1.Transcriptome-based bioinformatics analysis of Arnebia euchroma ERF transcription factor family.
Teng XIE ; Sheng WANG ; Lei HUANG ; Xue WANG ; L-ping KANG ; Lan-ping GUO
China Journal of Chinese Materia Medica 2014;39(24):4732-4739
Twenty-seven ERF transcription factor family genes were isolated from Arnebia euchroma, with an average size of 1,010 bp, each gene encoded a 212 amino acids on average. The gene structure and expression of physicochemical properties, subcellular localization, signal peptides, senior structural domains and conservative forecasting, and analysis of A. euchroma were studied comparing with ERF gene gi261363612 of Lithospermum erythrorhizon, and phylogenetic analysis of A. euchroma ERF family was carried out. The results showed the existence of three conserved domains in this family, the senior structure based on random coil and it clustered into CBF/DREB and ERF subfamilies.
Amino Acid Motifs
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Amino Acid Sequence
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Boraginaceae
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genetics
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Cloning, Molecular
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Computational Biology
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Genome, Plant
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genetics
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High-Throughput Nucleotide Sequencing
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Medicine, Chinese Traditional
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Molecular Sequence Data
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Multigene Family
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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Plants, Medicinal
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Protein Structure, Secondary
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Protein Structure, Tertiary
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Sequence Analysis, DNA
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Transcription Factors
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chemistry
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genetics
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Transcriptome
2.Left cervical mass.
Jian-lan XIE ; Xiao-ge ZHOU ; Yan JIN ; Xiao-dan ZHENG ; Xue-jing WEI
Chinese Journal of Pathology 2012;41(3):195-196
Adult
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Diagnosis, Differential
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Female
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Granulomatous Disease, Chronic
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metabolism
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pathology
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Hodgkin Disease
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metabolism
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pathology
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Humans
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Ki-1 Antigen
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metabolism
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Lewis X Antigen
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metabolism
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Lymphoma, Large B-Cell, Diffuse
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metabolism
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pathology
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Lymphoma, Large-Cell, Anaplastic
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metabolism
;
pathology
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Young Adult
3.Facial skin nodules.
Jian-lan XIE ; Xiao-ge ZHOU ; Yan JIN ; Xiao-dan ZHENG ; Xue-jing WEI
Chinese Journal of Pathology 2010;39(6):410-411
Adult
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Antigens, CD20
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metabolism
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CD3 Complex
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metabolism
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Diagnosis, Differential
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Facial Dermatoses
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metabolism
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pathology
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surgery
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Follow-Up Studies
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Humans
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Lymphoma, B-Cell, Marginal Zone
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metabolism
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pathology
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Lymphoma, Large-Cell, Anaplastic
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metabolism
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pathology
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Male
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Pseudolymphoma
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metabolism
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pathology
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surgery
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Skin Neoplasms
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metabolism
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pathology
4.Spindle cell variant of anaplastic large cell lymphoma.
Jian-lan XIE ; Xiao-ge ZHOU ; Yan JIN ; Xiao-dan ZHENG ; Xue-jing WEI
Chinese Journal of Pathology 2010;39(5):340-342
Actins
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metabolism
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Adult
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Diagnosis, Differential
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Granzymes
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metabolism
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Histiocytic Sarcoma
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metabolism
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pathology
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Humans
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Ki-1 Antigen
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metabolism
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Lymph Nodes
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metabolism
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pathology
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Lymphoma, Large-Cell, Anaplastic
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metabolism
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pathology
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Male
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Neoplasms, Muscle Tissue
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metabolism
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pathology
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Protein-Tyrosine Kinases
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metabolism
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Receptor Protein-Tyrosine Kinases
5.Compound danshen injection regulated the expression of AQP3 in the human amnion epithelium cells through JNK signal pathway.
Jing-Jing WANG ; Ying HUA ; Qing-Feng ZHOU ; Ai-Lan XIE ; Xue-Qiong ZHU
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(8):931-935
OBJECTIVETo explore the role of Compound Danshen Injection (CDI) in regulating the expression of aquaporin 3 (AQP3) in human amnion epithelium cells (hAECs), and to study the relation between c-Jun N-terminal kinase (JNK) signal pathway and AQP3.
METHODShAECs were isolated and primarily cultured from term pregnancy with normal amniotic fluid volume and from term pregnancy with oligohydramnios, and then hAECs were further divided into four groups, i.e., the blank control group (A), the SP600125 group (B), the CDI group (C), and the SP600125 +CDI group (D). The cell viability was measured by cell counting kit-8 assay (CCK-8). The expression of total JNK, phosphorylated JNK, and AQP3 were determined by Western blot.
RESULTS(1) In hAECs with normal AFV or with oligohydramnios: There was no statistical difference in the cell viability or the expression of total JNK among the 4 groups (P > 0.05). But there was statistical difference in the expression of p-JNK (P < 0.05). Compared with A group, the expression of p-JNK was obviously down-regulated in B group, but obviously up-regulated in C group (P < 0.05). The expression of p-JNK was significantly lower in D group than in C group, but higher than that in A group or B group (P < 0.05).The AQP3 expression in the hAECs with normal amniotic fluid volume of C group and D group were higher than that in the A group (P < 0.05). However, there was no statistical difference in the AQP3 expression between C group and D group (P > 0.05). In hAECs with oligohydramnios, the expression of AQP3 obviously decreased in B group, but up-regulated in C group (both P < 0.05). The expression of AQP3 was lower in D group than in C group, but higher than in B group (P < 0.05).
CONCLUSIONCDI could regulate the AQP3 expression in hAECs with oligohydramnios via activating the JNK signal pathway.
Amnion ; cytology ; drug effects ; Aquaporin 3 ; metabolism ; Cells, Cultured ; Drugs, Chinese Herbal ; pharmacology ; Epithelial Cells ; drug effects ; metabolism ; Female ; Humans ; JNK Mitogen-Activated Protein Kinases ; metabolism ; MAP Kinase Signaling System ; physiology
6.Relationship between TGF-β1 Gene Polymorphism and Liver Cirrhosis after Hepatitis B Virus Infection:A Meta-Analysis in Chinese Population
Minmin XUE ; Zhongliang XU ; Gongming DONG ; Fang XIE ; Peiqun WU ; Lan BAI
Tianjin Medical Journal 2014;(5):502-506
Objective To evaluate the relationship between genetic polymorphism of transforming growth factor (TGF)-β1 and susceptibility of liver cirrhosis after hepatitis B virus infection in Chinese population. Methods CBM, VIP, CNKI, Wanfang technological periodical full-text databases and Pubmed from set up to July, 2013 were electronically searched to identify case-control studies on the relationship between genetic polymorphism of TGF-β1 promoter 509 site, co-don 869 site and liver cirrhosis after hepatitis B virus infection. The data were quantitatively analyzed by RevMan 5.1 soft-ware after assessing the quality of included studies. Results Six case-control studies were selected for Meta-analysis based on our inclusion and exclusion standards. The results of Meta-analysis showed that the pooled OR value for liver cir-rhosis among Chinese patients after hepatitis B virus infection with T allele of TGF-β1 gene at promoter 509 was 1.02 (95%CI:0.67-1.54), the pooled OR values for patients with TT and CT genotypes were 0.80 (95%CI:0.36-1.78). OR values for pa-tients with C allele of TGF-β1 gene at codon 869 was 1.05 (95%CI:0.69-1.62), the pooled OR values for patients with CC and CT genotypes were 0.98 (95%CI:0.48-2.00). No significant publication bias was found. Conclusion The genetic poly-morphism of TGF-β1 at promoter 509 and codon 869 showed no association with susceptibility of liver cirrhosis after hepati-tis B virus infection in Chinese population.
7.Clinical effect analysis in the treatment of acute limb arterial critical ischemia
Guanhua XUE ; Changning HAO ; Lei LYU ; Hui XIE ; Xiangjiang GUO ; Xiaozhong HUANG ; Meng YE ; Lan ZHANG
International Journal of Surgery 2016;43(12):814-817
Objective To discuss the therapeutic effect in the treatment of the acute limb arterial critical ischemia.Methods Collect thirty-nine cases of acute limb arterial critical ischemia in Renji Hospital from Janary 2014 to July 2016.According to the patients' manifestation,these operations were porfermed including thrombectomy,cathetery-directed thrombolysis,mechanical suction bolt,percutaneous angioplasty and stenting.The effect and complications were observed.Results The eighteen patients in 39 cases (46.2%) were dead,including 5 cases without operation,13 operation.The eight cases were amputated during 34 cases of operations.In the 21 out-patients safely,2 cases were not followed up.The time of follow-up was from 3 to 27 months,on average 14.3 months.During the 21 patients,5 cases died from heart cerebrovascular or tumor diseases,3 cases with footdrop,2 cases with toe amputations,3 cases with distal leg and foot anesthesias.Conclusions The patiens with acute limb arteries critical ischemia must be treated as early as,and reinforced the management of multiple organ function,which may improve the diseases' therapeutic effect.
8.Effects of dynamic ventilatory factors on ventilator-induced lung injury in acute respiratory distress syndrome dogs
Rui-Lan WANG ; Kan XU ; Kang-Long YU ; Xue TANG ; Hui XIE
World Journal of Emergency Medicine 2012;3(4):287-293
BACKGROUND: Mechanical ventilation is a double-edged sword to acute respiratory distress syndrome (ARDS) including lung injury, and systemic inflammatory response high tidal volumes are thought to increase mortality. The objective of this study is to evaluate the effects of dynamic ventilatory factors on ventilator induced lung injury in a dog model of ARDS induced by hydrochloric acid instillation under volume controlled ventilation and to investigate the relationship between the dynamic factors and ventilator-induced lung injuries (VILI) and to explore its potential mechanisms. METHODS: Thirty-six healthy dogs were randomly divided into a control group and an experimental group. Subjects in the experimental group were then further divided into four groups by different inspiratory stages of flow. Two mL of alveolar fluid was aspirated for detection of IL-8 and TNF-α. Lung tissue specimens were also extracted for total RNA, IL-8 by western blot and observed under an electronic microscope. RESULTS: IL-8 protein expression was significantly higher in group B than in groups A and D. Although the IL-8 protein expression was decreased in group C compared with group B, the difference was not statistically significant. The TNF-α ray degree of group B was significantly higher than that in the other groups (P<0.01), especially in group C (P>0.05). The alveolar volume of subjects in group B was significantly smaller, and cavity infiltration and cell autolysis were marked with a significant thicker alveolar septa, disorder of interval structures, and blurring of collagenous and elastic fiber structures. A large number of necrotic debris tissue was observed in group B. CONCLUSION: Mechanical ventilation with a large tidal volume, a high inspiratory flow and a high ventilation frequency can cause significant damage to lung tissue structure. It can significantly increase the expression of TNF-α and IL-8 as well as their mRNA expression. Furthermore, the results of our study showed that small tidal ventilation significantly reduces the release of pro-inflammatory media. This finding suggests that greater deterioration in lung injury during ARDS is associated with high inspiratory flow and high ventilation rate.
9.Submandibular lymphadenopathy.
Xue-jing WEI ; Xiao-ge ZHOU ; Ping REN ; Yuan-yuan ZHENG ; Jian-lan XIE ; Xiao-dan ZHENG
Chinese Journal of Pathology 2012;41(5):342-344
Adult
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Antigens, CD20
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metabolism
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Burkitt Lymphoma
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metabolism
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pathology
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Histiocytic Necrotizing Lymphadenitis
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metabolism
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pathology
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Humans
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Lymphatic Diseases
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metabolism
;
pathology
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Lymphoma, Large B-Cell, Diffuse
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metabolism
;
pathology
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Lymphoma, Large-Cell, Anaplastic
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metabolism
;
pathology
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Neprilysin
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metabolism
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Proto-Oncogene Proteins c-bcl-6
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metabolism
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Receptors, Complement 3d
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metabolism
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Submandibular Gland Diseases
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metabolism
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pathology
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Young Adult
10.Hypoxia-inducible factor-1α expression in renal tissue of rats with paraquat poisoning.
Ying XIONG ; Rui-lan WANG ; Hui XIE ; Xue TANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(1):10-13
OBJECTIVETo observe the pathological changes of renal tissue in the rats with paraquat (PQ) poisoning as well as the serum creatinine (SCr) levels and expression levels of hypoxia-inducible factor-1α (HIF-1α) and transforming growth factor-β (TGF-bgr;) in renal tissue at different time points after PQ poisoning, and to investigate the association of HIF-1α with renal injury after PQ poisoning.
METHODSForty-eight healthy male Sprague-Dawley rats were randomly divided into control group (n = 6) and PQ group (n = 42). The control group was given a single dose of 1 ml saline by gavage; the PQ group was given a single dose of 1 ml PQ (50 mg/kg), which was prepared by diluting 20% raw liquid of PQ with saline, by gavage. The PQ group was further divided into 2, 6, 12, 24, 48, 72 and 120 h PQ subgroups (n = 6 for each subgroup) to be examined at 2, 6, 12, 24, 48, 72, or 120 h after gavage. Their arterial blood was collected for blood gas analysis as well as blood lactic acid (BLA) and SCr measurement; renal sections were subjected to HE staining; the protein expression of HIF-1α and TGF-β in renal tissue was measured by Western blot.
RESULTSThe BLA level and SCr level began to rise at 6h after poisoning. Compared with the control group, the 6, 12, 24, 48, 72 and 120 h PQ subgroups had significantly increased BLA and SCr levels (P < 0.05); the 72 and 120 h PQ subgroup showed hypoxemia (P < 0.05). The protein expression of HIF-1α in PQ group increased significantly at 6h and reached the peak level at 72 h, with a significant difference from that in the control group at 6, 12, 24, 48, 72, and 120 h (P < 0.05). The protein expression of TGF-β in PQ group began to rise at 24 h, reached the peak level at 72 h, and declined at 120 h, with a significant difference from that in the control group at 24, 48, and 72 h (P < 0.05). The protein expression of HIF-1α was positively correlated with SCr level (r = 0.9308, P = 0.0008), uncorrelated with arterial partial pressure of oxygen (r = -0.6996, P = 0.0534), and positively correlated with BLA level (r = 0.9483, P = 0.0003). The pathological changes of renal tissue mainly included the degeneration and necrosis of renal tubular epithelial cells, which worsened as the time went on and appeared less severe at 120 h.
CONCLUSIONThe HIF-1α expression in renal tissue increases significantly in the early stage of PQ poisoning, which is associated with increased BLA and SCr levels and causes upregulated expression of TGF-β that promotes renal fibrosis.
Animals ; Hypoxia-Inducible Factor 1, alpha Subunit ; metabolism ; Kidney ; metabolism ; pathology ; Male ; Paraquat ; toxicity ; Rats ; Rats, Sprague-Dawley ; Transforming Growth Factor beta ; metabolism