1.Endothelial dysfunction and lipid oxidation status after coronary artery bypass grafting procedure under cardio-pulmonary bypass
Zhenxiao JIN ; Yanping LI ; Mei XIN ; Weibin XUE ; Qin CUI
Medical Journal of Chinese People's Liberation Army 2006;31(1):15-17
Objectives To evaluate the influence of cardio-pulmonary bypass (CPB) to the endothelial function and lipid oxidative status of the Coronary Artery Bypass Grafting (CABG) patients. Methods Thirty-five coronary patients (female 5 cases, male 30 cases, mean age 59.5±8. 6 years) who needed CABG operation therapy were included. The control group consisted of 20 health volunteers (female 5 cases, male 15 cases, mean age 49. 5 ± 7.3 years).Blood samples were taken at preoperative, 3 and 7 days postoperative. Serum von Willebrand factor (vWF) level, one of the endothelial dysfunction markers,and oxidized low density lipid (OxLDL) level, one of the lipid oxidization status markers were detected with ELISA kits. Results The levels of vWF and OxLDL in CABG patients' serum were elevated significantly compared with those of control group ( 139.60% ± 34. 39% vs 82. 79% ±13. 89% and 63. 7 ±18. 9U/ml vs 25.4± 15.2U/ml respectively). Three and 7 days after CABG significant increases in the vWF level (194. 30% ±37. 74%; 181.07% ±31.05%vs 139.60% ±34.39% at baseline, P<0. 0001, respectively) and OxLDL level (79. 3±26. 7U/ml; 72.4±23.1U/ml vs 63.7±18. 9U/ml at baseline, P<0. 01, respectively) were detected. Conclusion Compared with normal people, the endothelial system of CABG patients is impaired, and their lipids are in a more oxidative status. CABG procedure with CPB can further deteriorate the conditions, which may imply that the patients are predisposed to acute coronary accident shortly are the procedure.
2.Simultaneous determination of four anthraquinones in rat plasma by HPLC-FLD method and its pharmacokinetic study.
Jin-Qiu HUANG ; Xue-Mei YAN ; Fang FENG
China Journal of Chinese Materia Medica 2014;39(19):3850-3854
This paper aims to develop a method for the determination of aloe-emodin, rhein, chrysophanol and physcion and study the pharmacokinetic properties of four anthraquinones in rat plasma after oral administration of gardenia and rhubarb decoction. The plasma concentrations at different time points of four anthraquinones were determined by HPLC-FLD method. Plasma samples were extracted with liquid-liquid extraction procedure. Plasma samples were separated on a C18 column (4.6 mm x 150 mm, 5 μm), using 0.2% acetic acid and methanol as mobile phase at a flow rate of 1.0 mL min(-1) with gradient elution. The excitation and emission wavelengths were set at 430, 525 nm, respectively. DAS 2.0 software was applied to calculate the pharmacokinetic parameters. The results showed four anthraquinones can be absorbed. The main parameters of aloe-emodin, rhein, chrysophanol and physcion were as follows: C(max) for aloe-emodin was (0.085 ± 0.058), (3.772 ± 1.152), (0.464 ± 0.267), (0.028 ± 0.008) mg x L(-1) respectively; t(max) for rhein was (1.042 ± 0.510), (0.805 ± 0.307), (1.167 ± 0.283), (0.616 ± 0.162) h respectively; t½ for chrysophanol was (3.557 ± 1.250), (6.879 ± 1.126), (5.196 ± 2.032), (4.337 ± 1.816) h; AUC(0-t) for physcion was (0.504 ± 0.130), (9.558 ± 1.106), (2.545 ± 1.554), (0.052 ± 0.018) mg x h x L(-1). This paper developed a selective, accurate and sensitive HPLC-FLD method for the simultaneous determination of four anthraquiones in rat plasma.
Animals
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Anthraquinones
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blood
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pharmacokinetics
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Chromatography, High Pressure Liquid
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instrumentation
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methods
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Drugs, Chinese Herbal
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analysis
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
3.Relationship between autophagy and T2DM and intervention effect of traditional Chinese medicine.
Jin-ni HONG ; Wei-wei LI ; Xue-mei WANG
China Journal of Chinese Materia Medica 2015;40(22):4351-4354
Insulin resistance and insulin secretion deficiency are main machanisms in inducing type 2 diabetes mellitus (T2DM), and mitochondria damage plays an important role in them. Research shows that autophagy is a self-protective mechanism of cells, which plays an important role in maintaining the normal structure and function of pancreatic β cells and improving insulin resistance. Previous studies show that traditional Chinese medicine can regulate cell autophagy to influence β cells and insulin resistance, type 2 diabetes mellitus and its complications. Thus this review will talk about the process of the relationship between autophagy and T2DM and the intervention effect of traditional Chinese medicine.
Animals
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Autophagy
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drug effects
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Diabetes Mellitus, Type 2
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drug therapy
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metabolism
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physiopathology
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Insulin
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metabolism
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Insulin Resistance
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Insulin-Secreting Cells
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cytology
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drug effects
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metabolism
5.Effects of cervical mild hypothermia auxillary therapy on stress reaction in patients with cervical spinal cord injury
Xue-Mei PAN ; Hai-Zhang DENG ; Xue-Mei ZHANG ; Li-Ju JIN
Chinese Journal of Modern Nursing 2009;15(13):1201-1204
Objective To investigate the effcts of cervical mild hypothermia auxiliary therapy on stress reaction in patients with cervical spinal cord injury. Methods 84 cases cervical spinal cord injury patients were randomly divided into two groups:a cervical mild hypothermia group (42 cases)and a routine treatment group (42 cases), 64 healthy men who come for health examination were recruited and served as healthy control. Routine treatment were used in both treatment groups, in edition, the LM-HAT was just applied in mild hypothermia group. The changes of the concentrations of corticotropin releasing hormone (CRH), adrenocorticotropin hormone (ACTH), corticosteroid (Cor), arginine vasopessin (AVP) in serum and the symptoms of the stress dsease(hypertension, high blood sugar)were observed before treatment and 1 week after treatment. Results The concentrations of CRH, ACTH, Cor, AVP in serum and the symptoms of stress disease in mild hypothermia group and routine treatment group were improved significantly at week after treatment(P<0.05), but the therapeutic effects of mild hypothermia group were better than that of routine treatment group (P<0.05). Conclusions The cervical mild hypothermia auxiliary therapy could significantly inhibit the secretion of stress hormones, make the H-P-A axis stable and promote the stress disease recovery.
7.The innovation of cultivation system of stomatological talents
Ling-lin ZHANG ; Jin-mei RAN ; Ling YE ; Qian LIU ; Xue-dong ZHOU
Chinese Journal of Medical Education Research 2011;10(11):1305-1308
The rapid development of stomatology is improving the standard of talent quality and skills gradually,so the innovation of cultivation patterns of the stomatology students is imperative.West China College of Stomatology in Sichuan University is practicing the innovation of cultivation system of stomatological talents by establishing the new teaching and learning plan,adjusting the course system,strengthening the teaching materials construction,and adjusting the evaluation index and so on.The goal of the innovation of cultivation patterns is to foster the stomatological talents which have profound cultural atmosphere,the solid professional knowledge,strong innovative consciousness,and broad international vision.
8.Some Teaching Experience by Using the Mode of Learning Followed by Teaching
Sheng-Ping XUE ; Hui-Li LI ; Jin-Liang ZHANG ; Xiang-Mei ZHANG ; Na CUI ;
Microbiology 2008;0(11):-
A new teaching method was developed in the curriculum of Harmful Microorganisms Control Technology.It is characterized by students’ self-learning followed by student’s instruction.Both students and teacher have succeeded in this model after four stages of practice,in which a pleasant learning atmosphere was created in the classroom.An effective interaction between teacher and students was achieved.Students are viewed as main objects in the classroom and they are encouraged to ask questions,to formulate their own ideas,or to find things out for themselves.Thus,students’ abilities including presentation,communication,competition,and cooperation were enhanced.By adapting their role to the new teaching method,teachers have also improved their teaching skill and strategies.
9.Study on severe acute respiratory syndrome seroepidemiology after its outbreak in a general hospital
Zhen YANG ; Hai-Ke DU ; Bin SU ; Ying JIN ; Xue-Mei DONG ; Shi-Xin WANG ;
Chinese Journal of Infectious Diseases 2000;0(02):-
Objectives To study the level and development of serum specific antibody against severe acute respiratory syndrome coronavirus(SARS-CoV)of different populations in SARS pestilence district after SARS outbreak in a general hospital.Discuss SARS sub-clinical infection and protective action of the IgG antibody.Methods Seroepidemiology method,enzyme-linked immunosorbent assay (ELISA)and indirect immunfluorescence assay(IFA)were employed to investigate the changing level of serum antibody to SARS-associated coronavirus in non-SARS population in SARS pestilence district during and after SARS outbreak.The development of IgM and IgG antibody in patients with SARS in 6 weeks after the onset of SARS was studied qualitatively.The level changing of IgG antibody in con- valescent patients with SARS in 82 weeks after the onset was observed dynamically.Results The ELISA test outcome of IgG antibody was negative in 200 non-SARS people who were random samples of normal mass in SARS pestilence district and common community.The positive rate was 0.41% in 487 SARS high risk population tested by ELISA,but showed negative when retested by IFA.The A value level of IgG antibody existed significant difference in non SARS mass during and after SARS outbreak and the later's was higher them the former's(P
10.Clinical research on the treatment of dacryocystitis combined with canalicular obstruction by dacryocystorhinostomy with canalicular intubation
Xin-Jun, XUE ; Xiao-Jun, ZHANG ; Jin, LIU ; Su-Mei, LI
International Eye Science 2014;(7):1340-1341
AlM: To investigate the clinical efficacy of treatment of dacryocystitis combined with canalicular obstruction by endonasal endoscopic dacryocystorhinostomy with canalicular intubation.
METHODS: A total of 27 cases of dacryocystitis combined with canalicular obstruction were treated by endonasal endoscopic dacryocystorhinostomy with canalicular intubation. Canalicular obstruction was treated by laser under dacryoendoscopy and antegrade intubation.
RESULTS: For 27 cases, operations were successful, with no complications. All patients were followed up for 6mo, 25 were cured, 2 were effective with no failed. The cure rate was 93%.
CONCLUSlON:Treatment of dacryocystitis combined with canalicular obstruction by endonasal endoscopic dacryocystorhinostomy with canalicular intubation has clear field, minimal invasion, quick recovery, exact effect and less recurrence, so it is worthy of promotion.