The S100 proteins are a multi-gene calcium-binding family,which are differently expressed in a variety of tumors.It is found to be associated with tumor invasion and metastasis.As a tumor-associated biomarkers,unraveling the relationship between S100 and prostate cancer progression as well as molecular mechanisms,would provide an important basis for the clinical diagnosis and therapeutic monitoring.

Video assisted thoracic surgery(VATS) was rapidly developed in recent years.It's been widely accepted because it was minimally invasive and the patients were fast recovery with a survival rate similar to routine thoracotomy patients.However,more and more attention was paid to the side effect of general anesthesia with double cavity intubation during the surgery.Nonintubated thoracoscopic surgery for lung cancer was progressively developed.This surgery method decreases the side effect compared to general anesthesia with double cavity intubation.However,more surgical difficulty,additional surgical risk and prolonged surgery time were all inevitable become disadvantages of this new method.The less side effect of general anesthesia for patients and the more challenge for thoracic surgeon was brought simultaneous by this new method.More clinic trial should be implemented in validating the advantages and disadvantages.

Objective To discuss the expression characteristic of high-sensitivity C-reactive protein(hs-CRP) in coronary heart disease.Methods 115 patients of coronary heart disease confirmed by coronary angiography were randomly recruited, including 42 cases of acute myocardial infarction(AMI),34 cases of unstable angina pectoris(UAP) and 39 cases of stable angina pectoris(SAP). Another 50 patients of healthy persons were recruited as control group. Their levels of serum hs-CRP were determined by particle enhanced immuoturbidimetric method at the analysis instrument of Olympus AU2700.Later the variation of hs-CRP before and after treating was analysed.Results hs-CRP concentration of CHD patients was significantly higher than that of the control group(P

BACKGROUND: In previous studies on tissue-engineered adipose, the pore size of scaffolds is easily neglected. If the pore size was large, cells would flow away along the pore and could not maintain in the scaffold. If the pore size was small, cells would distribute on the surface of the scaffold, and could not enter the scaffold. Simultaneously, it would be not contribute to growth of new vessels. OBJECTIVE: To screen appropriate silk fibroin porous scaffolds to construct tissue-engineered adipose. METHODS: Under unchanged concentration of flbroin, six kinds of silk fibroin porous scaffolds in different aperture were prepared by changing freezing and drying temperature and time. Human umbilical cord mesenchymal stem cells (hUCMSCs) were harvested using attachment method. The potentiality of its osteogenic and adipogenic differentiation was detected by chemistry staining. The apertures of six kinds of silk fibroin porous scaffolds were measured by scanning electron microscope. The adhesion and proliferation of hUCMSCs on silk fibroin porous scaffolds in different aperture were observed. RESULTS AND CONCLUSION: The apertures of six silk fibroin porous scaffolds were (39.94±17.27), (53.51±16.18), (63.97±19.76), (71.08±18.07), (87.33±21.78), (121.97±44.10) μm, respectively. The strong ability of attachment of hUCMSCs appeared in the number two scaffold, and in other materials (number 1,3,4,5,6), there were not any cell in them except number one and three. Therefore, 50 μm might be a suitable aperture to construct tissue-engineered adipose with silk flbroin porous scaffolds using hUCMSCs.

BCG Vaccine ; adverse effects ; Humans ; Infant ; Male ; Mycobacterium bovis ; Severe Combined Immunodeficiency ; complications ; Tuberculosis ; complications

Wheezing is the most common respiratory disease in children.In recent years,the incidence of childhood wheezing showed an upward trend,the hot topic in the current study is how to draw up a rational and effective treatment to reduce wheezing.This paper summarized the latest research progress of pathogenic factors,clinical classifi-cation,pathogenesis and prevention strategies in childhood wheezing.It aimed to provide a theoretical basis for early diagnosis and individual treatment for children with wheezing diseases.

Factor Analysis, Statistical ; Health Policy ; Leadership ; Occupational Health Services ; organization & administration

Objective To explore the dynamic changes of collagen type Ⅰand Ⅳ messenger ribonucleic acid(mRNA)expression in the lung tissue of neonatal rats after inhaling high concentration of oxygen and the role of collagen type Ⅰand Ⅳ mRNA in chronic lung disease(CLD)induced by hyperoxia.Methods Full-term newborn rats were grouped according to inhale the concentration of oxygen into hypero-xia group and air control group after birth within 12 hours.Lung histological section at day 1,3,7,14 and 21 in 2 groups were prepared for hematoxylin-eosin staining and the detection of mRNA level of collagen type Ⅰand Ⅳ by in situ hybridization.The results were analyzed with SPSS 11.0 software.Results Compared with air control group,inflammation response was seen in early stage,the arrest of lung development was evident after 7 d of oxygen exposure,at last interstitial fibrosis.It was shown that the positive expression of collagen typeⅠ was mainly in the alveolar epithelial cells and endothelial cells by in situ hybridization.The expression of collagen typeⅠ mRNA was weakened compared to air group on 7 d(P0.05).Conclusions Prolonged hyperoxia may cause the onset of arrested lung development and lung fibrosis,which are similar to the changes of chronic lung disease.The collagen type Ⅰand Ⅳ mRNA expressions are not parallel to their protein contents,suggesting the main modulation of these collagens may be not at transcriptional level.

This article described systematically the design basis,passing standard and influential factor of test score of the assessment scale of simulated clinical skills examination.Through developing and modifying OSCE examination scale design for many years,we have found that the use of 360-degree assessment for designing OSCE assessment rating scale can effectively reduce the impact on the score of examinee owing to the environment,the candidates,raters and question design,which has an important role for scientific development of OSCE assessment scales,as well as improving the reliability and validity for development of a scientific OSCE assessment.

Objective To investigate the clinical therapeutic effect of Chaishi fever particles on patients with epidemic encephalitis B and to study the machanism of anti-inflammatory.Methods One hundred and twenty one patients with epidemic encephalitis B admitted to the Second Clinical Medical College of Henan University of Chinese Medicine from January 2014 to May 2017 were enrolled, and they were divided into a routine treatment group (60 cases) and a traditional Chinese medicine (TCM) treatment group (61 cases) by random number table. The patients in routine treatment group were given symptomatic support and comprehensive treatment for 15 days, while those in the TCM treatment group were given as that of routine group with the addition of Chaishi fever particles 8 g, 4 times every day, orally taken for consecutive 15 days. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL-1β, IL-10) in the serum of two groups were determined by enzyme-linked immunosorbent assay (ELISA), and the clinical therapeutic effect, complications and sequelae in two groups after treatment were observed.Results Compared with the routine treatment group, after treatment the time of body temperature recovering normal (days: 5.8±1.2 vs. 7.5±1.7), the coma time (days: 5.5±1.3 vs. 6.8±1.6), the remission time of convulsion (days: 5.2±1.4 vs. 6.5±1.5), and the length of stay in hospital (days: 22.6±1.9 vs. 25.2±1.8 ) were significantly shorter in TCM treatment group (P < 0.05). After treatment, the levels of serum tumor TNF-α and IL-1β were decreased, IL-10 was increased in both groups, moreover, compared with the routine treatment group, the changes were more obvious in TCM treatment group [TNF-α (μg/L): 11.4±3.6 vs. 14.6±3.5, IL-1β (μg/L): 22.3±6.2 vs. 26.2±5.6, IL-10 (μg/L): 225.2±19.2 vs. 186.2±21.5, allP < 0.05]. In addition, compared with the routine treatment group, the total effective rate was higher [98.3% (60/61) vs. 90.0% (54/60),P < 0.05], and the incidence of sequelae was lower [1.64% (1/61) vs. 13.3% (8/60),P < 0.05] in TCM treatment group.Conclusion The Chaishi fever particles can effectively treat the patients with epidemic encephalitis B and the action is related to the inhibition of inflammatory reaction.