1.STUDIES ON DIAGNOSIS OF FALCIPARUM MALARIA BASED ON AMPLIFYING SPECIFIC SSUrDNA FRAGMENT WITH NESTED PCR
Lei WAN ; Peixia CHEN ; Caifang XUE ; Shaochun JIANG
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(03):-
Two pairs of primers specific to small subunit ribosomal DNA of Plasmodium falciparum were designed and the expected SSUrDNA fragment was amplified for detecting P.falciparum infection with double-ternperature-nested polymerase chain reaction using DNA prepared by boiling method. The results showed that the nested PCR could amplify a constant size of desired SSUrDNA fragment of P. falciparum which was further confirmed by digestion of restrlction endonuclease and could detect parasitemia level of 0. 8 ? 10-6. It has great potentials for identifying Plasmodium species in ring form of erythrocytic stage and detecting mixed Plasmodium infections. Therefore, it is suggested that this method is sensitive, accurate, simple and rapid in detecting Plasmodium falciparum in blood samples for malaria diagnosis.
2.Reliability and validity of the tree-drawing test in medical undergraduates
Chao WAN ; Xue RAN ; Wen SHI ; Quan CHEN ; Wei LIU
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(10):946-948
Objective To assess the reliability and validity of the Tree-Drawing Test in medical college students.Methods The study randomly selected 312 aged 19 to 23-year-old medical students to take part in TreeDrawing Test.In addition,a total of 275 college students were selected to receive re-test,30 days late and Pearson correlation coefficient of two tests were calculated.The three raters were invited to assess 30 trees painting score,analyzing the Kendall coefficient of concordance between the scores to verify raters' reliability; parts of students also participated in the 16PF test,SAS,SDS test,analyzing the correlation coefficient between the various test results,in order to assess the effectiveness of the Tree-Drawing Test.Results The re-test reliability in different time was 0.570-0.733 and 0.341-0.713 (P<0.05),the raters' reliability was 0.491 ~ 0.626(P<0.05),there are some correlations between Tree-Drawing Test and 16PF,SAS,SDS.Conclusion The Tree-Drawing Test has good reliability and validity; it can be applied to the detection of college students' psychological assessment and psychological problems.
3.Effect of butylphthalide on the expression of GFAP and VEGF in the hippocampus of rats with (Alzheimer's) disease
Deren HOU ; Li XUE ; Kun CHEN ; Yi TIAN ; Shun WAN
Journal of Central South University(Medical Sciences) 2010;35(2):111-115
Objective To determine the expression of glial fibrillary acidic protein(GFAP) and vascular endothelial growth factor(VEGF) in the hippocampus of rats with Alzheimer's disease(AD), and to determine the effect of butylphthalide on them and its significance. Methods Sixty male adult rats were randomly divided into a model group, a Butylphthalide group, and a control group. AD models were established by injecting β-amyloid protein 1-42 into the hippocampus of rats. Sixty days later,the rats were sacrificed and both sides of the hippocampus were sectioned for immunohistochemistry. Results Positive cells of GFAP in the hippocampus of the model group increased and the expression of VEGF decreased statistically, compared with the control group(P<0.01). The positive cells of GFAP in the hippocampus of the butylphthalide group decreased and the expression of VEGF increased significantly, compared with the model group(P<0.05). Conclusion Butylphthalide may protect the neuron-vascular unit of the hippocampus of Alzheimer model rats by inhibiting the expression of GFAP and increasing the expression of VEGF.
4.Interstitial granuloma fungoides: a case report
Hao CHEN ; Yanning XUE ; Chuan WAN ; Xuesi ZENG ; Jianfang SUN
Chinese Journal of Dermatology 2011;44(12):851-853
A 36-year-old woman presented with a 3-year history of pruritic erythema and scaling on the trunk and extremities.Dermatological examination revealed ill-defined light pink macules with white lamellar scales on the chest,abdomen and buttocks.Histologically,there was a focal mononuclear cell infiltrate in the superficial dermis,with the epidermotropism of some cells and mild atypia of epidermotropic cells,as well as an interstitial mononuclear cell infiltrate and mild deposition of mucin between the collagen fibers in the middle dermis.CD3 and CD4 were expressed by scattered mononuclear cells infiltrating the upper and middle dermis.Based on these data,the patient was diagnosed with interstitial granuloma fungoides.
5.Relationship between the expression of P-glycoprotein,glutathione S-transferase-? and thymidylate synthase proteins and adenosine triphosphate tumor chemosensitivity assay in cervical cancer
Guo-Lan GAO ; Hong-Ying WAN ; Xue-Sen ZOU ; Wen-Xue CHEN ; Yue-Qing CHEN ; Xiu-Zhen HUANG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(03):-
0.05).Conclusions ATP-TCA could be used to individualize chemotherapy by selecting agents for particular patients of cervical cancer.The expression of GST-? and TS protein might be useful biomarkers to predict the resistance to DDP and 5-FU in patients with cervical cancer.
6.Distribution of HBV genotypes and clinical characteristics of HBV-related hepatocellular carcinoma patients in Deyang District, Sichuan Province.
Jia-hong YANG ; Gao CHEN ; Hao ZHANG ; Xue-bing CHEN ; Xiu WANG ; Wan-rong LUO
Chinese Journal of Hepatology 2013;21(6):473-474
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Carcinoma, Hepatocellular
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diagnosis
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epidemiology
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virology
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Child
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China
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epidemiology
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Female
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Genes, Viral
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Genotype
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Hepatitis B virus
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genetics
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Humans
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Liver Neoplasms
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diagnosis
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epidemiology
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virology
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Male
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Middle Aged
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Viral Load
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Young Adult
7.Spindle cell variants of diffuse large B cell lymphoma: report of 2 cases.
Jun-chen WANG ; Da-ren SHI ; Xue-lian FU ; Chang-li LU ; Wan-ping LU
Chinese Journal of Pathology 2005;34(1):55-56
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Breast
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pathology
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Breast Neoplasms
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drug therapy
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pathology
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Cyclophosphamide
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therapeutic use
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Diagnosis, Differential
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Doxorubicin
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therapeutic use
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Female
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Follow-Up Studies
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Humans
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Lymphoma, B-Cell
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drug therapy
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pathology
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Lymphoma, Large B-Cell, Diffuse
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drug therapy
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pathology
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Male
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Middle Aged
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Prednisone
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therapeutic use
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Sarcoma
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pathology
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Spleen
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pathology
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Splenic Neoplasms
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drug therapy
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pathology
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Vincristine
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therapeutic use
8.Comparison of genotype distribution of patients with acute hepatitis B infection or chronic hepatitis B infection in Shanghai
Xuesong LIANG ; Mobin WAN ; Chengzhong LI ; Hao XU ; Jianya XUE ; Ruiying ZHENG ; Jixiu CHEN
Chinese Journal of Infectious Diseases 2009;27(1):23-26
Objective To investigate the distribution of genotypes in chronic HBV infection (CHB) and acute HBV infection (AHB) patients in Shanghai. Methods Sixty-two patients with AHB and 73 patients with CHB admitted to ('hanghai Hospital of Shanghai between 2003 and 2007 were studied. Viral genotypes of all the patients were determined by direct gene sequencing.Meanwhile, epidemiological, clinical and biochemical parameters of all patients were collected. Mean values of different groups were compared by t test while frequency was compared by chi square test. Results The major prevalent genotypes in both AHB and CHB patients were genotype B and C (48.4% vs 51.6% in AHB patients and 26.0% vs 74.0% in CHB patients). The proportion of genotype B was higher in AHB patients compared to CHB patients (P= 0.02). Epidemiological factors and clinical outcomes were not statistically different among patients with different viral genotypes. The proportion of genotype C was much higher in CHB patients compared to AHB patients (P=0.006). The main transmission route of AHB was heterosexual interaction which was 18 out of 62 (29.0%), but in CHB patients, it was prenatal transmission which was 38 out of 73 (52.1%). Conclusions In shanghai, the main HBV genotypes in both AHB and CHB patients are genotype B and C. The proportion of genotype B is relatively high in AHB patients while proportion of genotype C is more common in CHB patients. There is no significant relationship between genotypes and the clinical outcomes of AI-IB patients.
9.Dissolution testing combined with computer simulation technology to evaluate the bioequivalence of domestic amoxicillin capsule.
Rui-Xue PAN ; Yuan GAO ; Wan-Li CHEN ; Yu-Lan LI ; Chang-Qin HU
Acta Pharmaceutica Sinica 2014;49(8):1155-1161
Re-evaluation of bioequivalence of generic drugs is one of the key research focus currently. As a means to ensure consistency of the therapeutic effectiveness of drug products, clinical bioequivalence has been widely accepted as a gold standard test. In vitro dissolution testing based on the theory of the BCS is the best alternative to in vivo bioequivalence study. In this article, the conventional dissolution method and flow-through cell method were used to investigate the dissolution profiles of domestic amoxicillin capsules in different dissolution media, and the absorption behavior of the drugs with different release rates (t85% = 15-180 min) in the gastrointestinal tract was predicted by Gastro Plus. The flow-through cell method was thought better to reflect the release characteristics in vivo, and amoxicillin capsules with regard to the release rates up to 45 min (t85% = 45 min) were having a satisfied bioequivalence with the oral solution according to the C(max) and AUC. Although two different dissolution profiles of domestic amoxicillin capsules were found by flow-through cell methods, prediction results revealed that domestic capsules were probably bioequivalent to each other.
Amoxicillin
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pharmacokinetics
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Capsules
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Computer Simulation
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Gastrointestinal Tract
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Humans
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Software
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Solubility
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Therapeutic Equivalency
10.Construction of double-copy and x gene deleted hepatitis B virus DNA expression plasmid and cells transfection study
Yu SONG ; Mobin WAN ; Wenjie LI ; Jian CHEN ; Chaoping FANG ; Huibin XUE ;
Academic Journal of Second Military Medical University 2001;0(09):-
Objective:To construct double copy and x gene deleted HBV expression plasmid and study its expression in Hep3B cell line. Methods:The double copy HBV DNA ( adr Ⅰ) was used to inactivate HBV x gene by inserting mutation and gene recombination. The inserted 55 bp DNA sequence was synthesized artificially; the insertion point was ApaL Ⅰ of x gene area. After recombination, an x gene defected HBV plasmid containing single P, S and C gene was constructed,which can express in mammalian cell line. Another plasmid carrying double copy HBV DNA with normal x gene was constructed as contrast. Both were used to transfect Hep3B cells. Then the cells were screened by G418 and HBV virus in culture medium were isolated and detected by fluorescence quantitative PCR. Results: Plasmids pcDNA3 KN F1F2 and pcDNA3 ES HBV2 were constructed successfully. After cell transfection, the HBV DNA was highly expressed with both plasmids on the 3 rd, 6 th,14th day. Conclusion: The plasmids constructed can express in Hep3B cell line and cause HBV replication; x gene defected HBV gene has no effect on HBV replication in Hep3B cell line.