Recently,considerable progress has been made in the treatment of systemic lupus erythematosus(SLE),especially in the clinical use of new immunosuppressive agents,which leads to improved remission rate and life quality.However,plasmapheresis still shows to be necessary in severe lupus with serious complications.With the development of plasmapheresis technology,the efficiency and specificity of antibody adsorption being improved significantly,it played an important role in the treatment of acute stage life-threaten lupus.Here we intend to make a review on the relative improvement of plasmapheresis technology and its clinical use.

ObjectiveTo evaluate 125I-labeled monoclonal antibody(McAb) CL58 in radioimmunoguided surgery(RIGS) for patients with colorectal cancer(CC).Methods 125I-McAb-CL58 was injected submucously into pericancerous site under endoscope in 29 CC patients. Intraoperative radioimmunodetection on targets(T) of tumors, regional lymph nodes and incisional margins, was carried out using a portable gamma-detecting probe. The T/NT ratio of 3∶1 was taken as the lowest threshold value of positivity for primary lesions, wall infiltration and lymph node metastasis. Lymph nodes were stained immunohistochemically for micrometastasis. Results The sensitivity for primary lesions was 93 1%. The specificity for tumor margin was 95 5%. For lymphatic metastasis, the sensitivity of RIGS was 92 0%, and the specificity was 87 8%(all P

Animals ; Brain-Derived Neurotrophic Factor ; genetics ; Cells, Cultured ; Female ; Gene Expression ; drug effects ; Neurons ; drug effects ; Phenylalanine ; toxicity ; Phenylketonurias ; complications ; Rats ; Rats, Sprague-Dawley ; rho GTP-Binding Proteins ; genetics

Genetic Diseases, Inborn ; economics ; prevention & control ; therapy ; Humans ; Metabolic Diseases ; economics ; prevention & control ; therapy

Objective To explore the effect of the thyroid morphologic change,the initiating treatment time and the thyroid function(serum FT_4,TSH)on mental development in children with congenital hypothyroidism (CH).Methods After diagnosed,52 cases of CH were orally administrated with L-T_4,then their mental and physical development were monitored and thyroid morphologic changes were observed by thyroid imaging(~(99m)TcO_4) and type B ultrasonography.The Bayley test was performed at the age of 10 to 30 months and expressed as mental developmental index(MDI)and psychomotor developmental index(PDI).Analysis was conducted upon treatment timing and thyroid morphologic changes.Results The MDI in those cases with morphologic abnormalities treated within 90 days was significantly lower than that with normal morphology(91?20 vs 105?15,P

Objective To determine the optimum cuff pressure of the flexible laryngeal mask airway (LMA) for airway management in pediatric patients.Methods One hundred and twenty pediatric patients undergoing strabismus surgery with general anesthesia,of American Society of Anesthesiologists physical status Ⅰ or Ⅰ,aged 3-10 yr,were randomly divided into 3 groups (n=40 each) according to the cuff pressure of the flexible LMA:20 cmH2O pressure group (group A),40 cmH2O pressure group (group B) and 60 cmH2O pressure group (group C).The cuff was inflated to the predetermined pressure using the inflatable cuff manometer in each group.Oropharyngeal leak pressure was measured after LMA placement.Peak airway pressure and the difference between inhaled and exhaled tidal volume were recorded at 5 min of positive pressure ventilation.The LMA insertion condition,LMA removal time,and development of sore throat,hoarseness,dysphagia and abdominal distention within 24 h after operation were recorded.Results There were no significant differences in the success rate of LMA placement at first attempt,peak airway pressure,or incidence of sore throat among the three groups (P＞0.05),and no hoarseness or dysphagia was found in the three groups.Compared with group A,oropharyngeal leak pressure was significantly increased,and the difference between inhaled and exhaled tidal volume and incidence of abdominal distention were decreased in B and C groups (P＜0.05).There was no significant difference in the parameters mentioned above between group B and group C (P＞0.05).Conclusion The optimum cuff pressure of the flexible LMA is 40 cmH2O when used for airway management in the pediatric patients.

Objective: To learn the epidemiological characteristics of infectious disease related public health emergencies in Zhejiang Province from 2010 to 2018 for the prevention and control. Methods: The surveillance data was extracted from National Public Health Emergency Management Information System. Descriptive epidemiology method was used to analyze main diseases as well as distribution characteristics of time and places. Results: A total of 445 events were reported, which caused 14362 cases and 34 deaths, with a attack rate of 0.69% and mortality rate of 0.24%. There were 298 events with less than 30 cases, accounting for 66.97%. The event classification was dominated by general events ( 242 events, 54.38% ) and ungraded events ( 201 events, 45.17% ). The main diseases were chickenpox ( 134 events, 30.11% ), hand foot mouth disease ( 59 events, 13.26% ) and other infectious diarrhea ( 51 events, 11.46% ). The incidence peaked from April to June ( 129 events, 28.99% ) and from November to December ( 131 events, 29.44% ). Ningbo ranked the top in the number of reported events ( 141 events, 31.69% ). Most events ( 322 events, 72.36% ) occurred in schools. Conclusions The infectious disease related public health emergencies in Zhejiang Province from 2010 to 2018 were mainly caused by chickenpox, hand-foot-mouth disease and other infectious diarrhea. The two peaks of the emergencies occurred from April to June and from November to December. Ningbo was the main area reporting infectious diseases, and schools were the main places.

ObjectiveTo explore effect of interleukin-10 (IL-10) on histopathologic scores of severe acute pancreatitis (SAP) and the level of serum amylase (AMY) in rats.Methods48 female and male adult Sprague Dawley rats (200—300g) were randomly allocated into three groups: OPgroup, SAP group and IL-10 group with 16 rats in each group. SAP was made with retrograde ductal infusion of 5% sodium taurocholate solution.ResultsIn SAP and IL-10 group, there were interstitial edema, necrosis, neutrophil infiltration and interstitial hemorrhage of pancreas, more or less. At 6h and 12h after models were made, the pancreatic histopathologic score in IL-10 group (4.00±0.33 and 6.25±0.25) were significantly lower than that in SAP group (6.13±0.35 and 9.50±0.50)(P<0.01). At 6 h after models were made, the serum AMY in IL-10 group was lower than that in SAP group (P<0.05), but at 12 h there were no differences.ConclusionIn earlier period of SAP in rats, IL-10 can lower the serum AMY level, and significantly reduced pancreatic histologic score (edema, inflammation, hemorrhage, and necrosis).

Objective To investigate the removal effect of immunoadsorption (IA) on associated antibodies and the efficacy in late-onset myasthenia gravis (MG). Methods A total of 25 late-onset MG patients were randomly selected to enroll in this study. IA therapy was given to 10 patients (IA group), while immunoglobin (0.4 g·kg-1·d-1) was administrated to the other 15 patients for 5 days(Ig group). The titers of Titin antibody (Titin-ab), acetylcholine receptor antibody (AchR-ab) and presynaptic membrane antibody (PrsmR-ab) were detected before and after the treatment. Quantitive MG (QMG) score was assessed before and immediately after the entire course of treatment. The clinical efficacy, the duration of respiratory support and in-hospital were compared between two groups. The correlation between three antibodies and QMG score was also analyzed. Results Compared with that before treatment, the Titin-ab PIN values, the AchR-ab PIN values, and the PrsmR-ab P/N values of IA group were all decreased significantly after treatment (P<0.05, respectively). The P/N value of Titin-ab in IA group was decreased by 54.7%～3.5%, which was significantly higher than that in Ig group(19.9%±3.1%) (P<0.01). QMG score reduced by 42.4%± 4.2% and 23.8%±3.7% in IA group and Ig group respectively (P<0.01, respectively). Symptoms were effectively ameliorated by both treatments, but the effective power of IA group was higher than that of Ig group (70% vs 40%, P<0.05). Remission time of IA group was significantly shorter than that of Ig group [(5.38±0.42) d vs (8.4±1.54) d, P=0.008), so was the duration of in-hospital [(13.50±0.50) d vs (16.50±0.50) d, P<0.05). The number of respiratory support in IA group was less than that in Ig group (1/10 vs 6/15, P<0.05). By the Pearson correlation analysis, the decrease of Titin-ab showed a better longitudinal correlation with the decrease of QMG score than the other two antibodies (r=0.6315, P<0.01). Conclusion IA can rapidly and effectively clear the pathogenic antibodies of late-onset MG patients and its short-term clinical efficacy is better than immunoglobin.

Acquired Immunodeficiency Syndrome ; prevention & control ; China ; epidemiology ; Condoms ; utilization ; Humans ; Surveys and Questionnaires ; Transients and Migrants