Objective To explore the diagnostic value of LUNX gene to marrow micrometastases of lung cancer.Methods To detect LUNX mRNA of marrow samples of 51 patients of lung cancer,4 patients of breast cancer,6 patients of lymphadenoma,3 patients of liver cancer and 22 patients of benign disease by real-time RT-PCR.Results The positive detection rate and meso-copies of lung cancer were 58.8% (30/51) and 35copies/ml respectively.The positive detection rate and meso-copies of other diseases were all 0,The positive detection rate and meso-copies of lung cancer was significantly higher than that of patients with other diseases (x~2=11.12,U_c=3.7329,P

In traditional clinical application, Coptidis Rhizome and Evodiae Fructus have been combined to treat various stomach heat and cold syndromes, gastritis, gastric ulcer and the like. With the application of modem instruments and the development of molecular pharmacologic theory, their chemical constituents and pharmacological effects have been sufficiently studied. In this paper, literatures from Pubmed were adopted, with particular emphasis on findings of international counterparts and studies on compatibility of main chemical components in Coptidis Rhizoma and Evodiae Fructus, in order to elaborate on the scientific comparability of Coptidis Rhizoma and Evodiae Fructus through chemical analysis, and pharmacological and biopharmaceutics studies and introduce the future development trend of the studies.
Animals ; Drug Interactions ; Drugs, Chinese Herbal ; analysis ; pharmacology ; Evodia ; chemistry ; Fruit ; chemistry ; Humans ; Ranunculaceae ; chemistry ; Rhizome ; chemistry

Based on the Bacillus sp., isolated from Lop Nur Desert, the technology of separation and purification and the antioxidant effect were studied. After centrifugation and vacuum filtration, the deproteinization of supernatant was operated with Sevag reagent. The crude exopolysaccharide (EPS) was obtained by precipitation with ethanol. The optimum conditions for the isolation were as follow: pH 7.0, temperature 4?C, time 1.5 h, and material to ethanol ratio 1: 4. Dissolved in water, the crude EPS was fractional separated on activated carbon column (1.5 cm ? 24 cm), eluted with distilled water, 60% ethanol, 95% ethanol, and the main fraction was collected. Then the EPS was purified on Sephadex G-100 gel column, eluted with NaCl (0.2 mol/L). Fractions (4 mL, each) were also combined according to total sugar by phenol-sulfuric acid method and protein content was determined by Coomassie brilliant blue. The results showed that EPS was relatively homogeneous glycoprotein. The data of antioxidation in vitro showed that the EPS had a high antioxidant activity, which could quench hydroxyl radical, superoxide radical and had antilipid peroxidation activity. All of these indicated that EPS was a good natural antioxidant.

Objective To observe the MRI features of traumatic brainin jury to corpus callosum and provide valuable diagnostic information for clinical application. Methods Clinical and radiological data were retrospectively analyzed in 20 MRI-diagnosed patients as having traunmtic brain mjury to corpus callosum. Results Traumatic injury to corpus callosum was rather rare in brain trauma.Most traumatic injury to corpus callosum was non-hemorrhagic and its lesion mainly laid at its body or splenium,sometimes at genu,but rarely at rostrum.Traumatic injury to corpus callosum,being one of the common manifestations in diffuse axonal injury(DAI), could be demonstrated slight hyperintense signal on TI WI and T2WI in early phase.The injury site shrank and liquefaction necrosis appeared in the lesion at the late-mid-injured period with softened focus or gliai scar.MRI showed hyperintense T1 and T2 signal, similar to the signal of the cerebrospinal fluid and demonstrated ventriculomegaly in the corresponding parts.DWI showed hyperintense signal in the lesion in acute and subacutc stages:with time prolonged,isointense signal of the normal brain tissue was found and signal of the cerebrospinal fluid appeared in the present of softened focus.Conclusion Attention should be paid to the traumatic injury to corpus callosum,an indicator for severe trauma or disease.MRI,a sensitive and multi-directional imaging,is superior in detecting non-hemorrhagic injury to the corpus callosum.

Objective To measure the bone mineral density(BMD)of mandible and investigate the relationship between mandible and the whole body skeleton BMD.Methods Healthy volunteers were recruited in north China,which were divided into 6 groups by age:≥20,≥30,≥40,≥50,≥60 and ≥70 years older,10 male and 10 female in each group.Dual-energy X-ray absoptiometery(DXA)was used to measure the BMD of the lumbar spine,the mentum of mandible and the mandibular angle.The results were analyzed statistically.Results The mineral density(MD )of the mentum was(1.310 9 ±0.035 5)g/cm2,the left mandibular angle(1.048 9 ± 0.013 7)g/cm2,the right mandibular angle (1.0547 ±0.014 1)g/cm2,the lumbar spine(L2-L4)(1.121 1±0.017 2)g/cm2.There was a significant difference in mandibular angle and lumbar MD between men and women(P＜0.05).The MD of mandibular angle and lumbar spine decreased significantly after 50 years of age.Conclusions The normal BMD of the mandibular mentum,mandibular angles and lumbar spine is obtained.The BMD of the mandibular angles is closely related to that of the lumbar spine.Mandible can be an appropriate measurement site in the evaluation of skeleton BMD status for the forecast of osteoporosis.

Objective To investigate the correlations of altitude and duration on the plateau with the heart rate and oxygen saturation of young males.Methods A total 483 cases of males garrisoned on the plateau were enrolled.Informa-tion on age, underlying diseases, length of service on the plateau and garrison areas was collected.The heart rate and oxygen saturation were measured,and the correlations of altitude and duration on the plateau with the heart rate and oxygen saturation were analyzed.Results There was no statistically significant difference in oxygen saturation between the group stationed on the plateau for more than 6 years and the group stationed for less than 6 years.There was significant difference in blood oxygen saturation and heart rate between the two groups.Oxygen saturation was negatively correlated with altitude. Heart rate was positively correlated with altitude.Conclusion Young males who are stationed more than 4500 m above sea level are at high risk of chronic high altitude disease.The risk of chronic high altitude disease can increased with the length of service and age,but further confirmation is meeded.

Objective To detect the expression levels of high mobility group box chromosomal protein 1 (HMGB1) and Th17 cells transcription factors, related cytokines in peripheral blood of rheumatoid arthritis (RA) patients and analyze the relations between HMGB1 and CRP, ESR, RF in RA patients. The other aim of this study is to identify the expression level of HMGBI and the relationship between HMGB1 and Th17 in RA patients. Methods The mRNA levels of HMGB1, RORyt, interleukin (IL)-17 in the peripheral blood mononuclear cells (PBMC) were determined by quantitative real-time PCR (QRT-PCR) from 80 patients with rheumatoid arthritis,including 32 RA patients in stable phase and 48 patients in active phase, and 50 healthy volunteers. The concentration of HMGB1, IL-23, IL-17 in plasma were detected by enzyme linked immunosorbent assay (ELISA), one-way ANOVA and Spearman's correleation were adopted for statistical analysis.Results The mRNAs of HMGBI, RORyt and IL-17 in RA patients were higher than that in healthy control group (P<0.05), especially in active RA patients [ HMGB 1 (0.424±0.262) pg/ml, RORγt (0.34±0.25) pg/ml,IL-17 (1.42±0.38) pg/ml,P<0.01 ] when compared with patients with stable disease. The concentration of HMGB1, IL-23 and IL-17 in the plasma of RA patients was higher than that of the healthy control group (P< 0.05), and was positively correlated with the expression levels of HMGB1, Th 17-associated factors and the level of CRP, ESR, RF in RA patients' plasma(P<0.05). Conclusion The HMGB1 and Thl7 cells levels are higher in active RA patients than those in patients with stable disease, arid there is significant positive correlation between them. Detection of peripheral HMGB1 and Thl7 cell-specific transcription factors or related cytokines can help to understand the development and progress of rheumatoid arthritis and provide clues for new treatment targets for RA.

Objective To provide the skin vessels morphologie basis for perforating flap of the antero- lateral thigh(ALT).Methods Six sides lower limbs of adult fresh cadaver specimens perfused with lead oxide-gelatine mixture were used.Observe the peraforators of anterolateral thigh by dissection.The elevated tissue was radiographed by X-ray and MSCT-scanning.Computer techniques was used for the detection and 3D-reconstruction of the regions of each perforators of ALT flap.Results The three-dimensional recostruct- ed digitized visible models perfectly displayed the anatomic structures of arteries on the anterolateral thigh, and,the morphology and distribution of the vascular territory of each perforator from descending branch of LCFA was displayed in the images of 3D-visualization,their effective morphology and distribution were same, and they accord with vascular territory of radiograph.On the 2D-image of X-ray,The margins of the anatomical distribution of each perforator from descending branch of the lateral circumflex femoral artery were determined, their total areas was about 25cm?24cm.Conclusion Since the digitized image of perforator territory can accord with the morphology of anatomy,and can off vascular structure insights into cutaneous perforator anato- my,their 3D visualizative models can be applied in pre-operative designing and virtual operation procedures, and can be helped for study of perforator flap.

Objective To evaluate the effect of therapeutic communication on anxiety caused by diagnosis of suspicious malignant tumor by PET-CT.Methods Totals of 55 anxious patients diagnosed of suspicious malignant tumor by PET-CT were enrolled in the study.All the patients received routine clinical examination and therapeutic communication.The self-roting anxiety scale was used to conducted the anxiety situation of patients before intervenfion and after two weeks intervention.Results After the intervention,the patients ' anxiety scores were significantly lower than that before [(57.1 ±9.7) vs (38.6 ±7.2),t =12.5,P ＜0.01].Conclusions Therapeutic communication can effectively relieve the anxiety of patients caused by diagnosis of suspicious malignant tumor by PET-CT.

Objective To establish a high performance liquid chroma-tography-tandam mass spectrometry ( HPLC-MS/MS) method for de-termining the concentration of sunitinib and N -desethyl sunitinib (SU12662) in human plasma.Methods Sunitinib, SU12662 and in-ternal standard sunitinib -D10 were extracted from plasma with one -step protein precipitation and the stereoselective analysis of sunitinib and SU12662 was achieved on an Agilent ZORBAX Extend C18 (2.1 mm ×75 mm ×3.5 μm) with gradient elution by a mobile phase consisting of wa-ter containing 0.1%formic acid and 95%acetonitrile.Electrospray ioni-zation source was applied, and multiple reaction monitoring mode was operated in the positive mode with the monitor ions at m/z 399.3→326.1 for sunitinib, m/z 371.3→283.1 for SU12662 and 409.3→326.2 for sunitinib-D10.Results The calibration curve for plasma sunitinib was linear in the range of 0.5-200 ng? mL-1 ( r=0.998 7) , the lower limi-tation of quantification was 0.5 ng? mL-1 , while the accuracy was ranged from 0.57% to 2.87%, the average recovery rate was ranged from 92.75%to 98.62%,and intra-and inter-day RSD were ranged from 0.91%to 1.92%and 5.40%to 7.87%, respectively.The calibration curve for plasma SU12662 was linear in the range of 0.25 -100 ng? mL-1 ( r =0.999 9 ) , while the accuracy was ranged from -2.08% to 2.80%, the average recovery rate was ranged from 96.23%to 101.12%, and intra-and inter-day RSD were ranged from 0.46%to 2.46%and 5.84%to 9.75%, respectively, the lower limitation of quantification was 0.25 ng? mL-1 .Conclusion The HPLC-MS/MS method was accurate, sensitive, specific, and could be used in the determination of sunitinib in plasma and the clinical study of its pharmacokinetics.