1.DETECTION OF PLATELET MEMBRANE GLYCOPROTEIN WITH PAP-IMMUNOHISTOCHEMICAL TECHNIQUE
Xiaosheng GU ; Yiqi XUE ; Jiazhen ZHU ;
Chinese Journal of Forensic Medicine 1987;0(03):-
The platelets in the human mixed thrombus,hyaline thrombus antemortemand postmrtem skin incision wounds were detected by the PAP-immunohisto-chemical technique using monoclonal antibodies against the human platelet mem-brane glycoprotein(GPIb,GPIIIa).The positive reaction were observed inthe mixed thrombus,hyaline thrombus and in all the antrmortem skin incisionwounds,but not in the postmortem skin wounds.The significance of theapplication of this technique in the forensic medicine practice was discussed.
2.Subjective well-being simplify scale in college students:development, reliability and validity
Shijun GU ; Xianghua ZHU ; Xiumei XUE ; Guangli LIANG ; Juan QIAO
Chinese Journal of Behavioral Medicine and Brain Science 2013;22(8):761-763
Objective To develop subjective well-being simplify scale (SWBSS),and examine its reliability and validity.Methods A self-report SWBSS containing 13 items was developed on college student and research literature.2374 college students completed SWBSS,Index of Well-Being(IWB),World Health of Organization Quality of Life Brief Scale (WHOQOL-BREF),Beck Depression Rating Scale (BDI) and Self-rating Anxiety Scale(SAS).Results ①Exploratory factor analysis results showed that SWBSS had 1 factor,and accounted for 41.768% of variance.②Confirmatory factor analysis results indicated that x2/df =2.208,RMR =0.016,RMSEA =0.032 ; GFI =0.986,AGFI =0.974,NFI =0.978,RFI =0.966,IFI =0.988,TLI =0.981,CFI =0.988 ; PGFI =0.552,PNFI =0.639,PCFI =0.646 ; construct reliability =0.901.③The Cronbach'α coefficient,split-half reliability,stability coefficient of SWBSS was 0.876,0.817 and 0.740 (P < 0.01).The SWBSS scores was significantly correlated with the scores of IWB,WHOQOL-BREF,BDI and SAS (r =0.706 ~ 0.892,r =-0.650 ~-0.580,P<0.01).Conclusion The stability,internal consistency,and validity of the SWBSS are good and meet with psychometric standard.
3.Characteristics and risk factors for healthcare-associated infection in pa-tients with severe chronic hepatitis B
Xue GU ; Xueming JING ; Ling LI ; Qirong ZHU ; Xiaoping MEI
Chinese Journal of Infection Control 2016;15(7):518-520,523
Objective To investigate the clinical characteristics and risk factors for healthcare-associated infection (HAI)in patients with severe chronic hepatitis B (CHB),and provide theoretical basis for preventing and controlling HAI.Methods Retrospective survey was used to investigate the occurrence of HAI in hospitalized patients with severe CHB in a hospital between January 2012 and January 2015,risk factors for HAI were analyzed. Results A total of 126 patients with severe CHB were investigated,49 patients developed 106 times of HAI, incidence of HAI was 38.89%.The main HAI site was respiratory tract (n=47,44.34%),the next was abdominal cavity (n=34,32.08%).A total of 76 isolates of pathogens were detected,gram-negative bacteria,gram-positive bacteria,and fungi accounted for 53.95%(n =41 ),43.42%(n =33),and 2.63%(n =2)respectively.Risk factors for HAI in patients with severe CHB were patients ’ age ≥ 60 years, length of hospital stay ≥ 30 days, complications,invasive operation,serum albumin < 35 g/L,and white blood cell count (WBC)< 4 × 109/L. Conclusion Incidence of HAI in patients with severe CHB is high,the majority are respiratory tract and abdominal cavity infection,risk factors are old age,long length of hospital stay,invasive operation,hypoalbuminemia,and low WBC count.
4.Antisense oligonucleotides targeting seed sequence of miR-155 and its ap-plications in multiple myeloma
Maoxiao FENG ; Rongxuan ZHU ; Xiaochuang LUO ; Chunming GU ; Xue ZENG ; Jia FEI
Chinese Journal of Pathophysiology 2014;(8):1368-1373
[ABSTRACT]AIM:ToinvestigatetheroleoftinyantisensenucleicacidagainstmiR-155(tinyantimiR-155, t-antimiR-155) in multiple myeloma cells .METHODS:According to the seed sequence of miR-155, t-antimiR-155 was designed and synthesized .t-antimiR-155 was transfected by Lipofectamine TM 2000 into RPMI-8266 cells.The cells were di-vided into t-antimiR-155 group, scrambled control (SCR) group and blank control group .The growth-inhibitory potencies were measured by MTT assay .The ability of cell colony formation was detected by cell colony formation assay .The cell ap-optosis was assessed by flow cytometry with annexin V /PI double staining .RESULTS: The best concentration and time were 0.4 μmol/L and 48 h, respectively.The cell colony forming experiment showed that the circumstances of forming cell community in t-antimiR-155 group was weaker than that in SCR group , and the colony formation inhibitory rate of former was significant higher than the latter .Compared with SCR group , the cell apoptosis in t-antimiR-155 group significantly in-creased.CONCLUSION: The t-antimiR-155 inhibits the progression of multiple myeloma cells by interfering with miR-155.miR-155 may serve as a potential target in gene therapy for treating multiple myeloma .
5.Severe acute respiratory syndrome.
Mao-de LAI ; Yi-min ZHU ; Xue-mei GU
Journal of Zhejiang University. Medical sciences 2003;32(3):167-170
Severe acute respiratory syndrome(SARS), caused by SARS- associated coronavirus(SCV), is the first severe infectious disease in this century. SARS is pathologically characterized by interstitial exudative inflammation of lung with the formation of hyaline membrane in acute phase. Haemorrhagic inflammation exists in extrapulmonary organs. Clinical diagnosis is a dynamic process and includes the suspected case, probable case and definite case. Diagnostic standard of SARS will be revised with further understanding of the disease. Chinese term of SARS has been recommended in the paper.
Humans
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Severe Acute Respiratory Syndrome
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diagnosis
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epidemiology
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pathology
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Terminology as Topic
6.New beta-carboline alkaloids contained in Psammosilene tunicoides.
Wei WANG ; Lin YUAN ; Xue-Zhu GU ; Wen-Yi KAN
China Journal of Chinese Materia Medica 2012;37(21):3240-3242
OBJECTIVETo study the chemical constituents of Psammosilene tunicoides.
METHODThe two chemical constituents were separated by various chromatographic methods, and their structures were identified on the basis of spectroscopic analysis.
RESULTTwo beta-carboline alkaloids were separated from normal butanol fraction of P. tunicoides, and identified as 1-acetyl-3-methoxycarbonyl-beta-carboline (1) and 1-acetyl-3-methoxycarbonyl-4-hydroxyl-beta-carboline (2).
CONCLUSIONCompound 1 is separated from this plant as natural products for the first time, and compound 2 was a new compound.
Alkaloids ; analysis ; Carbolines ; analysis ; Caryophyllaceae ; chemistry
7.Incidence of acute kidney injury according to AKI Network after cardiac surgery and analysis of risk factors and outcome
Miaolin CHE ; Yi LI ; Xinyue LIANG ; Huili DAI ; Mingli ZHU ; Leyi GU ; Jiaqi QIAN ; Zhaohui NI ; Song XUE ; Yucheng YAN
Chinese Journal of Nephrology 2009;25(4):265-271
Objective To investigate the incidence, risk factors and outcome of acute kidney injury (AKI) following cardiac surgeries. Methods Clinical data of 1056 patients undergoing open heart surgery in Renji Hospital from January 2004 to June 2007 were retrospectively analyzed. Univariate and multivariate analyses were used to evaluate possible pre-,intra-, and post-operative parameters associated with AKI according to AKI Network (AKIN). Results Of the 1056 patients, 328 (31.06%) developed AKI. In-hospital mortality was 4.07% in all discharges while 11.59% in AKI patients (P<0.01). Multivariate logistic regression analysis revealed that increased age (OR=1.40), pre-operative hyperurieemia (OR=1.97), pre-operative left ventricular insufficiency (OR=2.53), combined surgery (OR=2.79), prolonged operation time (OR=1.43), post-operative circulation volume insufficiency (OR=11.08) were risk factors of AKI. Conclusions AKI is a common complication and associated with increased mortality following cardiac surgery. Increased age, pre-operative hyperuricemia, pre-operative left ventricular insufficiency, combined surgery, prolonged operation time, post-operative circulation volume insufficiency are useful in stratifying risk factors for the development of AKI.
8.Application of near infrared spectroscopy combined with particle swarm optimization based least square support vactor machine to rapid quantitative analysis of Corni Fructus.
Xue-song LIU ; Fen-fang SUN ; Ye JIN ; Yong-jiang WU ; Zhi-xin GU ; Li ZHU ; Dong-lan YAN
Acta Pharmaceutica Sinica 2015;50(12):1645-1651
A novel method was developed for the rapid determination of multi-indicators in corni fructus by means of near infrared (NIR) spectroscopy. Particle swarm optimization (PSO) based least squares support vector machine was investigated to increase the levels of quality control. The calibration models of moisture, extractum, morroniside and loganin were established using the PSO-LS-SVM algorithm. The performance of PSO-LS-SVM models was compared with partial least squares regression (PLSR) and back propagation artificial neural network (BP-ANN). The calibration and validation results of PSO-LS-SVM were superior to both PLS and BP-ANN. For PSO-LS-SVM models, the correlation coefficients (r) of calibrations were all above 0.942. The optimal prediction results were also achieved by PSO-LS-SVM models with the RMSEP (root mean square error of prediction) and RSEP (relative standard errors of prediction) less than 1.176 and 15.5% respectively. The results suggest that PSO-LS-SVM algorithm has a good model performance and high prediction accuracy. NIR has a potential value for rapid determination of multi-indicators in Corni Fructus.
Algorithms
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Calibration
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Cornus
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chemistry
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Drugs, Chinese Herbal
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chemistry
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Fruit
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chemistry
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Least-Squares Analysis
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Models, Theoretical
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Neural Networks (Computer)
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Quality Control
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Spectroscopy, Near-Infrared
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Support Vector Machine
9.Research progress on processed Chinese medicine in vivo.
Jian-hong CHEN ; Hui LIU ; Lan YAO ; Ying CHEN ; Yin-lian MA ; Xue-zhu GU ; Cun ZHANG
China Journal of Chinese Materia Medica 2014;39(21):4149-4152
Processed Chinese medicine is the core of traditional Chinese medicine (TCM) industry chain,which directly affects the clinical efficacy and. safety of Chinese patent medicine and clinical formula Decoction pieces. Studied the variation of effective substance in vivo Chinese medicine processing before and after processed, clarifying the effective substance and processing principle is a top priority of the development of Chinese medicine processing. The traditional research method chiefly focus on the variation about chemicals in vitro of processed Chinese medicine, it cannot reveal that the integrity and complexity of processed Chinese medicine efficacy changes, so the change process is the focus of future research in vivo on the base of effective substance of TCM This paper described the research on the base of effective substance of TCM and Processed Chinese medicine research status in vitro, discussed the analytical methods (plasma chemistry, pharmacokinetics, metabonomics) of the dynamic process in vivo about processed Chinese medicine, and pointed out development and related problems in process in vivo on the base of effective substance of TCM, which could provided research ideas and methods for in-depth interpretation of Chinese medicine processing mechanism.
Animals
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Drugs, Chinese Herbal
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analysis
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pharmacokinetics
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Humans
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Medicine, Chinese Traditional
10.Analysis of volatile ingredients in Gardeniae Fructus and its processed products by GC-MS.
Hui LIU ; Lan YAO ; Jian-hong CHEN ; Xue-zhu GU ; Yin-lian MA ; Ying CHEN ; Pu-ling LI ; Cun ZHANG
China Journal of Chinese Materia Medica 2015;40(9):1732-1737
Gardeniae Fructus contains volatile ingredients, however, the species and proportions in different processed products of Gardeniae Fructus are different. In this experiment, volatile ingredients were separated by steam distillation with content of 1.2, 1.0, 0.9, 0.7 µL · g(-1) in Gardeniae Fructus, fried Gardeniae Fructus, stir-baked Gardeniae Fructus, Gardeniae Fructus fried into carbon respectively. One hundred and twenty-four kinds of volatile components were identified by GC-MS. Fifty-three kinds of volatile ingredients consisted in Gardeniae Fructus accounting for 93.85%, 54 kinds in fried Cardeniae Fructus accounting for 92.01%, 32 kinds in stir-baked Cardeniae Fructus accounting for 91.59% and 43 kinds in Gardeniae Fructus fried into carbon accounting for 90.81%. In this paper, analysis of Gardeniae Fructus by GC-MS provides a scientific basis for elucidating the mechanism of different processed products.
Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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Gardenia
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chemistry
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Gas Chromatography-Mass Spectrometry
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Molecular Structure
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Volatile Organic Compounds
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chemistry