Objective To investigate the time course of nuclear factor-κB(NF-κB) and the effects of pyrrolidine dithiocarbamate (PDTC) on the expressions of NF-κB,tumor necrosis factor-α (TNF-α) and interleukin1β(IL-1β) in hippocampus after seizures.Methods Epilepsy were induced by [PTZ] through Intraperitoneal injection.Western blotting was used to detect NF-κB p65 expression in nucleus at various experiment groups in different time points( 14d,21 d,28d,35d).Moreover,mRNA and protein expressions of TNF-α and IL-1β in different experiment groups in different time points by Real-time quantitative PCR and ELISA analysis.Results The expression of NF-κB p65 began to increase in the nuclear fraction in 14d,kept rising in 28d and returned to 14d level in 35d after epilepsy seizures,At 14d,21d,28d and 35d,the expressions of NF-κB in PDTC groups ( (0.54 ±0.07),(0.65 ± 0.08 ),(0.78 ± 0.10),(0.78 ± 0.10) ) was significantly lower than those in PTZ groups ((1.20 ±0.11),(1.42 ±0.14),(1.88 ±0.16),(1.25 ±0.10)) (P＜0.01).After epilepsy seizures,the expression of TNF-α 、IL-1β mRNA was increased in PTZ groups( ( 1.34 ±0.13,0.81 ± O.17 ),( 1.64 ±0.17,1.56±0.20),(2.03 ±0.16,1.65 ±0.18),(1.40 ±0.10,1.30 ±0.13) ) than those in NS groups(P＜0.01 ) ;and compared with PTZ groups PDTC significantly decrease the mRNA expressions of TNF-α and IL-1 β in PDTC groups( (0.96 ±0.1,0.57 ±0.07),(1.36 ±0.15,1.09 ±0.18),(1.47 ±0.14,1.25 ±0.16),(1.12 ±0.12,O.85 ± 0.12) ) (P ＜ 0.05 ).The expressions of TN F-α,IL-1β protein were similar in hippocampal by ELISA.Conclusion Seizures induces NF-κB nucleus translocation and promotes the expressions of TNF-ot and IL-1 β in hippocampus and pyrrolidine dithiocarbamate suppresses NF-κB associated inflammatory pathway in epileptic rat hippocampus.

Objective To evaluate CT-guided pereutaneous biopsy and fine needle aspiration in diagnosing malignant musculoskeletal tumors. Methods CT-guided percutaneous biopsy and fine needle aspiration was performed in 106 cases with suspected musculoskeletal tumor. The pathological results obtained from biopsy specimens were compared with clinical final pathological diagnoses. Results All 106 cases were finally diagnosed as suffering from malignant musculoskeletal tumors, which were proved by operation and incisional biopsy. Of 106 cases receiving CT-guided percutaneous biopsy and needle aspiration, the pathologic diagnosis obtained from this procedure was consistent with the clinical final pathological diagnosis in 89 and was "negative" in 17, with an accuracy rate of 84.0%. Conclusion CT-guided percutaneous biopsy and fine needle aspiration is a safe, simple and effective technique for the diagnosis of malignant musculoskeletal tumors, Incisional biopsy may be necessary when this procedure gives "negative" result.

Objective: To explore the risk factors in patients with chronic heart failure (CHF) combining atrial ifbrillation (AF) and to identify the predictive factors for AF recurrence after cardio version. Methods: A total of 246 CHF patients treated in our hospital were divided into 2 groups: CHF+AF (AF) group,n=71 and CHF without AF (Non-AF) group,n=175. The baseline information was recorded. The risk factors for AF occurrence were screened by Logistic regression analysis. There were 66 AF patients with successful cardio verse and they were followed-up for 18 months, the predictors for AF recurrence were studied by Cox regression analysis. Results: The baseline information showed that age, CHF duration, left atria diameter (LAD), the levels of BNP, uric acid and serum creatinine in AF group were all higher than those in Non-AF group, allP<0.05. Logistic regression analysis presented that age (RR=1.89, 95% CI 1.08-3.31,P<0.05), CHF duration (RR=1.35, 95% CI 1.05-1.74,P<0.05), BNP level (RR=1.82, 95% CI 1.30-2.53,P<0.01), LAD (RR=1.51, 95% CI 1.09-2.08,P<0.05) were the risk factors for AF occurrence. Cox regression analysis indicated that BNP level (RR=1.30, 95% CI 1.09-1.54,P<0.01) and LAD (RR=1.74, 95% CI 1.30-2.34, P<0.01) were the predictors for AF recurrence. Conclusion: The elder age, longer CHF duration, increased BNP level and LAD were the risk factors for AF occurrence in CHF patients. The increased BNP level and LAD were the objective predictors for AF recurrence after cardio version.

OBJECTIVE:To evaluate the economic value of Shenqi fuzheng injection combined with chemotherapy vs. chemo-therapy alone for non-small lung cancer(NSCLC),and to provide reference for drug payment and clinical treatment. METHODS:By prospective cohort design,516 patients with non-small cell lung cancer from 11 hospitals were selected as subjects according to the sale distribution of Shenqi fuzheng injection in district and hospital;267 cases of Shenqi fuzheng injection combined with che-motherapy and 249 cases of chemotherapy alone formed naturally according the clinical therapy plan. The cost-effectiveness and cost-utility analysis were conducted using FACT-L score,KPS score and ZPS score as effect index,QALYs as effectiveness index observation period of 21 d. RESULTS:5 dimensions FACT-L score:the patients of Shenqi fuzheng injection combined with chemo-therapy group could be effectively improved,and the cost-effectiveness ratio was lower than chemotherapy alone group;KPS score:the cost-effectiveness ratio of Shenqi fuzheng injection combined with chemotherapy group was lower than chemotherapy alone group (1 632.44 vs. 11 145.30),and incremental cost-effectiveness ratio was 448.69. ZPS score:Shenqi fuzheng injection combined with chemotherapy group was lower than chemotherapy alone(-17 398.77 vs. 384 513.00). Shenqi fuzheng injection combined with chemotherapy group was lower than chemotherapy alone group in average cost per 1 QALYs(1 313 326 yuan vs. 13 374 365 yuan). CONCLUSIONS:Compared with chemotherapy alone group,Shenqi fuzheng injection combined with chemo-therapy can effectively improve the quality of life,and it is worth of spending more money on Shenqi fuzheng injection from the perspective of the pharmacoeconomics.

Objective To evaluate CT-guided percutaneous biopsy and fine needle aspiration in diagnosing malignant musculoskeletal tumors.Methods CT-guided percutaneous biopsy and fine needle aspiration was performed in 106 cases with suspected musculoskeletal tumor.The pathological results obtained from biopsy specimens were compared with clinical final pathological diagnoses.Results All 106 cases were finally diagnosed as suffering from malignant musculoskeletal tumors,which were proved by operation and incisional biopsy.Of 106 cases receiving CT-guided percutaneous biopsy and needle aspiration,the pathologic diagnosis obtained from this procedure was consistent with the clinical final pathological diagnosis in 89 and was "negative" in 17,with an accuracy rate of 84.0%.Conclusion CT-guided percutaneous biopsy and fine needle aspiration is a safe,simple and effective technique for the diagnosis of malignant musculoskeletal tumors.Incisional biopsy may be necessary when this procedure gives "negative" result.

Objective To discuss the distribution of traditional Chinese medicine (TCM) syndromes of patients with congestive heart failure (CHF) commonly seen in clinic based on data mining technology.Methods A multicenter study was conducted. 1 451 in-patients with CHF in 4 grade Ⅲ hospitals from December 2010 to September 2012 were enrolled. The cluster and factor methods were applied to synthetically analyze the data concerning the patients' information of four diagnostic methods in TCM, and the TCM syndromes commonly seen in patients with CHF and the preliminary diagnostic scheme were summarized.Results In these 1 451 cases, there were 43 items having been experienced standardization, including clinical symptoms and manifestations of tongue and pulse, and in the information concerning four diagnosis in TCM, there were 14 items whose incidences > 10%. The incidence of chest distress was the highest 89.18% (1 294 cases), followed by shortness of breath 66.16% (960 cases), and the lowest constipation 12.20% (177 cases). There were 4 commonly seen syndromes of CHF as follows: qi deficiency blood stasis syndrome (the major diagnostic bases: palpitation, shortness of breath, dark and red tongue with white fur, thin pulse; and the minor bases: chest distress and thin fur), yang deficiency water diffusion syndrome (major bases: edema of lower limbs, cough, expectoration, difficult urination, chilly, poor appetite, greasy fur, string pulse; minor bases: body strength weakness), phlegm heat obstructing lung syndrome (major bases: chest pain, cough and dyspnea in semi-reclining position, constipation, light tongue with yellow fur, deep, slippery or rapid pulse; minor bases: dizziness), and qi yin deficiency blood stasis syndrome (major bases: dry mouth, night sweating, frequent urination at night, teeth-marked tongue, purplish tongue, uneven or irregular pulse; minor bases: loose stool, headache, purplish lips, swollen tongue with little fur, moderate or weak pulse). The load intervals of each of the symptoms in CHF qi deficiency blood stasis syndrome were from -0.520 to 0.881, all the absolute values were greater than 0.520, and the common degrees extracted were 64.3% - 83.6%, which meant the 5 common factors could extract the original index information all over the common degree 64.3%. In yang deficiency water diffusion syndrome, the load intervals of each of symptoms were from0.732 to 0.983, all the absolute values were greater than 0.732, and the common degrees extracted were 58.2% - 96.9%; in phlegm heat obstructing lung syndrome, the load intervals of each of symptoms were 0.670 - 0.992, the all absolute values were greater than 0.670, and the common degrees extracted were 52.0% - 98.5%. In qi yin deficiency blood stasis syndrome, the load intervals of each of symptoms were 0.576 - 0.926, all the absolute values were greater than 0.576, and the common degrees extracted were 54.2% - 87.1%.Conclusion By application of data mining technology combined with clinical practice, the common TCM syndromes of patients with CHF can be analyzed objectively and comprehensively.

Objective To explore the clinical significance of cerebrospinal fluid neuropeptide Y in epileptic patients with intelligence disturbance.Methods The Full Intelligence Quotient (FIQ) of 78 cases of epileptic patients were assessed by WAIS-RC.The subjects were divided into intelligence disturbance group and non-intelligence disturbance group.Their cerebrospinal fluid neuropeptide Y Was tested by using radio immunoassay.Results The content of neuropeptide Y in intelligence disturbance group Was obviously higher than that in non-intelligence disturbance group (P<0.01).Conclusion Intelligence disturbance in epileptic patients is related to the increased Neuropeptide Y.Neuropeptide Y may reflect the intelligence condition in epileptic patients.

Objective To study the expressions of erythmpoietin(EPO)and its receptors(EPOR)in the injured brain tissue ofthe rats with traumatic brain injury(TBI).Methods A total of78 SD rats were randomly divided into three groups including control group(six rats),sham group(36rats) and fluid percussion injury group(36 rats).The rats were sacrificed at 6,24 hours,3,5,7 and 14days after TBI in the sham group and the fluid percussion injury group(six rats at each time point).Then,the injured brain tissues were removed for observation of the mRNA and protein expressions of EPO and EPOR by meaDiB of real-time PCR and Western blot. Results The expression of EPO was increased at 24 hours and reached the peak at day 3 after TBI.The hish expression level of EPO could maintain for two days or so.began to decrease at day 7 and recovered to normal at day 14 after Till.While the expression of EPOR reached the peak at 24 hours after TBI and maintained hish level at day14. Conclusions The expressions of EPO and EPOR show increase within 24 hours after TBI.In fact,the expressions of both factors are not in consistency,with more transient expression of EPO.

Objective To investigate the relationship between chronic prostatitis (CP) and male infertility. Methods 120 cases of male infertility patients with chronic prostatitis and 120 cases of male infertility patients with-out chronic prostatitis were analyzed. Results The differences of sperm quality、sperm movement function, partial bio-chemical indicator in semen and sperm aggregation in the two groups are significant (P<0.05). Conclusion CP plays an important negative role in male infertility.

Objective To investigate the frequency of lesions detection in patients with cerebral infarction (CI) with SPECT/CT. To investigate fluctuation of regional cerebral blood flow (rCBF) and its relationship with clinical symptoms. Methods Sixty-seven CI patients without cerebellar lesion were randomly selected. The rCBF in the regions of interest (ROI) was examined by SPECT/CT, which was collected from the frontal lobe, temporal lobe, parietal lobe, occipital lobe, thalamus, basal ganglia and cerebellum. The rCBF index was calculated. The association between fluctuation of rCBF index and clinical symptoms of patients was explored. Results There were 251 positive regions in all viewing regions , the total positive rate was 31.2%. The left side was 38.1%, while the right side was 24.4% (χ2=17.522,P < 0.01). In normal group, there were no statistical difference of average rCBF between two halves (P > 0.05). However, the average rCBF on the left parietal lobe was lower (P < 0.01). The average rCBF in the abnormal group was lower than that in ipsilateral normal group (P < 0.01). The average rCBF index in the abnormal group was higher (P < 0.01). In normal group , the average rCBF on the frontal lobe and parietal lobe was low , but the average rCBF on the thalamus and basal ganglia was high (P < 0.01). In abnormal group, there were no statistical difference in the average rCBF (P >0.05). rCBF≥0.7 is a clinical sign of abnormal ROI. Conclusion 30% of ROI of CI patients have lesions and the positive rate of the left side was higher. The biological rCBF values of all lobes were different. Therefore, rCBF index could be used to reflect whether the ROI is normal. rCBF≥0.7 could be used as a sign to quantitatively assess abnormal ROI in clinical practice.