1.Correlation research between prognosis of NSCLC patient and serum levels of HGF and IL-6
Zhen SUN ; Ying XUE ; Dongxia WANG ; Ying WANG
Journal of Regional Anatomy and Operative Surgery 2015;(4):403-405
Objective To study the correlation between prognosis of non-small cell lung cancer ( NSCLC) patient and serum levels of HGF and IL-6. Methods Eligible 109 NSCLC patients and preoperative blood samples of each patient in our hospital from March 2013 to May 2014 were collected. Then the levels of HGF and IL-6 of serum samples were detected by using ELISA kit. All patients were followed up for 1 year. The neoplasm staging:67 cases were stagingⅠ-Ⅱ,38 cases were stagingⅢ,2 cases were staging IV. There were 79 cases with ad-enomatous carcinoma( ADC) ,26 cases with squamous carcinoma( SCC) and 4 cases with NSCLC. According to the TNM staging,there were 79 cases in pN( -) group,31 in pN( +) group,77 in pT(T1-T2) group and 28 in pT(T3-T4) group. Results The average concentration of HGF and IL-6 in serum were 860 pg/mL and 2. 7 pg/mL respectively. Analysis of survival indicated that,compared to those patient with lower serum level of HGF and IL-6,the survival rate of patient with high serum level was much lower. The difference was statistically signifi-cant (HGF,P=0. 019;IL-6,P=0. 002). The analysis of the patients with stageⅢdisease separately also indicated that survival rate of pa-tient with lower serum of HGF and IL-6 was higher than others. Conclusion The serum levels of HGF and IL-6 might be a effective indica-tors for predicting prognosis of the NSCLC paitents.
2.Study on protective effect of Shenmai injection on hematopoiesis of bone marrow in acute leukemia patients with chemotherapy.
Jian-ying WANG ; Xue-mei ZHONG
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(3):266-267
Adolescent
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Adult
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Aged
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Antineoplastic Combined Chemotherapy Protocols
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adverse effects
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therapeutic use
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Drug Combinations
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Hematopoiesis
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drug effects
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Humans
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Leukemia, Myeloid, Acute
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drug therapy
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Male
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Middle Aged
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Phytotherapy
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Schisandraceae
3.Association of PD-1 expression on CD4~+CD25~(nt/hi)CD127~(lo) regulatory T cells with disease progression in HIV-1 infected patients
Qinghua CAO ; Yile XUE ; Ying WANG
Journal of Cellular and Molecular Immunology 2009;25(11):1020-1022
AIM: To investigate whether Programmed death-1 (PD-1) expression on peripheral CD4~+CD25~(nt/hi)CD127~(lo) regulatory T cells (Treg) was associated with disease progression in HIV-1-infected patients. METHODS: Peripheral blood from 108 HIV-1-infected patients in distinct disease progression statuses and 27 healthy individuals were collected in the present investigation. PBMCs were isolated by centrifugation on Ficoll-Hypaque, followed by staining with anti-CD4-PerCP, anti-CD25-FITC, anti-CD127-PE and anti-PD-1-APC. PD-1 expression on Treg was analyzed by four-color staining flow cytometry. CD4~+T cell absolute counts were determined using Multitest CD3/CD4/CD8/CD45 kit and plasma viral loads were detected on NucliSens EasyQ. All data were analyzed using SPSS14.0 software. RESULTS: In peripheral blood of healthy individuals, Treg expressed PD-1 at very low levels (1.72%±0.65%). In contrast, Treg from HIV-1-infected patients showed a significantly increased PD-1 expression (5.33%±2.24%, P<0.01). Moreover, AIDS patients exhibited statistically higher PD-1 expression on Treg (7.87%±2.23%) than newly HIV-1 infected patients (3.22%±1.01%, P<0.05) and patients in progression to AIDS(5.21%±1.72%, P<0.05). PD-1 up-regulation on Treg was closely correlated with reduced CD4~+T cell absolute counts but elevated plasma viral load. CONCLUSION: Overall, we found that PD-1 expression on peripheral Treg was up-regulated and correlated with disease progression in HIV-1-infected patients for the first time. These findings not only extend our understanding of how Treg functions in HIV-1-infected patients but also support the notion that blocking PD-1/PD-L1 interactions may represent a potential therapeutic strategy for HIV-1-infected patients.
5.Case of bilateral diaphragm paralysis.
Kai-xue JIA ; Ying-hui WANG ; Cong-huo TIAN
Chinese Acupuncture & Moxibustion 2011;31(6):565-566
Acupuncture Therapy
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Diaphragm
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pathology
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Humans
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Male
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Middle Aged
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Respiratory Paralysis
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pathology
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therapy
7.Effect of iohexol on the renal function in children undergoing cardiac angiography.
Xin-Ying ZHANG ; Yu-Lin WANG ; Xue-Lian ZHU
Chinese Journal of Contemporary Pediatrics 2009;11(11):935-936
Child
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Child, Preschool
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Contrast Media
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adverse effects
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Coronary Angiography
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Female
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Humans
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Infant
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Iohexol
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adverse effects
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Kidney
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drug effects
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physiology
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Male
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beta 2-Microglobulin
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urine
8.Evaluation of nocturnal hypoglycemia in patients with type 2 diabetes via continuous glucose monitoring system
Yan WANG ; Shouping GUAN ; Ying KANG ; Yuanming XUE
Chinese Journal of Endocrinology and Metabolism 2010;26(5):393-394
The variation of blood glucose in 1 147 patients with type 2 diabetes mellitus was monitored by continuous glucose monitoring system(CGMS). The results showed that the peak hours of nocturnal hypoglycemia occurred during 22 : 00-2 : 00. The incidence of nocturnal hypoglycemia was negatively correlated with mean blood glucose and blood glucose at three hours after supper. The probability of hypoglycemia was up to 50% when blood glucose level had been as low as 4. 7 mmol/L at 3 h after supper.
9.Establishment of mice model of C6 glioma stem cells and its evaluation
Yahua WANG ; Haolun XU ; Helu YAN ; Xia LI ; Xue YING
Chinese Pharmacological Bulletin 2016;32(11):1620-1622
Aim To establish ICR animal model with C6 glioma stem cells, to provide the ideal model for the further study of gli-oma stem cells in brain glioma model. Methods C6 glioma stem cell was cultured in vitro by suspension,and was identified with Nestin antibody. C6 stem cells of ICR mouse glioma model were used to investigate survival state and tumor volume in mice after the operation. HE staining and CD133 immunohistochemi-cal study were adopted to investigate the postoperative pathologi-cal changes in mice. Results The expression of Nestin was 96. 01% in C6 glioma stem cells, and Nestin was highly ex-pressed in the cultured C6 glioma stem cells. Mice were inocula-ted with tumor after loss of appetite, weight, behavior and slow, sluggish reaction. Tumor volume at day 21 after modeling was (9. 77 ± 6. 58) mm3 . After HE staining, the model showed the invasive growth, tumor cell shrinkage and derangement. Immu-nohistochemical CD133 staining revealed that tumor cytoplasm color was brown. Conclusion Glioma model can be established based on glioma stem cells into a high rate of tumor, the tumor cycle is short, which can be used as an ideal model for glioma.
10.Effects of Chinese herbal medicine Yiqi Zengmin formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats.
Hongli XUE ; Zeng ZHANG ; Wenjian WANG ; Weihua CHEN ; Jian YING
Journal of Integrative Medicine 2011;9(10):1133-7
To investigate the effects of traditional Chinese compound recipe Yiqi Zengmin (YQZM) formula on expression of glucose transporter 4 (GLUT4) in skeletal muscle of rats with type 2 diabetes induced by high-fat diet combined with low-dose-streptozotocin injection.