1.Clinical application of INRatio coagulometer for fast measuring international normalized ratio
Yanping YIN ; Xinchun YANG ; Jing XUE
Chinese Journal of Postgraduates of Medicine 2012;35(24):14-17
ObjectiveTo compare the correlation and agreement of international normalized ratio (INR) measured by INRatio coagulometer and standard laboratory venous blood measurement in order to judge the clinical application value of measuring INR by INRatio coagulometer.MethodsOne hundred who received warfarin were chosen randomly.Venous blood and finger capillary blood were taken simultaneously for measurement of INR by laboratory coagulation analyzer SYSMEX CA-7000 and INRatio coagulometer respectively.The correlation and agreement of INR results tested by the two methods were analyzed.Results When INR ≤5,there was a good correlation between INRatio coagulometer and laboratory coagulation analyzer SYSMEX CA-7000 (r =0.898,P =0.000).The mean bias of the two methods was 0.36 and INR measured by INRatio coagulometer were higher than those tested by laboratory coagulation analyzer SYSMEX CA-7000.By Bland-Altman,it showed a good agreement for 91.2% (62/68) of the difference INR located 95% the limited of agreement when the mean INR 1.60 - 3.50,and a bad agreement for only 50.0%(1/2) of the difference INR located 95% the limited of agreement when the mean INR > 3.50.ConclusionsIn the therapeutic range,INR measured by INRatio coagulometer shows a good correlation and agreement with INR measured by the established laboratory method.INRatio coagulometer can be used to monitor INR in the patients received oral anticoagulant therapy.
2.Mechanisms of probiotic treatment of recurrent respiratory tract infections
Ruwen CHEN ; Jing YANG ; Hui XUE
Chinese Pediatric Emergency Medicine 2012;19(5):539-540
Recurrent respiratory tract infection (RRTI) is a common chronic respiratory disease of children.The pathogenesis is complex,etiology and treatment methods are varied.Probiotics are immune modulators with extensive clinical application.This paper reviewed the changes of intestinal microflora in children with recurrent respiratory tract infection and the mechanisms of probiotic treatment including intestinal flora regulation,intestinal mucosal barrier,stimulation of the growth of intestinal mucosa and immune regulation.
3.Kabuki syndrome case report.
Xue-jing YANG ; Yan ZENG ; Feng XIONG
Chinese Journal of Pediatrics 2010;48(11):868-870
4.Effects of Traditional Chinese Medicine Treatment from Lung on mRNA Expression of iNOS in Lung Tissues of Rats with Ulcerative Colitis
Xue YANG ; Xinyue WANG ; Shan JING ; Shu YANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(4):753-757
This study was aimed to observe effects of traditional Chinese medicine (TCM) treatment from lung on mRNA expressions of iNOS in lung tissues of rats with ulcerative colitis (UC). A total of 52 rats were used to estab-lish the UC rat model by using rabbit intestinal mucosal tissue allergenic model and TNBS-ethanol model (with the model success rate of 78%). Eight rats, which were randomly selected from 40 successfully modeled rats and the normal group, were used as the model group and the normal group before intervention (time point of week zero). The rest 32 successfully modeled rats were randomly divided into the model group, the western medicine (salazosulfapyri-dine) group, treatment from lung (Huang-Qi Jie-Geng, HQJG decoction) group, and treatment from intestine (Huang-Qi Huang-Lian, HQHL decoction) group, with 8 rats in each group. Another 8 normal rats were used as the control group. The intervention was given for 4 weeks. And the mRNA expression of iNOS was detected in week ze-ro, and four weeks after the treatment using real time-PCR. The results showed that in the acute phase of week zero, the mRNA level of iNOS in lung tissues of model group was significantly increased compared with that in normal group (P < 0.05); after 4-week treatment, compared with the normal group, the mRNA level of iNOS in the model group was significantly increased (P< 0.05). Compared with the model group, the mRNA level of iNOS in the treat-ment from lung group was significantly decreased (P < 0.01). It was concluded that NO which was catalyzed and synthesized by iNOS played an important role in pulmonary diseases. It can cause airway inflammation, edema, lung injury, and etc. Treatment from lung can alleviate the lung injury by inhibiting abnormal activation of iNOS.
5.Psychological flexibility and anxiety and depression in postoperative thyroid carcinoma patients
Yajin GUO ; Jing CAO ; Xue YANG ; Zhuohong ZHU
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(8):715-718
Objective To explore the relationship among psychological flexibility,anxiety and depression in postoperative thyroid carcinoma patients.Methods A total of 184 postoperative thyroid carcinoma patients selected randomly were measured with Acceptance and Action Questionnaire-Second Edition (AAQ-Ⅱ),Cognitive Fusion Questionnaire (CFQ),Self-Rating Anxiety Scale (SAS),Self-rating depression scale (SDS),and the Meaning in Life Questionnaire-the presence of meaning(MLQ-P).Variance analysis,pearson correlation analysis and stepwise multiple regression analysis were conducted in 157 questionnaires which were effective.Results The scores of AAQ-Ⅱ,CFQ,SAS,SDS,MLQ-P were(17.62±7.63),(27.37± 11.00),(32.38±6.99),(34.73±7.87),(28.55±5.09) respectively.The MLQ-P scores were negatively correlated with other scales(r=-0.278 ~-0.427,P<0.01).The correlations among AAQ-Ⅱ,CFQ,SAS,SDS were positive(r=0.359~0.784,P<0.01).The SAS scores and SDS scores in postoperative thyroid carcinoma patients were higher than that in norm group(z=72.28,P<0.01;z=2.25,P<0.05).When stepwise multiple regression analysis was conducted on SAS and SDS scores,AAQ-Ⅱ,MLQ-P,CFQ could explain 24.2% variance in anxiety,and AAQ-Ⅱ,MLQ-P could explain 24.4% variance in depression.Conclusion The level of anxiety and depression in postoperative thyroid carcinoma patients is much higher than that in norm.The higher the psychological flexibility,the lower the depression level.Psychological flexibility has significant explanatory power on anxiety and depression.
6.Expression and clinic significance of IGFBP-2 and IGFBP-6 in colorectal adenomas
Jing XUE ; Yang ZHAO ; Jianwei ZHANG ; Yan JIAO ; Xingbin SHEN
Chongqing Medicine 2015;(29):4112-4115
Objective To study the insulin‐like growth factor binding protein (IGFBP)‐2 and IGFBP‐6 expression and clini‐cal significance in colorectal adenomas .Methods A collection of Chengde Medical College Hospital from July 2012 to March 2013 after surgical treatment of colorectal cancer confirmed by pathology (colorectal cancer ,CRC) tissue samples of 50 patients ,colorec‐tal adenomas (colorectal adenoma ,CRA) 50 cases ,20 cases of colorectal normal mucosa .Immunohistochemistry and RT‐PCR were used to detect the expression of IGFBP‐2 and IGFBP‐6 protein and mRNA ,combined with clinical and pathological data were statis‐tically analyzed .Results IGFBP‐2 protein positive expression and the amount of mRNA expression in the CRA group compared with normal colorectal mucosa had a rising trend;While compared with CRC group had a tendency to reduce ,and the differences are obvious statistical significance (P<0 .05) ,and IGFBP‐6 protein positive expression in the CRA group compared with normal color‐ectal mucosa had a lower trend;While compared with CRC group amount IGFBP‐6 mRNA expression in the CRA group compared with normal colorectal mucosa had a rising trend;While compared with CRC group had a tendency to reduce ,and the differences were obvious statistical significance (P<0 .05) .In the CRA group ,IGFBP‐2 ,IGFBP‐6 positive expression and the patient′s age , sex ,tumor and the number of parts were no significant statistical difference (P>0 .05) ,but with the degree of hyperplasia had sig‐nificant statistical difference(P<0 .05);In the CRA group ,IGFBP‐2 and IGFBP‐6 expression were negatively related to each other , and the difference was statistically significant (P<0 .01) .Conclusion Colorectal adenomas in normal colorectal mucosa of colorec‐tal cancer to the middle part of the transformation process ,and in its occurrence and development process ,insulin‐like growth factor family (IGFs) and IGFS‐R axis plays an important and irreplaceable role ,so IGFBP‐2 ,IGFBP‐6 may be used as diagnostic colorectal adenomas and early predictors of prognosis ,clinical studies on colorectal adenomas is important .
7.The influence of anti-anxiety and depression treatment on quality life and plasma B-type natriuretic peptide for chronic heart failure patients
Jing YANG ; Xue YAO ; Xiaomeng WU ; Jie ZHANG
Chinese Journal of Postgraduates of Medicine 2014;37(19):53-56
Objective To investigate the influence of anti-anxiety and depression treatment on quality life and plasma B-type natriuretic peptide (BNP) for chronic heart failure (CHF) patients.Methods Eighty-two patients with CHF were divided into control group and intervention group by random digits table method with 41 cases each.After admission,the patients were given conventional anti-heart failure treatment.The patients in intervention group received the standard therapy in combination with lorazepam,with depression plus fluoxetine,and psychological treatment.After treatment,the patients were evaluated by symptom checklist (SCL-90),determined the plasma BNP level and heart function indexes,and recorded the length of stay,number of hospitalization,and so on.Results After treatment the intervention group somatization,interpersonal barriers as well as depression,anxiety,hostility,terrorism,paranoia,psychotic and other emotions significantly alleviated,there were statistical differences compared with before treatment and after treatment of control group (P < 0.05).There were no statistical differences in heart function indexes before treatment between the 2 groups (P > 0.05).After treatment in control group,only the left ventricular ejection fraction was significantly higher than that before treatment,there was statistical difference (P < 0.05).After treatment in intervention group compared with before treatment and after treatment in control group,the heart rate and left ventricular early diastolic and late peak velocity ratio were significantly lower [(71.0 ± 10.9) times/min vs.(82.5 ± 12.4) and (77.3 ± 10.1) times/min,1.26 ± 0.28 vs.1.38 ± 0.21 and 1.31 ± 0.33],left ventricular ejection fraction was significantly higher [(50.9 ± 6.9)% vs.(39.2 ± 7.4)% and (43.4 ± 7.8)%],there were statistical differences (P < 0.05).The plasma BNP level was downtrend after treatment in the 2 groups,compared with the control group,after 5 d of treatment in intervention group was significant decrease,the changes was slowly after 14 d of treatment.There were statistical differences in plasma BNP level in 5,7,10 d of treatment between the 2 groups (P < 0.05).The length of stay,number of hospitalization and fatality rate in intervention group were lower than those in control group [(6.0 ± 1.4)d/time vs.(10.0 ± 2.1) d/time,(1.6 ± 0.5) times/year vs.(3.2 ± 0.8) times/years,0 vs.9.8% (4/41)],there were statistical differences (P < 0.05).Conclusion Anti-anxiety and depression treatment can significantly improve cardiac function in CHF patients,shorter hospital stays,and improve quality of life.
8.Quality of life among Chinese women with breast cancer after modified radical mastectomy and immediate breast reconstruction:A comparative study
Yinqi GAO ; Yi FANG ; Xue YANG ; Jing WANG
Chinese Journal of Clinical Oncology 2014;45(11):729-733
Objective:This study was conducted to evaluate whether or not immediate breast reconstruction influences quality of life and patient body image satisfaction outcomes among Chinese women with breast cancer compared with modified radical mastecto-my. Methods:Sixty patients with breast cancer and were operated from July 2011 to July 2012 in Cancer Institute&Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College were included. These patients were divided into two groups:Ⅰ(30 patients) underwent modified radical mastectomy andⅡ(30 patients) underwent mastectomy with immediate breast reconstruction. Pa-tient satisfaction with breast reconstruction was evaluated by functionally assessing cancer therapy-breast cancer scale (FACT-B) and the scales that we designed. Results:No significant differences were observed between the two groups in terms of baseline characteris-tics, histopathological characteristics, and incision conditions (P>0.05). The patients in groupⅡ(immediate breast reconstruction) ex-hibited improved body image satisfaction outcomes (41.1 ± 10.8 vs. 33.2 ± 8.8, P=0.003);this difference was still observed after adjust-ment for confounding factors was performed by multiple linear regression. No significant difference was found between the two groups in terms of total FACT-B score and psychological evaluation score (P>0.05). Conclusion:Chinese women who prefer immediate breast reconstruction exhibited an improved body image satisfaction.
9.Value of virtual touch tissues quantization in the stages of type 2 diabetic nephropathy
Ning YU ; Xue YANG ; Chunhui LIU ; Zhengbin WANG ; Jing YU
Chinese Journal of Ultrasonography 2013;22(8):680-683
Objective To investigate the application value of virtual touch tissues quantization(VTQ)in the stages of type 2 diabetic nephropathy.Methods 120 cases of type 2 diabetes were divided into 3 groups according to urinary albumin excretion rate (UAER):diabetes without urinary albumin,diabetes with trace albuminuria and diabetes with mass albuminuria.The control group consisted 30 normal cases.VTQ was used to measure the renal shear wave velocity (SWV),then compare were made between the 4 groups.Besides,correlation analysis was made between SWV and UAER in diabetic nephropathy group.Results When compared SWV,there was no significant difference between diabetes without urinary albumin and control group (P =0.144),but there was significant difference between each 2 group of diabetes with trace albuminuria,diabetics with mass albuminuria group and control group (P < 0.05).There was also significant difference between each 2 group of diabetes (P < 0.05).The correlation coefficients for SWV and UAER in diabetes were 0.697(P <0.05).ROC analyses showed the area under curves for SWV diagnosis for diabetic nephropathy was 0.93.The best cutoff point,sensitivity and specificity were 2.43 m/s,85.7% and 84.5 %,respectively.Conclusions The renal SWV increases as the renal injury progresses,which also plays an important role in diabetic nephropathy stages.
10.Protective effects and its mechanism on neural cells after folic acid intervention in preeclampsia rat model
Jun WANG ; Jing GE ; Lina YANG ; Dan XUE ; Ju LI
Chinese Journal of Obstetrics and Gynecology 2011;46(8):605-609
Objective To investigate protective effects and mechanism of folic acid on brain neural cells in preeclampsia rat model.Methods Adult pregnant Wistar rats were randonly divided into 4 groups (n = 10 in each group).Rats in model group were injected intraperitoneally with homocysteine (Hcy,200 mg · kg-1 · d-1) daily and were injected subcutaneously every other day with monosodium glutamate (MSG,1 g · kg-1 · 48 h-1) from the 10th day of pregnancy to establish the model of preeclampsia. Lowdose folic acid (low dose group 10 ng · kg-1· d-1) and high-dose folic acid (high dose group 20 mg · kg-1 · d-1 ) were given intragastric administration with folic acid tablets dissolved in saline daily at the same time of establishing model.Rats in control group were injected or intragastric administration with the same dose of saline as above up to the 20th day of pregnancy.Brain tissue was fixed on the 20th day of pregnancy, so was that plasma folic acid was measured with automatic electro-chemiluminescence.Rats' immunohistochemical staining.bcl-2 mRNA and protein expression changes were observed by using reverse transcription(RT) -PCR and western blot.Results ( 1 ) Plasma folate concentrations were ( 39.5 ± 3.4 )nmol/L in low dose group and (40.1 ±5.4) nmol/L in high dose group, which were all significantly higher than (26.9 ± 6.7 ) nmol/L in model group( P < 0.01 ).Plasma folate in low dose and high dose group did not show significant difference( P > 0.05 ); ( 2 ) Apoptosis cell were 48.2 ± 9.1 in low dose group and 44.7 ±8.3 in high dose group, which were significantly lower than 75.8 ± 10.1 in model group (P<0.01).However, apoptosis cell in low dose and high dose group did not show significant difference( P >0.05 ) ;(3 )significant difference( P > 0.05 ); (4) bcl-2 mRNA and protein expression were 0.98 ± 0.49 and 0.89 ±0.52 in low dose group and 0.95 ± 0.38 and 0.92 ± 0.47 in high dose group which was significantly higher than 0.62 ± 0.20 and 0.45 ± 0.37 in model group ( P < 0.01 ); bcl-2 expression in low dose and high dose group showed no significant difference ( P > 0.05 ).Conclusions Folic acid has a protective role on neural activation and promoting bcl-2 gene and protein expression.