Dental Pulp Cavity ; diagnostic imaging ; pathology ; Humans ; Radiography ; Root Canal Therapy

The temperature and salinity ranges for growth of twelve strains of marine Bdellovibrio-and-like organisms (BALOs) which affiliated with four different phylogenetic clusters in the family Bacteriovoracaceae and the lytic ability of them to six kinds of common shrimp pathogen vibrios were studied. The morphological characteristics of four representative strains were examined with transmission electron microscope. The results showed that the strains within the same cluster or sub-cluster had identical growth temperature or salinity. The growth temperature ranges of clusters IV, VI, IX and X strains were 20?C～35?C, 15?C～35?C, 15?C～40?C, 10?C～40?C, respectively, and the optimum growth temperature was about 30?C or 35?C. The growth salinity ranges of clusters IV, VI, IX and X strains were 5‰～40‰, 2.5‰～30‰, 5‰～60‰ and 5‰～60‰, and the optimum salinities were about 10‰, 5‰, 20‰ and 20‰, respectively. Three of the six kinds of vibrios tested were lysed by all the BALOs, while the others could only be lysed by some of the BALOs. However, differential lysis to some of the vibrios was also observed among BALOs within the same cluster. The four strains of different clusters all exhibited typical BALOs morphology, hav-ing vibrioid cell with a single polar flagellum.

Objective To observe the characterization of fat emboli in cardiac chamber in pig model and the patients undergoing total hip arthroplasty(THA) by transesophageal echocardiography(TEE). Methods Different dose of fatty liquid was injected slowly via the internal jugular vein. The changes of the image were observed by continuous TEE. The lethal dose of fat embolism was recorded. Twenty-two patients underwent TEE during THA. Gray scale and echo intensity of fat embolism in right atrium were studied quantitatively in varied periods of the operation. Results With the increase of fat liquid injected in pig model, the strong- echo particles in right atrium and ventricle became more and more, the imaging of the particles was from “moving star”, “shower-like” to “snow fluffy”, at last paradoxical fat embolism occurred when the dose of fat liquid was over 4 ml. The accumulated lethal dose of fat embolism was 15.8 ～ 27.8 ml.②Fat emboli appeared as strong- echo particles in right atrium were found in different period of THA. Average gray scale and echo intensity of emboli in right atrium were significantly higher in period B than in other periods during THA. Conclusions Fat emboli in cardiac chamber can be found sensitively by TEE. Intra-operative monitoring and quantitative analysis is helpful to identifying fat embolism syndrome.Paradoxical embolism is a reliable evidence of fat embolism syndrome.

Objective: To study the chemical constituents from the leaves of Rhodomyrtus tomentosa and its antibacterial activities. Methods: The compounds were isolated and purified by various column chromatographies. Their structures were elucidated by means of spectral analyses (MS and NMR). The antibacterial activity of the compounds was tested by micro dilution method Results: A total of 21 compounds were isolated from the 95% ethanol extract in the leaves of R. tomentosa and identified as (2R,4'R,8'R)-α-tocopherol (1), (2R,4'R,8'R)-β-tocopherol (2), α-tocopherol-quinone (3), α-tocopherol A (4), (-)-a-tocospirone (5), rhodomyrtosone F (6), rhodomyrtosone C (7), watsonianone A (8), rhodomyrtone (9), verimol K (10), methyl cinnamate (11), naringenin (12), quercetin (13), myricetin (14), 3,7,3'-trimethoxy-5,4',5'-trihydroxy flavone (15), 5,7,3',5'- tetrahydroxyflavanone (16), blumeatin (17), dihydroquercetin-7,4'-dimethylether (18), dihydroquercetin-4'-dimethoxy (19), 2,4,7,8,9,10-hexahydroxy-3-methoxyanthracene-6-O-β-L-rhamnopyranoside (20), and 4,8,9,10-tetrahydroxy-2,3,7-trimethoxyanthracene-6-O-β-D-glucopyranoside (21). Conclusion: Compound 6 is a new natural product and compounds 2-5, 10-13, and 15-19 are isolated from the plants of Rhodomyrtus (DC.) Reich for the first time. Compounds 3 and 6-9 exhibit the antibacterial activities.

ObjectiveTo analyze the characteristics of top of the basilar syndrome (TOBS) in clinic and imaging.MethodsData of 31 TOBS cases were analyzed retrospectively.ResultsThe clinical features of TOBS patients were sudden unconsciousness or vertigo and dyskinesia of the limbs, the dismovement of the eyeballs, abnormality of the pupils, partial blindness or cortical blindness, hypesthesia, disturbance of memory and counting. CT and MRI showed multi-infarction included thalami, occipital lobe, cerebellum, midbrain, temporal lobe.ConclusionTOBS can be diagnosed accurately according to clinical features and imaging signs.

Animals ; Comet Assay ; DNA Damage ; drug effects ; Lymphocytes ; drug effects ; Male ; Organometallic Compounds ; toxicity ; Rats

The research of sleep staging is an important basis of evaluating sleep quality and diagnosing diseases. In order to achieve automatic sleep staging, we proposed a new method which combines with principal component analysis (PCA) and support vector machine (SVM) for automatic sleep staging. Firstly, we used PCA to reduce dimension of time-frequency-space domains and nonlinear dynamical characteristics of sleep EEG from 5 subjects to reduce data redundancy. Secondly, we used 1-a-1 SVM to classify sleep stages. The results showed that the correct rate can reach 89.9%, which was better than those of many other similar studies.
Electroencephalography ; Humans ; Nonlinear Dynamics ; Principal Component Analysis ; Sleep Stages ; Support Vector Machine

We applied Lempel-Ziv complexity (LZC) combined with brain electrical activity mapping (BEAM) to study the change of alertness under sleep deprivation in our research. Ten subjects were involved in 36 hours sleep deprivation (SD), during which spontaneous electroencephalogram (EEG) experiments and auditory evoked EEG experiments-Oddball were recorded once every 6 hours. Spontaneous and evoked EEG data were calculated and BEAMs were structured. Results showed that during the 36 hours of SD, alertness could be divided into three stages, i. e. the first 12 hours as the high stage, the middle 12 hours as the rapid decline stage and the last 12 hours as the low stage. During the period SD, LZC of Spontaneous EEG decreased over the whole brain to some extent, but remained consistent with the subjective scales. By BEAMs of event related potential, LZC on frontal cortex decreased, but kept consistent with the behavioral responses. Therefore, LZC can be effective to reflect the change of brain alertness. At the same time LZC could be used as a practical index to monitor real-time alertness because of its simple computation and fast calculation.
Attention ; physiology ; Brain Mapping ; Electroencephalography ; Evoked Potentials ; Humans ; Nonlinear Dynamics ; Sleep Deprivation

Rupture of unstable atherosclerotic plaque is an essential pathogenetic mechanism of acute coronary syndrome (ACS), thereby, to stabilize the vulnerable plaque is of great importance for prevention and treatment of ACS. Recent study has shown the multi-target effects of traditional Chiese medicine intervention in stabilizing unstable atherosclerotic plaque is promising. The literatures involving this topic in recent years were reviewed in this paper.
Acute Coronary Syndrome ; complications ; drug therapy ; pathology ; Animals ; Coronary Artery Disease ; complications ; drug therapy ; pathology ; Coronary Vessels ; pathology ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Phytotherapy

Objective To evaluate pigtail catheter mashing thrombosis combined with catheter directed thrombolysis in the treatment of acute iliofemoral venous thrombosis complicated by Cockett syndrome in the left lower limbs.Method Data of 137 cases of acute iliofemoral venous thrombosis complicated with Cockett syndrome in left lower limb by interventional therapy from January 2007 to October 2012 were analyzed retrospectively.Inferior vena cava filters were placed in all of the patients.Patients were divided into two groups:Group A (n =81) treated with catheter directed thrombolysis only,Group B (n =56) treated with pigtail catheter mashing thrombosis combined with catheter directed thrombolysis.After operation,patients were treated by anticoagulation with urokinase and heparin calcium,and then warfarin for 6 to 1 2 months.Results The thrombolysis time in group B was significantly shorter than that in group A (P ＜0.01),the dosage of urokinase was significantly less than that in group A(P ＜ 0.01).The venous patency score in group B after therapy was significantly better than in group A (P ＜ 0.01).121 patients were followed up for 10-60 months.There were no pulmonary embolism.Conclusions Pigtail catheter mashing thrombosis combined with catheter directed thrombolysis in the treatment of acute iliofemoral venous thrombosis complicated with Cockett syndrome in left lower limb can improve thrombolytic efficiency,shorten thrombolysis time,reduce the use of urokinase.