OBJECTIVE:To study the improving effect of total flavone from Litchi chinensis (TFL) on the hepatocyte injury in rats with liver fibrosis. METHODS:The rats were given dimethylnitrosamine(DMN),ip,once a day in the first 3 d of every week,which lasted for 30 consecutive days to establish hepatocyte injury model. 60 rats were equally randomized into a normal control(isometric normal saline)group,a model(isometric normal saline)group and the groups of high and low-dose TFL(200 and 100 mg/kg). When the model was being established,drugs were administered,ig,once a day for 45 consecutive days except for normal control group. HE staining was performed,and then the rats’hepatocytes were observed under the microscope and path-ological stage (S1-S4) of liver tissue was analyzed. Masson staining and immunohistochemical staining were conducted,and then the rats’hepatocytes were observed under the microscope and calculation was made for the degree of liver fibrosis and the expres-sion of Bcl-2 and Bax. The activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in rats’serum were determined. RESULTS:The pathological stages of liver cell of rats in the model group were mainly stages S3 and S4 and the groups of high and low doses TFL were dominated by stages S1 and S2. Compared to the rats in the normal control group,those in the mod-el group had higher degree of liver fibrosis,expression of Bcl-2 and Bax and activities of AST and ALT in serum. Compared to the rats in the model group,those in the groups of high and low doses TFL had lower degree of liver fibrosis,higher expression of Bcl-2,lower expression of Bax,and lower activities of AST and ALT in serum. There were statistically significance (P<0.05). CONCLUSIONS:TFL can alleviate the hepatocyte injury in rats with liver fibrosis to some degree by a mechanism which may be related to the up-regulation the expression of Bcl-2 and the down-regulation of the expression of Bax.

Proliferative vitreoretinopathy (PVR)is a serious causing-blindness disease.It is intimately associated with retinal detachment (RD),proliferative diabetic retinopathy (PDR)and penetrating ocular injury with the complicated course and aggravating outcome.Proteomics is a science about investigating the total proteins in cellular level or organism level.The study of proteomics is helpful for reinforcing the cognition and understanding of pathogenesis and development of PVR.So proteomics study about PVR is becoming a new topic.The study progress in the mechanism of PVR,the study background and technique of proteomics,the application of proteomics study in PVR and relative ocular diseases mentioned above are reviewed in this paper.

Helicobacter pylori (H. pylori) is one of the most common pathogens in human and it is closely related to gastrointestinal diseases. It is essential for us to understand the transmission process of H. pylori to prevent its spreading. The oral cavity has been proposed as a reservoir for gastric H. pylori, which has been detected by culture and polymerase chain reaction (PCR) in both dental plaque and saliva. Some researchers have proposed H. pylori in oral cavity may play an important role in its transmission and reinfection. Oral-oral or fecal-oral transmission are thought to be the most possible transmit way. This review will discuss the evidence for the role of the oral cavity in the transmission of H. pylori, the difficulties encountered in addressing this topic and possible directions for future research. Oral H. pylori may also play a role in the diagnosis and prevention of deceases related to H. pylori such as gastritis, gastric ulcer and gastric carcinoma. The recent progresses in this area are also reviewed. Moreover, we also discussed the relationship between oral H. pylori and oral deceases like periodontal disease and oral ulcer.
Dental Plaque ; Gastritis ; Helicobacter Infections ; Helicobacter pylori ; Humans ; Mouth ; Polymerase Chain Reaction ; Saliva

The article analyzed the importance of cultural awareness and cultural self-confidence in stomatology talent training.This article also elaborated on the measure and results of developing three-dimensional teaching system and expanding educational platform and cultural horizons to innovate stomatology talent training based on recent reforms and practice.

Objective:To study the effect of endogenous IL-1? on the expression of matrix metalloproteinases (MMPs) and COX2 in dental pulp cells. Methods:Human dental pulp cells were treated with human recombinant IL-1? at 1 nmol/L in serum-free medium for 18 h. Then the cells were collected and total RNA was isolated, MMPs and COX2 mRNA expression was assessed by semiquantitative RT-PCR. Results:IL-1? at 1 nmol/L induced the expression of COX2 and MMP-1 mRNA in human dental pulp cells. Conclusion:IL-1? may contribute to stimulating expression of MMPs and COX2 in the dental pulp during pulpitis.

Objective:To determine the effects of TGF-?1 on the cytoskeleton in cultured human dental pulp cells(HPCs).Methods:Human dental pulp cells were cultured from dental pulp tissue explants digested with collagenase I. Semi-confluent cultures of the cells maintained under serum deprivation were treated with 20 ng/ml of TGF-?1 for 30 min, 1, 6 and 24 h respectively.Then cells were processed for BODYPY-phalloidin direct fluorescence examination of the actin filaments,DAPI direct fluorescence of the nucleus and Rhodamine Red(TM) indirect immunofluorescence of tubulin-?. Confocal laser scanning microscopy was used to investigate the changes of actin filaments and microtubules.Results:Disintegration and reorganization of actin filaments were observed in human dental pulp cells treated by TGF-?1 at 20 ng/ml. Actin filaments assembly was found near the cell membrane,specially after 30 min exposure.Disintegration of actin filaments was most obvious after 6 h treatment.Actin filaments were reorganized after 24 h exposure.Microtubules mainly remained intact in the cells during TGF-?1 treatment.Conclusion:TGF-?1 at 20 ng/ml may induce actin cytoskeletal reorganization in human dental pulp cells.

Objective: To investigate the inhibitory effect of Flu or Protector on the demineralization of bovine enamel caused by some beverages. Methods: Forty bovine teeth were equally divided into experimental and control groups, each of them were subdivided into two groups. The enamel sp ecimens of the experimental group were pretreated with Fluor Protector and then exposed to carbonated drinks and fruit juice respectively,the enamel specimens o f control group were exposed to beverages directly. All the enamel specimens wer e exposed to beverages 5 times a day and for 5 minutes each time. After 7- day exposure,the concentration of calcium and phosphate in the beverages were measu red by EL 312e Micro-Plate. Results: The concentration of calci um and phosphate in the corresponding beverage of experimental group was lower t han that in control group in the same expossure time(P0.05).Conclusions: Fluor Protector can inhibit the demineralization of bovin e enamel caused by beverages.The inhibitory effect of Fluor Protector decreases as the exposure time prolonges.

Objective: To study the effects of ureolysis on the proliferation and acid-resistance of Actinomyces naeslundii.Methods:The growth of Actinomyces naeslundii cultured in different nitrogen sources were measured, and the acid-resistant ability of Actinomyces naeslundii cultured in acid condition with different urea concentration was compared.Results:In contrast with other nitrogen sources,Actinomyces naeslundii could be cultured by urea and higher final A value of the bacteria was obtained;at the range of pH=4.0-7.0, ureolysis could increase the survival of Actinomyces naeslundii from acid killing to some extend, but at pH=3.0, ureolysis could not protect Actinomyces naeslundii anymore.Conclusion:Ureolysis may stimulate the growth of A. naeslundii and increase the acid-resistant ability of Actinomyces naeslundii.

Objective: To assay minimal biofilm eradication concentration (MBEC) of various extraction compounds from Galla Chinensis to 5 oral bacterial biofilms, and then to set up traditional Chinese medicine empirical study methods of pharmacodynamic susceptibility. Methods:5 cariogenic bacteria strains were selected (Streptococcus mutans ATCC25175, Streptococcus sanguis ATCC10556, Lactobacillus rhamnosus AC413, Actinomyces naeslundii WVU627, Actinomyces viscosus ATCC19246) in present study. Various extraction compounds of Galla Chinensis were GCE (aqueous extract), GCE-B(300 ml/L alcohol extract), GCE-C(500 ml/L acetone extract),GCE-D(1 000 ml/L acetone extract), gallic acid and methyl gallate. ①Observed oral bacterial biofilm formation in MBECTM-Device at different-time-stages. ②MBECTM -HTP-Assay:The minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) of various extraction compounds of Galla Chinensis on 5 oral bacteria strains were determined. Results:5 oral bacterial biofilm were readily formed on the lid of MBECTM-Device under selected condition (observed by SEM). 5 oral cariogenic bacteria growing as planktonic populations were sensitive to 6 extraction compounds of Galla Chinensis. When compared to 6 extraction compounds of Galla Chinensis, 5 oral cariogenic bacterial biofilm were 2-16 times less susceptible than growing planktonic bacteria. GCE and GCE-B are the most effective medicine against 5 oral cariogenic bacterial biofilm. GCE-C and GCE-D were effective in killing oral-bacterial biofilm at relatively high concentration, but gallic acid and methyl gallate were less effective.Conclusion:GCE and GCE-B are the most effective medicine against 5 oral cariogenic bacterial biofilm of 6 extraction compounds from Galla Chinensis. MBEC can provide a relatively accurate medicine concentration for clinical test.

A human oral microbiota is the ecological community of commensal, symbiotic, and pathogenic microorganisms found in human oral cavity. Oral microbiota exists mostly in the form of a biofilm and maintains a dynamic ecological equilibrium with the host body. However, the disturbance of this ecological balance inevitably causes oral infectious diseases, such as dental caries, apical periodontitis, periodontal diseases, pericoronitis, and craniofacial bone osteomyelitis. Oral microbiota is also correlated with many systemic diseases, including cancer, diabetes mellitus, rheumatoid arthritis, cardiovascular diseases, and preterm birth. Hence, oral microbiota has been considered as a potential biomarker of human diseases. The "Human Microbiome Project" and other metagenomic projects worldwide have advanced our knowledge of the human oral microbiota. The integration of these metadata has been the frontier of oral microbiology to improve clinical translation. By reviewing recent progress on studies involving oral microbiota-related oral and systemic diseases, we aimed to propose the essential role of oral microbiota in the prediction of the onset, progression, and prognosis of oral and systemic diseases. An oral microbiota-based prediction model helps develop a new paradigm of personalized medicine and benefits the human health in the post-metagenomics era.
Biomarkers ; Cardiovascular Diseases ; microbiology ; Dental Caries ; microbiology ; Diabetes Mellitus ; microbiology ; Humans ; Metagenomics ; Microbiota ; Mouth ; microbiology ; Mouth Diseases ; microbiology ; Neoplasms ; microbiology ; Oral Health ; Periodontal Diseases ; microbiology