2.Construction of underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2.
Xue-lin HAN ; Xue-mei CHEN ; Fei ZOU
Chinese Journal of Applied Physiology 2008;24(4):508-512
AIMTo construct underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2.
METHODSThe combined plasimid pSilencerHSP90alpha and pSmycHSP90beta were introduced into HepG2 by electroporation, respectively. The result of transfection was identified by Western-blotting and the curve of cell growth was drew by MTT. Observe the cell vitality and expression of HSP90.
RESULTSExpression of HSP90 in transfected cell line was shown by Western-blotting: Compared with control, expression of HSP90 in the cells transfected with pSilencerHSP90alpha decreased, whereas that in the cells transfected with pSmycHSP90beta increased.The growth curves of the two groups of transfected cells was as the same as that of the control group.
CONCLUSIONThe stable overexpression HSP90beta and underexpression HSP90alpha HepG2 cell lines were established.
Base Sequence ; Electroporation ; HSP90 Heat-Shock Proteins ; genetics ; metabolism ; Hep G2 Cells ; metabolism ; Humans ; Molecular Sequence Data ; RNA, Messenger ; genetics ; metabolism ; RNA, Small Interfering ; genetics ; Transfection
3.Complete Genomic Sequence Analysis of a New SV40 Isolate
Xue-mei, ZHANG ; Yan-chun, CHE ; Jing-jing, WANG ; Long-ding, LIU ; Ming-xue, XIE ; Qi-han, LI
Virologica Sinica 2007;22(1):41-45
The genome of a new SV40 strain(SV-IMB)isolated from a rhesus monkey was completely sequenced and compared with other isolates.The results showed that the whole genome contains 5246bp,and the average identity of SV-IMB was 98.1% as compared to other SV40 isolates.Its regulatory region is composed of a complete enhancer and a defective enhancer.Amino acid changes occurred to some extent in both the large T antigen (T-Ag) and VP1 region.The findings demonstrate that the SV-IMB is a new SV40 isolate.
4.Study on Measuring Cord Blood Leptin Level and the Ralatioship between Leptin and Insulin in Premature Infants
xue-ai, FAN ; yan-min, LI ; xiao-mei, HAN ; zhao-xia, TIAN
Journal of Applied Clinical Pediatrics 1986;0(02):-
0.05).2.Neonates umbilical serum leptin concentration was positively correlated with body mass index(r=0.520 P
5.The mechanism of rosiglitazone compound based on network pharmacology.
Yu BAI ; Xue-mei FAN ; Han SUN ; Yi-ming WANG ; Qiong-lin LIANG ; Guo-an LUO
Acta Pharmaceutica Sinica 2015;50(3):284-290
Applications of network pharmacology are increasingly widespread and methods abound in the field of drug development and pharmacological research. In this study, we choose rosiglitazone compound as the object to predict the targets and to discuss the mechanism based on three kinds of prediction methods of network pharmacology. Comparison of the prediction result has identified that the three kinds of prediction methods had their own characteristics: targets and pathways predicted were not in accordance with each other. However, the calcium signaling pathway could be predicted in the three kinds of methods, which associated with diabetes and cognitive impairment caused by diabetes by bioinformatics analysis. The above conclusion indicates that the calcium signaling pathway is important in signal pathway regulation of rosiglitazone compound, which provides a clue to further explain the mechanism of the compound and also provides a reference for the selection and application of methods of network pharmacology in the actual research.
Calcium Signaling
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Cognitive Dysfunction
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Computational Biology
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Diabetes Mellitus
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Humans
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Pharmacology
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methods
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Thiazolidinediones
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pharmacology
6.Effect of environmental factors on photosynthetic physiology and flavonoid constituent of Scutellaria baicalensis.
Yong-Gang ZHANG ; Mei HAN ; Xue JIANG ; Sheng-Nan ZHAO ; Li-Min YANG
China Journal of Chinese Materia Medica 2014;39(10):1761-1766
To discuss the effect of environmental factors and photosynthesis on the growing of plant and the content of active components in Scutellaria baicalensis, the photosynthetic physiology index and diurnal changes of flavonoid constituent of S. Baicalensis were observed and tested in flowering and fruiting stages, and in the meantime environmental parameters were recorded. The obtained data were analyzed data by using path analysis and gray correlation analysis. The results showed that PAR and SWC were important environmental factors impacting on photosynthesis of S. baicalensis. SWC, RH and Ca were important environmental factors impacting on baicalin content. PAR, Po and Ta were important environmental factors impacting on baicalein content.
Environment
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Flavonoids
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analysis
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metabolism
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Photosynthesis
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Plant Extracts
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analysis
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metabolism
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Plant Roots
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chemistry
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growth & development
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metabolism
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Scutellaria baicalensis
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chemistry
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growth & development
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metabolism
7.Primary bone lymphom:report of 7 cases and literature review
Mei XUE ; Jing LIU ; Ling ZHU ; Xiaoli ZHENG ; Li DING ; Dongmei HAN ; Hengxiang WANG
Practical Oncology Journal 2016;30(1):41-43
To explore the clinic characters,treatments and prognosis of patients with primary bone lym-phoma( PLB ) .The clinical symptoms, signs, X -ray features, pathological morphology, immunophenotype and treatment of 7 patients with PLB were analyzed retrospectively and the pertinent literatures were reviewed.The re-sults showed that the main complains of 7 cases of PBL were local pain.The CT showed osteolytic bone destruc-tion and soft tissue swelling.There were 3 cases of diffuse large B cell lymphoma,1 case of Burkitt-type lympho-ma,1 case of periferal T-cell lymphoma,1 case of anaplastic large cell lymphoma,and 1 case of Hodgkins lym-phoma.2 patients presented with stageⅠ,4 with stageⅡ,and 1 with stage 3.The therapeutic procedure includes local radiotherapy combined with chemotherapy and targeted therapy.The clinical presentation of PLB is not spe-cial.The diagnosis and identification of histological type of PLB should be established by histopathological and im-munohistochemistry examinations.Early diagnosis and active therapy could improve the prognosis of PLB.Combi-nation therapy including radiotherapy and chemotherapy is the optimal treatment for PLB.
8.Influence of statins on antiplatelet effects of clopidogrel
Hongsong ZHANG ; Mingyuan LIU ; Mei HAN ; Feng CHEN ; Jincheng ZHAO ; Xue BAI ; Yu YANG ; Guangyuan YANG
Chinese Journal of cardiovascular Rehabilitation Medicine 2015;24(5):543-546
Objective:To observe influence of statins on antiplatelet activity of clopidogrel and provide basis for ra‐tionality of statins combined clopidogrel treatment .Methods :According to random number table ,a total of 90 pa‐tients diagnosed as acute coronary syndrome were equally divided into clopidogrel group ,clopidogrel + simvastatin group and clopidogrel + pravastatin group . Three groups received corresponding routine medication treatment . Plasma levels of platelet αgranule membrane protein (CD62P) ,lysosomal granule membrane glycoprotein (CD63) and maximum platelet aggregation rate (MPAR) were measured and compared among three groups before and 3d af‐ter treatment .Results:Compared with before treatment ,after treatment ,there were significant reductions in plas‐ma levels of CD62P and CD63 and MPAR in three groups , P<0.01 all .After treatment ,there were no significant difference in plasma levels of CD62P [ (14.63 ± 3.45) ng/ml vs .(14.14 ± 4.32) ng/ml vs .(14.59 ± 4.23) ng/ml] , CD63 [ (26.32 ± 10.43) ng/ml vs .(27.04 ± 10.75) ng/ml vs .(27.29 ± 9.27) ng/ml] and MPAR [ (28.62 ± 17.68)% vs .(28.38 ± 16.43)% vs .(29.13 ± 14.23)% ] among clopidogrel group ,clopidogrel + simvastatin group and clopidogrel + pravastatin group ,P>0.05 all .Conclusion:Short‐term and routine dose of statins combined clo‐pidogrel is feasible in treatment of acute coronary syndrome .The combined use of them will not affect antiplatelet function of clopidogrel .
9.The Interaction Between Human TRIM5? Chimera and HIV-1gag Protein in Vitro
Xiang-Ping MENG ; Xiu-Ying LI ; Han-Xiao SUN ; Xue-Mei MO ;
China Biotechnology 2006;0(05):-
Objective:To express and purify the TRIM5? chimaera[TRIM5? H(R328-332)] protein and to explore the interaction between the TRIM5? H(R328-332)and HIV-1gag. Methods:The plasmid pET28aTRIM5? H(R328-332) was transformed to E.coli BL21 (DE3) strain ,and the expression of TRIM5? H(R328-332) protein was induced by IPTG,purified with Ni2+ chromatography.The expression and purification of TRIM5? H(R328-332) were analyzed by SDS-PAGE and Western blot,and the interaction between TRIM5? H(R328-332) and HIV-1gag was detected by co-immunoprecipitation,His pull-down and ELISA. Results:The recombinant plasmid pET28aTRIM5? H(R328-332) was successfully expressed in E.coli. The results showed that the purified full length TRIM5? H(R328-332) interacted with HIV-1gag protein. Conclusion:The human TRIM5? chimaera was expressed successfully in vitro,and the study demonstrates that the human TRIM5? chimaera interacts with HIV-1 gag in vitro.
10. Advances in the Function of Cytochrome P450 Enzymes in Brain and Their Research Progress in Neuropsychiatric Disease
Chinese Pharmaceutical Journal 2020;55(10):789-793
Cytochrome P450 enzymes participate in the biotransformation of various organic compounds in living organisms. Cytochrome P450 enzymes are present and active in various brain regions, which mediate the synthesis and metabolism of various endogenous substances in the brain, so the enzymes play an important role in maintaining homeostasis of the central nervous system. And cytochrome P450 enzymes in brain are widely involved in metabolism of central drugs such as tramadol, so the changes of their activities or expressions may affect the drug's efficacy in brain. In addition, in view of the important roles of cytochrome P450 enzymes in the biotransformation of endogenous and exogenous substances in brain, the importance of their genetic polymorphism or activity changes in neuropsychiatric diseases is being gradually described. Therefore, in order to provide theoretical basis for the development of related targeted therapeutic drugs and explore whether cytochrome P450 enzymes in the brain can be used as therapeutic targets for central diseases, the development in the function of cytochrome P450 enzymes in brain and their recent advances in neuropsychiatric diseases by reviewing the relevant research in recent years are summarized.