0.01). No serious complications occurred. Conclusion Both PLD+IDET and PLD are very effective in treating lumbar disc herniation. IDET is very helpful in relieving low back pain, although it can hardly improve patient's daily activities.

OBJECTIVE: The purpose of this study was to investigate whether three-dimensional (3D) magnetic resonance imaging could improve diagnostic accuracy for suspected posterior ligamentous complex (PLC) disruption. MATERIALS AND METHODS: We used 20 freshly harvested goat spine samples with 60 segments and intact surrounding soft tissue. The animals were aged 1–1.5 years and consisted of 8 males and 12 females, which were sexually mature but had not reached adult weights. We created a paraspinal contusion model by percutaneously injecting 10 mL saline into each side of the interspinous ligament (ISL). All segments underwent T2-weighted sagittal and coronal short inversion time inversion recovery (STIR) scans as well as coronal and sagittal 3D proton density-weighted spectrally selective inversion recovery (3D-PDW-SPIR) scans acquired at 1.5T. Following scanning, some ISLs were cut and then the segments were re-scanned using the same magnetic resonance (MR) techniques. Two radiologists independently assessed the MR images, and the reliability of ISL tear interpretation was assessed using the kappa coefficient. The chi-square test was used to compare the diagnostic accuracy of images obtained using the different MR techniques. RESULTS: The interobserver reliability for detecting ISL disruption was high for all imaging techniques (0.776–0.949). The sensitivity, specificity, and diagnostic accuracy of the coronal 3D-PDW-SPIR technique for detecting ISL tears were 100, 96.9, and 97.9%, respectively, which were significantly higher than those of the sagittal STIR (p = 0.000), coronal STIR (p = 0.000), and sagittal 3D-PDW-SPIR (p = 0.001) techniques. CONCLUSION: Compared to other MR methods, coronal 3D-PDW-SPIR provides a more accurate diagnosis of ISL disruption. Adding coronal 3D-PDW-SPIR to a routine MR protocol may help to identify PLC disruptions in cases with nearby contusion.
Adult ; Animals ; Contusions ; Diagnosis ; Female ; Goats ; Humans ; Ligaments ; Magnetic Resonance Imaging ; Male ; Models, Animal ; Protons ; Sensitivity and Specificity ; Spine ; Tears ; Weights and Measures

Objective:To investigate the expression of microRNA-27b (miRNA-27b) , miRNA-221 and miRNA-222 in keloids and their relationship with vascular endothelial growth factor (VEGF).Methods:From January 2021 to January 2022, 36 cases of keloid tissue (keloid group) , 36 cases of normal adjacent scar tissue (normal control group) and 12 cases of flat scar (flat scar group) were selected from Dermatology and Venereology Department of Xinjiang Uygur Autonomous Region People’ s Hospital. VEGF protein expression and microvascular density in the three groups were determined by immunohistochemistry, and the expression levels of miRNA-27b, miRNA-221 and miRNA-222 were detected by real-time quantitative PCR. SPSS 27. 0 statistical software was used for analysis. The measurement data of normal distribution were expressed as Mean ± SD. ANOVA was used for multi-group comparison, and LSD method was used for pound-for-pair multiple comparison. The measurement data of non-normal distribution were expressed as M ( Q1, Q3) , and Kruskal-Wallis H test was used for comparison between groups. Spearman correlation analysis was used for correlation analysis among data. All tests were bilateral tests, and P<0. 05 was considered statistically significant. Results:The levels of VEGF protein and microvascular count in keloid group [(0. 28±0. 08) , (47. 78±14. 06) bar/×400 view] were significantly higher than those in normal control group [(0.09±0.05), (10.25±5.08) bar/×400 view] and flat scar group [(0.19±0.06) , (23.75±7.94) bar/× 400 view] , the difference was statistically significant ( P < 0. 01) . The expression level of miR-27b in keloid group [0.50 (0.32, 0.64) ] was significantly lower than that in normal control group [0.69 (0.37, 1.69) ] and flat scar group [1.35 (0.68, 1.74) ] , the difference was statistically significant ( P<0. 05). There were no significant difference in the expression levels of miR-221 and miR-222 among the three groups ( P>0. 05 ). Correlation analysis showed that miR-27b, miR-221, miR-222 were positively correlated with VEGF protein expression in keloid group ( r=0.36, 0.41, 0.37, P<0. 05). Conclusions:miR-27b, miR-221 and miR-222 in keloid are positively correlated with the expression of VEGF, which may have certain regulatory effects on its angiogenesis, and the low expression of miR-27b may play an important role in the occurrence and development of keloid.

Objective:To investigate the expression of microRNA-27b (miRNA-27b) , miRNA-221 and miRNA-222 in keloids and their relationship with vascular endothelial growth factor (VEGF).Methods:From January 2021 to January 2022, 36 cases of keloid tissue (keloid group) , 36 cases of normal adjacent scar tissue (normal control group) and 12 cases of flat scar (flat scar group) were selected from Dermatology and Venereology Department of Xinjiang Uygur Autonomous Region People’ s Hospital. VEGF protein expression and microvascular density in the three groups were determined by immunohistochemistry, and the expression levels of miRNA-27b, miRNA-221 and miRNA-222 were detected by real-time quantitative PCR. SPSS 27. 0 statistical software was used for analysis. The measurement data of normal distribution were expressed as Mean ± SD. ANOVA was used for multi-group comparison, and LSD method was used for pound-for-pair multiple comparison. The measurement data of non-normal distribution were expressed as M ( Q1, Q3) , and Kruskal-Wallis H test was used for comparison between groups. Spearman correlation analysis was used for correlation analysis among data. All tests were bilateral tests, and P<0. 05 was considered statistically significant. Results:The levels of VEGF protein and microvascular count in keloid group [(0. 28±0. 08) , (47. 78±14. 06) bar/×400 view] were significantly higher than those in normal control group [(0.09±0.05), (10.25±5.08) bar/×400 view] and flat scar group [(0.19±0.06) , (23.75±7.94) bar/× 400 view] , the difference was statistically significant ( P < 0. 01) . The expression level of miR-27b in keloid group [0.50 (0.32, 0.64) ] was significantly lower than that in normal control group [0.69 (0.37, 1.69) ] and flat scar group [1.35 (0.68, 1.74) ] , the difference was statistically significant ( P<0. 05). There were no significant difference in the expression levels of miR-221 and miR-222 among the three groups ( P>0. 05 ). Correlation analysis showed that miR-27b, miR-221, miR-222 were positively correlated with VEGF protein expression in keloid group ( r=0.36, 0.41, 0.37, P<0. 05). Conclusions:miR-27b, miR-221 and miR-222 in keloid are positively correlated with the expression of VEGF, which may have certain regulatory effects on its angiogenesis, and the low expression of miR-27b may play an important role in the occurrence and development of keloid.