The incidence of gastric cancer is in the fourth place of all cancers in worldwide ,and the fatal-ity rate is in the second place .Standard chemotherapy regimens play an important role in prolonging gastric cancer survival rate.However,most of gastric cancers are in advanced stage when diagnose is finally made .The current medical practice can only meet the limited treatment options .In the last several decades ,study of the molecular mechanisms of gastric cancer gets constant new progress .By inhibiting the human epidermal growth factor receptor 2(Her-2)for advanced gastric cancer,it provides a new treatment for patients.Trastuzumab is a kind of Her -2 monoclonal antibody .It is the first kind of targeted therapy drug for the treatment of metastatic gastric cancer .A combination of trastuzumab with cisplatin and 5-fluorouracil based chemotherapy benefits gastric cancer overall survival.Overexpression of Her -2 provides evidence for the targeted therapy .So we reviewed Her -2 excessive expression of gastric carcinoma in recent years and represented by bead sheet resistance to targeted therapy .In this paper ,research of overpression of Her -2 and targeted threrapy in gastric cancer are summarized .

Objective To study the state of oxidative stress induced by sodium arsenite (NaAsO2) in SV-HUC-1 cell. Methods MTT assay was used to evaluate the viability of the cells. The level of ROS was detected by staining cells with DCFH-DA. The content of GSH and MDA were measured by DTNB and thiobarbituric acid methods. The activity of SOD was measured by xanthine oxidase method. Results Compared with the control group, the viability cells decreased in all the treated groups (P0.05), the activity of SOD in all the treated groups was significantly decreased (P

Objective The aims of this study were to investigate the prognostic value of CEA and CA19-9 expression in advanced gastric cancer and the prognostic factors of advanced gastric cancer.Methods A total of 255 patients with advanced gastric cancer who underwent radical R0 radical surgery were enrolled in the Department of Gastrointestinal Surgery,Harbin Medical University Cancer Hospital from January 2010 to December 2010,and were divided into group A(CEA<2.19ng/mL and CA19-9<10.78U/mL),group B(CEA≥2.19ng/mL or CA19-9≥10.78U/mL)and group C(CEA≥2.19ng/mL and CA19-9≥10.78U/mL).These groups were analyzed the differences in gastric cancer patients with clinical pathology and survival,and prognostic factors of patients with advanced gastric cancer factors.Results There were statistically significant differences in clinical N staging,clinical TNM staging and pathological TNM staging in three groups of patients with advanced gastric cancer(P<0.05).There was no significant difference in the median survival time in three groups of patients with advanced gastric cancer(P>0.05).The 1-year,3-year and 5-year survival rates of group A were higher than those of group B and group C(P>0.05).The factors affecting the prognosis of gastric cancer included the degree of tumor differentiation,pathological T staging,pathological TNM staging,the total number of lymph nodes,the number of positive lymph nodes and lymph node metastasis.Conclusion Combined detection of CEA and CA19-9 expression indicates a certain reference value for prognosis of gastric cancer.

Objective To observe the protective effect of the separate zinc gluconate oral and combined of Salvia injection on whole blood metal ion of calcium,magnesium,iron,copper and zinc concentration in noise-induced rats.Methods 50 female SD rats were randomly divided into a control group,a noise group,a zinc gluconate noise group (plus zinc group),a Salvia injection noise group (plus Salvia group) and a Zinc gluconate oral liquid and Salvia injection noise group (combined group),with10 rats in each group.Except the control group did not expose to noise,the rest groups were continuously exposed to high frequency steady noise for two weeks.Each group was compared for the concentration differences of whole blood metal ion of calcium,magnesium,iron,copper and zinc after the intervention of noise.Results ① In each group at the comparison of the calcium ion concentration: Calcium ion concentration of the control group(1.25± 0.16)mmol/L and the combination group(1.27 ± 0.10) mmol/L was significantly lower than the noise group (1.42 ± 0.18) mmol/L.The rest groups compared to each other were not statistically significant.②Magnesium ion concentration was highest in the noise group (1.53 ± 0.10)rmtmol/L),and lowest in the control group (130 ± 0.29) mmol/L,and the noise group was significantly higher than that of the control group.The mean of magnesium ion concentration in plus zinc group (1.42± 0.27) mmol/L,plus Salvia group (1.38± 0.15) mmol/L and combined group(l.37±0.11)mmol/L were lower than the noise group,but the difference was not significant (P＞0.05).③ The iron ion concentration of the noise group (5.47± 1.29)mmol/L was significantly lower than the other four groups (P＜0.05).The control group,plus zinc group,plus Salvia group,the combined group showed no significant differences.④ Whole blood copper ion concentration of the noise group (16.69 ± 4.18) μmol/L was significantly lower than the control group (21.53 ± 3.78) μmol/L and the combination group(19.53± 1.92)μmol/L with a statistical difference; compared with the control group,the concentration of copper ions in plus zinc group(16.19± 1.93)mμol/L was significantly lower (P＜0.05).⑤The whole blood zinc ion concentration in the noise group (50.83±7.99)μmol/L was significantly lower than the other groups,zinc ion concentration in the plus Salvia group (53.87±6.77)μmol/L was significantly lower than the control group (63.86± 8.83) μmol/L; the whole blood zinc ion concentration showed no difference between the plus zinc group (54.81 ± 5.90) μmol/L,plus Salvia group and combined group (59.21 ± 3.90) μmol/L.Conclusion Combined zinc gluconate oral solution and Salvia injection had protective effect on whole blood metal ion concentration affected by noise.The protection effect of zinc gluconate oral solution and Salvia injection combination was stronger than any individual.

Objective To observe the protective effect of the zinc gluconate combined with Salvia injection on noise induced serum immunoglobulin(IgG,IgM,IgA) and immune molecules (IL-2,IL-4) in rats.Methods 50 female SD rats were randomly divided into control group,noise group,zinc gluconate noise group(plus zinc group),Salvia injection noise group (plus Salvia group) and Zinc gluconate oral liquid and Salvia injection noise group(combined group),10 rats in each group.Except the control group,rats in the other groups were continuously exposed to high frequency steady noise for two weeks.The concentrations of serum immunoglobulin (IgG,IgM,IgA) and immune molecules (IL-2,IL-4) after the intervention of noise were compared.Results Serum levels of IgG,IgM,IL-2,IL-4of the control group were significantly higher than the noise group (all P ＜ 0.05),plus zinc group (all P ＜ 0.05),plus Salvia group(all P ＜0.05),the differences were statistically significant.Serum level of IgA of each group was not statistically different compared with control group (all P ＞ 0.05).Serum levels of IgG,IgM,IL-2,IL-4 in the noise group were significantly lower than those in the control group (all P ＜ 0.05),combined group (all P ＜ 0.05),the differences were statistically significant.Serum levels of IgG,IgM,IL-2 in plus zinc group were significantly higher than the noise group(all P ＜ 0.05).Serum IgA concentration of each group showed no significant difference compared with noise group(all P ＞ 0.05).Conclusion Zinc gluconate oral solution and Salvia injection have protective effect on immune system affected by noise in rats,the protective effect of combination of zinc gluconate oral solution and Salvia injection is stronger than the individual.

OBJECTIVE:To optimize the prescription and preparation of matrine liposomes.METHODS:The liposomes were prepared by the reverse-phase evaporation technique;the envelope efficiency and the diameter of matrine liposomes were taken as criteria,the prescription and preparation of the liposomes were optimized by orthogonal design.RESULTS:The best prescription consisted of egg phosphatidylcholin(80mg),the oil phase cholesterol(15mg),the water phase phosphate buffer so?lution(pH=7.0)and matrine(30mg);the best preparation technics was as follows:chloroform(30ml),ultrasonic time was10min rotary evaporating temperature at48℃.The liposomes had homogeneous shape with the particle size at250nm～750nm and the envelope efficiency of matrine liposomes at47.25%.CONCLUSION:The optimized formulation of martine liposomes is reasonable in prescription,practicable in technics,high in envelope efficiency and perfect in stability.

Objective To observe the effect of Jisheng injection(JI)in preserving isolated heart of mice.Methods Langendorff' s isolated -perfused mouse heart mod el was established.The isolated hearts were preserved in modified Eu-ro -Collins solution(MECS)and MECS with JI added for 3h,7.5h and8.5h respectively.Cardiac functio n,coronary flow and myocardial water -volume (MWV)were observed after 3h ,7.5h and 8.5h of preservation,heart reactivatio n(HR)rate,coronary flow,MWV and time for HR were also observed.Results After 3h of preservation and 20min,30min or 40min of reperfusion,cardiac function and coronary flow in three M ECS +JI groups and especially in that of large dose were improved as compared with t hose in MECS group;MWV in large -dose and moderate -dose groups was obvi-ously lower than that in MECS group(P

Objective To investigate the effects of zinc gluconate combined with Danshen injection on the plasma lipid level following exposure to noise in rats. Methods Fifty female SD rats were randomly divided into a control group, a noise exposure group (model group), a zinc gluconate + noise exposure group (zinc gluconate group), a Danshen injection+noise exposure group (Danshen injection group) and a zinc gluconate+Danshen injection+noise exposure group (combination group), 10 rats in each group. The zinc gluconate group received intragastric administration of zinc gluconate 8-10 mg/(kg?d), the Danshen injection group received intraperitoneal injection of Danshen injection 6 mg/(kg?d), the combination group received intragastric administration of zinc gluconate 8-10 mg/(kg?d) and intraperitoneal injection of Danshen injection 6 mg/(kg?d). On the day 10, except in the control group, the rats in the rest of the groups were continuously exposed to high frequency steady noise for 7 days (4 000 Hz, 95 dB, 3 h/d). The serum levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were detected. Results The serum levels of TC (1.88 ± 0.17 mmol/L vs. 1.55 ±0.09 mmol/L), TG (1.45 ± 0.38 mmol/L vs. 1.01 ± 0.27 mmol/L), LDL-C (0.29 ± 0.04 mmol/L vs. 0.23 ± 0.03 mmol/L) in the model group were significantly lower than those in the control group (P<0.01 or P<0.05), while the serum HDL-C level in the model group were significantly lower than that in the control group (0.55 ± 0.08 mmol/L vs. 0.62 ±0.06 mmol/L;P<0.01). The serum TC level in the zinc gluconate group was significantly lower than that in the model group (1.71 ± 0.11 mmol/L vs.1.88 ± 0.17 mmol/L;P<0.05). The serum levels of TC (1.73 ± 0.21 mmol/L vs. 1.88 ± 0.17 mmol/L) and LDL-C (0.25 ± 0.03 mmol/L vs. 0.29 ± 0.04 mmol/L) in the Danshen injection group were significantly lower than those in the model group(all P<0.05). The serum levels of TC (1.57 ± 0.21 mmol/L vs. 1.88 ± 0.17 mmol/L), TG (0.84 ±0.40 mmol/L vs. 1.45 ± 0.38 mmol/L) and LDL-C (0.24 ± 0.05 mmol/L vs. 0.29 ± 0.04 mmol/L) in the combination group were significantly lower than those in the model group (P<0.01 or P<0.05), and the serum HDL-C level (0.61 ± 0.07 mmol/L vs. 0.55 ± 0.08 mmol/L) in the combination group was significantly higher than that in the model group (P<0.05). Conclusions Noise exposure may induce dyslipidemias in rats. Zinc gluconate and Danshen injection can regulate the plasma lipid level following exposure to noise in rats, and combination of zinc gluconate and Danshen injection may be superior to zinc gluconate or Danshen injection alone.

Atrophy ; Brain ; pathology ; Child ; Female ; Humans ; Muscle Fibers, Slow-Twitch ; pathology ; Neuromuscular Diseases ; congenital ; pathology

Objective To understand a cancer hospital inpatient care Ningxia quality of service perceived status quo.Methods Servqual model-based,self-designed Expect Inpatient Nursing Service Quality Perception Questionnaire was used to investigate 160 hospitalized patients and expected to build expectations oncology inpatients quality of care and services-dimensional perception matrix (IPA matrix).Results By analyzing sensible quality (SQ) value and IPA matrix of the Servqual questionnaire,all items except for treating equally and recommend the hospital showed SQ＜0,that was,the actual feelings of patients were less than expected,and there was statistical difference between the actual feelings and expected feelings in the quiet ward,health guide,care level (t=2.963,2.020,2.020,P ＜0.05).IPA matrix showed that ward quiet,health guidance,care level was the priority problems for the hospital.Life Care,protection of privacy,psychological care,level of service,pay the money reached care were the minor priority problems.Conclusions Servqual Model and IPA matrix combination can help to understand the gap between expectations and perception of nursing service,identify problems improvement priorities,identify weaknesses in care for hospital managers,and develop fine effective theory.