3.Application of transanus ilues tube in the case of left-semicolon cancer with intestinal obstruction
Xiang-Shi LU ; Jin-Xue TONG ; Xin-Shu DONG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(07):-
Objective To investigate the case of left-semicolon cancer with intestinal obstruction for the methods of one-stage resection and anastomosis.Methods The clinical data of ten patients with left-semicolon can- cer with intestinal obstruction treated by transanus ilues tube,were restrospectively analyzed from October 2004 to December 2006.Results No postoperative anastormotic leakage was found and the patients were clinically cured. Conclusion The technical problem that left-semicolon cancer with obstruction for one-stage resection and anastomo- sis was resolved by the application of transanus ilues tube.
5.A case report of varicella pneumonia, hepatitis and pancreatitis secondary to kidney transplantation
Cuixing ZHOU ; Dong XUE ; Hao LU ; Renfang XU ; Xiaozhou HE
Chinese Journal of Urology 2021;42(4):306-307
A case of varicella pneumonia, hepatitis and pancreatitis after kidney transplantation was retrospectively analyzed. One week after kidney transplantation, the patient had a papule with pruritus, which was diagnosed as varicella by dermatologist as well as high-throughput sequencing. The patient was found to have pneumonia, hepatitis and pancreatitis. The individualized treatment regimen was used, including the dosage reduction of immunosuppressive agents, the blood drug concentration monitoring, antiviral therapy, anti-infection therapy, supportive treatment, and symptomatic alleviation for complications. The treatment was adjusted according to the indicators'variation. The timely review of the indicators and immunosuppressant blood concentration were performed to protect the transplanted kidney function, and the patient recovered in time. This rare case of postoperative complications of kidney transplantation were summarized and analyzed in order to accumulate clinical experience for the treatment of renal transplantation.
6.Experimental study of recombinant adenoviral-mediated PDGF gene delivery of human umbilical cord derived mesenchymal stem cells
Chunlan DONG ; Jing GE ; Lu LIANG ; Xiaojun CHEN ; Feng XUE ; Zhongchao HAN
Journal of Leukemia & Lymphoma 2011;20(6):341-344
Objective To investigate the infect capability of recombinant adenovirus mediated PDGF to human umbilical cord derived mesenchymal stem cells (MSCs). Methods The PDGF cDNA sequence was amplified with RT-PCR and constructed recombinant adenovirus vector AdPDGF. The human umbilical cord derived MSCs were isolated and cultured. In vitro AdPDGF infected MSCs with various MOI,and determine the efficiency using FACS and fluorescence microscope. Trypan blue and MTT assay was used to detect cell viability and proliferation. ELISA was used to quantify PDGF secretion. Results Recombinant adenovirus AdPDGF was successfully and constructed,in which infection efficiency to MSCs was dose dependent. The highest efficiency was around 87.36 % when MOI =50,at which cell viability and proliferation wasn' t impaired. Cell viability of uninfected,AdPDGF infected and control virus infected MSCs was (97.8 ±2.3) %,(91.9±4.0) % and (92.8±4.0) %,separately. The cell proliferation was (100±16.8) %,(95.9±12.0) % and (87.5±9.7) %,separately. There was no statistic significance among AdPDGF infected,control virus infected and uninfected MSCs. 48 hrs post infection,PDGF secretion can be measured from infectious MSCs' supernatant. The secretion level was (1.53±0.37),(3.03±0.68) and (5.25±0.92) ng/ml,separately,when MOI= 10,30,50. No PDGF can be detected from MSC-GFP and control MSC group. Conclusion Recombinant adenovirus AdPDGF was constructed and can infect human umbilical cord derived MSC at high efficiency.
7.Application of ring-like double tubes with negative pressure drainage after radical surgery of breast cancer
Xiang-Shi LU ; Jin-Xue TONG ; Xin-Shu DONG ; Da PANG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To investigate the appropriate and pragmatic drainage after the breast cancer by radi- cal surgery.Methods 120 patients treated by the radical surgery from July 2003 to July 2005,were divided into the experimental group(ring-like double tubes with negative pressure drainage) and the control group(armpit single tube drainage with pressure bind).The differences between the two groups were analyzed in the same period,respectively sixty cases.Results The outflow of the first three days after operation in the experimental group was more than that in control group(P0.05).The incidence of sinoma and incision delaying healing in experimental group was lower than that in con- trol group(P
8.Gouty arthritis of hands and feet combined with giant calcified tophus: a report of 1 case.
China Journal of Orthopaedics and Traumatology 2009;22(12):955-955
Arthritis, Gouty
;
diagnostic imaging
;
pathology
;
surgery
;
Foot Joints
;
diagnostic imaging
;
pathology
;
surgery
;
Hand Joints
;
diagnostic imaging
;
pathology
;
surgery
;
Humans
;
Male
;
Middle Aged
;
Radiography
9.Effects of infective necrosis on poor prognosis in acute pancreatitis
Bo LU ; Huadan XUE ; Yamin LAI ; Jiaming QIAN ; Hong YANG ; Dong WU
Chinese Journal of Digestion 2017;37(4):244-248
Objective To evaluate the effects of infective necrosis (IN) on prognosis in moderately severe or severe acute pancreatitis (AP).Methods According to the revision of Atlanta classification,from January 2001 to January 2015,admitted patients with moderately severe or severe AP were retrospectively analyzed.According to whether with the presence of persistent organ failure (POF) and / or IN,the patients were divided into four groups:group one with weither IN nor POF,group two with IN but without POF,group three with POF but without IN,group four with both IN and POF.The differences in disease severity and prognosis among groups were compared.Logistic regression and Cox proportional hazard regression model were used to analyze the effect of IN on prognosis.Results A total of 375 moderately severe or severe AP patients were enrolled.There were 211,43,90 and 31 patients in group one,two,three and four,respectively.A total of 121 (32.3%) patients with POF,74 (19.7%) patients with IN,and death in 63 (16.8%) patients.The mortality rate in patients with IN was 32.4% (24/74),and which was 13.0%(39/301) in patients without IN.The mortality rates of group one,two,three and four were 1.9%(4/211),11.6%(5/43),38.9%(35/90) and 61.3%(19/31),respectively;mortality rate was in a trend of increasing,and the difference was statistically significant (x2 =109.672,P<0.01).Both IN (OR=8.24,95%CI2.09 to 32.46) andPOF (OR=8.31,95% CI2.48 to 27.87)were independent risk factors of mortality of AP patients (both P<0.01).Both IN (OR=2.04,95 %CI 1.19 to 3.48,0.002) and POF (OR=5.25,95%CI 2.36 to 11.65) also were independent risk factors of shortened survival time of AP patients (both P<0.01).Conclusions IN is an independent risk factor of disease severity and poor prognosis in AP.The prognosis is the worst in AP patients with both POF and IN.
10.Microvasculature change in the diagnosis of early esophageal carcinoma using magnifying endoscopy
Rui JI ; Xue-Feng LU ; Jin-Dong FU ; Yan LUO ; Yu-Juan WANG ; Li-Rui TU
Chinese Journal of Digestive Endoscopy 1996;0(06):-
Objective To study the detection rate of early esophageal carcinoma using magnifying endoscopy,and to evaluate the relationship between the imaging patterns of microvasculature change and his- tological diagnosis.Methods Two hundred and fourteen patients with esophageal mucosa roughness,ero- sion,plaque,abnormal color and indentation in conventional endoscopy and 16 healthy volunteers were en- rolled.The magnifying endoscopy images were graded as four patterns by intraepithelial papillary capillary loop(IPCL)changes after iodine dyeing.The biopsies underwent pathologic evaluation.The comparison be- tween the imaging patterns of endoscopy and histological diagnosiswas was evaluated.Results 80.4%(90/ 112)esophagitis was type 2,and 85.7%(12/14)early esophageal carcinoma was type 3 and type 4.The difference was significant between early esophageal carcinoma and normal mueosa(?~2=27.32,P