1.Study on the correlation between different treatment methods and postoperative outcome in elderly patients
Xue YANG ; Tienan FENG ; Ping LI
Chongqing Medicine 2017;46(14):1953-1955
Objective To analyze the correlation between the diagnosis and treatment and the postoperative death and complication of the elderly patients.Methods A total of 1 932 cases of surgical patients (over 70 years old) were collected from a three-level general hospital 2013-2015.Their medical information of the treatment methods were recorded,including incision category,surgical approach,surgical grade and patient outcome after surgery(death or postoperative complications).Then we analyzed the incidence of death or postoperative complications,as well as the effects of treatment and postoperative outcome on complications or mortality.Results The admission status and incision type were the risk factors of postoperative death in elderly patients.Admission status pollution type,surgical approach and surgical grade were the risk factors of postoperative complications.Conclusion In order to reduce the risk of postoperative death and complications in elderly patients,the effect of admission and incision on the postoperative risk of elderly patients should be evaluated.
2.Construction and application of clinical pathway quality evaluation system
Xue YANG ; Ping LI ; Shensheng TAN
Chinese Journal of Hospital Administration 2017;33(7):509-512
Objective To establish a comprehensive evaluation system for clinical pathway quality for evaluation and verification.Methods Literature retrieval, clustering mergers and experts consultation method were used to determine indicators for clinical pathway quality evaluation, while gray correlation method was employed to calculate indicators weight coefficient and to evaluate indicators scoring,and sum indicators weighted scoring, so as to establish a clinical pathway quality comprehensive evaluation model.Indicator data of Shanghai′s 18 tertiary hospitals were used for verification.Results A total of 12 evaluation indicators, such as entry rate, mutation rate, completion rate and average length of stay were selected.After the weight calculation and grade scoring, the mathematical model of clinical pathway quality comprehensive evaluation was constructed.Data of these hospitals were put into the evaluation system for assigning ratings calculation, harvesting the overall score and ranking of each hospital.These data can help find out key management and key hospital intervention indicators in the next stage.Conclusions Different hospitals will be put on the same platform for comparison in this clinical pathway evaluation system, and the results can be quantified.The authors suggest that the next stage of health administration focus on the management of the hospitals and the key management of the sectors, making possible the prospective management of clinical pathways.
3.Preliminary study on three kinds of method of interleukin 1 (IL-1) assay
Xue-Ping WANG ; Ming-Jiu YANG ;
Chinese Journal of Immunology 1985;0(01):-
In present paper,we studied three kinds of method of assay for IL-1:thymocyte proliferation assay;L_(?) cell proliferation assay and pyrogen assay.Stimultaneously we used ~(125)I-UdR in stead of ~3H-TdR.The results show that all of three kinds of method of assay for IL-1 can measure IL-1 activity of supernatants from cultured rat alveolar M? stimulated by LPS in vitro.It suggests that ~(125)I-UdR can incorporate into DNA synthesis of cell and mouse fibroblast cell line L_(?) can response to IL-1 of supernatant from cultured rat alveolar M? stimulated by LPS.
4.Application of transitional problem-based learning in teaching of Department of Gynecology and Obstetrics
ping, LI ; yue-zhen, XUE ; yang, ZOU ; qing, MIAO
Journal of Shanghai Jiaotong University(Medical Science) 2008;0(S1):-
0.05).But transitional PBL had much priority in enhancing students' learning interests. Conclusion The applicational of transitional PBL requires to be further deepened and improved in the realm of medical education.
5.Improving doctor-patient relationship, enhancing the oral cavity medical service effect
Ming-cong YANG ; Xiao-ping FAN ; Xue-rong XIANG
Chinese Journal of Medical Education Research 2011;10(7):851-853
While the medicine pattern of biomedicine turn to biological-psychology-society, the medical trouble communication becomes more and more important in the medical service. Good medical trouble communication ability is the essential condition of doctor. As oral cavity clinicians, only by gasping the principle of communication can we appropriately utilize some skills of communication exchange,establish the good medical trouble relations with the patient and achieve the good treatment result finally.
6.Study on experimental teaching reform of the clinical hematology and hematologic examination
Yiqing YANG ; Subing XUE ; Yanchao ZHANG ; Ping LI ; Wenzhi ZHENG
Chinese Journal of Medical Education Research 2003;0(03):-
Clinical hematology and hematologic examination is a strong practical curriculum, and experimental class is very important in the aspects of teaching.In order to improve the quality of teaching and train compound laboratory talents with high quality,several effective reform mea-sures were carried out based on the years of teaching practice by the department:improving the methods of experimental teaching;using modern means of teaching;emphasizing on the analysis of experimental results,formulating the rigorous experimental evaluation system and starting the second class etc.
7.Construction of human embryonic kidney cells exhibiting human preproenkephalin gene expression
Feng BAI ; Baozhong YANG ; Zhaoxia XUE ; Yufei PAN ; Ping HUANG
Chinese Journal of Anesthesiology 2012;32(6):673-675
ObjectiveTo construct human embryonic kidney cells (HEK293) modified with human preproenkephalin (hPPE) gene.MethodshPPE gene fragments were obtained from recombinant plasmid pcDNA3.1( + )/hPPE by using restriction endonuelease Hind Ⅲ and Not Ⅰ.Homologous recombination of lentivirus and hPPE gene was produced by using recombinant DNA technology.HEK293 cells were then transfected with the recombinant lentivirus vectors.The expression of hPPE gene in HEK293 cells was detected by Western blot.ResultsThe results of DNA sequencing indicated that the positive clone of recombinant lentivirus was completely consistent with sequencing of hPPE in Genebank.The titer of the concentrated virus was 2.07 × 108 TU/ml.GFP fluorescence was not seen in HEK293 cells transfected with the lentiviral vector under fluorescence microscope.A strong fluorescence was seen in HEK293 cells transfected with Ubc-GFP-L.V.empty viral vector.Positive expression of hPPE was demonstrated in HEK293 cells transfected with lentiviral vector by Western blot.Conclusion HEK293 cells modified with hPPE gene were successfully constructed and the target gene hPPE was stably expressed in HEK293 cells.
10.pRNA: A Safe,Stable and Large Capacity Molecular Vehicle for Gene Therapy
Feng-Ping XU ; Jun YANG ; Xue-Song FENG ; Li-Jia AN ;
China Biotechnology 2006;0(06):-
The molecular delivery vectors used in gene therapy need provide the features of safety,stability, efficiency and capacity. The current studies on the structure and action mechanism of pRNA, a packaging RNA of phage?29, showed that pRNA with multiple binding sites can through cell membrane easily and escort exogenous molecules to target cell, without inducing immune reaction. As an ideal nano-scale gene therapy vehicle, pRNA presents a promising application in delivering multiple therapeutic components to detect and treat human diseases.