OBJECTIVE: To investigate the effects of carvedilol on blood pressure in hypertension patients and vascular endothelial function. METHODS: 100 inpatients with primary hypertension were collected from our hospital from Jan. 2008 to Jan. 2010 and randomly divided into treatment group and control group(n=50). Treatment group were given initial oral dose of carvedilol tablets 6.25 mg twice a day for 7～14 days if it was tolerated; the dose of carvedilol tablets increased to 12.5 mg or 25 mg twice a day according to blood pressure at trough concentration if it was necessary. Control group were given carvedilol tablets 6.25 mg twice a day if it was tolerated. After 1 week, control group received double dose of metoprolol with maximum dose of carvedilol of 50 mg. The treatment course was 3 months. The contents of NO and ET in serum of 2 groups were detected. Antihypertensive effect and effects of carvedilol on vascular endothelial dilatation and systolic function were observed and compared between 2 groups. RESULTS: After treatment, the systolic and diastolic blood pressure in both groups were decreased significantly(P

Objective To investigate the role of correcting anemia in improving the heart function of chronic heart failure(CHF) with anemia. Methods Sixty-six CHF patients with anemia were randomly assigned to two groups. Thirty cases in the control group received routine treatment including digitalis, diuretics, vasodilator,ACEI or β-blocker,and 36 cases in the observe group received the above routine treatment and EPO,chalybeate and transferring hematid. Results Clinical efficiency ratio and total efficiency ratio were higher in the observe group (63. 9% ,91.7%) than in the control group(33.3% ,56. 7%) (χ2 =6. 73 and 10.91 ,P ＜0.01). After treatment for 3 months,Hb concentration, LVEF, LVDD, LEDV and LESV in the observe group were (125.0 ± 4. 0) g/L,(49.6±8.0)%,(4.9 ±0.7) cm,(130.1 ±24.0) ml and (72.5 ±32.0) ml respectively,which were all significantly improved than those in the control group [(80.0 ± 7.0) g/L, (34. 7 ± 10.0) %, (6. 0 ± 0.4) cm,(148. 3 ± 30.8) ml and (79. 7 ± 25. 0) ml] (P ＜ 0.01). Conclusions Correcting anemia is a safe and effective method in improveing the heart functionin CHF patients with anemia.

Objective: To investigate prevalence of neurosis in a college of central China. Method:4465 college students were investigated by epidemiological method used in the national survey of 12 areas 10 years before. Result: The present prevalence of neurosis in students were 9.34%, higher than that of the national survey 10 years before (6.44%). In different diagnoses, the present prevalence of anxiety disorder was much higher. Male students had higher rate of neuroses than female, those in grade 3 or 4 had higher rate of neuroses than those in other grades. The most obvious invoke factor of neurosis was lovelorn. Conclusion: The prevalence of neurosis is higher than 10 years before.

Objective To study the role of heparin-binding protein (HBP) in sepsis-associated acute respiratory distress syndrome (ARDS),and to evaluate the prognostic value of HBP in ARDS.Method Sixty seven sepsis patients were enrolled in the prospective study.According to whether present ARDS,patients were divided into two groups:ARDS group and non-ARDS group.Blood samples were obtained within 2 hours after patients were diagnosed with sepsis.We measured the level of interleukin-6,interleukin8 and HBP by ELISA,counted the number of polymorphonuclear neutrophils (PMN),and calculated Acute Physiology and Chronic Health Evaluation Ⅱ score.We compared interleukin-6,interleukin-8,PMN and HBP between two groups by Student's t test.Correlation analysis was studied between HBP and other indicators by Spearman rank correlation coefficient.We also studied the early prognostic value on sepsisassociated ARDS by establishing receiver operating characteristic (ROC) curve.Results Interleukin-6 (t=4.25,P＜0.01),interleukin-8 (t=10.10,P＜0.01),PMN (t=3.47,P=0.0009) and HBP (t =0.0225,P =2.336,all showed significant difference between ARDS group and non-ARDS group.Interleukin-6 (r=0.535,P=0.002),interleukin-8 (r=0.419,P=0.017) and PMN (r=0.419,P =0.017) all had positive correlation with HBP.The area under curve (AUC) of HBP predicting ARDS was 0.796.Conclusions HBP level elevated in sepsis-associated ARDS patients and were correlated with interleuki-6,interleuki-8 and PMN.HBP show a high value on predicting emergence of sepsis-associated ARDS.HBP may play an important role in sepsis-associated ARDS pathological process.From this study we can conclude one of possible ARDS pathogenesis:inflammatory mediators,such as interleuki-6,interleuki8,activate PMN.Subseqoently PMN releases HBP.Then HBP resolts in vascolar leakage,which is one of the basic ARDS pathology.

M-waves and F-responses were investigated before and after massage using the bipolar surface electrodes to stimulate the tibial nerve at the ankle recorded by bipolar surface electrodes from the muscle flexor hallucis in 30 children with cerebral palsy. After treatment, the amplitudes of the M-wave increased (P＜0.05 ); F-response reduced (P＜0.05 )in the lesion side. The observations were made bilaterally and the results revealed no significant differences(P＞0.05 )by comparing side to side. The latencies of Mwaves prolonged to 6.09～8. 28ms(P<0.05);F-responses 29.07～3l. 42ms(only Fn wave was P＜0.05in the lesion side). The significant efficiency was 25.93 %,the efficiency was 88.89 % except that 2 objects did not change and one continued to deteriorate. The experimental results suggest that the massage can improve the functional disorder of the skeletal muscle and the ventral horn of the spinal cord.

Objective To investigate the relationship between chronic prostatitis (CP) and male infertility. Methods 120 cases of male infertility patients with chronic prostatitis and 120 cases of male infertility patients with-out chronic prostatitis were analyzed. Results The differences of sperm quality、sperm movement function, partial bio-chemical indicator in semen and sperm aggregation in the two groups are significant (P<0.05). Conclusion CP plays an important negative role in male infertility.

OBJECTIVE To explore the NG rapid and accurate detection method,epidemiological characteristics and the control of nosocomical infection.METHODS From 1989 to 2008,46 898 patients were under taken the NG smear test,culture and NG immune latex test.RESULTS In the 46 898 cases,764 cases were found out G-diplococci both inside and outside cells or cell.The positive rate was 1.63%.In 764 positive samples,721 cases were not found out other bacteria(94.37%),NG positive smears had their own features.CONCLUSIONS When no bacteria growth and a large number of swollen neutrophils,particular attention should be paid.NG smears particularly for small media-size community and rural hospitals,without NG culture,PCR and NG antigen/antibody test can be choosed first.

Objective To evaluate the efficacy and safety of DA-EPOCH-R protocol for patients with B-cell non-hodgkin lymphoma (NHL). Methods 43 patients with B-cell NHL received DA-EPOCH-R protocol, and their efficacy and adverse reactions were analyzed. Results 43 patients received a total of 203 cycles of chemotherapy and the median chemotherapy cycle was 6 (2ˉ8 cycles). 32 patients (74.4%) achieved complete remission (CR) after 2ˉ4 cycles of chemotherapy. A further analysis found that age ≤60 years and>60 years, stageⅠ/Ⅱand stageⅢ/Ⅳ, germinal center B-cell (GCB), non-GCB, double expression lymphoma (DEL) and non-DEL patients had no significant differences (P> 0.05). With a median follow-up of 40 months (9ˉ62 mouths), the overall survival (OS) rate of 1-year and 3-year was 97.6 % and 92.8 % respectively. The major toxicity of DA-EPOCH-R protocol was hematologic toxicity. Other toxicities were mild, and no treatment-related deaths occurred. At the end of follow-up, no secondary tumors were found. Conclusions DA-EPOCH-R protocol is an effective and safe protocol for patients with NHL. The result shows that the curative effect of patients in stageⅢandⅣis similar to the patients in stageⅠandⅡ.

A group of 19 referred hypertensive patients were diagnosed to have primary aldosteronism(PA) with inconclusive computed tomography scan results.Adrenal vein sarmpling (AVS) was performed in all patients.AVS was successful in 16 cases but failed in 3 cases.According to the results of AVS and postoperative pathology,8 cases were diagnosed as aldosterone-producing adenoma (APA) and unilateral adrenal hyperplasia (UAH),and the other 8 cases were diagnosed as idiopathic hyperaldosteronism (IHA).In conclusion,AVS is one of the most crucial methods in typing diagnosis of PA.

Objective To construct eukaryotic Flag (DYKDDDDK) expressing recombinant plasmids, pCMV-N-Flag-SND1-No1/2, which contain the coding sequence of human SND1-No1(from 1st AUG)or SND1-No2 (from 2nd AUG), and perform the cellular localization analysis of Flag-tagged SND1-No1/2 under stress condition to study the function of the two AUG in the SND1 containing stress granules formation. Methods The gene fragments of SND1-No1/2 were amplified by PCR from the whole SND1 transcript and inserted into pCMV-N-Flag expressing vector through BamHI/EcoRI double en-zyme digestion and T4 DNA Ligase connection. The recombinant pCMV-N-Flag-SND1-No1/2 plasmids were transfected in-to HeLa cells and the expression of Flag-SND1-No1/2 fusion proteins was examined by Western blotting assay. Immunofluo-rescence assay was performed to detect the co-localization of Flag-SND1-No1/2 with endogenous SND1 granule. Results The pCMV-N-Flag-SND1-No1/2 were sequenced and digested correctly by restriction single/double enzyme. The Flag-tagged SND1-No1/2 fusion proteins were also detected in transfected HeLa cell by Western blotting assay. Both of them showed the co-localization with endogenous SND1 granule. Conclusion The recombinant eukaryotic plasmids of pCMV-N-Flag-SND1-No1/2 were constructed successfully and expressed effectively. The depletion of 1st AUG failed to af-fect the formation of SND1 containing stress granules.