Objective To investigate the effects of polysaccharide from Spirulina platensis (PSP) on the growth of HeLa cells. Methods The antiproliferation activities of PSP on HeLa cells were evaluated by MTT assay. The morphologic changes of HeLa cells were observed under light microscope. Cells division cycle was measured by flow cytometry. Results It was showed that with the increase of PSP concentration, the inhibited rate on HeLa cells increased and the survived rate declined; The HeLa cells exposed to PSP for 24～48h displayed significant morphologic changes; PSP had a specific effect on the HeLa cell division cycle, increased the number of cells in phase G 1. Conclusion These results indicated that the effects of PSP on HeLa cells may probably be attributed to increase of the number of cells in phase G 1.

Industrial culture media for cultivation of marine microalgae Nannochloropsis oculata was optimized by using Random Design software. High eicosapentaenoic acid (EPA) content was obtained over 40% of total fatty acid, and high average specific growth rate of 0.32d -1 was obtained in cells grown in the selected Formula 1. Mass cultivation of this species was practiced in indoor open raceway ponds of 6.12 m 2 and 28.02 m 2, and in outdoor open pond of 83.50 m 2 during May and June in 1999. The average area yield of biomass were obtained at 2.77, 1.62 and 2.52 g /m 2?d, and in October, 1.09 g/ m 2?d was obtained in 83.5m 2 outdoor pond, and the harvest algae powder contained 49.50% of protein, 20.30% of total lipid and 3.00% of EPA of dry weight. With high temperature tolerant species during August in 2000, the average area yield of biomass were increased to 5.44?9.96 and 7.66 g/ m 2?d in above ponds, respectively and 2.50% of EPA of dry weight was contained in the powder. It is indicated that the industrialized cultivation of Nannochloropsis oculata rich in EPA is completely doable on practice, and the algal powder was a good raw material as single cell protein and development of EPA rich products.

Objective Effects of fucoidan from Cladosiphon okamuranusa (CF)on mice bearing Siso were investigated. Methods Different concentrations of CF were given to mice bearing tumor by ig. The tumor weight, spleen index, thymus index and serum MDA content, serum SOD activity were detected by physical and biochemical methods. Results The growth of S180 tumor was inhibited significantly by CF in different concentrations. The inhibitory rate ranged from 27. 83% to 33. 97%. The highest inhibition rate occurred in the medium dosage. The spleen index, thymus index and leukocyte counts were not influenced obviously. The content of serum MDA was decreased remarkably and the activity of serum SOD was enhanced notably. Conclusion The mechanism of anti -tumor effects of CF may relate with its antioxidation.

BACKGROUND:Traumatic soft tissue defects of the limbs are usual y accompanied with the exposure of tendon, joint capsule, bone or internal fixator, which can be reconstructed by skin flap. Previous research has shown that it is an important method to repair traumatic tissue defects with flaps. However, rarely research reports perioperative management about flap bed so far. OBJECTIVE:To explore the perioperative strategy for repairing traumatic soft tissue defects with revascularized flaps. METHODS:Total y 94 cases undergoing secondary skin flap repair were enrol ed. Intraoperative debridement using tourniquet was performed, and the wound was washed with mass of physiological saline. Whether the tissues, including bone, tendon, joint capsule and internal fixator, were reserved or not depended on their viability, and then the flaps were harvested to repair defects, and drainage was placed properly at last. RESULTS AND CONCLUSION:The flaps survived in al cases. Exudation occurred in 5 cases with the exposure of bone, and 28 cases with the exposure of tendon or joint capsule healed normal y. No complications were associated with the reservation of the internal fixators, but delayed-union occurred in three cases and nonunion in one case. These findings indicate that the perioperative treatment of the application of skin flap is worthy of attention. Careful debridement, advisable choice of the flap, efficient drainage and using antibiotic normatively are al keys. Treatment of the bone, tendon, joint capsule and internal fixator which are exposed should not only weigh the advantage and disadvantage, but also relax the indication of reserving them.

AIM: Using surface active agent that has wetting ability and solubilising to increase the extraction rate of curcumin. METHODS: Different surface active agents, such as span, span 60, tween 20, tween 85. SDS and CSBS, were added and then the curcumin was determined. RESULTS: When we added 0.5% sodium dodecyl sulfate (SDS), it could increase the extractive rate of curcumin by 16%. CONCLUSION: Different surface active agents have variable solubiitzation of different drugs. SDS can affect the extractive rate of curcumin.

Objective To study the causes, diagnosis and treatment of the spontaneous perforation of sigmoid colon(SPSC). Methods The clinical data of 11 patients with SPSC admitted into our hospital from January. 1984 to September. 2000 were analyzed retrospectively. Results All the 11 cases of SPSC were proved by operation. Among them, 4 cases had chronic constipation history, 1 case complicated with mild proctoptoma before operation and the other 6 cases had no special records. 9 cases were misdiagnosed as perforation of acute appendicitis, perforation of upper gastroenteric tract, perforation of colon carcinoma, inflammatory perforation or fecal mass perforation. 2 cases recovered after repairing operation; of 9 cases underwent colostomy and other procedure, 7 cases recovered and colostomy closure was done 2-3 months after operation. 2 cases died after operation. Conclusions The occurrence of SPSC is closely related to the configuration and position of the patient's sigmoid colon. Increase of intra-abdominal pressure and intra-intestinal pressure are the predisposing causes of the disease. This disease has no special clinical manifestation. The key to diagnosis is a full knwledge of the disease before operation. The main treatment is operation. The mode of operation is determined according to patient's age, general condition, severity of abdominal pollution, attacking time. The best mode of operation is to repair and close the perforation or to resect and anastomose the bowel and colostomy.

Objective To investigate the effect of gavaged yAGA2-sCT, a recombinant Saccharomyces cerevisiae which expressed salmon calcitonin on its surface, on serum calcium concentration of rats. Methods The sCT gene was artificially synthesized in advance and cloned into the pYD1 vector, a kind of surface displaying plasmid, to yield pYD1-sCT. The recombinant plasmid pYD1-sCT was transformed into S. cerevisiae EBY100 by LiAC method and achieved yAGA2-sCT. The sCT protein, which was expressed on the surface of recombinant S. cerevisiae yAGA2-sCT, was indirectly labeled with FITC. Labeled yeast was immediately detected under the fluorescent microscope. Before being gavaged to rats, recombinant yeast was frozen and vacuum-dried. Lyophilized yeast yAGA2-sCT was administered to rats in dosage of 0.1g/kg, 0.5g/kg and 5.0g/kg, respectively, with 6 rats in each group. Positive drug group (200mU/kg calcitonin via hypodermic injection) and two conditional control groups (being gavaged 5g/kg yAGA2-sCT and 30?g/kg calcitonin, respectively) were acted as controls. The serum was collected from canthus venous plexus and the calcium concentration was immediately measured by automatic biochemical analyzer. Results The sCT protein was successfully expressed on the surface of recombinant yAGA2-sCT, and FITC-labeled yAGA2-sCT was observed under the fluorescent microscope. The recombinant S. cerevisiae yAGA2-sCT was able to reduce the serum calcium concentration of rats in a dose-dependent manner. In the three subgroups, the hypocalcemic effect of high-dosage subgroup, with 5g/kg lyophilized yAGA2-sCT, was the highest (P

We innovated video assessment integrated teaching method on the basis of clinical case discussion,teaching rounds,problem-based learning,case method and micro teaching.According to this video feedback teaching,students became teaching subject and teacher acted as guide.In order to realize the purposes of early contact with clinical practice,more clinical practice and repeated clinical practice teaching,we implemented student-centered small class teaching,which was heuristic,inquisitive,interactive and was discussion-based.At the same time with the help of medical simulation equipment and video feedback teaching,this method developed students' life-long learning ability and work competence and combined theory with practice,demonstration with imitation as well as standards with innovation.This method was not only suitable for theoretical study but also for skills training,especially for standardization training.

0.05). Regarding to the grade of irradiation mucositis, the majority of patients in the treatment group were at stage Ⅰ-Ⅱ,while those in the control at stage Ⅲ-Ⅳ(P0.05).No adverse drug reaction was observed in both groups during irradiation process.Conclusion:Actovegin can postpone the development of irradiation oropharyngeal mucositis and decrease the incidence of gradeⅢ and Ⅳmucositis.

Objective To study the impact of capitation payment on obstetric indicators for the maternity insurance of urban workers in Yinchuan city.Methods Collection of indicators on lying-in women hospitalized at the obstetrics departments of designated hospitals in Yinchuan,in the period of 2011 to 2012 when the capitation payment was put in place.Such indicators include the percentage of uterine-incision delivery,diagnostics and therapeutic expenses,drug expenses,and average days of stay,along with mortality of pregnant and lying-in women and that of newborns,which are used as indicators to measure quality of care.Results The capitation payment policy has witnessed drops in the percentage of uterine-incision delivery,cost per inpatient,drug expenses per inpatient and average days of stay among urban workers covered by the insurance.The drops amount to 10％ for cost per inpatient and 45％ for drug expenses per inpatient.The differences found in pregnant and lying-in women are not statistically significant.Conclusion Capitation payment is conducive to dropping the percentage of uterine-incision delivery and medical expenses,and saving medical insurance payment,for the sake of optimal use of healthcare resources.