gene cDNA was amplified by RT-PCR. A recombinant plasmid pcDNA3.1+ Cyr61 was constructed by cloning Cyr61 gene into pcDNA3.1. HKC cells were transfected with wild-type Cyr61 plasmid vector (pcDNA3.1+Cyr61). Then the fusion of Cyr61 gene and expression of its protein were detected by RT-PCR and Western blot. The mRNA expression of Cyr61, collagen Ⅰ, collagen Ⅳ, and laminin were determined in four groups (the untransfected cells, pcDNA3.1 transfected cells, pcDNA3.1+Cyr61 -transfected cells and cyst-lining epithelial cells) by fluorescence quantum PCR. Results The amount of Cyr61 protein in Cyr61-transfected cells and cyst-lining epithelial cells were much greater than the control. The mRNA expression of Cyr61, collagen Ⅰ, collagen Ⅳ and laminin in Cyr61-transfected cells were all amplified significantly, and the level of collagen Ⅳwas much higher than collagen I(P

AIM: To investigate the feasibility to inhibit the expression of MHCⅡ by special siRNA targeting class Ⅱ major histocompatibility complex (MHC Ⅱ) transactivator (CⅡTA), which might regulate MHC Ⅱexpression for suppressing immune rejection. METHODS: Five different siRNA were designed, synthesized and transfected into freshly isolated rat corneal keratocytes. At 24 hours posttransfection, the changes of MHC Ⅱexpression were detected by flowcytometry, and the mRNA abundance of CⅡTA and MHC Ⅱ was measured by FQ-PCR after inducing with recombinant rat interferon-gamma (IFN-?). RESULTS: Different siRNA showed different reduction in MHC Ⅱ and CⅡTA expression compared with the control (P